SPTBN1 (NM_003128) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	betaSpII; ELF; HEL102; SPTB2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC212868 representing NM_003128 Red=Cloning site Blue=ORF Green=Tags(s) CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC GCCC ATGACGACCACAGTAGCCACAGACTATGACAACATTGAGATCCAGCAGCAGTACAGTGATGTCAACAACC GCTGGGATGTCGACGACTGGGACAATGAGAACAGCTCTGCGCGGCTTTTTGAGCGGTCCCGCATCAAGGC TCTGGCAGATGAGCGTGAAGCCGTGCAGAAGAAGACCTTCACCAAGTGGGTCAATTCCCACCTTGCCCGT GTGTCCTGCCGGATCACAGACCTGTACACTGACCTTCGAGATGGACGGATGCTCATCAAGCTGCTGGAGG TCCTCTCTGGAGAGAGGCTGCCTAAACCCACCAAGGGACGAATGCGCATCCACTGCTTAGAGAATGTGGA CAAGGCCCTTCAGTTCCTGAAGGAGCAGAGAGTCCATCTTGAGAACATGGGGTCCCATGACATCGTGGAT GGAAACCACCGGCTGACCCTTGGCCTCATCTGGACCATCATCCTGCGCTTCCAGATCCAGGATATCAGTG TGGAAACTGAAGACAACAAAGAGAAGAAATCTGCCAAGGATGCATTGCTGTTGTGGTGCCAGATGAAGAC AGCTGGGTACCCCAATGTCAACATTCACAATTTCACCACTAGCTGGAGGGACGGCATGGCCTTCAATGCA CTGATACACAAACACCGGCCTGACCTGATAGATTTTGACAAACTAAAGAAATCTAACGCACACTACAACC TGCAGAATGCATTTAATCTGGCAGAACAGCACCTCGGCCTCACTAAACTGTTGGACCCCGAAGACATCAG CGTGGACCATCCTGATGAGAAGTCCATAATCACTTATGTGGTGACTTATTACCACTACTTCTCTAAGATG AAGGCCTTAGCTGTTGAAGGAAAACGAATTGGAAAGGTGCTTGACAATGCTATTGAAACAGAAAAAATGA TTGAAAAGTATGAATCACTTGCCTCTGACCTTCTGGAATGGATTGAACAAACCATCATCATTCTGAACAA TCGCAAATTTGCCAATTCACTGGTCGGGGTTCAACAGCAGCTTCAGGCATTCAACACTTACCGCACTGTG GAGAAACCACCCAAATTTACTGAGAAGGGGAACTTGGAAGTGCTGCTCTTCACCATTCAGAGCAAGATGA GGGCCAACAACCAGAAGGTCTACATGCCCCGGGAGGGGAAGCTCATCTCTGACATCAACAAGGCCTGGGA AAGACTGGAAAAAGCGGAACACGAAAGAGAACTGGCTTTGCGGAATGAGCTCATAAGACAGGAGAAACTG GAACAGCTCGCCCGCAGATTTGATCGCAAGGCAGCTATGAGGGAGACTTGGCTGAGCGAAAACCAGCGTC TGGTGTCTCAGGACAACTTTGGGTTTGACCTTCCTGCAGTTGAGGCCGCCACAAAAAAGCACGAGGCCAT TGAGACAGACATTGCCGCATACGAGGAGCGTGTGCAGGCTGTGGTAGCCGTGGCCAGGGAGCTCGAGGCC GAGAATTACCACGACATCAAGCGCATCACAGCGAGGAAGGACAATGTCATCCGGCTCTGGGAATACCTAC TGGAACTGCTCAGGGCCCGGAGACAGCGGCTCGAGATGAACCTGGGGCTGCAGAAGATATTCCAGGAAAT GCTCTACATTATGGACTGGATGGATGAAATGAAGGTGCTAGTATTGTCTCAAGACTATGGCAAACACTTA CTTGGTGTGGAAGACCTGTTACAGAAGCACACCCTGGTTGAAGCAGACATTGGCATCCAGGCAGAGCGGG TGAGAGGTGTCAATGCCTCCGCCCAGAAGTTCGCAACAGACGGGGAAGGTTACAAGCCCTGTGACCCCCA