United States
Germany
Japan
United Kingdom
China
CHRNA1 (NM_000079) Human Tagged ORF Clone
CAT＃: RC216321
CHRNA1 (Myc-DDK-tagged)-Human cholinergic receptor, nicotinic, alpha 1 (muscle) (CHRNA1), transcript variant 2
ORF Plasmid: tGFP
"NM_000079" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ACHRA; ACHRD; CHRNA; CMS1A; CMS1B; CMS2A; FCCMS; SCCMS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC216321 representing NM_000079
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCCTGGCCTCTCCTCCTGCTCTTTAGCCTTTGCTCAGCTGGCCTCGTCCTGGGCTCCGAACATG AGACCCGTCTGGTGGCAAAGCTATTTAAAGACTACAGCAGCGTGGTGCGGCCAGTGGAAGACCACCGCCA GGTCGTGGAGGTCACCGTGGGCCTGCAGCTGATACAGCTCATCAATGTGGATGAAGTAAATCAGATCGTG ACAACCAATGTGCGTCTGAAACAGCAATGGGTGGATTACAACCTAAAATGGAATCCAGATGACTATGGCG GTGTGAAAAAAATTCACATTCCTTCAGAAAAGATCTGGCGCCCAGACCTTGTTCTCTATAACAATGCAGA TGGTGACTTTGCTATTGTCAAGTTCACCAAAGTGCTCCTGCAGTACACTGGCCACATCACGTGGACACCT CCAGCCATCTTTAAAAGCTACTGTGAGATCATCGTCACCCACTTTCCCTTTGATGAACAGAACTGCAGCA TGAAGCTGGGCACCTGGACCTACGACGGCTCTGTCGTGGCCATCAACCCGGAAAGCGACCAGCCAGACCT GAGCAACTTCATGGAGAGCGGGGAGTGGGTGATCAAGGAGTCCCGGGGCTGGAAGCACTCCGTGACCTAT TCCTGCTGCCCCGACACCCCCTACCTGGACATCACCTACCACTTCGTCATGCAGCGCCTGCCCCTCTACT TCATCGTCAACGTCATCATCCCCTGCCTGCTCTTCTCCTTCTTAACTGGCCTGGTATTCTACCTGCCCAC AGACTCAGGGGAGAAGATGACTCTGAGCATCTCTGTCTTACTGTCTTTGACTGTGTTCCTTCTGGTCATC GTGGAGCTGATCCCCTCCACGTCCAGTGCTGTGCCCTTGATTGGAAAATACATGCTGTTCACCATGGTGT TCGTCATTGCCTCCATCATCATCACTGTCATCGTCATCAACACACACCACCGCTCACCCAGCACCCATGT CATGCCCAACTGGGTGCGGAAGGTTTTTATCGACACTATCCCAAATATCATGTTTTTCTCCACAATGAAA AGACCATCCAGAGAAAAGCAAGACAAAAAGATTTTTACAGAAGACATTGATATCTCTGACATTTCTGGAA AGCCAGGGCCTCCACCCATGGGCTTCCACTCTCCCCTGATCAAACACCCCGAGGTGAAAAGTGCCATCGA GGGCATCAAGTACATCGCAGAGACCATGAAGTCAGACCAGGAGTCTAACAATGCGGCGGCAGAGTGGAAG TACGTTGCAATGGTGATGGACCACATACTCCTCGGAGTCTTCATGCTTGTTTGCATCATCGGAACCCTAG CCGTGTTTGCAGGTCGACTCATTGAATTAAATCAGCAAGGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC216321 representing NM_000079
Red=Cloning site Green=Tags(s) MEPWPLLLLFSLCSAGLVLGSEHETRLVAKLFKDYSSVVRPVEDHRQVVEVTVGLQLIQLINVDEVNQIV TTNVRLKQQWVDYNLKWNPDDYGGVKKIHIPSEKIWRPDLVLYNNADGDFAIVKFTKVLLQYTGHITWTP PAIFKSYCEIIVTHFPFDEQNCSMKLGTWTYDGSVVAINPESDQPDLSNFMESGEWVIKESRGWKHSVTY SCCPDTPYLDITYHFVMQRLPLYFIVNVIIPCLLFSFLTGLVFYLPTDSGEKMTLSISVLLSLTVFLLVI VELIPSTSSAVPLIGKYMLFTMVFVIASIIITVIVINTHHRSPSTHVMPNWVRKVFIDTIPNIMFFSTMK RPSREKQDKKIFTEDIDISDISGKPGPPPMGFHSPLIKHPEVKSAIEGIKYIAETMKSDQESNNAAAEWK YVAMVMDHILLGVFMLVCIIGTLAVFAGRLIELNQQG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000079 |
| ORF Size | 1371 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000079.4 |
| RefSeq Size | 1667 bp |
| RefSeq ORF | 1374 bp |
| Locus ID | 1134 |
| UniProt ID | P02708 |
| Protein Families | Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane |
| MW | 51.84 kDa |
| Gene Summary | The muscle acetylcholine receptor consiststs of 5 subunits of 4 different types: 2 alpha subunits and 1 each of the beta, gamma, and delta subunits. This gene encodes an alpha subunit that plays a role in acetlycholine binding/channel gating. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Nov 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216321L1 | Lenti-ORF clone of CHRNA1 (Myc-DDK-tagged)-Human cholinergic receptor, nicotinic, alpha 1 (muscle) (CHRNA1), transcript variant 2 |
CNY 7,888.00 |
|
| RC216321L2 | Lenti-ORF clone of CHRNA1 (mGFP-tagged)-Human cholinergic receptor, nicotinic, alpha 1 (muscle) (CHRNA1), transcript variant 2 |
CNY 5,990.00 |
|
| RC216321L3 | Lenti-ORF clone of CHRNA1 (Myc-DDK-tagged)-Human cholinergic receptor, nicotinic, alpha 1 (muscle) (CHRNA1), transcript variant 2 |
CNY 5,990.00 |
|
| RC216321L4 | Lenti-ORF clone of CHRNA1 (mGFP-tagged)-Human cholinergic receptor, nicotinic, alpha 1 (muscle) (CHRNA1), transcript variant 2 |
CNY 5,990.00 |
|
| RG216321 | CHRNA1 (tGFP-tagged) - Human cholinergic receptor, nicotinic, alpha 1 (muscle) (CHRNA1), transcript variant 2 |
CNY 4,470.00 |
|
| SC310367 | CHRNA1 (untagged)-Human cholinergic receptor, nicotinic, alpha 1 (muscle) (CHRNA1), transcript variant 2 |
CNY 5,856.00 |
