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ZNF274 (NM_133502) Human Tagged ORF Clone
CAT＃: RC211914
ZNF274 (Myc-DDK-tagged)-Human zinc finger protein 274 (ZNF274), transcript variant ZNF274c
ORF Plasmid: tGFP
"NM_133502" in other vectors (4)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HFB101; ZF2; ZKSCAN19; ZSCAN51 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC211914 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCCAGGCTTCCGACGGCCTGGTCCTGTGAACCAGTGACCTTTGAAGATGTAACACTGGGTTTTA CCCCGGAAGAGTGGGGACTGCTGGACCTCAAACAGAAGTCCCTGTACAGGGAAGTGATGCTGGAGAACTA CAGGAACCTGGTCTCAGTGGAACATCAGCTTTCCAAACCAGATGTGGTATCTCAGTTAGAGGAGGCAGAA GATTTCTGGCCAGTGGAGAGAGGAATTCCTCAAGACACCATTCCAGAGTATCCTGAGCTCCAGCTGGACC CTAAATTGGATCCTCTTCCTGCTGAGAGTCCCCTAATGAACATTGAGGTTGTTGAGGTCCTCACACTGAA CCAGGAGGTGGCTGGTCCCCGGAATGCCCAGATCCAGGCCCTATATGCTGAAGATGGAAGCCTGAGTGCA GATGCCCCCAGTGAGCAGATCCAACAGCAGGGCAAGCATCCAGGTGACCCTGAGGCCGCGCGCCAGAGGT TCCGGCAGTTCCGTTATAAGGACATGACAGGTCCCCGGGAGGCCCTGGACCAGCTCCGAGAGCTGTGTCA CCAGTGGCTACAGCCTAAGGCACGCTCCAAGGAGCAGATCCTGGAGCTGCTGGTGCTGGAGCAGTTCCTA GGTGCACTGCCTGTGAAGCTCCGGACATGGGTGGAATCGCAGCACCCAGAGAACTGCCAAGAGGTGGTGG CCCTGGTAGAGGGTGTGACCTGGATGTCTGAGGAGGAAGTACTTCCTGCAGGACAACCTGCCGAGGGCAC CACCTGCTGCCTCGAGGTCACTGCCCAGCAGGAGGAGAAGCAGGAGGATGCAGCCATCTGCCCAGTGACA GTGCTCCCTGAGGAGCCAGTGACCTTCCAGGATGTGGCTGTGGACTTCAGCCGGGAGGAGTGGGGGCTGC TGGGCCCGACACAGAGGACCGAGTACCGCGATGTGATGCTGGAGACCTTTGGGCACCTGGTCTCTGTGGG GTGGGAGACTACACTGGAAAATAAAGAGTTAGCTCCAAATTCTGACATTCCTGAGGAAGAACCAGCCCCC AGCCTGAAAGTACAAGAATCCTCAAGGGATTGTGCCTTGTCCTCTACATTAGAAGATACCTTGCAGGGTG GGGTCCAGGAAGTCCAAGACACAGTGTTGAAGCAGATGGAGTCTGCTCAGGAAAAAGACCTTCCTCAGAA GAAGCACTTTGACAACCGTGAGTCCCAGGCAAACAGTGGTGCTCTTGACACAAACCAAGTTTCGCTCCAG AAAATTGACAACCCTGAGTCCCAGGCAAACAGTGGCGCTCTTGACACAAACCAAGTTTTGCTCCACAAAA TTCCTCCTAGAAAACGATTGCGCAAACGTGACTCACAAGTTAAAAGTATGAAACATAATTCACGTGTAAA AATTCATCAGAAGAGCTGTGAAAGGCAAAAGGCCAAGGAAGGCAATGGTTGTAGGAAAACCTTCAGTCGG AGTACTAAACAGATTACGTTTATAAGAATTCACAAGGGGAGCCAAGTTTGCCGATGCAGTGAATGTGGTA AAATATTCCGGAACCCAAGATACTTTTCTGTGCATAAGAAAATCCATACCGGAGAGAGGCCCTATGTGTG TCAAGACTGTGGGAAAGGATTTGTTCAGAGCTCTTCCCTCACACAGCATCAGAGAGTTCATTCTGGAGAG AGACCATTTGAATGTCAGGAGTGTGGGAGGACCTTCAATGATCGCTCAGCCATCTCCCAGCACCTGAGGA CTCACACTGGCGCTAAGCCCTACAAGTGTCAGGACTGTGGAAAAGCCTTCCGCCAGAGCTCCCACCTCAT CAGACATCAGAGGACTCACACCGGGGAGCGCCCATATGCATGCAACAAATGTGGAAAGGCCTTCACCCAG AGCTCACACCTTATTGGGCACCAGAGAACCCACAATAGGACAAAGCGAAAGAAGAAACAGCCTACCTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC211914 protein sequence