GGTGATCCGAGACCGCGTGGCCCACATGGAGTTCTGTTATCAAGAGCTTTGCCAGCTGGCGGCTGAGCGC AGGGCCCGTCTGGAAGAGTCCCGCCGCCTCTGGAAGTTCTTCTGGGAGATGGCAGAAGAGGAAGGCTGGA TACGGGAGAAGGAGAAGATCCTGTCCTCGGACGATTACGGGAAAGACCTGACCAGCGTCATGCGCCTGCT CAGCAAGCACCGGGCGTTCGAGGACGAGATGAGCGGCCGCAGTGGCCACTTTGAGCAGGCCATCAAGGAA GGCGAAGACATGATCGCGGAGGAGCACTTCGGGTCGGAGAAGATCCGTGAGAGGATCATTTACATCCGGG AGCAGTGGGCCAACCTAGAGCAGCTCTCGGCCATTCGGAAGAAGCGCCTGGAGGAGGCCTCCCTGCTGCA CCAGTTCCAGGCAGATGCTGATGACATTGATGCCTGGATGCTGGACATCCTCAAGATTGTCTCCAGCAGC GACGTGGGCCACGATGAGTATTCCACACAGTCTCTGGTCAAGAAACACAAGGACGTGGCGGAAGAGATCG CCAATTACAGGCCCACCCTTGACACGCTGCACGAACAAGCCAGCGCCCTCCCCCAGGAGCATGCCGAGTC TCCAGACGTGAGGGGCAGGCTGTCGGGCATCGAGGAGCGGTATAAGGAGGTGGCAGAGCTGACGCGGCTG CGGAAGCAGGCACTCCAGGACACTCTGGCCCTGTACAAGATGTTCAGCGAGGCTGATGCCTGTGAGCTCT GGATCGACGAGAAGGAGCAGTGGCTCAACAACATGCAGATCCCAGAGAAGCTGGAGGATCTGGAGGTCAT CCAGCACAGATTTGAGAGCCTAGAACCAGAAATGAACAACCAGGCTTCCCGGGTTGCAGTGGTGAACCAG ATTGCACGCCAGCTGATGCACAGCGGCCACCCAAGTGAGAAGGAAATCAAAGCCCAGCAGGACAAACTCA ACACAAGGTGGAGCCAGTTCAGAGAACTGGTTGACAGGAAGAAGGATGCCCTCCTGTCTGCCCTGAGCAT CCAGAACTACCACCTCGAGTGCAATGAAACCAAATCCTGGATTCGGGAAAAGACCAAGGTCATCGAGTCC ACCCAGGACCTGGGCAATGACCTGGCTGGCGTCATGGCCCTGCAGCGCAAGCTGACCGGCATGGAGCGGG ACTTGGTGGCCATTGAGGCAAAGCTGAGTGACCTGCAGAAGGAGGCGGAGAAGCTGGAGTCCGAGCACCC CGACCAGGCCCAGGCCATCCTGTCTCGGCTGGCCGAGATCAGCGACGTGTGGGAGGAGATGAAGACCACC CTGAAAAACCGAGAGGCCTCCCTGGGAGAGGCCAGCAAGCTGCAGCAGTTCCTACGGGACTTGGACGACT TCCAGTCCTGGCTCTCTAGGACCCAGACAGCGATCGCCTCGGAGGACATGCCAAACACCCTGACCGAGGC TGAGAAGCTGCTCACGCAGCACGAGAACATCAAGAATGAGATCGACAACTACGAGGAGGACTACCAGAAG ATGAGGGACATGGGCGAGATGGTCACCCAGGGGCAGACCGATGCCCAGTACATGTTTCTGCGGCAGCGGC TGCAGGCCCTGGACACTGGATGGAACGAGCTCCACAAGATGTGGGAGAACAGACAAAATCTCCTATCCCA GTCACATGCCTACCAGCAGTTCCTCAGAGACACGAAGCAAGCCGAAGCCTTTCTTAACAACCAGGAGTAT GTTCTGGCTCACACTGAAATGCCTACCACCTTGGAAGGAGCTGAAGCAGCAATTAAAAAGCAAGAGGACT TCATGACCACCATGGACGCCAATGAGGAGAAGATCAATGCTGTGGTGGAGACTGGCCGGAGGCTGGTGAG CGATGGGAACATCAACTCAGATCGCATCCAGGAGAAGGTGGACTCTATTGATGACAGACATAGGAAGAAT CGTGAGACAGCCAGTGAACTTTTGATGAGGTTGAAGGACAACAGGGATCTACAGAAATTCCTGCAAGATT GTCAAGAGCTGTCTCTCTGGATCAATGAGAAGATGCTCACAGCCCAGGACATGTCTTACGATGAAGCCAG AAATCTGCACAGTAAATGGTTGAAGCATCAAGCATTTATGGCAGAACTTGCATCCAACAAAGAATGGCTT