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RPL29 (NM_000992) Human Tagged ORF Clone
CAT＃: RC203616
RPL29 (Myc-DDK-tagged)-Human ribosomal protein L29 (RPL29)
ORF Plasmid: tGFP
"NM_000992" in other vectors (6)
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CNY 1,200.00
CNY 3,705.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HIP; HUMRPL29; L29; RPL29P10; RPL29_3_370 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC203616 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAAGTCCAAGAACCACACCACACACAACCAGTCCCGAAAATGGCACAGAAATGGTATCAAGAAAC CCCGATCACAAAGATACGAATCTCTTAAGGGGGTGGACCCCAAGTTCCTGAGGAACATGCGCTTTGCCAA GAAGCACAACAAAAAGGGCCTAAAGAAGATGCAGGCCAACAATGCCAAGGCCATGAGTGCACGTGCCGAG GCTATCAAGGCCCTCGTAAAGCCCAAGGAGGTTAAGCCCAAGATCCCAAAGGGTGTCAGCCGCAAGCTCG ATCGACTTGCCTACATTGCCCACCCCAAGCTTGGGAAGCGTGCTCGTGCCCGTATTGCCAAGGGGCTCAG GCTGTGCCGGCCAAAGGCCAAGGCCAAGGCCAAGGCCAAGGATCAAACCAAGGCCCAGGCTGCAGCCCCA GCTTCAGTTCCAGCTCAGGCTCCCAAACGTACCCAGGCCCCTACAAAGGCTTCAGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203616 protein sequence
Red=Cloning site Green=Tags(s) MAKSKNHTTHNQSRKWHRNGIKKPRSQRYESLKGVDPKFLRNMRFAKKHNKKGLKKMQANNAKAMSARAE AIKALVKPKEVKPKIPKGVSRKLDRLAYIAHPKLGKRARARIAKGLRLCRPKAKAKAKAKDQTKAQAAAP ASVPAQAPKRTQAPTKASE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000992 |
| ORF Size | 477 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000992.3 |
| RefSeq Size | 737 bp |
| RefSeq ORF | 480 bp |
| Locus ID | 6159 |
| UniProt ID | P47914 |
| Domains | Ribosomal_L29e |
| Protein Pathways | Ribosome |
| MW | 17.8 kDa |
| Gene Summary | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 60S subunit. The protein belongs to the L29E family of ribosomal proteins. The protein is also a peripheral membrane protein expressed on the cell surface that directly binds heparin. Although this gene was previously reported to map to 3q29-qter, it is believed that it is located at 3p21.3-p21.2. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Nascent chromatin capture proteomics determines chromatin dynamics during DNA replication and identifies unknown fork components
,Alabert, C;Bukowski-Wills, JC;Lee, SB;Kustatscher, G;Nakamura, K;de Lima Alves, F;Menard, P;Mejlvang, J;Rappsilber, J;Groth, A;,
Nat. Cell Biol., Feb 2014.
,PubMed ID 24561620
[RPL29]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203616L1 | Lenti ORF clone of Human ribosomal protein L29 (RPL29), Myc-DDK-tagged |
CNY 3,600.00 |
|
| RC203616L2 | Lenti ORF clone of Human ribosomal protein L29 (RPL29), mGFP tagged |
CNY 5,890.00 |
|
| RC203616L3 | Lenti ORF clone of Human ribosomal protein L29 (RPL29), Myc-DDK-tagged |
CNY 3,600.00 |
|
| RC203616L4 | Lenti ORF clone of Human ribosomal protein L29 (RPL29), mGFP tagged |
CNY 3,600.00 |
|
| RG203616 | RPL29 (tGFP-tagged) - Human ribosomal protein L29 (RPL29) |
CNY 2,800.00 |
|
| SC119499 | RPL29 (untagged)-Human ribosomal protein L29 (RPL29) |
CNY 1,200.00 |
