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DACH1 (NM_080760) Human Tagged ORF Clone
CAT＃: RC219215
- TrueORF®
DACH1 (Myc-DDK-tagged)-Human dachshund homolog 1 (Drosophila) (DACH1), transcript variant 2
ORF Plasmid: tGFP
"NM_080760" in other vectors (5)
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CNY 4,940.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DACH |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC219215 representing NM_080760
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGTGCCGGCGGCTTTGATCCCTCCGACCCAGCTGGTCCCCCCTCAACCCCCAATCTCCACGTCTG CTTCCTCCTCTGGCACCACCACCTCCACCTCTTCGGCGACTTCGTCTCCGGCTCCTTCCATCGGACCCCC GGCGTCCTCTGGGCCAACTCTGTTCCGCCCGGAGCCCATCGCTTCGGCGGCGGCGGCGGCGGCCACAGTC ACCTCTACCGGCGGCGGCGGCGGCGGCGGCGGCAGCGGAGGCGGCGGCGGCAGCAGCGGCAACGGAGGCG GCGGTGGCGGCGGCGGCGGTGGCAGCAACTGCAACCCCAACCTGGCGGCCGCGAGCAACGGCAGCGGCGG CGGCGGCGGCGGCATCAGCGCTGGCGGCGGCGTCGCTTCCAGCACCCCCATCAACGCCAGCACCGGCAGC AGCAGCAGCAGCAGTAGCAGCAGCAGCAGCAGCAGCAGTAGTAGCAGCAGCAGCAGTAGCAGCAGCAGCT GCGGCCCCCTCCCCGGGAAACCCGTGTACTCAACCCCGTCCCCAGTGGAAAACACCCCTCAGAATAATGA GTGCAAAATGGTGGATCTGAGGGGGGCCAAAGTGGCTTCCTTCACGGTGGAGGGCTGCGAGCTGATCTGC CTGCCCCAGGCTTTCGACCTGTTCCTGAAGCACTTGGTGGGGGGCTTGCATACGGTCTACACCAAGCTGA AGCGGCTGGAGATCACGCCGGTGGTGTGCAATGTGGAACAAGTTCGCATCCTGAGGGGACTGGGCGCCAT CCAGCCAGGAGTGAACCGCTGCAAACTCATCTCCAGGAAGGACTTCGAGACCCTCTACAATGACTGCACC AACGCAAGTTCTAGACCTGGAAGGCCTCCTAAGAGGACTCAAAGTGTCACCTCCCCAGAGAACTCTCACA TCATGCCGCATTCTGTCCCTGGTCTCATGTCTCCTGGGATAATTCCACCAACAGGTCTGACAGCAGCCGC TGCAGCAGCTGCTGCTGCTACCAATGCAGCTATTGCTGAAGCAATGAAGGTGAAAAAAATCAAATTAGAA GCCATGAGCAACTATCATGCCAGTAATAACCAACATGGAGCAGACTCTGAAAACGGGGACATGAATTCAA GTGTCGATGAGACCCCGCTTTCTACACCAACCGCAAGAGACAGCCTTGACAAACTCTCTCTAACTGGGCA TGGACAACCACTGCCTCCAGGTTTTCCATCTCCTTTTCTGTTTCCTGATGGACTGTCTTCCATCGAGACT CTTCTGACTAACATACAGGGGCTGTTGAAAGTTGCCATAGATAATGCCAGAGCTCAAGAGAAACAGGTCC AACTGGAAAAAACTGAGCTGAAGATGGATTTTTTAAGGGAAAGAGAACTAAGGGAAACACTTGAGAAGCA GTTGGCTATGGAACAAAAGAATAGAGCCATAGTTCAAAAGAGGCTAAAGAAGGAGAAGAAGGCAAAGAGA AAATTGCAGGAAGCACTTGAGTTTGAGACGAAACGGCGTGAACAAGCAGAACAGACGCTAAAACAGGCAG CTTCAACAGATAGTCTCAGGGTCTTAAATGACTCTCTGACCCCAGAGATAGAGGCTGACCGCAGTGGCGG CAGAACAGATGCTGAAAGGACAATACAAGATGGAAGACTGTATTTGAAAACTACTGTCATGTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219215 representing NM_080760
Red=Cloning site Green=Tags(s) MAVPAALIPPTQLVPPQPPISTSASSSGTTTSTSSATSSPAPSIGPPASSGPTLFRPEPIASAAAAAATV TSTGGGGGGGGSGGGGGSSGNGGGGGGGGGGSNCNPNLAAASNGSGGGGGGISAGGGVASSTPINASTGS SSSSSSSSSSSSSSSSSSSSSSSCGPLPGKPVYSTPSPVENTPQNNECKMVDLRGAKVASFTVEGCELIC LPQAFDLFLKHLVGGLHTVYTKLKRLEITPVVCNVEQVRILRGLGAIQPGVNRCKLISRKDFETLYNDCT NASSRPGRPPKRTQSVTSPENSHIMPHSVPGLMSPGIIPPTGLTAAAAAAAAATNAAIAEAMKVKKIKLE AMSNYHASNNQHGADSENGDMNSSVDETPLSTPTARDSLDKLSLTGHGQPLPPGFPSPFLFPDGLSSIET LLTNIQGLLKVAIDNARAQEKQVQLEKTELKMDFLRERELRETLEKQLAMEQKNRAIVQKRLKKEKKAKR KLQEALEFETKRREQAEQTLKQAASTDSLRVLNDSLTPEIEADRSGGRTDAERTIQDGRLYLKTTVMY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_080760 |
| ORF Size | 1674 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_080760.5, NP_542938.3 |
| RefSeq Size | 4796 bp |
| RefSeq ORF | 1677 bp |
| Locus ID | 1602 |
| UniProt ID | Q9UI36 |
| Domains | Ski_Sno |
| Protein Families | Transcription Factors |
| MW | 57.3 kDa |
| Gene Summary | This gene encodes a chromatin-associated protein that associates with other DNA-binding transcription factors to regulate gene expression and cell fate determination during development. The protein contains a Ski domain that is highly conserved from Drosophila to human. Expression of this gene is lost in some forms of metastatic cancer, and is correlated with poor prognosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219215L3 | Lenti-ORF clone of DACH1 (Myc-DDK-tagged)-Human dachshund homolog 1 (Drosophila) (DACH1), transcript variant 2 |
CNY 6,840.00 |
|
| RC219215L4 | Lenti-ORF clone of DACH1 (mGFP-tagged)-Human dachshund homolog 1 (Drosophila) (DACH1), transcript variant 2 |
CNY 6,840.00 |
|
| RG219215 | DACH1 (tGFP-tagged) - Human dachshund homolog 1 (Drosophila) (DACH1), transcript variant 2 |
CNY 8,248.00 |
|
| SC313997 | DACH1 (untagged)-Human dachshund homolog 1 (Drosophila) (DACH1), transcript variant 2 |
CNY 4,160.00 |
|
| SC327921 | DACH1 (untagged)-Human dachshund homolog 1 (Drosophila) (DACH1) transcript variant 2 |
CNY 9,030.00 |
