CHST13 (NM_152889) Human Tagged ORF Clone

CAT#: RC216985

CHST13 (Myc-DDK-tagged)-Human carbohydrate (chondroitin 4) sulfotransferase 13 (CHST13)

ORF Plasmid: DDK tGFP



  "NM_152889" in other vectors (4)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit polyclonal anti-CHST13 antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms C4ST3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC216985 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGAGGCGCTGCTGCCGGCGGCGCGTGCTGGCGGCCGCCTGTCTGGGCGCCGCGCTCCTGCTCCTAT
GCGCCGCGCCCCGCTCCCTGCGCCCGGCATTTGGAAACAGAGCCCTGGGCTCCAGCTGGCTTGGTGGGGA
GAAGAGAAGCCCCCTGCAGAAGCTCTATGACCTGGATCAGGACCCGCGCTCGACCCTGGCGAAGGTGCAC
CGTCAGCGGCGCGACCTGCTGAACAGCGCCTGTAGCCGCCACTCACGCCGGCAGCGCCTGCTACAGCCGG
AGGACCTGCGGCACGTGCTGGTGGACGACGCGCATGGCCTGCTCTACTGCTACGTGCCCAAGGTGGCCTG
CACCAACTGGAAGCGCGTGCTGCTGGCGCTGAGCGGCCAAGCCCGCGGCGACCCGCGCGCCATCTCCGCG
CAAGAGGCGCACGCGCCTGGCCGCCTGCCCTCACTGGCCGACTTCAGCCCCGCCGAGATCAACCGGCGCC
TGCGCGCCTACTTGGCCTTCCTGTTCGTGCGGGAGCCCTTCGAGCGCCTGGCATCGGCTTACCGCAACAA
GCTCGCGCGCCCCTACAGCGCCGCCTTCCAGAGGCGCTACGGTGCACGCATCGTTCAGCGCCTGCGGCCG
CGCGCGCTCCCCGACGCCCGGGCCCGCGGCCACGACGTGCGCTTCGCGGAGTTCCTGGCCTACCTGCTGG
ACCCGCGCACGCGGCGTGAGGAGCCCTTCAACGAGCACTGGGAGCGCGCGCACGCGCTCTGCCACCCGTG
TCGCCTCCGCTACGACGTCGTGGGCAAGTTCGAGACACTGGTGGAGGACGCGGCCTTCGTGCTGGGCCTG
GCGGGCGCATCCGACCTGAGCTTCCCTGGGCCGCCGCGGCCCCGGGGAGCCGCCGCCTCCCGCGACCTGG
CAGCGCGCCTCTTCCGGGACATCAGCCCCTTCTACCAGCAGCGCCTCTTCGACCTCTACAAGATGGACTT
CCTGCTTTTCAACTACTCCGCCCCCTCCTACCTGCGGCTGCTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC216985 protein sequence
Red=Cloning site Green=Tags(s)

MGRRCCRRRVLAAACLGAALLLLCAAPRSLRPAFGNRALGSSWLGGEKRSPLQKLYDLDQDPRSTLAKVH
RQRRDLLNSACSRHSRRQRLLQPEDLRHVLVDDAHGLLYCYVPKVACTNWKRVLLALSGQARGDPRAISA
QEAHAPGRLPSLADFSPAEINRRLRAYLAFLFVREPFERLASAYRNKLARPYSAAFQRRYGARIVQRLRP
RALPDARARGHDVRFAEFLAYLLDPRTRREEPFNEHWERAHALCHPCRLRYDVVGKFETLVEDAAFVLGL
AGASDLSFPGPPRPRGAAASRDLAARLFRDISPFYQQRLFDLYKMDFLLFNYSAPSYLRLL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_152889
ORF Size 1023 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_152889.3
RefSeq Size 1792 bp
RefSeq ORF 1026 bp
Locus ID 166012
UniProt ID Q8NET6
Domains Sulfotransfer2
Protein Families Transmembrane
Protein Pathways Chondroitin sulfate biosynthesis, Sulfur metabolism
MW 38.9 kDa
Gene Summary The protein encoded by this gene belongs to the sulfotransferase 2 family. It is localized to the golgi membrane, and catalyzes the transfer of sulfate to the C4 hydroxyl of beta-1,4-linked N-acetylgalactosamine (GalNAc) flanked by glucuronic acid residue in chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. [provided by RefSeq, Aug 2011]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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