MAST4 (NM_015183) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC219460 representing NM_015183 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGAGTCCAGCATTCTAAGACGAAGAGGGCTCCAGAAGGAGCTGAGTCTCCCCAGAAGAGGAAGTT TGATAGATTCCCAGAAGTGGAATTGCTTGGTCAAACGTTGCCGAACAAGCAACCGGAAAAGCTTAATAGG CAATGGGCAGTCACCAGCATTGCCTCGACCACACTCACCTCTCTCTGCTCATGCAGGAAATAGCCCTCAA GATAGTCCAAGAAATTTCTCCCCCAGTGCCTCAGCCCATTTTTCATTTGCACGGAGGACTGATGGACGCC GCTGGTCGTTGGCTTCTCTCCCTTCCTCTGGCTATGGGACAAACACACCCAGCTCTACGGTCTCTTCATC CTGTTCCTCCCAGGAGAAGTTGCATCAGTTACCATACCAACCAACACCAGACGAGTTACACTTCTTATCA AAACATTTCTGTACCACCGAAAGCATCGCCACTGAGAACAGATGCAGGAACACGCCGATGCGCCCCCGTT CCCGAAGTCTGAGCCCTGGACGTTCTCCCGCCTGCTGTGACCATGAAATAATTATGATGAACCATGTCTA CAAAGAAAGGTTCCCAAAGGCTACAGCTCAGATGGAAGAACGTCTAAAGGAAATTATCACCAGCTACTCT CCTGACAACGTTCTACCCTTAGCAGATGGAGTGCTTAGTTTCACTCACCACCAGATTATTGAACTGGCTC GAGATTGCTTGGATAAATCCCACCAGGGCCTCATCACCTCACGATACTTCCTTGAATTACAGCACAAATT AGATAAGTTGCTACAGGAGGCTCATGATCGTTCAGAAAGTGGAGAATTGGCATTTATTAAACAACTAGTT CGAAAGATCCTAATTGTTATTGCCCGCCCTGCTCGGTTATTAGAGTGCCTGGAATTTGATCCGGAAGAAT TTTACTACCTATTGGAAGCAGCAGAAGGCCATGCCAAAGAAGGACAGGGTATTAAAACCGACATTCCCAG GTACATCATTAGCCAACTGGGACTCAATAAGGATCCCTTGGAAGAAATGGCTCATTTGGGAAACTACGAT AGTGGGACAGCAGAAACACCAGAAACAGATGAATCAGTGAGTAGCTCTAATGCCTCCCTGAAACTTCGAA GGAAACCTCGGGAAAGTGATTTTGAAACGATTAAATTGATTAGCAATGGAGCCTATGGGGCAGTCTACTT TGTTCGGCATAAAGAATCCCGGCAGAGGTTTGCCATGAAGAAGATTAATAAACAGAACCTCATCCTTCGA AACCAGATCCAGCAGGCCTTTGTGGAGCGGGATATCCTGACTTTTGCAGAAAACCCCTTTGTTGTCAGCA TGTATTGCTCCTTTGAAACAAGGCGCCACTTGTGCATGGTCATGGAATATGTGGAAGGGGGAGACTGTGC TACTTTAATGAAAAACATGGGTCCTCTCCCTGTTGATATGGCCAGAATGTACTTTGCTGAGACGGTCTTG GCCTTGGAATATTTACATAATTATGGAATTGTACACAGGGATTTGAAACCAGACAACTTGTTGGTTACCT CCATGGGGCACATAAAGCTGACAGATTTTGGATTATCTAAGGTGGGACTAATGAGCATGACTACCAACCT TTACGAGGGTCATATTGAGAAGGATGCTAGAGAGTTCCTGGATAAACAGGTCTGTGGCACACCTGAATAC ATTGCACCAGAAGTGATTCTGAGGCAGGGTTATGGAAAGCCGGTGGACTGGTGGGCCATGGGGATTATCC TCTATGAATTTCTGGTTGGATGCGTGCCATTCTTTGGGGATACTCCAGAGGAGCTATTTGGACAAGTCAT CAGTGATGAGATCAACTGGCCTGAGAAGGATGAGGCACCCCCACCTGATGCCCAGGATCTGATTACCTTA CTCCTCAGGCAGAATCCCCTGGAGAGGCTGGGAACAGGTGGTGCATATGAAGTCAAACAGCATCGATTCT