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ATP1A4 (NM_144699) Human Tagged ORF Clone
CAT＃: RC213903
- TrueORF®
ATP1A4 (Myc-DDK-tagged)-Human ATPase, Na+/K+ transporting, alpha 4 polypeptide (ATP1A4), transcript variant 1
ORF Plasmid: tGFP
"NM_144699" in other vectors (4)
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CNY 10,260.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ATP1A1; ATP1AL2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC213903 representing NM_144699
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCTTTGGGGGAAGAAAGGGACAGTGGCTCCCCATGACCAGAGTCCAAGACGAAGACCTAAAAAAG GGCTTATCAAGAAAAAAATGGTGAAGAGGGAAAAACAGAAGCGCAATATGGAGGAACTGAAGAAGGAAGT GGTCATGGATGATCACAAATTAACCTTGGAAGAGCTGAGCACCAAGTACTCCGTGGACCTGACAAAGGGC CATAGCCACCAAAGGGCAAAGGAAATCCTGACTCGAGGTGGACCCAATACTGTTACCCCACCCCCCACCA CTCCAGAATGGGTCAAATTCTGTAAGCAACTGTTCGGAGGCTTCTCCCTCCTACTATGGACTGGGGCCAT TCTCTGCTTTGTGGCCTACAGCATCCAGATATATTTCAATGAGGAGCCTACCAAAGACAACCTCTACCTG AGCATCGTACTGTCCGTCGTGGTCATCGTCACTGGCTGCTTCTCCTATTATCAGGAGGCCAAGAGCTCCA AGATCATGGAGTCTTTTAAGAACATGGTGCCTCAGCAAGCTCTGGTAATTCGAGGAGGAGAGAAGATGCA AATTAATGTACAAGAGGTGGTGTTGGGAGACCTGGTGGAAATCAAGGGTGGAGACCGAGTCCCTGCTGAC CTCCGGCTTATCTCTGCACAAGGATGTAAGGTGGACAACTCATCCTTGACTGGGGAGTCAGAACCCCAGA GCCGCTCCCCTGACTTCACCCATGAGAACCCTCTGGAGACCCGAAACATCTGCTTCTTTTCCACCAACTG TGTGGAAGGAACCGCCCGGGGTATTGTGATTGCTACGGGAGACTCCACAGTGATGGGCAGAATTGCCTCC CTGACGTCAGGCCTGGCGGTTGGCCAGACACCTATCGCTGCTGAGATCGAACACTTCATCCATCTGATCA CTGTGGTGGCCGTCTTCCTTGGTGTCACTTTTTTTGCGCTCTCACTTCTCTTGGGCTATGGTTGGCTGGA GGCTATCATTTTTCTCATTGGCATCATTGTGGCCAATGTGCCTGAGGGGCTGTTGGCCACAGTCACTGTG TGCCTGACCCTCACAGCCAAGCGCATGGCGCGGAAGAACTGCCTGGTGAAGAACCTGGAGGCGGTGGAGA CGCTGGGCTCCACGTCCACCATCTGCTCAGACAAGACGGGCACCCTCACCCAGAACCGCATGACCGTCGC CCACATGTGGTTTGATATGACCGTGTATGAGGCCGACACCACTGAAGAACAGACTGGAAAAACATTTACC AAGAGCTCTGATACCTGGTTTATGCTGGCCCGAATCGCTGGCCTCTGCAACCGGGCTGACTTTAAGGCTA ATCAGGAGATCCTGCCCATTGCTAAGAGGGCCACAACAGGTGATGCTTCCGAGTCAGCCCTCCTCAAGTT CATCGAGCAGTCTTACAGCTCTGTGGCGGAGATGAGAGAGAAAAACCCCAAGGTGGCAGAGATTCCCTTT AATTCTACCAACAAGTACCAGATGTCCATCCACCTTCGGGAGGACAGCTCCCAGACCCACGTACTGATGA TGAAGGGTGCTCCGGAGAGGATCTTGGAGTTTTGTTCTACCTTTCTTCTGAATGGGCAGGAGTACTCAAT GAACGATGAAATGAAGGAAGCCTTCCAAAATGCCTACTTAGAACTGGGAGGTCTGGGGGAACGTGTGCTA GGCTTCTGCTTCTTGAATCTGCCTAGCAGCTTCTCCAAGGGATTCCCATTTAATACAGATGAAATAAATT TCCCCATGGACAACCTTTGTTTTGTGGGCCTCATATCCATGATTGACCCTCCCCGAGCTGCAGTGCCTGA TGCTGTGAGCAAGTGTCGCAGTGCAGGAATTAAGGTGATCATGGTAACAGGAGATCATCCCATTACAGCT AAGGCCATTGCCAAGGGTGTGGGCATCATCTCAGAAGGCACTGAGACGGCAGAGGAAGTCGCTGCCCGGC TTAAGATCCCTATCAGCAAGGTCGATGCCAGTGCTGCCAAAGCCATTGTGGTGCATGGTGCAGAACTGAA GGACATACAGTCCAAGCAGCTTGATCAGATCCTCCAGAACCACCCTGAGATCGTGTTTGCTCGGACCTCC CCTCAGCAGAAGCTCATCATTGTCGAGGGATGTCAGAGGCTGGGAGCCGTTGTGGCCGTGACAGGTGACG GGGTGAACGACTCCCCTGCGCTGAAGAAGGCTGACATTGGCATTGCCATGGGCATCTCTGGCTCTGACGT CTCTAAGCAGGCAGCCGACATGATCCTGCTGGATGACAACTTTGCCTCCATCGTCACGGGGGTGGAGGAG GGCCGCCTGATCTTTGACAACCTGAAGAAATCCATCATGTACACCCTGACCAGCAACATCCCCGAGATCA