GPR64 (ADGRG2) (NM_005756) Human Tagged ORF Clone

CAT#: RC222702

  • TrueORF®

GPR64 (Myc-DDK-tagged)-Human G protein-coupled receptor 64 (GPR64), transcript variant 4

ORF Plasmid: DDK tGFP



  "NM_005756" in other vectors (4)

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CNY 10,070.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Anti-GPR64 Rabbit Polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms CBAVDX; EDDM6; GPR64; HE6; TM7LN2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC222702 representing NM_005756
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTTTTCTCTGTCAGGCAGTGTGGCCATGTTGGCAGAACTGAAGAAGTTTTACTGACGTTCAAGATAT
TCCTTGTCATCATTTGTCTTCATGTCGTTCTGGTAACATCCCTGGAAGAAGATACTGATAATTCCAGTTT
GTCACCACCACCTGCTAAATTATCTGTTGTCAGTTTTGCCCCCTCCTCCAATGAGGTTGAAACAACAAGC
CTCAATGATGTTACTTTAAGCTTACTCCCTTCAAACGAAACAGAAAAAACTAAAATCACTATAGTAAAAA
CCTTCAATGCATCAGGCGTCAAACCCCAGAGAAATATCTGCAATTTGTCATCTATTTGCAATGACTCAGC
ATTTTTTAGAGGTGAGATCATGTTTCAATATGATAAAGAAAGCACTGTTCCCCAGAATCAACATATAACG
AATGGCACCTTAACTGGAGTCCTGTCTCTAAGTGAATTAAAACGCTCAGAGCTCAACAAAACCCTGCAAA
CCCTAAGTGAGACTTACTTTATAATGTGTGCTACAGCAGAGGCCCAAAGCACATTAAATTGTACATTCAC
AATAAAACTGAATAATACAATGAATGCATGTGCTGTAATAGCTGCTTTGGAAAGAGTAAAGATTCGACCA
ATGGAACACTGCTGCTGTTCTGTCAGGATACCCTGCCCTTCCTCCCCAGAAGAGTTGGAAAAGCTTCAGT
GTGACCTGCAGGATCCCATTGTCTGTCTTGCTGACCATCCACGTGGCCCACCATTTTCTTCCAGCCAATC
CATCCCAGTGGTGCCTCGGGCCACTGTGCTTTCCCAGGTCCCCAAAGCTACCTCTTTTGCTGAGCCTCCA
GATTATTCACCTGTGACCCACAATGTTCCCTCTCCAATAGGGGAGATTCAACCCCTTTCACCCCAGCCTT
CAGCTCCCATAGCTTCCAGCCCTGCCATTGACATGCCCCCACAGTCTGAAACGATCTCTTCCCCTATGCC
CCAAACCCATGTCTCCGGCACCCCACCTCCTGTGAAAGCCTCATTTTCCTCTCCCACCGTGTCTGCCCCT
GCGAATGTCAACACTACCAGCGCACCTCCTGTCCAGACAGACATCGTCAACACCAGCAGTATTTCTGATC
TTGAGAACCAAGTGTTGCAGATGGAGAAGGCTCTGTCCTTGGGCAGCCTGGAGCCTAACCTCGCAGGAGA
AATGATCAACCAAGTCAGCAGACTCCTTCATTCCCCGCCTGACATGCTGGCCCCTCTGGCTCAAAGATTG
CTGAAAGTAGTGGATGACATTGGCCTACAGCTGAACTTTTCAAACACGACTATAAGTCTAACCTCCCCTT
CTTTGGCTCTGGCTGTGATCAGAGTGAATGCCAGTAGTTTCAACACAACTACCTTTGTGGCCCAAGACCC
TGCAAATCTTCAGGTTTCTCTGGAAACCCAAGCTCCTGAGAACAGTATTGGCACAATTACTCTTCCTTCA
TCGCTGATGAATAATTTACCAGCTCATGACATGGAGCTAGCTTCCAGGGTTCAGTTCAATTTTTTTGAAA
CACCTGCTTTGTTTCAGGATCCTTCCCTGGAGAACCTCTCTCTGATCAGCTACGTCATATCATCGAGTGT
TGCAAACCTGACCGTCAGGAACTTGACAAGAAACGTGACAGTCACATTAAAGCACATCAACCCGAGCCAG
GATGAGTTAACAGTGAGATGTGTATTTTGGGACTTGGGCAGAAATGGTGGCAGAGGAGGCTGGTCAGACA
ATGGCTGCTCTGTCAAAGACAGGAGATTGAATGAAACCATCTGTACCTGTAGCCATCTAACAAGCTTCGG
CGTTCTGCTGGACCTATCTAGGACATCTGTGCTGCCTGCTCAAATGATGGCTCTGACGTTCATTACATAT
ATTGGTTGTGGGCTTTCATCAATTTTTCTGTCAGTGACTCTTGTAACCTACATAGCTTTTGAAAAGATCC
GGAGGGATTACCCTTCCAAAATCCTCATCCAGCTGTGTGCTGCTCTGCTTCTGCTGAACCTGGTCTTCCT
CCTGGACTCGTGGATTGCTCTGTATAAGATGCAAGGCCTCTGCATCTCAGTGGCTGTATTTCTTCATTAT
TTTCTCTTGGTCTCATTCACATGGATGGGCCTAGAAGCATTCCATATGTACCTGGCCCTTGTCAAAGTAT
TTAATACTTACATCCGAAAATACATCCTTAAATTCTGCATTGTCGGTTGGGGGGTACCAGCTGTGGTTGT
GACCATCATCCTGACTATATCCCCAGATAACTATGGGCTTGGATCCTATGGGAAATTCCCCAATGGTTCA
CCGGATGACTTCTGCTGGATCAACAACAATGCAGTATTCTACATTACGGTGGTGGGATATTTCTGTGTGA
TATTTTTGCTGAACGTCAGCATGTTCATTGTGGTCCTGGTTCAGCTCTGTCGAATTAAAAAGAAGAAGCA
ACTGGGAGCCCAGCGAAAAACCAGTATTCAAGACCTCAGGAGTATCGCTGGCCTTACATTTTTACTGGGA
ATAACTTGGGGCTTTGCCTTCTTTGCCTGGGGACCAGTTAACGTGACCTTCATGTATCTGTTTGCCATCT
TTAATACCTTACAAGGATTTTTCATATTCATCTTTTACTGTGTGGCCAAAGAAAATGTCAGGAAGCAATG
GAGGCGGTATCTTTGTTGTGGAAAGTTACGGCTGGCTGAAAATTCTGACTGGAGTAAAACTGCTACTAAT
GGTTTAAAGAAGCAGACTGTAAACCAAGGAGTGTCCAGCTCTTCAAATTCCTTACAGTCAAGCAGTAACT
CCACTAACTCCACCACACTGCTAGTGAATAATGATTGCTCAGTACACGCAAGCGGGAATGGAAATGCTTC
TACAGAGAGGAATGGGGTCTCTTTTAGTGTTCAGAATGGAGATGTGTGCCTTCACGATTTCACTGGAAAA
CAGCACATGTTTAACGAGAAGGAAGATTCCTGCAATGGGAAAGGCCGTATGGCTCTCAGAAGGACTTCAA
AGCGGGGAAGCTTACACTTTATTGAGCAAATG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC222702 representing NM_005756
Red=Cloning site Green=Tags(s)

