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CHRNA5 (NM_000745) Human Tagged ORF Clone
CAT＃: RC207624
- TrueORF®
CHRNA5 (Myc-DDK-tagged)-Human cholinergic receptor, nicotinic, alpha 5 (CHRNA5)
ORF Plasmid: tGFP
"NM_000745" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | LNCR2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207624 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGCGGGGGTCAGGGCCCCGCGCGCTCCGCCTGCTGCTCTTGGTCCAGCTGGTCGCGGGGCGCT GCGGTCTAGCGGGCGCGGCGGGCGGCGCGCAGAGAGGATTATCTGAACCTTCTTCTATTGCAAAACATGA AGATAGTTTGCTTAAGGATTTATTTCAAGACTACGAAAGATGGGTTCGTCCTGTGGAACACCTGAATGAC AAAATAAAAATAAAATTTGGACTTGCAATATCTCAATTGGTGGATGTGGATGAGAAAAATCAGTTAATGA CAACAAACGTCTGGTTGAAACAGGAATGGATAGATGTAAAATTAAGATGGAACCCTGATGACTATGGTGG AATAAAAGTTATACGTGTTCCTTCAGACTCTGTCTGGACACCAGACATCGTTTTGTTTGATAATGCAGAT GGACGTTTTGAAGGGACCAGTACGAAAACAGTCATCAGGTACAATGGCACTGTCACCTGGACTCCACCGG CAAACTACAAAAGTTCCTGTACCATAGATGTCACGTTTTTCCCATTTGACCTTCAGAACTGTTCCATGAA ATTTGGTTCTTGGACTTATGATGGATCACAGGTTGATATAATTCTAGAGGACCAAGATGTAGACAAGAGA GATTTTTTTGATAATGGAGAATGGGAGATTGTGAGTGCAACAGGGAGCAAAGGAAACAGAACCGACAGCT GTTGCTGGTATCCGTATGTCACTTACTCATTTGTAATCAAGCGCCTGCCTCTCTTTTATACCTTGTTCCT TATAATACCCTGTATTGGGCTCTCATTTTTAACTGTACTTGTCTTCTATCTTCCTTCAAATGAAGGTGAA AAGATTTGTCTCTGCACTTCAGTACTTGTGTCTTTGACTGTCTTCCTTCTGGTTATTGAAGAGATCATAC CATCATCTTCAAAAGTCATACCTCTAATTGGAGAGTATCTGGTATTTACCATGATTTTTGTGACACTGTC AATTATGGTAACCGTCTTCGCTATCAACATTCATCATCGTTCTTCCTCAACACATAATGCCATGGCGCCT TTGGTCCGCAAGATATTTCTTCACACGCTTCCCAAACTGCTTTGCATGAGAAGTCATGTAGACAGGTACT TCACTCAGAAAGAGGAAACTGAGAGTGGTAGTGGACCAAAATCTTCTAGAAACACATTGGAAGCTGCGCT CGATTCTATTCGCTACATTACAAGACACATCATGAAGGAAAATGATGTCCGTGAGGTTGTTGAAGATTGG AAATTCATAGCCCAGGTTCTTGATCGGATGTTTCTGTGGACTTTTCTTTTCGTTTCAATTGTTGGATCTC TTGGGCTTTTTGTTCCTGTTATTTATAAATGGGCAAATATATTAATACCAGTTCATATTGGAAATGCAAA TAAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC207624 protein sequence
Red=Cloning site Green=Tags(s) MAARGSGPRALRLLLLVQLVAGRCGLAGAAGGAQRGLSEPSSIAKHEDSLLKDLFQDYERWVRPVEHLND KIKIKFGLAISQLVDVDEKNQLMTTNVWLKQEWIDVKLRWNPDDYGGIKVIRVPSDSVWTPDIVLFDNAD GRFEGTSTKTVIRYNGTVTWTPPANYKSSCTIDVTFFPFDLQNCSMKFGSWTYDGSQVDIILEDQDVDKR DFFDNGEWEIVSATGSKGNRTDSCCWYPYVTYSFVIKRLPLFYTLFLIIPCIGLSFLTVLVFYLPSNEGE KICLCTSVLVSLTVFLLVIEEIIPSSSKVIPLIGEYLVFTMIFVTLSIMVTVFAINIHHRSSSTHNAMAP LVRKIFLHTLPKLLCMRSHVDRYFTQKEETESGSGPKSSRNTLEAALDSIRYITRHIMKENDVREVVEDW KFIAQVLDRMFLWTFLFVSIVGSLGLFVPVIYKWANILIPVHIGNANK SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000745 |
| ORF Size | 1404 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000745.3 |
| RefSeq Size | 3646 bp |
| RefSeq ORF | 1407 bp |
| Locus ID | 1138 |
| UniProt ID | P30532 |
| Domains | Neur_chan_memb, Neur_chan_LBD |
| Protein Families | Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane |
| MW | 53.1 kDa |
| Gene Summary | The protein encoded by this gene is a nicotinic acetylcholine receptor subunit and a member of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. These receptors are thought to be heteropentamers composed of separate but similar subunits. Defects in this gene have been linked to susceptibility to lung cancer type 2 (LNCR2).[provided by RefSeq, Jun 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207624L3 | Lenti ORF clone of Human cholinergic receptor, nicotinic, alpha 5 (CHRNA5), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207624L4 | Lenti ORF clone of Human cholinergic receptor, nicotinic, alpha 5 (CHRNA5), mGFP tagged |
CNY 5,890.00 |
|
| RG207624 | CHRNA5 (tGFP-tagged) - Human cholinergic receptor, nicotinic, alpha 5 (CHRNA5) |
CNY 4,370.00 |
|
| SC122164 | CHRNA5 (untagged)-Human cholinergic receptor, nicotinic, alpha 5 (CHRNA5) |
CNY 2,950.00 |
