United States
Germany
Japan
United Kingdom
China
MUC20 (NM_001098516) Human Tagged ORF Clone
CAT＃: RC219610
- TrueORF®
MUC20 (Myc-DDK-tagged)-Human mucin 20, cell surface associated (MUC20), transcript variant S. Note: ORF is codon optimized
ORF Plasmid: tGFP
"NM_001098516" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 3,744.00
CNY 5,040.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MUC-20 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC219610 ORF sequence, codon optimized.
Due to the complexity of NM_001098516, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAACGGACGACACAGAAGTGCCCGCTATGACTCTAGCACCGGGCCACGCCGCTCTGGAAACTCAAA CGCTGAGCGCTGAGACCTCTTCTAGGGCCTCAACCCCAGCCGGCCCCATTCCAGAAGCAGAGACCAGGGG AGCCAAGAGAATTTCCCCTGCAAGAGAGACCAGGAGTTTCACAAAAACATCTCCCAACTTCATGGTGCTG ATCGCCACCTCCGTGGAGACATCAGCCGCCAGTGGCAGCCCCGAGGGAGCTGGAATGACCACAGTTCAGA CCATCACAGGCAGTGATCCCAGGGAAGCCATCTTTGACACCCTTTGCACCGATGACAGCTCTGAAGAGGC AAAGACACTCACAATGGACATATTGACATTGGCTCACACCTCCACAGAAGCTAAGGGCCTGTCCTCAGAG AGCAGCGCCTCTTCCGACAGCCCCCATCCAGTCATCACCCCGTCACGGGCCTCAGAGAGCAGCGCCTCTT CCGACGGCCCCCATCCAGTCATCACCCCGTCACGGGCCTCAGAGAGCAGCGCCTCTTCCGACGGCCCCCA TCCAGTCATCACCCCGTCATGGTCCCCGGGATCTGACGTCACTCTCCTCGCTGAAGCCCTGGTGACTGTC ACAAACATCGAGGTTATTAATTGCAGCATCACAGAAATAGAAACAACGACTTCCAGCATCCCTGGGGCCT CAGACACAGATCTCATCCCCACGGAAGGGGTGAAGGCCTCGTCCACCTCCGATCCACCAGCTCTGCCTGA CTCCACTGAAGCAAAACCACACATCACTGAGGTCACAGCCTCTGCCGAGACCCTGTCCACAGCCGGCACC ACAGAGTCAGCTGCACCTGATGCCACGGTTGGGACCCCACTCCCCACTAACAGCGCCACAGAAAGAGAAG TGACAGCACCCGGGGCCACGACCCTCAGTGGAGCTCTGGTCACAGTTAGCAGGAATCCCCTTGAAGAAAC CTCAGCCCTCTCTGTTGAGACACCAAGTTACGTCAAAGTCTCAGGAGCAGCTCCGGTCTCCATAGAGGCT GGGTCAGCAGTGGGCAAAACAACTTCCTTTGCTGGGAGCTCTGCTTCCTCCTACAGCCCCTCGGAAGCCG CCCTCAAGAACTTCACCCCTTCAGAGACACCGACCATGGACATCGCAACCAAGGGGCCCTTCCCCACCAG CAGGGACCCTCTTCCTTCTGTCCCTCCGACTACAACCAACAGCAGCCGAGGGACGAACAGCACCTTAGCC AAGATCACAACCTCAGCGAAGACCACGATGAAGCCCCCAACAGCCACGCCCACGACTGCCCGGACGAGGC CGACCACAGACGTGAGTGCAGGTGAAAATGGAGGTTTCCTCCTCCTGCGGCTGAGTGTGGCTTCCCCGGA AGACCTCACTGACCCCAGAGTGGCAGAAAGGCTGATGCAGCAGCTCCACCGGGAACTCCACGCCCACGCG CCTCACTTCCAGGTCTCCTTACTGCGTGTCAGGAGAGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219610 representing NM_001098516
Red=Cloning site Green=Tags(s) MTTDDTEVPAMTLAPGHAALETQTLSAETSSRASTPAGPIPEAETRGAKRISPARETRSFTKTSPNFMVL IATSVETSAASGSPEGAGMTTVQTITGSDPREAIFDTLCTDDSSEEAKTLTMDILTLAHTSTEAKGLSSE SSASSDSPHPVITPSRASESSASSDGPHPVITPSRASESSASSDGPHPVITPSWSPGSDVTLLAEALVTV TNIEVINCSITEIETTTSSIPGASDTDLIPTEGVKASSTSDPPALPDSTEAKPHITEVTASAETLSTAGT TESAAPDATVGTPLPTNSATEREVTAPGATTLSGALVTVSRNPLEETSALSVETPSYVKVSGAAPVSIEA GSAVGKTTSFAGSSASSYSPSEAALKNFTPSETPTMDIATKGPFPTSRDPLPSVPPTTTNSSRGTNSTLA KITTSAKTTMKPPTATPTTARTRPTTDVSAGENGGFLLLRLSVASPEDLTDPRVAERLMQQLHRELHAHA PHFQVSLLRVRRG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001098516 |
| ORF Size | 1509 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001098516.1, NP_001091986.1 |
| RefSeq Size | 6462 bp |
| RefSeq ORF | 1511 bp |
| Locus ID | 200958 |
| MW | 51.5 kDa |
| Gene Summary | This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins secreted by many epithelial tissues to form an insoluble mucous barrier. The C-terminus of this family member associates with the multifunctional docking site of the MET proto-oncogene and suppresses activation of some downstream MET signaling cascades. The protein features a mucin tandem repeat domain that varies between two and six copies in most individuals. Multiple variants encoding different isoforms have been found for this gene. A related pseudogene, which is also located on chromosome 3, has been identified. [provided by RefSeq, Apr 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219610L3 | Lenti-ORF clone of MUC20 (Myc-DDK-tagged)-Human mucin 20, cell surface associated (MUC20), transcript variant S. Note: ORF is codon optimized |
CNY 6,940.00 |
|
| RC219610L4 | Lenti-ORF clone of MUC20 (mGFP-tagged)-Human mucin 20, cell surface associated (MUC20), transcript variant S. Note: ORF is codon optimized |
CNY 6,940.00 |
|
| RG219610 | MUC20 (tGFP-tagged) - Human mucin 20, cell surface associated (MUC20), transcript variant S. Note: ORF is codon optimized |
CNY 5,510.00 |
|
| SC316396 | MUC20 (untagged)-Human mucin 20, cell surface associated (MUC20), transcript variant S |
CNY 7,220.00 |