GACAAAATCGAGAAGGAAGGAATGCAGCTCATTTCAGAAAAGCCTGAGACGGAAGCTGTGGTGAAGGAGA AACTCACTGGTTTACATAAAATGTGGGAAGTCCTTGAATCCACTACCCAGACAAAGGCCCAGCGGCTCTT TGATGCAAACAAGGCCGAACTTTTCACCCAGAGCTGTGCAGATCTAGACAAATGGCTGCACGGCCTGGAG AGTCAGATTCAGTCTGATGACTATGGCAAACACCTGACCAGTGTCAATATCCTGCTGAAAAAGCAACAGA TGCTGGAGAATCAGATGGAAGTGCGGAAGAAGGAGATCGAAGAGCTCCAAAGCCAAGCCCAGGCCCTGAG TCAGGAAGGGAAGAGCACCGACGAGGTAGACAGCAAGCGCCTCACCGTGCAGACCAAGTTCATGGAGTTG CTGGAGCCCTTGAACGAGAGGAAGCATAACCTGCTGGCCTCCAAAGAGATCCATCAGTTCAACAGGGATG TGGAGGACGAGATCTTGTGGGTTGGAGAGAGGATGCCTTTGGCAACTTCCACGGATCATGGCCACAACCT CCAGACTGTGCAGCTGTTAATAAAGAAAAATCAGACCCTCCAGAAAGAAATCCAGGGGCACCAGCCTCGC ATTGACGACATCTTTGAGAGGAGCCAAAACATCGTCACTGACAGCAGCAGCCTCAGCGCTGAGGCCATCA GACAGAGGCTTGCCGACCTGAAGCAGCTGTGGGGTCTCCTCATTGAGGAGACAGAGAAACGCCACAGGCG GCTGGAGGAGGCGCACAGGGCCCAGCAGTACTACTTTGACGCTGCTGAGGCCGAAGCCTGGATGAGCGAG CAGGAGCTGTACATGATGTCAGAGGAGAAGGCCAAGGATGAGCAGAGTGCTGTCTCCATGTTGAAGAAGC ACCAGATCTTAGAACAAGCTGTGGAGGACTATGCAGAGACCGTGCATCAGCTCTCCAAGACCAGCCGGGC CCTGGTGGCCGACAGCCATCCTGAAAGTGAGCGCATTAGCATGCGGCAGTCCAAAGTGGATAAACTGTAC GCTGGTCTGAAAGACCTTGCTGAAGAGAGAAGAGGCAAGCTGGATGAGAGACACAGGTTATTCCAGCTCA ACCGGGAGGTGGACGACCTGGAGCAGTGGATCGCTGAGAGGGAGGTGGTCGCAGGGTCCCATGAACTGGG ACAGGACTATGAGCATGTCACGATGTTACAAGAACGATTCCGGGAGTTTGCCCGAGACACCGGGAACATT GGGCAGGAGCGCGTGGACACGGTCAATCACCTGGCAGATGAGCTCATCAACTCTGGACATTCAGATGCCG CCACCATCGCTGAATGGAAGGATGGCCTCAATGAAGCCTGGGCCGACCTCCTGGAGCTCATTGACACAAG AACACAGATTCTTGCCGCTTCCTATGAACTGCACAAGTTTTACCACGATGCCAAGGAGATCTTTGGGCGT ATACAGGACAAACACAAGAAACTCCCTGAGGAGCTTGGGAGAGATCAGAACACAGTGGAGACCTTACAGA GAATGCACACTACATTTGAGCATGACATCCAGGCTCTGGGCACACAGGTGAGGCAGCTGCAGGAGGATGC AGCCCGCCTCCAGGCGGCCTATGCGGGTGACAAGGCCGACGATATCCAGAAGCGCGAGAACGAGGTCCTG GAAGCCTGGAAGTCCCTCCTGGACGCCTGTGAGAGCCGCAGGGTGCGGCTGGTGGACACAGGGGACAAGT TCCGCTTCTTCAGCATGGTGCGCGACCTCATGCTCTGGATGGAGGATGTCATCCGGCAGATCGAGGCCCA GGAGAAGCCAAGGGATGTATCATCTGTTGAACTCTTAATGAATAATCATCAAGGCATCAAAGCTGAAATT GATGCACGTAATGACAGTTTCACAACCTGCATTGAACTTGGGAAATCCCTGTTGGCGAGAAAACACTATG CATCTGAGGAGATCAAGGAAAAATTACTGCAGTTGACGGAAAAGAGGAAAGAAATGATCGACAAGTGGGA AGACCGATGGGAATGGTTAAGACTGATTCTGGAGGTCCATCAGTTCTCAAGAGACGCCAGTGTGGCCGAG