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CDC2L1 (CDK11B) (NM_033493) Human Tagged ORF Clone
CAT＃: RC222810
- TrueORF®
CDK11B (Myc-DDK-tagged)-Human cyclin-dependent kinase 11B (CDK11B), transcript variant 9. Note: ORF is codon optimized
ORF Plasmid: tGFP
"NM_033493" in other vectors (4)
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CNY 5,648.00
CNY 7,700.00
Product images
CNY 300.00
CNY 2,700.00
CNY 3,790.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CDC2L1; CDK11; CDK11-p46; CDK11-p58; CDK11-p110; CLK-1; p58; p58CDC2L1; p58CLK-1; PK58 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC222810 ORF sequence, codon optimized.
Due to the complexity of NM_033493, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGATGAGAAAGACAGCTGGAAGGTAAAAACTCTTGACGAGATACTTCAGGAAAAAAAGCGCCGCA AGGAACAGGAAGAGAAGGCCGAGATAAAGCGGCTGAAGAACTCAGACGATAGAGATTCAAAGCGGGACAG CCTGGAAGAGGGTGAGTTGAGGGATCATTGTATGGAAATCACAATTCGAAACAGTCCGTACCGCCGCGAG GACAGTATGGAAGACCGGGGAGAGGAGGATGACTCATTGGCCATTAAGCCTCCTCAGCAGATGTCCCGCA AAGAGAAGGTGCATCATAGGAAAGACGAGAAGCGGAAGGAAAAAAAGCACGCGAGAGTTAAGGAAAAAGA GCGAGAGCATGAGCGACGGAAAAGGCATCGCGAGGAACAGGATAAAGCCAGGCGCGAGTGGGAACGCCAG AAGCGCCGCGAAATGGCCAGAGAACATTCTAGAAGAGAGAGAGATAGGCTCGAACAGCTGGAAAGGAAGA GAGAAAGAGAAAGGAAGATGAGGGAGCAGCAAAAGGAACAGAGGGAGCAAAAGGAAAGAGAGAGACGCGC GGAAGAGAGGAGAAAAGAAAGAGAAGCAAGACGCGAAGTGAGTGCCCATCACAGAACCATGCGGGAAGAT TACAGTGATAAAGTCAAGGCTTCCCACTGGTCACGATCCCCGCCCAGGCCACCTAGGGAGCGGTTCGAGC TGGGAGACGGGCGGAAGCCGGTTAAGGAAGAGAAAATGGAAGAACGAGATCTCCTTAGTGACCTGCAAGA TATATCTGACTCAGAGAGGAAAACCAGCTCCGCCGAGTCCAGTTCTGCCGAAAGCGGGTCAGGATCAGAG GAAGAGGAAGAGGAGGAGGAGGAGGAGGAGGAAGAGGGCTCTACAAGCGAGGAGAGCGAAGAGGAAGAGG AGGAGGAGGAGGAAGAAGAGGAGGAAACTGGTTCTAATTCAGAGGAGGCTTCAGAGCAGTCCGCTGAAGA GGTGTCCGAAGAGGAGATGTCTGAGGATGAAGAACGGGAGAACGAGAATCACCTCCTGGTGGTGCCAGAA TCCCGGTTCGATAGAGACAGTGGTGAAAGCGAGGAGGCCGAGGAGGAAGTCGGCGAGGGTACTCCCCAAT CCTCTGCCTTGACTGAAGGGGACTACGTCCCCGATTCCCCTGCTCTTTCCCCCATCGAGTTGAAGCAGGA GCTCCCCAAGTATCTGCCAGCCCTTCAGGGCTGCAGAAGTGTGGAGGAGTTTCAATGTCTCAATCGGATA GAAGAAGGCACCTATGGCGTTGTGTACAGAGCCAAAGACAAGAAAACAGACGAAATCGTTGCCCTGAAGC GACTGAAAATGGAGAAGGAAAAGGAAGGGTTCCCAATTACGAGCCTCCGCGAGATTAACACCATATTGAA GGCCCAGCATCCTAACATTGTTACAGTGAGGGAAATAGTGGTGGGCTCCAACATGGACAAAATCTACATA GTTATGAATTATGTGGAGCACGACCTCAAGTCTCTGATGGAAACAATGAAGCAGCCTTTCCTGCCCGGGG AGGTGAAAACATTGATGATACAACTTCTGAGAGGGGTCAAACATCTTCACGACAACTGGATCCTGCATCG AGATCTGAAGACATCTAACCTTCTGCTGTCCCATGCTGGTATCCTGAAGGTGGGAGACTTTGGGCTCGCC AGAGAGTATGGCAGCCCACTGAAGGCATACACCCCCGTTGTTGTGACCTTGTGGTACCGAGCACCTGAGT TGCTGCTTGGGGCGAAGGAGTATAGCACCGCCGTGGACATGTGGAGCGTCGGCTGTATCTTCGGCGAACT GCTGACCCAGAAGCCTTTGTTTCCGGGAAAATCCGAAATCGATCAAATCAATAAGGTGTTTAAAGACCTT