CGCCCTTCCTGATGTTCATCATCCTCGGTATACCCCTGCCTCTGGGAACCATAACCATCCTCTGCATTGA TCTCGGCACTGACATGGTCCCTGCCATCTCCTTGGCTTATGAGTCAGCTGAAAGCGACATCATGAAGAGG CTTCCAAGGAACCCAAAGACGGATAATCTGGTGAACCACCGTCTCATTGGCATGGCCTATGGACAGATTG GGATGATCCAGGCTCTGGCTGGATTCTTTACCTACTTTGTAATCCTGGCTGAGAATGGTTTTAGGCCTGT TGATCTGCTGGGCATCCGCCTCCACTGGGAAGATAAATACTTGAATGACCTGGAGGACAGCTACGGACAG CAGTGGACCTATGAGCAACGAAAAGTTGTGGAGTTCACATGCCAAACGGCCTTTTTTGTCACCATCGTGG TTGTGCAGTGGGCGGATCTCATCATCTCCAAGACTCGCCGCAACTCACTTTTCCAGCAGGGCATGAGAAA CAAAGTCTTAATATTTGGGATCCTGGAGGAGACACTCTTGGCTGCATTTCTGTCCTACACTCCAGGCATG GACGTGGCCCTGCGAATGTACCCACTCAAGATAACCTGGTGGCTCTGTGCCATTCCCTACAGTATTCTCA TCTTCGTCTATGATGAAATCAGAAAACTCCTCATCCGTCAGCACCCGGATGGCTGGGTGGAAAGGGAGAC GTACTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC213903 representing NM_144699
Red=Cloning site Green=Tags(s) MGLWGKKGTVAPHDQSPRRRPKKGLIKKKMVKREKQKRNMEELKKEVVMDDHKLTLEELSTKYSVDLTKG HSHQRAKEILTRGGPNTVTPPPTTPEWVKFCKQLFGGFSLLLWTGAILCFVAYSIQIYFNEEPTKDNLYL SIVLSVVVIVTGCFSYYQEAKSSKIMESFKNMVPQQALVIRGGEKMQINVQEVVLGDLVEIKGGDRVPAD LRLISAQGCKVDNSSLTGESEPQSRSPDFTHENPLETRNICFFSTNCVEGTARGIVIATGDSTVMGRIAS LTSGLAVGQTPIAAEIEHFIHLITVVAVFLGVTFFALSLLLGYGWLEAIIFLIGIIVANVPEGLLATVTV CLTLTAKRMARKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDMTVYEADTTEEQTGKTFT KSSDTWFMLARIAGLCNRADFKANQEILPIAKRATTGDASESALLKFIEQSYSSVAEMREKNPKVAEIPF NSTNKYQMSIHLREDSSQTHVLMMKGAPERILEFCSTFLLNGQEYSMNDEMKEAFQNAYLELGGLGERVL GFCFLNLPSSFSKGFPFNTDEINFPMDNLCFVGLISMIDPPRAAVPDAVSKCRSAGIKVIMVTGDHPITA KAIAKGVGIISEGTETAEEVAARLKIPISKVDASAAKAIVVHGAELKDIQSKQLDQILQNHPEIVFARTS PQQKLIIVEGCQRLGAVVAVTGDGVNDSPALKKADIGIAMGISGSDVSKQAADMILLDDNFASIVTGVEE GRLIFDNLKKSIMYTLTSNIPEITPFLMFIILGIPLPLGTITILCIDLGTDMVPAISLAYESAESDIMKR LPRNPKTDNLVNHRLIGMAYGQIGMIQALAGFFTYFVILAENGFRPVDLLGIRLHWEDKYLNDLEDSYGQ QWTYEQRKVVEFTCQTAFFVTIVVVQWADLIISKTRRNSLFQQGMRNKVLIFGILEETLLAAFLSYTPGM DVALRMYPLKITWWLCAIPYSILIFVYDEIRKLLIRQHPDGWVERETYY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_144699 |
| ORF Size | 3087 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_144699.4 |
| RefSeq Size | 3912 bp |
| RefSeq ORF | 3090 bp |
| Locus ID | 480 |
| UniProt ID | Q13733 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Cardiac muscle contraction |
| MW | 114.2 kDa |
| Gene Summary | The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 4 subunit. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213903L3 | Lenti-ORF clone of ATP1A4 (Myc-DDK-tagged)-Human ATPase, Na+/K+ transporting, alpha 4 polypeptide (ATP1A4), transcript variant 1 |
CNY 12,160.00 |
|
| RC213903L4 | Lenti-ORF clone of ATP1A4 (mGFP-tagged)-Human ATPase, Na+/K+ transporting, alpha 4 polypeptide (ATP1A4), transcript variant 1 |
CNY 12,160.00 |
|
| RG213903 | ATP1A4 (tGFP-tagged) - Human ATPase, Na+/K+ transporting, alpha 4 polypeptide (ATP1A4), transcript variant 1 |
CNY 9,480.00 |
|
| SC306187 | ATP1A4 (untagged)-Human ATPase, Na+/K+ transporting, alpha 4 polypeptide (ATP1A4), transcript variant 1 |
CNY 10,450.00 |