TCCGTTCTTTAGACTGGAACAGTTTGCTGAGACAGAAGGCAGAATTTATTCCCCAACTGGAATCTGAGGA TGACACAAGTTATTTTGATACTCGGTCTGAGAAGTATCATCATATGGAAACGGAGGAAGAAGATGACACA AATGATGAAGACTTTAATGTGGAAATAAGGCAGTTTTCTTCATGTTCACACAGGTTTTCAAAAGTTTTCA GCAGTATAGATCGAATCACTCAGAATTCAGCAGAAGAGAAGGAAGACTCTGTGGACAAAACCAAAAGCAC CACCTTGCCATCCACAGAAACACTGAGCTGGAGTTCAGAATATTCTGAAATGCAACAGCTATCAACATCC AACTCTTCAGATACTGAAAGCAACAGACATAAACTCAGTTCTGGCCTACTTCCCAAACTGGCTATTTCAA CAGAGGGAGAGCAAGATGAAGCTGCCTCCTGCCCTGGAGACCCCCATGAGGAGCCAGGAAAGCCAGCCCT TCCTCCTGAAGAGTGTGCCCAGGAGGAGCCTGAGGTCACCACCCCAGCCAGCACCATCAGCAGCTCCACC CTGTCAGTTGGCAGTTTTTCAGAGCACTTGGATCAGATAAATGGACGAAGCGAGTGTGTGGACAGTACAG ATAATTCCTCAAAGCCATCCAGTGAACCCGCTTCTCACATGGCTCGGCAGCGATTAGAAAGCACAGAAAA AAAGAAAATCTCGGGGAAAGTCACAAAGTCCCTCTCTGCCAGTGCTCTTTCCCTCATGATCCCAGGAGAT ATGTTTGCTGTTTCCCCTCTGGGAAGTCCAATGTCTCCCCATTCCCTGTCCTCGGACCCTTCTTCTTCAC GAGATTCCTCTCCCAGCCGAGATTCCTCAGCAGCTTCTGCCAGTCCACATCAGCCGATTGTGATCCACAG TTCGGGGAAGAACTACGGCTTTACCATCCGAGCCATCCGGGTGTATGTGGGAGACAGTGACATCTATACA GTGCACCATATCGTCTGGAATGTAGAAGAAGGAAGTCCGGCATGCCAGGCAGGACTGAAGGCTGGAGATC TTATCACTCACATCAATGGAGAACCAGTGCATGGACTTGTCCACACAGAAGTTATAGAACTCCTACTGAA GAGTGGGAATAAGGTGTCAATCACTACTACCCCATTTGAAAACACATCAATCAAAACTGGACCAGCCAGG AGAAACAGCTATAAGAGCCGGATGGTGAGGCGGAGCAAGAAATCCAAGAAGAAAGAAAGTCTCGAAAGGA GGAGATCTCTTTTCAAAAAGCTAGCCAAGCAGCCTTCTCCTTTACTCCACACCAGCCGAAGTTTCTCCTG CTTGAACAGATCCCTGTCATCGGGTGAGAGCCTCCCAGGTTCCCCCACTCATAGCTTGTCTCCCCGGTCT CCAACACCAAGCTACCGCTCCACCCCTGACTTCCCATCTGGTACTAATTCCTCCCAGAGCAGCTCCCCTA GTTCTAGTGCCCCCAATTCCCCAGCAGGGTCCGGGCACATCCGGCCCAGCACTCTCCACGGTCTTGCACC CAAACTCGGCGGGCAGCGGTACCGGTCCGGAAGGCGAAAGTCCGCCGGCAACATCCCACTGTCCCCGCTG GCCCGGACGCCCTCTCCAACCCCGCAACCCACCTCCCCGCAGCGGTCACCATCCCCTCTTCTGGGACACT CACTGGGCAATTCCAAGATCGCGCAAGCCTTTCCCAGCAAGATGCACTCCCCGCCCACCATCGTCAGACA CATCGTGAGGCCCAAGAGTGCGGAGCCCCCCAGGTCCCCGCTGCTCAAGCGCGTGCAGTCCGAGGAGAAG CTGTCGCCCTCTTACGGCAGTGACAAGAAGCACCTGTGCTCCCGCAAGCACAGCCTGGAGGTGACCCAAG AGGAGGTGCAGCGGGAGCAGTCCCAGCGGGAGGCGCCGCTGCAGAGCCTGGATGAGAACGTGTGCGACGT GCCGCCGCTCAGCCGCGCCCGGCCAGTGGAGCAAGGCTGCCTGAAACGCCCAGTCTCCCGGAAGGTGGGC CGCCAGGAGTCTGTGGACGACCTGGACCGCGACAAGCTGAAGGCCAAGGTGGTGGTGAAGAAAGCAGACG GCTTCCCAGAGAAACAGGAATCCCACCAGAAATCCCATGGACCCGGGAGTGATTTGGAAAACTTTGCTCT