Red=Cloning site Green=Tags(s) MASRLPTAWSCEPVTFEDVTLGFTPEEWGLLDLKQKSLYREVMLENYRNLVSVEHQLSKPDVVSQLEEAE DFWPVERGIPQDTIPEYPELQLDPKLDPLPAESPLMNIEVVEVLTLNQEVAGPRNAQIQALYAEDGSLSA DAPSEQIQQQGKHPGDPEAARQRFRQFRYKDMTGPREALDQLRELCHQWLQPKARSKEQILELLVLEQFL GALPVKLRTWVESQHPENCQEVVALVEGVTWMSEEEVLPAGQPAEGTTCCLEVTAQQEEKQEDAAICPVT VLPEEPVTFQDVAVDFSREEWGLLGPTQRTEYRDVMLETFGHLVSVGWETTLENKELAPNSDIPEEEPAP SLKVQESSRDCALSSTLEDTLQGGVQEVQDTVLKQMESAQEKDLPQKKHFDNRESQANSGALDTNQVSLQ KIDNPESQANSGALDTNQVLLHKIPPRKRLRKRDSQVKSMKHNSRVKIHQKSCERQKAKEGNGCRKTFSR STKQITFIRIHKGSQVCRCSECGKIFRNPRYFSVHKKIHTGERPYVCQDCGKGFVQSSSLTQHQRVHSGE RPFECQECGRTFNDRSAISQHLRTHTGAKPYKCQDCGKAFRQSSHLIRHQRTHTGERPYACNKCGKAFTQ SSHLIGHQRTHNRTKRKKKQPTS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133502 |
| ORF Size | 1959 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133502.3 |
| RefSeq Size | 2890 bp |
| RefSeq ORF | 1962 bp |
| Locus ID | 10782 |
| UniProt ID | Q96GC6 |
| Domains | KRAB, LER, zf-C2H2 |
| Protein Families | Transcription Factors |
| Protein Pathways | Neurotrophin signaling pathway |
| MW | 74.2 kDa |
| Gene Summary | This gene encodes a zinc finger protein containing five C2H2-type zinc finger domains, one or two Kruppel-associated box A (KRAB A) domains, and a leucine-rich domain. The encoded protein has been suggested to be a transcriptional repressor. It localizes predominantly to the nucleolus. Alternatively spliced transcript variants encoding different isoforms exist. These variants utilize alternative polyadenylation signals. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211914L3 | Lenti ORF clone of Human zinc finger protein 274 (ZNF274), transcript variant ZNF274c, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC211914L4 | Lenti ORF clone of Human zinc finger protein 274 (ZNF274), transcript variant ZNF274c, mGFP tagged |
CNY 5,890.00 |
|
| RG211914 | ZNF274 (tGFP-tagged) - Human zinc finger protein 274 (ZNF274), transcript variant ZNF274c |
CNY 4,370.00 |
|
| SC124518 | ZNF274 (untagged)-Human zinc finger protein 274 (ZNF274), transcript variant ZNF274c |
CNY 2,950.00 |
