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AMPD1 (NM_000036) Human Tagged ORF Clone
CAT＃: RC217919
AMPD1 (Myc-DDK-tagged)-Human adenosine monophosphate deaminase 1 (AMPD1), transcript variant 1
ORF Plasmid: tGFP
"NM_000036" in other vectors (6)
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CNY 5,472.00
CNY 8,270.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MAD; MADA; MMDD |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC217919 representing NM_000036
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTCTGTTCAAACTCCCAGCTGAAGAGAAACAAATTGATGATGCAATGCGCAACTTTGCTGAAAAAG TGTTTGCCTCTGAAGTCAAAGATGAAGGAGGTCGTCAGGAGATTTCCCCCTTTGATGTGGATGAGATCTG TCCGATTTCTCATCATGAGATGCAAGCACACATATTCCATCTGGAGACTCTGTCCACCTCCACAGAAGCC AGGAGAAAAAAGCGTTTCCAAGGACGGAAGACTGTTAATTTGTCCATTCCACTAAGTGAAACATCTTCCA CCAAACTGTCCCACATTGATGAATACATTTCCTCATCTCCAACCTACCAGACCGTGCCTGATTTTCAGAG AGTGCAGATTACTGGTGACTATGCCTCTGGGGTTACAGTTGAAGATTTTGAAATTGTTTGCAAAGGTCTG TATCGGGCACTATGCATACGTGAGAAATACATGCAGAAGTCGTTTCAGAGGTTCCCTAAAACCCCTTCCA AATACTTGCGGAACATTGATGGTGAGGCTTGGGTAGCAAATGAGAGCTTCTATCCAGTCTTTACTCCTCC TGTGAAGAAGGGAGAGGACCCCTTCCGAACAGACAACCTTCCTGAAAACCTGGGCTATCACCTCAAAATG AAGGACGGTGTAGTTTACGTCTATCCTAATGAAGCAGCAGTCAGCAAAGATGAGCCTAAGCCACTTCCTT ACCCAAATCTGGACACCTTCTTAGACGATATGAATTTTTTACTTGCTTTAATTGCTCAAGGACCTGTTAA GACCTATACCCACCGGCGCCTGAAGTTCCTCTCCTCCAAGTTCCAGGTCCATCAGATGCTTAACGAGATG GACGAGTTAAAGGAGCTGATAAACAACCCCCACCGAGATTTTTATAACTGCAGGAAGGTGGACACCCATA TCCATGCAGCCGCTTGCATGAACCAGAAACATCTGCTGCGTTTTATTAAGAAATCTTACCAAATTGATGC TGACAGAGTGGTCTATAGCACCAAAGAGAAGAATCTGACCCTAAAGGAACTTTTTGCTAAATTAAAAATG CATCCTTATGACCTGACTGTTGATTCTCTGGATGTTCATGCTGGACGCCAGACCTTCCAGCGTTTTGATA AGTTCAATGACAAATATAATCCTGTAGGAGCAAGTGAGCTACGGGACCTCTACTTGAAGACAGACAATTA CATTAATGGGGAATATTTTGCCACTATCATCAAGGAGGTAGGTGCGGACCTGGTGGAGGCCAAGTACCAG CATGCTGAGCCCCGCCTGTCCATCTATGGCCGCAGTCCTGATGAGTGGAGCAAACTCTCCTCCTGGTTCG TCTGCAATCGCATCCACTGCCCCAACATGACATGGATGATCCAGGTTCCCAGGATCTATGATGTGTTCCG TTCCAAGAATTTCCTTCCACATTTTGGAAAAATGCTGGAGAATATTTTCATGCCAGTGTTTGAGGCCACC ATCAACCCCCAGGCTGACCCAGAACTCAGTGTCTTCCTCAAGCATATCACTGGCTTTGACAGTGTGGATG ATGAGTCCAAACACAGTGGCCACATGTTCTCCTCCAAGAGTCCCAAGCCCCAGGAGTGGACATTGGAAAA GAATCCATCTTACACTTACTATGCCTACTACATGTATGCAAACATCATGGTGCTCAACAGCCTGAGAAAG GAACGAGGCATGAATACGTTTCTGTTCCGACCTCACTGTGGAGAAGCTGGAGCCCTCACCCATCTCATGA CAGCATTCATGATAGCAGATGATATCTCTCATGGCCTAAATTTAAAAAAGAGTCCCGTGCTACAGTACTT GTTTTTCTTAGCCCAAATTCCCATCGCCATGTCACCACTAAGTAACAATAGCCTATTTCTAGAGTATGCC AAAAATCCTTTTTTGGATTTCCTTCAGAAAGGGCTAATGATCTCACTGTCTACAGATGACCCAATGCAAT TCCACTTTACCAAGGAGCCCCTAATGGAAGAATATGCTATTGCTGCACAAGTCTTCAAGCTGAGCACCTG TGATATGTGCGAAGTGGCAAGGAACAGTGTCTTGCAGTGTGGAATTTCTCATGAGGAGAAAGTAAAGTTT CTGGGCGACAATTACCTTGAGGAAGGCCCTGCTGGAAATGATATCCGGAGGACAAATGTAGCCCAAATCC GCATGGCCTATCGCTATGAAACCTGGTGTTATGAACTCAATTTAATTGCTGAGGGTCTTAAATCAACAGA A ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC217919 representing NM_000036
