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UBA6 (NM_018227) Human Tagged ORF Clone
CAT＃: RC214483
UBA6 (Myc-DDK-tagged)-Human ubiquitin-like modifier activating enzyme 6 (UBA6)
ORF Plasmid: tGFP
"NM_018227" in other vectors (7)
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CNY 7,328.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | E1-L2; MOP-4; UBE1L2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC214483 representing NM_018227
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGGATCCGAGCCTGTGGCCGCCCATCAGGGGGAAGAGGCGTCCTGTTCTTCCTGGGGGACTGGCA GCACAAATAAAAATTTGCCCATTATGTCAACAGCATCTGTGGAAATCGATGATGCATTGTATAGTCGACA GAGGTACGTTCTTGGAGACACAGCAATGCAGAAGATGGCCAAGTCCCATGTTTTCTTAAGTGGGATGGGT GGTCTTGGTTTGGAAATTGCAAAGAATCTTGTTCTTGCAGGGATTAAGGCAGTTACAATTCATGATACAG AAAAATGCCAAGCATGGGATCTAGGAACCAACTTCTTTCTCAGTGAAGATGATGTTGTTAATAAGAGAAA CAGGGCTGAAGCTGTACTTAAACATATTGCAGAACTAAATCCATACGTTCATGTCACATCATCTTCTGTT CCTTTCAATGAGACCACAGATCTCTCCTTTTTAGATAAATACCAGTGTGTAGTATTGACTGAGATGAAAC TTCCATTGCAGAAGAAGATCAATGACTTTTGCCGTTCTCAGTGCCCTCCAATTAAGTTTATCAGTGCAGA TGTACATGGAATTTGGTCAAGGTTATTTTGTGATTTCGGTGATGAATTTGAAGTTTTAGATACAACAGGA GAAGAACCAAAAGAAATTTTCATTTCAAACATAACGCAAGCAAATCCTGGCATTGTTACTTGCCTTGAAA ATCATCCTCACAAACTGGAGACAGGACAATTCCTAACATTTCGAGAAATTAATGGAATGACAGGTTTAAA TGGATCTATACAACAAATAACGGTGATATCGCCATTTTCTTTTAGTATTGGTGACACCACAGAACTGGAA CCATATTTACATGGAGGCATAGCTGTCCAAGTTAAGACTCCTAAAACAGTTTTTTTTGAATCACTGGAGA GGCAGTTAAAACATCCAAAGTGCCTTATTGTGGATTTTAGCAACCCTGAGGCACCTTTAGAGATTCACAC AGCTATGCTTGCCTTGGACCAGTTTCAGGAGAAATACAGTCGCAAGCCAAATGTTGGATGCCAACAAGAT TCAGAAGAACTGTTGAAACTAGCAACATCTATAAGTGAAACCTTGGAAGAGAAGCCTGATGTAAATGCTG ACATTGTGCATTGGCTCTCTTGGACTGCCCAAGGCTTTTTATCTCCACTTGCTGCAGCAGTAGGAGGTGT TGCCAGCCAAGAAGTATTGAAAGCTGTAACAGGAAAATTTTCTCCTTTGTGCCAGTGGTTATATCTTGAA GCAGCAGATATTGTTGAATCACTAGGCAAACCTGAATGTGAAGAATTTCTCCCACGAGGAGATAGATATG ATGCCTTAAGAGCTTGCATTGGAGACACTTTGTGTCAGAAACTGCAAAATTTAAACATCTTCTTAGTAGG GTGTGGAGCCATAGGCTGTGAAATGTTGAAAAATTTTGCTTTACTTGGTGTTGGCACAAGCAAAGAGAAA GGAATGATTACAGTTACAGATCCTGACTTGATAGAGAAATCCAACTTAAATAGACAGTTCCTATTTCGTC CTCATCACATACAGAAACCTAAAAGCTACACTGCTGCTGATGCTACTCTGAAAATAAATTCTCAAATAAA