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PRKAG2 (NM_024429) Human Tagged ORF Clone
CAT＃: RC209888
- TrueORF®
PRKAG2 (Myc-DDK-tagged)-Human protein kinase, AMP-activated, gamma 2 non-catalytic subunit (PRKAG2), transcript variant b
ORF Plasmid: tGFP
"NM_024429" in other vectors (4)
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CNY 3,990.00
Product images
CNY 300.00
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AAKG; AAKG2; CMH6; H91620p; WPWS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC209888 representing NM_024429
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGAGAAGCTGGAGTTCGAGGACGAAGCAGTAGAAGACTCAGAAAGTGGTGTTTACATGCGATTCA TGAGGTCACACAAGTGTTATGACATCGTTCCAACCAGTTCAAAGCTTGTTGTCTTTGATACTACATTACA AGTTAAAAAGGCCTTCTTTGCTTTGGTAGCCAACGGTGTCCGAGCAGCGCCACTGTGGGAGAGTAAAAAA CAAAGTTTTGTAGGAATGCTAACAATTACAGATTTCATAAATATACTACATAGATACTATAAATCACCTA TGGTACAGATTTATGAATTAGAGGAACATAAAATTGAAACATGGAGGGAGCTTTATTTACAAGAAACATT TAAGCCTTTAGTGAATATATCTCCAGATGCAAGCCTCTTCGATGCTGTATACTCCTTGATCAAAAATAAA ATCCACAGATTGCCCGTTATTGACCCTATCAGTGGGAATGCACTTTATATACTTACCCACAAAAGAATCC TCAAGTTCCTCCAGCTTTTTATGTCTGATATGCCAAAGCCTGCCTTCATGAAGCAGAACCTGGATGAGCT TGGAATAGGAACGTACCACAACATTGCCTTCATACATCCAGACACTCCCATCATCAAAGCCTTGAACATA TTTGTGGAAAGACGAATATCAGCTCTGCCTGTTGTGGATGAGTCAGGAAAAGTTGTAGATATTTATTCCA AATTTGATGTAATTAATCTTGCTGCTGAGAAAACATACAATAACCTAGATATCACGGTGACCCAGGCCCT TCAGCACCGTTCACAGTATTTTGAAGGTGTTGTGAAGTGCAATAAGCTGGAAATACTGGAGACCATCGTG GACAGAATAGTAAGAGCTGAGGTCCATCGGCTGGTGGTGGTAAATGAAGCAGATAGTATTGTGGGTATTA TTTCCCTGTCGGACATTCTGCAAGCCCTGATCCTCACACCAGCAGGTGCCAAACAAAAGGAGACAGAAAC GGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209888 representing NM_024429
Red=Cloning site Green=Tags(s) MLEKLEFEDEAVEDSESGVYMRFMRSHKCYDIVPTSSKLVVFDTTLQVKKAFFALVANGVRAAPLWESKK QSFVGMLTITDFINILHRYYKSPMVQIYELEEHKIETWRELYLQETFKPLVNISPDASLFDAVYSLIKNK IHRLPVIDPISGNALYILTHKRILKFLQLFMSDMPKPAFMKQNLDELGIGTYHNIAFIHPDTPIIKALNI FVERRISALPVVDESGKVVDIYSKFDVINLAAEKTYNNLDITVTQALQHRSQYFEGVVKCNKLEILETIV DRIVRAEVHRLVVVNEADSIVGIISLSDILQALILTPAGAKQKETETE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_024429 |
| ORF Size | 984 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_024429.1, NP_077747.1 |
| RefSeq Size | 2248 bp |
| RefSeq ORF | 987 bp |
| Locus ID | 51422 |
| UniProt ID | Q9UGJ0 |
| Protein Families | Druggable Genome |
| Protein Pathways | Adipocytokine signaling pathway, Hypertrophic cardiomyopathy (HCM), Insulin signaling pathway |
| MW | 37.5 kDa |
| Gene Summary | AMP-activated protein kinase (AMPK) is a heterotrimeric protein composed of a catalytic alpha subunit, a noncatalytic beta subunit, and a noncatalytic regulatory gamma subunit. Various forms of each of these subunits exist, encoded by different genes. AMPK is an important energy-sensing enzyme that monitors cellular energy status and functions by inactivating key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This gene is a member of the AMPK gamma subunit family. Mutations in this gene have been associated with Wolff-Parkinson-White syndrome, familial hypertrophic cardiomyopathy, and glycogen storage disease of the heart. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jan 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209888L3 | Lenti-ORF clone of PRKAG2 (Myc-DDK-tagged)-Human protein kinase, AMP-activated, gamma 2 non-catalytic subunit (PRKAG2), transcript variant b |
CNY 5,890.00 |
|
| RC209888L4 | Lenti-ORF clone of PRKAG2 (mGFP-tagged)-Human protein kinase, AMP-activated, gamma 2 non-catalytic subunit (PRKAG2), transcript variant b |
CNY 5,890.00 |
|
| RG209888 | PRKAG2 (tGFP-tagged) - Human protein kinase, AMP-activated, gamma 2 non-catalytic subunit (PRKAG2), transcript variant b |
CNY 4,370.00 |
|
| SC322089 | PRKAG2 (untagged)-Human protein kinase, AMP-activated, gamma 2 non-catalytic subunit (PRKAG2), transcript variant b |
CNY 2,400.00 |
