Claudin 9 (CLDN9) (NM_020982) Human Tagged ORF Clone

CAT#: RC208701

  • TrueORF®

CLDN9 (Myc-DDK-tagged)-Human claudin 9 (CLDN9)

ORF Plasmid: DDK tGFP



  "NM_020982" in other vectors (6)

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CNY 3,600.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-CLDN9 Antibody
    • 100 ug

CNY 4,381.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms DFNB116
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC208701 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTTCGACCGGCTTAGAACTGCTGGGCATGACCCTGGCTGTGCTGGGCTGGCTGGGGACCCTGGTGT
CCTGCGCCCTGCCCCTGTGGAAGGTGACCGCCTTCATCGGCAACAGCATCGTGGTGGCCCAGGTGGTGTG
GGAGGGCCTGTGGATGTCCTGCGTGGTGCAGAGCACGGGCCAGATGCAGTGCAAGGTGTACGACTCACTG
CTGGCTCTGCCGCAGGACCTGCAGGCCGCACGTGCCCTCTGTGTCATTGCCCTCCTGCTGGCCCTGCTTG
GCCTCCTGGTGGCCATCACAGGTGCCCAGTGTACCACGTGTGTGGAGGACGAAGGTGCCAAGGCCCGTAT
CGTGCTCACCGCGGGGGTCATCCTCCTCCTCGCCGGCATCCTGGTGCTCATCCCTGTGTGCTGGACGGCG
CACGCCATCATCCAGGACTTCTACAACCCCCTGGTGGCTGAGGCCCTCAAGCGGGAGCTGGGGGCCTCCC
TCTACCTGGGCTGGGCGGCGGCTGCACTGCTTATGCTGGGCGGGGGGCTCCTCTGCTGCACGTGCCCCCC
GCCCCAGGTCGAGCGGCCCCGCGGACCTCGGCTGGGCTACTCCATCCCCTCCCGCTCGGGTGCATCTGGA
CTGGACAAGAGGGACTACGTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC208701 protein sequence
Red=Cloning site Green=Tags(s)

MASTGLELLGMTLAVLGWLGTLVSCALPLWKVTAFIGNSIVVAQVVWEGLWMSCVVQSTGQMQCKVYDSL
LALPQDLQAARALCVIALLLALLGLLVAITGAQCTTCVEDEGAKARIVLTAGVILLLAGILVLIPVCWTA
HAIIQDFYNPLVAEALKRELGASLYLGWAAAALLMLGGGLLCCTCPPPQVERPRGPRLGYSIPSRSGASG
LDKRDYV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_020982
ORF Size 651 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_020982.4
RefSeq Size 2139 bp
RefSeq ORF 654 bp
Locus ID 9080
UniProt ID O95484
Protein Families Transmembrane
Protein Pathways Cell adhesion molecules (CAMs), Leukocyte transendothelial migration, Tight junction
MW 22.8 kDa
Gene Summary This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This protein is one of the entry cofactors for hepatitis C virus. Mouse studies revealed that this gene is required for the preservation of sensory cells in the hearing organ and the gene deficiency is associated with deafness. [provided by RefSeq, Jun 2010]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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