GATAGATGCACACCTGAACAAAGTATGTCCAACCACTGAGACCATTTACAATGATGAGTTCTATACTAAA CAAGATGTAATTATTACAGCATTAGATAATGTGGAAGCCAGGAGATACGTAGACAGTCGTTGCTTAGCAA ATCTAAGGCCTCTTTTAGATTCTGGAACAATGGGCACTAAGGGACACACTGAAGTTATTGTACCGCATTT GACTGAGTCTTACAATAGTCATCGGGATCCCCCAGAAGAGGAAATACCATTTTGTACTCTAAAATCCTTT CCAGCTGCTATTGAACATACCATACAGTGGGCAAGAGATAAGTTTGAAAGTTCCTTTTCCCACAAACCTT CATTGTTTAACAAATTTTGGCAAACCTATTCATCTGCAGAAGAAGTCTTACAGAAGATACAGAGTGGACA CAGTTTAGAAGGCTGTTTTCAAGTTATAAAGTTACTTAGCAGAAGACCTAGAAATTGGTCCCAGTGTGTA GAATTAGCAAGATTAAAGTTTGAAAAATATTTTAACCATAAGGCTCTTCAGCTTCTTCACTGTTTCCCTC TGGACATACGATTAAAAGATGGCAGTTTATTTTGGCAGTCACCAAAGAGGCCACCCTCTCCAATAAAATT TGATTTAAATGAGCCTTTGCACCTCAGTTTCCTTCAGAATGCTGCAAAACTATATGCTACAGTATATTGT ATTCCATTTGCAGAAGAGGACTTATCAGCAGATGCCCTCTTGAATATTCTTTCAGAAGTAAAGATTCAGG AATTCAAGCCTTCCAATAAGGTTGTTCAAACAGATGAAACTGCAAGGAAACCAGACCATGTTCCTATTAG CAGTGAAGATGAGAGGAATGCAATTTTCCAACTAGAAAAGGCTATTTTATCTAATGAAGCCACCAAAAGT GACCTTCAGATGGCAGTGCTTTCATTTGAAAAAGATGATGATCATAATGGACACATAGATTTCATCACAG CTGCATCAAATCTTCGTGCCAAAATGTACAGCATTGAACCAGCTGACCGTTTCAAAACAAAGCGCATAGC TGGTAAAATTATACCTGCTATAGCAACAACCACTGCTACAGTTTCTGGCTTGGTTGCCTTGGAGATGATC AAAGTAACTGGTGGCTATCCATTTGAAGCTTACAAAAATTGTTTTCTTAACTTAGCCATTCCAATTGTAG TATTTACAGAGACAACTGAAGTAAGGAAAACTAAAATCAGAAATGGAATATCATTTACAATTTGGGATCG ATGGACCGTACATGGAAAAGAAGATTTCACCCTCTTGGATTTCATAAATGCAGTCAAAGAGAAGTATGGA ATTGAGCCAACAATGGTGGTACAGGGAGTCAAAATGCTTTATGTTCCTGTAATGCCTGGTCATGCAAAAA GATTGAAGTTAACAATGCATAAACTTGTAAAACCTACTACTGAAAAGAAATATGTGGATCTTACTGTGTC ATTTGCTCCAGACATTGATGGAGATGAAGATTTGCCGGGACCTCCAGTAAGATACTACTTCAGTCATGAC ACTGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC214483 representing NM_018227
Red=Cloning site Green=Tags(s) MEGSEPVAAHQGEEASCSSWGTGSTNKNLPIMSTASVEIDDALYSRQRYVLGDTAMQKMAKSHVFLSGMG GLGLEIAKNLVLAGIKAVTIHDTEKCQAWDLGTNFFLSEDDVVNKRNRAEAVLKHIAELNPYVHVTSSSV PFNETTDLSFLDKYQCVVLTEMKLPLQKKINDFCRSQCPPIKFISADVHGIWSRLFCDFGDEFEVLDTTG EEPKEIFISNITQANPGIVTCLENHPHKLETGQFLTFREINGMTGLNGSIQQITVISPFSFSIGDTTELE PYLHGGIAVQVKTPKTVFFESLERQLKHPKCLIVDFSNPEAPLEIHTAMLALDQFQEKYSRKPNVGCQQD SEELLKLATSISETLEEKPDVNADIVHWLSWTAQGFLSPLAAAVGGVASQEVLKAVTGKFSPLCQWLYLE AADIVESLGKPECEEFLPRGDRYDALRACIGDTLCQKLQNLNIFLVGCGAIGCEMLKNFALLGVGTSKEK