MVFSVRQCGHVGRTEEVLLTFKIFLVIICLHVVLVTSLEEDTDNSSLSPPPAKLSVVSFAPSSNEVETTS
LNDVTLSLLPSNETEKTKITIVKTFNASGVKPQRNICNLSSICNDSAFFRGEIMFQYDKESTVPQNQHIT
NGTLTGVLSLSELKRSELNKTLQTLSETYFIMCATAEAQSTLNCTFTIKLNNTMNACAVIAALERVKIRP
MEHCCCSVRIPCPSSPEELEKLQCDLQDPIVCLADHPRGPPFSSSQSIPVVPRATVLSQVPKATSFAEPP
DYSPVTHNVPSPIGEIQPLSPQPSAPIASSPAIDMPPQSETISSPMPQTHVSGTPPPVKASFSSPTVSAP
ANVNTTSAPPVQTDIVNTSSISDLENQVLQMEKALSLGSLEPNLAGEMINQVSRLLHSPPDMLAPLAQRL
LKVVDDIGLQLNFSNTTISLTSPSLALAVIRVNASSFNTTTFVAQDPANLQVSLETQAPENSIGTITLPS
SLMNNLPAHDMELASRVQFNFFETPALFQDPSLENLSLISYVISSSVANLTVRNLTRNVTVTLKHINPSQ
DELTVRCVFWDLGRNGGRGGWSDNGCSVKDRRLNETICTCSHLTSFGVLLDLSRTSVLPAQMMALTFITY
IGCGLSSIFLSVTLVTYIAFEKIRRDYPSKILIQLCAALLLLNLVFLLDSWIALYKMQGLCISVAVFLHY
FLLVSFTWMGLEAFHMYLALVKVFNTYIRKYILKFCIVGWGVPAVVVTIILTISPDNYGLGSYGKFPNGS
PDDFCWINNNAVFYITVVGYFCVIFLLNVSMFIVVLVQLCRIKKKKQLGAQRKTSIQDLRSIAGLTFLLG
ITWGFAFFAWGPVNVTFMYLFAIFNTLQGFFIFIFYCVAKENVRKQWRRYLCCGKLRLAENSDWSKTATN
GLKKQTVNQGVSSSSNSLQSSSNSTNSTTLLVNNDCSVHASGNGNASTERNGVSFSVQNGDVCLHDFTGK
QHMFNEKEDSCNGKGRMALRRTSKRGSLHFIEQM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_005756
ORF Size 3042 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005756.4
RefSeq Size 4665 bp
RefSeq ORF 3045 bp
Locus ID 10149
UniProt ID Q8IZP9
Domains GPS, 7tm_2
Protein Families Druggable Genome, GPCR, Transmembrane
MW 111.2 kDa
Gene Summary This gene encodes a member of the G protein-coupled receptor family described as an epididymis-specific transmembrane protein. The encoded protein may be proteolytically processed as it contains a motif shown to be a protein scission motif in some members of this family (PMID: 11973329). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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