Red=Cloning site Green=Tags(s) MPLFKLPAEEKQIDDAMRNFAEKVFASEVKDEGGRQEISPFDVDEICPISHHEMQAHIFHLETLSTSTEA RRKKRFQGRKTVNLSIPLSETSSTKLSHIDEYISSSPTYQTVPDFQRVQITGDYASGVTVEDFEIVCKGL YRALCIREKYMQKSFQRFPKTPSKYLRNIDGEAWVANESFYPVFTPPVKKGEDPFRTDNLPENLGYHLKM KDGVVYVYPNEAAVSKDEPKPLPYPNLDTFLDDMNFLLALIAQGPVKTYTHRRLKFLSSKFQVHQMLNEM DELKELINNPHRDFYNCRKVDTHIHAAACMNQKHLLRFIKKSYQIDADRVVYSTKEKNLTLKELFAKLKM HPYDLTVDSLDVHAGRQTFQRFDKFNDKYNPVGASELRDLYLKTDNYINGEYFATIIKEVGADLVEAKYQ HAEPRLSIYGRSPDEWSKLSSWFVCNRIHCPNMTWMIQVPRIYDVFRSKNFLPHFGKMLENIFMPVFEAT INPQADPELSVFLKHITGFDSVDDESKHSGHMFSSKSPKPQEWTLEKNPSYTYYAYYMYANIMVLNSLRK ERGMNTFLFRPHCGEAGALTHLMTAFMIADDISHGLNLKKSPVLQYLFFLAQIPIAMSPLSNNSLFLEYA KNPFLDFLQKGLMISLSTDDPMQFHFTKEPLMEEYAIAAQVFKLSTCDMCEVARNSVLQCGISHEEKVKF LGDNYLEEGPAGNDIRRTNVAQIRMAYRYETWCYELNLIAEGLKSTE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000036 |
| ORF Size | 2241 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000036.1, NP_000027.1 |
| RefSeq Size | 2426 bp |
| RefSeq ORF | 2244 bp |
| Locus ID | 270 |
| UniProt ID | P23109 |
| Domains | A_deaminase |
| Protein Families | Druggable Genome |
| Protein Pathways | Metabolic pathways, Purine metabolism |
| MW | 86.5 kDa |
| Gene Summary | Adenosine monophosphate deaminase 1 catalyzes the deamination of AMP to IMP in skeletal muscle and plays an important role in the purine nucleotide cycle. Two other genes have been identified, AMPD2 and AMPD3, for the liver- and erythocyte-specific isoforms, respectively. Deficiency of the muscle-specific enzyme is apparently a common cause of exercise-induced myopathy and probably the most common cause of metabolic myopathy in the human. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Feb 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217919L1 | Lenti ORF clone of Human adenosine monophosphate deaminase 1 (AMPD1), transcript variant 1, Myc-DDK-tagged |
CNY 7,872.00 |
|
| RC217919L2 | Lenti ORF clone of Human adenosine monophosphate deaminase 1 (AMPD1), transcript variant 1, mGFP tagged |
CNY 10,170.00 |
|
| RC217919L3 | Lenti ORF clone of Human adenosine monophosphate deaminase 1 (AMPD1), transcript variant 1, Myc-DDK-tagged |
CNY 10,170.00 |
|
| RC217919L4 | Lenti ORF clone of Human adenosine monophosphate deaminase 1 (AMPD1), transcript variant 1, mGFP tagged |
CNY 7,872.00 |
|
| RG217919 | AMPD1 (tGFP-tagged) - Human adenosine monophosphate deaminase 1 (AMPD1), transcript variant 1 |
CNY 7,072.00 |
|
| SC113553 | AMPD1 (untagged)-Human adenosine monophosphate deaminase 1 (AMPD1), transcript variant 1 |
CNY 5,480.00 |