GCCTGGCTGCTTGGACAGGAGCCGTACCTATCCAGCCGAGAGATAGGCCAGAGCGTGGACGAGGTGGAGA AGCTCATCAAGCGCCACGAGGCATTTGAAAAGTCTGCAGCAACCTGGGATGAGAGGTTCTCTGCCCTGGA AAGGCTGACTACATTGGAGTTACTGGAAGTGCGCAGACAGCAAGAGGAAGAGGAGAGGAAGAGGCGGCCG CCTTCTCCCGAGCCGAGCACGAAGGTTTCAGAGGAAGCCGAGTCCCAGCAGCAGTGGGATACTTCAAAAG GAGAACAAGTTTCCCAAAACGGTTTGCCAGCTGAACAGGGATCTCCACGGATGGCAGAAACGGTGGACAC AAGCGAAATGGTCAACGGCGCTACAGAACAAAGGACGAGCTCTAAAGAGTCCAGCCCCATCCCCTCCCCG ACCTCTGATCGTAAAGCCAAGACTGCCCTCCCAGCCCAGAGTGCCGCCACCTTACCAGCCAGAACCCAGG AGACACCTTCGGCCCAGATGGAAGGCTTCCTCAATCGGAAACACGAGTGGGAGGCCCACAATAAGAAAGC CTCAAGCAGGTCCTGGCACAATGTTTATTGTGTCATAAATAACCAAGAAATGGGTTTCTACAAAGATGCA AAGACTGCTGCTTCTGGAATTCCCTACCACAGCGAGGTCCCTGTGAGTTTGAAAGAAGCTGTCTGCGAAG TGGCCCTTGATTACAAAAAGAAGAAACACGTATTCAAGCTAAGACTAAATGATGGCAATGAGTACCTCTT CCAAGCCAAAGACGATGAGGAAATGAACACATGGATCCAGGCTATCTCTTCCGCCATCTCCTCTGATAAA CACGAGGTGTCTGCCAGCACCCAGAGCACGCCAGCATCCAGCCGCGCGCAGACCCTCCCCACCAGCGTCG TCACCATCACCAGCGAGTCCAGTCCCGGCAAGCGGGAAAAGGACAAAGAGAAAGACAAAGAGAAGCGGTT CAGCCTTTTTGGCAAAAAGAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC212868 representing NM_003128 Red=Cloning site Green=Tags(s) MTTTVATDYDNIEIQQQYSDVNNRWDVDDWDNENSSARLFERSRIKALADEREAVQKKTFTKWVNSHLAR VSCRITDLYTDLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVD GNHRLTLGLIWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNIHNFTTSWRDGMAFNA LIHKHRPDLIDFDKLKKSNAHYNLQNAFNLAEQHLGLTKLLDPEDISVDHPDEKSIITYVVTYYHYFSKM KALAVEGKRIGKVLDNAIETEKMIEKYESLASDLLEWIEQTIIILNNRKFANSLVGVQQQLQAFNTYRTV EKPPKFTEKGNLEVLLFTIQSKMRANNQKVYMPREGKLISDINKAWERLEKAEHERELALRNELIRQEKL EQLARRFDRKAAMRETWLSENQRLVSQDNFGFDLPAVEAATKKHEAIETDIAAYEERVQAVVAVARELEA ENYHDIKRITARKDNVIRLWEYLLELLRARRQRLEMNLGLQKIFQEMLYIMDWMDEMKVLVLSQDYGKHL LGVEDLLQKHTLVEADIGIQAERVRGVNASAQKFATDGEGYKPCDPQVIRDRVAHMEFCYQELCQLAAER RARLEESRRLWKFFWEMAEEEGWIREKEKILSSDDYGKDLTSVMRLLSKHRAFEDEMSGRSGHFEQAIKE GEDMIAEEHFGSEKIRERIIYIREQWANLEQLSAIRKKRLEEASLLHQFQADADDIDAWMLDILKIVSSS DVGHDEYSTQSLVKKHKDVAEEIANYRPTLDTLHEQASALPQEHAESPDVRGRLSGIEERYKEVAELTRL RKQALQDTLALYKMFSEADACELWIDEKEQWLNNMQIPEKLEDLEVIQHRFESLEPEMNNQASRVAVVNQ IARQLMHSGHPSEKEIKAQQDKLNTRWSQFRELVDRKKDALLSALSIQNYHLECNETKSWIREKTKVIES