GTTTAAGCTGGAAGAGAGAGAGAAGAAAGTCTATCCGAAGGCTGTGGAAAGGTCAAGTACTTTTGAAAAC AAAGCGTCTATGCAGGAGGCGCCACCGCTGGGCAGCCTGCTGAAGGATGCTCTTCACAAGCAGGCCAGCG TGCGCGCCAGCGAGGGTGCGATGTCGGATGGCCGGGTGCCTGCGGAGCACCGCCAGGGTGGCGGGGACTT CAGACGGGCCCCCGCTCCTGGCACCCTCCAGGATGGTCTCTGCCACTCCCTCGACAGGGGCATCTCTGGG AAGGGGGAAGGCACGGAGAAGTCCTCCCAGGCCAAGGAGCTTCTCCGATGTGAAAAGTTAGACAGCAAGC TGGCCAACATCGATTACCTCCGAAAGAAAATGTCACTTGAGGACAAAGAGGACAACCTCTGCCCTGTGCT GAAGCCCAAGATGACAGCTGGCTCCCACGAATGCCTGCCAGGGAACCCAGTCCGACCCACGGGTGGGCAG CAGGAGCCCCCGCCGGCTTCTGAGAGCCGAGCTTTTGTCAGCAGCACCCATGCAGCTCAGATGAGTGCCG TCTCTTTTGTTCCCCTCAAGGCCTTAACAGGCCGGGTGGACAGTGGAACGGAGAAGCCTGGCTTGGTTGC TCCTGAGTCCCCTGTTAGGAAGAGCCCCTCCGAGTATAAGCTGGAAGGTAGGTCTGTCTCATGCCTGAAG CCGATCGAGGGCACTCTGGACATTGCTCTCCTGTCCGGACCTCAGGCCTCCAAGACAGAACTGCCTTCCC CAGAGTCTGCACAGAGCCCCAGCCCAAGTGGTGACGTGAGGGCCTCTGTGCCACCAGTTCTCCCCAGCAG CAGTGGGAAAAAGAACGATACCACCAGTGCAAGAGAGCTTTCTCCTTCCAGCTTAAAGATGAATAAATCC TACCTGCTGGAGCCTTGGTTCCTGCCCCCCAGCCGAGGTCTCCAGAATTCACCAGCAGTTTCCCTGCCTG ACCCAGAGTTCAAGAGGGACAGGAAAGGTCCCCATCCTACTGCCAGGAGCCCTGGAACAGTCATGGAAAG CAATCCCCAACAGAGAGAGGGCAGCTCCCCTAAACACCAAGACCACACCACTGACCCCAAGCTTCTGACC TGCCTGGGGCAGAACCTCCACAGCCCTGACCTGGCCAGGCCACGCTGCCCGCTCCCACCTGAAGCTTCCC CCTCAAGGGAGAAGCCAGGCCTGAGGGAATCGTCTGAAAGAGGCCCTCCCACAGCCAGAAGCGAGCGCTC TGCTGCGAGGGCTGACACATGCAGAGAGCCCTCCATGGAACTGTGCTTTCCAGAAACTGCGAAAACCAGT GACAACTCCAAAAATCTCCTCTCTGTGGGAAGGACCCACCCAGATTTCTATACACAGACCCAGGCCATGG AGAAAGCATGGGCGCCGGGTGGGAAAACGAACCACAAAGATGGCCCAGGTGAGGCGAGGCCCCCGCCCAG AGACAACTCCTCTCTGCACTCAGCTGGAATTCCCTGTGAGAAGGAGCTGGGCAAGGTGAGGCGTGGCGTG GAACCCAAGCCCGAAGCGCTTCTTGCCAGGCGGTCTCTGCAGCCACCTGGAATTGAGAGTGAGAAGAGTG AAAAGCTCTCCAGTTTCCCATCTTTGCAGAAAGATGGTGCCAAGGAACCTGAAAGGAAGGAGCAGCCTCT ACAAAGGCATCCCAGCAGCATCCCTCCGCCCCCTCTGACGGCCAAAGACCTGTCCAGCCCGGCTGCCAGG CAGCATTGCAGTTCCCCAAGCCACGCTTCTGGCAGAGAGCCGGGGGCCAAGCCCAGCACTGCAGAGCCCA GCTCGAGCCCCCAGGACCCTCCCAAGCCTGTTGCTGCGCACAGTGAAAGCAGCAGCCACAAGCCCCGGCC TGGCCCTGACCCGGGCCCTCCAAAGACTAAGCACCCCGACCGGTCCCTCTCCTCTCAGAAACCAAGTGTC GGGGCCACAAAGGGCAAAGAGCCTGCCACTCAGTCCCTCGGTGGCTCTAGCAGAGAGGGGAAGGGCCACA GTAAGAGTGGGCCGGATGTGTTTCCTGCTACCCCAGGCTCCCAGAACAAAGCCAGCGATGGGATTGGCCA