GMITVTDPDLIEKSNLNRQFLFRPHHIQKPKSYTAADATLKINSQIKIDAHLNKVCPTTETIYNDEFYTK QDVIITALDNVEARRYVDSRCLANLRPLLDSGTMGTKGHTEVIVPHLTESYNSHRDPPEEEIPFCTLKSF PAAIEHTIQWARDKFESSFSHKPSLFNKFWQTYSSAEEVLQKIQSGHSLEGCFQVIKLLSRRPRNWSQCV ELARLKFEKYFNHKALQLLHCFPLDIRLKDGSLFWQSPKRPPSPIKFDLNEPLHLSFLQNAAKLYATVYC IPFAEEDLSADALLNILSEVKIQEFKPSNKVVQTDETARKPDHVPISSEDERNAIFQLEKAILSNEATKS DLQMAVLSFEKDDDHNGHIDFITAASNLRAKMYSIEPADRFKTKRIAGKIIPAIATTTATVSGLVALEMI KVTGGYPFEAYKNCFLNLAIPIVVFTETTEVRKTKIRNGISFTIWDRWTVHGKEDFTLLDFINAVKEKYG IEPTMVVQGVKMLYVPVMPGHAKRLKLTMHKLVKPTTEKKYVDLTVSFAPDIDGDEDLPGPPVRYYFSHD TD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018227 |
| ORF Size | 3156 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018227.6 |
| RefSeq Size | 6462 bp |
| RefSeq ORF | 3159 bp |
| Locus ID | 55236 |
| UniProt ID | A0AVT1 |
| Domains | UBACT, ThiF |
| Protein Pathways | Ubiquitin mediated proteolysis |
| MW | 117.8 kDa |
| Gene Summary | Modification of proteins with ubiquitin (UBB; MIM 191339) or ubiquitin-like proteins controls many signaling networks and requires a ubiquitin-activating enzyme (E1), a ubiquitin conjugating enzyme (E2), and a ubiquitin protein ligase (E3). UBE1L2 is an E1 enzyme that initiates the activation and conjugation of ubiquitin-like proteins (Jin et al., 2007 [PubMed 17597759]).[supplied by OMIM, Mar 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214483L1 | Lenti ORF clone of Human ubiquitin-like modifier activating enzyme 6 (UBA6), Myc-DDK-tagged |
CNY 9,728.00 |
|
| RC214483L2 | Lenti ORF clone of Human ubiquitin-like modifier activating enzyme 6 (UBA6), mGFP tagged |
CNY 10,260.00 |
|
| RC214483L3 | Lenti ORF clone of Human ubiquitin-like modifier activating enzyme 6 (UBA6), Myc-DDK-tagged |
CNY 10,260.00 |
|
| RC214483L4 | Lenti ORF clone of Human ubiquitin-like modifier activating enzyme 6 (UBA6), mGFP tagged |
CNY 10,260.00 |
|
| RG214483 | UBA6 (tGFP-tagged) - Human ubiquitin-like modifier activating enzyme 6 (UBA6) |
CNY 8,928.00 |
|
| SC111323 | UBA6 (untagged)-Human ubiquitin-like modifier activating enzyme 6 (UBA6) |
CNY 14,224.00 |
|
| SC318471 | UBA6 (untagged)-Human ubiquitin-like modifier activating enzyme 6 (UBA6) |
CNY 7,336.00 |