TQDLGNDLAGVMALQRKLTGMERDLVAIEAKLSDLQKEAEKLESEHPDQAQAILSRLAEISDVWEEMKTT LKNREASLGEASKLQQFLRDLDDFQSWLSRTQTAIASEDMPNTLTEAEKLLTQHENIKNEIDNYEEDYQK MRDMGEMVTQGQTDAQYMFLRQRLQALDTGWNELHKMWENRQNLLSQSHAYQQFLRDTKQAEAFLNNQEY VLAHTEMPTTLEGAEAAIKKQEDFMTTMDANEEKINAVVETGRRLVSDGNINSDRIQEKVDSIDDRHRKN RETASELLMRLKDNRDLQKFLQDCQELSLWINEKMLTAQDMSYDEARNLHSKWLKHQAFMAELASNKEWL DKIEKEGMQLISEKPETEAVVKEKLTGLHKMWEVLESTTQTKAQRLFDANKAELFTQSCADLDKWLHGLE SQIQSDDYGKHLTSVNILLKKQQMLENQMEVRKKEIEELQSQAQALSQEGKSTDEVDSKRLTVQTKFMEL LEPLNERKHNLLASKEIHQFNRDVEDEILWVGERMPLATSTDHGHNLQTVQLLIKKNQTLQKEIQGHQPR IDDIFERSQNIVTDSSSLSAEAIRQRLADLKQLWGLLIEETEKRHRRLEEAHRAQQYYFDAAEAEAWMSE QELYMMSEEKAKDEQSAVSMLKKHQILEQAVEDYAETVHQLSKTSRALVADSHPESERISMRQSKVDKLY AGLKDLAEERRGKLDERHRLFQLNREVDDLEQWIAEREVVAGSHELGQDYEHVTMLQERFREFARDTGNI GQERVDTVNHLADELINSGHSDAATIAEWKDGLNEAWADLLELIDTRTQILAASYELHKFYHDAKEIFGR IQDKHKKLPEELGRDQNTVETLQRMHTTFEHDIQALGTQVRQLQEDAARLQAAYAGDKADDIQKRENEVL EAWKSLLDACESRRVRLVDTGDKFRFFSMVRDLMLWMEDVIRQIEAQEKPRDVSSVELLMNNHQGIKAEI DARNDSFTTCIELGKSLLARKHYASEEIKEKLLQLTEKRKEMIDKWEDRWEWLRLILEVHQFSRDASVAE AWLLGQEPYLSSREIGQSVDEVEKLIKRHEAFEKSAATWDERFSALERLTTLELLEVRRQQEEEERKRRP PSPEPSTKVSEEAESQQQWDTSKGEQVSQNGLPAEQGSPRMAETVDTSEMVNGATEQRTSSKESSPIPSP TSDRKAKTALPAQSAATLPARTQETPSAQMEGFLNRKHEWEAHNKKASSRSWHNVYCVINNQEMGFYKDA KTAASGIPYHSEVPVSLKEAVCEVALDYKKKKHVFKLRLNDGNEYLFQAKDDEEMNTWIQAISSAISSDK HEVSASTQSTPASSRAQTLPTSVVTITSESSPGKREKDKEKDKEKRFSLFGKKK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	AscI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_003128
ORF Size	7092 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_003128.1, NP_003119.1
RefSeq Size	7561 bp
RefSeq ORF	7095 bp
Locus ID	6711
UniProt ID	Q01082
Domains	CH, PH, spectrin
MW	274.4 kDa
Gene Summary	Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. It is composed of two antiparallel dimers of alpha- and beta- subunits. This gene is one member of a family of beta-spectrin genes. The encoded protein contains an N-terminal actin-binding domain, and 17 spectrin repeats which are involved in dimer formation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

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