GGGAGAAGGTGGGCCCTCTGTCCCACTGCACACTGACAGGGCTCCTCTAGACGCCAAGCCACAACCCACC AGTGGTGGGCGGCCCCTGGAGGTGCTGGAGAAGCCTGTGCATTTGCCAAGGCCGGGACACCCAGGGCCTA GTGAGCCAGCGGACCAGAAACTGTCCGCTGTTGGTGAAAAGCAAACCCTGTCTCCAAAGCACCCCAAACC ATCCACTGTGAAAGATTGCCCCACCCTGTGCAAACAGACAGACAACAGACAGACAGACAAAAGCCCGAGT CAGCCGGCCGCCAACACCGACAGAAGGGCGGAAGGGAAGAAATGCACTGAAGCACTTTATGCTCCAGCAG AGGGCGACAAGCTCGAGGCCGGCCTTTCCTTTGTGCATAGCGAGAACCGGTTGAAAGGCGCGGAGCGGCC AGCCGCGGGGGTGGGGAAGGGCTTCCCTGAGGCCAGAGGGAAAGGGCCCGGTCCCCAGAAGCCACCGACG GAGGCAGACAAGCCCAATGGCATGAAACGGTCCCCCTCAGCCACTGGGCAGAGTTCTTTCCGATCCACGG CCCTCCCGGAAAAGTCTCTGAGCTGCTCCTCCAGCTTCCCTGAAACCAGGGCCGGAGTTAGAGAGGCCTC TGCAGCCAGCAGCGACACCTCTTCTGCCAAGGCCGCCGGGGGCATGCTGGAGCTTCCAGCCCCCAGCAAC AGGGACCATAGGAAGGCTCAGCCTGCCGGGGAGGGCCGAACCCACATGACAAAGAGTGACTCCCTGCCCT CCTTCCGGGTCTCCACCCTGCCTCTGGAGTCACACCACCCCGACCCAAACACCATGGGCGGGGCCAGCCA CCGGGACAGGGCTCTCTCGGTGACTGCCACCGTAGGGGAAACCAAAGGGAAGGACCCTGCCCCAGCCCAG CCTCCCCCAGCTAGGAAACAGAACGTGGGCAGAGACGTGACCAAGCCATCCCCAGCCCCAAACACTGACC GCCCCATCTCTCTTTCTAATGAGAAGGACTTTGTGGTACGGCAGAGGCGGGGGAAAGAGAGTTTGCGTAG CAGCCCTCACAAAAAGGCCTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC219460 representing NM_015183 Red=Cloning site Green=Tags(s) MDESSILRRRGLQKELSLPRRGSLIDSQKWNCLVKRCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQ DSPRNFSPSASAHFSFARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLS KHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYS PDNVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFLELQHKLDKLLQEAHDRSESGELAFIKQLV RKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAHLGNYD SGTAETPETDESVSSSNASLKLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILR NQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVL ALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEY IAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQDLITL LLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDT NDEDFNVEIRQFSSCSHRFSKVFSSIDRITQNSAEEKEDSVDKTKSTTLPSTETLSWSSEYSEMQQLSTS NSSDTESNRHKLSSGLLPKLAISTEGEQDEAASCPGDPHEEPGKPALPPEECAQEEPEVTTPASTISSST LSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPASHMARQRLESTEKKKISGKVTKSLSASALSLMIPGD MFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYT VHHIVWNVEEGSPACQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPAR RNSYKSRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRS PTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLGGQRYRSGRRKSAGNIPLSPL ARTPSPTPQPTSPQRSPSPLLGHSLGNSKIAQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEK LSPSYGSDKKHLCSRKHSLEVTQEEVQREQSQREAPLQSLDENVCDVPPLSRARPVEQGCLKRPVSRKVG RQESVDDLDRDKLKAKVVVKKADGFPEKQESHQKSHGPGSDLENFALFKLEEREKKVYPKAVERSSTFEN KASMQEAPPLGSLLKDALHKQASVRASEGAMSDGRVPAEHRQGGGDFRRAPAPGTLQDGLCHSLDRGISG KGEGTEKSSQAKELLRCEKLDSKLANIDYLRKKMSLEDKEDNLCPVLKPKMTAGSHECLPGNPVRPTGGQ QEPPPASESRAFVSSTHAAQMSAVSFVPLKALTGRVDSGTEKPGLVAPESPVRKSPSEYKLEGRSVSCLK PIEGTLDIALLSGPQASKTELPSPESAQSPSPSGDVRASVPPVLPSSSGKKNDTTSARELSPSSLKMNKS YLLEPWFLPPSRGLQNSPAVSLPDPEFKRDRKGPHPTARSPGTVMESNPQQREGSSPKHQDHTTDPKLLT CLGQNLHSPDLARPRCPLPPEASPSREKPGLRESSERGPPTARSERSAARADTCREPSMELCFPETAKTS DNSKNLLSVGRTHPDFYTQTQAMEKAWAPGGKTNHKDGPGEARPPPRDNSSLHSAGIPCEKELGKVRRGV EPKPEALLARRSLQPPGIESEKSEKLSSFPSLQKDGAKEPERKEQPLQRHPSSIPPPPLTAKDLSSPAAR QHCSSPSHASGREPGAKPSTAEPSSSPQDPPKPVAAHSESSSHKPRPGPDPGPPKTKHPDRSLSSQKPSV GATKGKEPATQSLGGSSREGKGHSKSGPDVFPATPGSQNKASDGIGQGEGGPSVPLHTDRAPLDAKPQPT SGGRPLEVLEKPVHLPRPGHPGPSEPADQKLSAVGEKQTLSPKHPKPSTVKDCPTLCKQTDNRQTDKSPS QPAANTDRRAEGKKCTEALYAPAEGDKLEAGLSFVHSENRLKGAERPAAGVGKGFPEARGKGPGPQKPPT EADKPNGMKRSPSATGQSSFRSTALPEKSLSCSSSFPETRAGVREASAASSDTSSAKAAGGMLELPAPSN RDHRKAQPAGEGRTHMTKSDSLPSFRVSTLPLESHHPDPNTMGGASHRDRALSVTATVGETKGKDPAPAQ PPPARKQNVGRDVTKPSPAPNTDRPISLSNEKDFVVRQRRGKESLRSSPHKKAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_015183
ORF Size	7302 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_015183.3
RefSeq Size	9917 bp
RefSeq ORF	7305 bp
Locus ID	375449
UniProt ID	O15021
Protein Families	Druggable Genome, Protein Kinase
MW	265.4 kDa
Gene Summary	This gene encodes a member of the microtubule-associated serine/threonine protein kinases. The proteins in this family contain a domain that gives the kinase the ability to determine its own scaffold to control the effects of their kinase activities. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2014]

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