GGGACTCCCTCTGAGAAGATCTGGCCAGGCTATTCTGAGCTCCCAGCAGTTAAGAAGATGACTTTTAGCG AGCATCCATATAACAACCTCAGGAAACGATTCGGCGCACTCCTCTCAGATCAGGGCTTCGACTTGATGAA TAAGTTTTTGACCTATTTCCCCGGACGCCGAATCTCTGCGGAAGATGGACTGAAGCATGAGTACTTCCGG GAGACACCATTGCCCATCGATCCCAGTATGTTCCCGACCTGGCCGGCAAAATCTGAACAGCAGAGGGTGA AGCGAGGTACCAGTCCTAGACCCCCTGAAGGGGGACTTGGTTACAGCCAGCTGGGAGATGACGACCTCAA GGAGACAGGTTTCCACCTGACAACTACCAACCAGGGCGCTTCAGCCGCCGGGCCAGGGTTTAGCTTGAAG TTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222810 representing NM_033493
Red=Cloning site Green=Tags(s) MGDEKDSWKVKTLDEILQEKKRRKEQEEKAEIKRLKNSDDRDSKRDSLEEGELRDHCMEITIRNSPYRRE DSMEDRGEEDDSLAIKPPQQMSRKEKVHHRKDEKRKEKKHARVKEKEREHERRKRHREEQDKARREWERQ KRREMAREHSRRERDRLEQLERKRERERKMREQQKEQREQKERERRAEERRKEREARREVSAHHRTMRED YSDKVKASHWSRSPPRPPRERFELGDGRKPVKEEKMEERDLLSDLQDISDSERKTSSAESSSAESGSGSE EEEEEEEEEEEEGSTSEESEEEEEEEEEEEEETGSNSEEASEQSAEEVSEEEMSEDEERENENHLLVVPE SRFDRDSGESEEAEEEVGEGTPQSSALTEGDYVPDSPALSPIELKQELPKYLPALQGCRSVEEFQCLNRI EEGTYGVVYRAKDKKTDEIVALKRLKMEKEKEGFPITSLREINTILKAQHPNIVTVREIVVGSNMDKIYI VMNYVEHDLKSLMETMKQPFLPGEVKTLMIQLLRGVKHLHDNWILHRDLKTSNLLLSHAGILKVGDFGLA REYGSPLKAYTPVVVTLWYRAPELLLGAKEYSTAVDMWSVGCIFGELLTQKPLFPGKSEIDQINKVFKDL GTPSEKIWPGYSELPAVKKMTFSEHPYNNLRKRFGALLSDQGFDLMNKFLTYFPGRRISAEDGLKHEYFR ETPLPIDPSMFPTWPAKSEQQRVKRGTSPRPPEGGLGYSQLGDDDLKETGFHLTTTNQGASAAGPGFSLK F TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_033493 |
| ORF Size | 2313 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_033493.1, NP_277028.1 |
| RefSeq Size | 2453 bp |
| RefSeq ORF | 2315 bp |
| Locus ID | 984 |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 90 kDa |
| Gene Summary | This gene encodes a member of the serine/threonine protein kinase family. Members of this kinase family are known to be essential for eukaryotic cell cycle control. Due to a segmental duplication, this gene shares very high sequence identity with a neighboring gene. These two genes are frequently deleted or altered in neuroblastoma. The protein kinase encoded by this gene can be cleaved by caspases and may play a role in cell apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222810L3 | Lenti-ORF clone of CDK11B (Myc-DDK-tagged)-Human cyclin-dependent kinase 11B (CDK11B), transcript variant 9. Note: ORF is codon optimized |
CNY 9,600.00 |
|
| RC222810L4 | Lenti-ORF clone of CDK11B (mGFP-tagged)-Human cyclin-dependent kinase 11B (CDK11B), transcript variant 9. Note: ORF is codon optimized |
CNY 9,600.00 |
|
| RG222810 | CDK11B (tGFP-tagged) - Human cyclin-dependent kinase 11B (CDK11B), transcript variant 9. Note: ORF is codon optimized |
CNY 8,460.00 |
|
| SC309471 | CDK11B (untagged)-Human cyclin-dependent kinase 11B (CDK11B), transcript variant 9 |
CNY 14,920.00 |
