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WDR8 (WRAP73) (NM_017818) Human Tagged ORF Clone
CAT＃: RC210272
WRAP73 (Myc-DDK-tagged)-Human WD repeat containing, antisense to TP73 (WRAP73)
ORF Plasmid: tGFP
"NM_017818" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | WDR8 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210272 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTTCTCCGAGGTATTCAAGCTCTCCAGCTTACTCTGCAAGTTCTCCCCGGACGGCAAGTACCTGG CTTCCTGTGTCCAGTACCGGTTAGTGGTCCGGGATGTGAACACCCTTCAGATCCTTCAGCTGTACACGTG CCTAGACCAGATCCAGCACATCGAGTGGTCGGCAGACTCGCTCTTCATCCTGTGCGCCATGTACAAGCGA GGGCTGGTGCAGGTCTGGTCTTTAGAGCAGCCCGAATGGCACTGCAAAATAGACGAGGGCTCAGCCGGGC TGGTGGCCTCGTGCTGGAGCCCGGACGGGCGCCACATTCTCAACACCACGGAATTCCATCTGCGGATAAC CGTCTGGTCCTTGTGCACAAAATCCGTGTCTTACATCAAATACCCGAAAGCTTGTCTGCAGGGAATCACC TTCACCAGGGACGGCCGCTACATGGCGCTGGCAGAACGGCGCGACTGCAAAGATTACGTGAGCATCTTCG TCTGCAGTGATTGGCAGCTCCTGCGGCATTTTGATACGGACACCCAGGATCTCACAGGGATTGAGTGGGC CCCAAACGGCTGTGTGCTGGCAGTGTGGGACACCTGCTTGGAGTACAAGATTCTGCTGTACTCATTGGAT GGCCGGTTGTTGTCCACGTACAGCGCTTACGAGTGGTCCCTGGGCATCAAGTCTGTGGCCTGGAGCCCCA GCAGTCAGTTCCTGGCAGTTGGGAGCTATGATGGAAAGGTGCGCATCCTTAATCACGTGACTTGGAAAAT GATCACGGAGTTTGGGCATCCTGCAGCCATTAATGATCCCAAGATAGTGGTGTATAAGGAGGCCGAGAAG AGCCCACAGCTGGGACTGGGCTGCCTCTCCTTCCCGCCGCCCCGGGCCGGGGCCGGCCCTCTCCCGAGCT CAGAGAGTAAATATGAGATCGCCTCTGTCCCAGTCTCCTTACAGACACTGAAACCTGTTACCGACAGAGC AAACCCGAAAATCGGCATAGGAATGCTGGCATTTAGTCCTGACAGCTACTTCCTGGCGACAAGGAACGAC AACATTCCCAATGCCGTCTGGGTCTGGGACATTCAGAAGCTGAGGCTGTTCGCGGTGCTCGAGCAGCTGT CCCCAGTGCGCGCATTTCAGTGGGACCCGCAGCAGCCGCGGCTGGCCATCTGCACGGGAGGCAGCAGGCT CTACCTGTGGTCCCCAGCGGGCTGCATGTCGGTGCAGGTGCCTGGGGAAGGCGACTTTGCAGTGCTCTCT CTGTGCTGGCATTTAAGCGGAGACTCGATGGCCCTCCTCAGCAAGGATCACTTCTGCCTCTGCTTCCTGG AGACAGAGGCAGTGGTCGGCACAGCCTGCAGACAGCTGGGCGGCCACACG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210272 protein sequence
Red=Cloning site Green=Tags(s) MNFSEVFKLSSLLCKFSPDGKYLASCVQYRLVVRDVNTLQILQLYTCLDQIQHIEWSADSLFILCAMYKR GLVQVWSLEQPEWHCKIDEGSAGLVASCWSPDGRHILNTTEFHLRITVWSLCTKSVSYIKYPKACLQGIT FTRDGRYMALAERRDCKDYVSIFVCSDWQLLRHFDTDTQDLTGIEWAPNGCVLAVWDTCLEYKILLYSLD GRLLSTYSAYEWSLGIKSVAWSPSSQFLAVGSYDGKVRILNHVTWKMITEFGHPAAINDPKIVVYKEAEK SPQLGLGCLSFPPPRAGAGPLPSSESKYEIASVPVSLQTLKPVTDRANPKIGIGMLAFSPDSYFLATRND NIPNAVWVWDIQKLRLFAVLEQLSPVRAFQWDPQQPRLAICTGGSRLYLWSPAGCMSVQVPGEGDFAVLS LCWHLSGDSMALLSKDHFCLCFLETEAVVGTACRQLGGHT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017818 |
| ORF Size | 1380 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017818.4 |
| RefSeq Size | 1708 bp |
| RefSeq ORF | 1383 bp |
| Locus ID | 49856 |
| UniProt ID | Q9P2S5 |
| Domains | WD40 |
| Protein Families | Stem cell - Pluripotency |
| MW | 51.6 kDa |
| Gene Summary | This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Studies of the related mouse protein suggest that the encoded protein may play a role in the process of ossification. [provided by RefSeq, Mar 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210272L3 | Lenti ORF clone of Human WD repeat containing, antisense to TP73 (WRAP73), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210272L4 | Lenti ORF clone of Human WD repeat containing, antisense to TP73 (WRAP73), mGFP tagged |
CNY 5,890.00 |
|
| RG210272 | WRAP73 (tGFP-tagged) - Human WD repeat containing, antisense to TP73 (WRAP73) |
CNY 4,370.00 |
|
| SC113971 | WRAP73 (untagged)-Human WD repeat containing, antisense to TP73 (WRAP73) |
CNY 2,950.00 |
