United States
Germany
Japan
United Kingdom
China
GRIK1 (NM_000830) Human Tagged ORF Clone
CAT＃: RC222898
- TrueORF®
GRIK1 (Myc-DDK-tagged)-Human glutamate receptor, ionotropic, kainate 1 (GRIK1), transcript variant 1
ORF Plasmid: tGFP
"NM_000830" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 10,080.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | EAA3; EEA3; GLR5; GluK1; gluR-5; GLUR5 |
| Vector | pCMV6-AC-Myc-DDK |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC222898 representing NM_000830.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGCACGGCACACTCCTCGCCCAGCCCGGGCTCTGGACCAGGGACACCAGCTGGGCACTCCTCTAT TTCCTCTGCTATATCCTCCCTCAGACCGCCCCGCAAGTACTCAGGATCGGAGGGATTTTTGAAACAGTG GAAAATGAGCCTGTTAATGTTGAAGAATTAGCTTTCAAGTTTGCAGTCACCAGCATTAACAGAAACCGA ACCCTGATGCCTAACACCACATTAACCTATGACATCCAGAGAATTAACCTTTTTGATAGTTTTGAAGCC TCGCGGAGAGCATGTGACCAGCTGGCTCTTGGTGTGGCTGCTCTCTTTGGCCCTTCCCATAGCTCCTCC GTCAGTGCTGTGCAGTCTATTTGCAATGCTCTCGAAGTTCCACACATACAGACCCGCTGGAAACACCCC TCGGTGGACAACAAAGATTTGTTTTACATCAACCTTTACCCAGATTATGCAGCTATCAGCAGGGCGATC CTGGATCTGGTCCTCTATTACAACTGGAAAACAGTGACAGTGGTGTATGAAGACAGCACAGGTCTAATT CGTCTACAAGAGCTCATCAAAGCTCCCTCCAGATATAATATTAAAATCAAAATCCGCCAGCTGCCCTCT GGGAATAAAGATGCCAAGCCTTTACTCAAGGAGATGAAGAAAGGCAAGGAGTTCTATGTGATATTTGAT TGTTCACATGAAACAGCCGCTGAAATCCTTAAGCAGATTCTGTTCATGGGCATGATGACCGAGTACTAT CACTACTTTTTCACAACCCTGGACTTATTTGCTTTGGATCTGGAACTCTATAGGTACAGTGGCGTAAAC ATGACCGGGTTTCGGCTGCTTAACATTGACAACCCTCACGTGTCATCCATCATTGAGAAGTGGTCCATG GAGAGACTGCAGGCCCCACCCAGGCCCGAGACTGGCCTTTTGGATGGCATGATGACAACTGAAGCGGCT CTGATGTACGATGCTGTGTACATGGTGGCCATTGCCTCGCACCGGGCATCCCAGCTGACCGTCAGCTCC CTGCAGTGCCATAGACATAAGCCATGGCGCCTCGGACCCAGATTTATGAACCTGATCAAAGAGGCCCGG TGGGATGGCTTGACTGGGCATATCACCTTTAATAAAACCAATGGCTTGAGGAAGGATTTTGATCTGGAC ATTATTAGTCTCAAAGAGGAAGGAACTGAAAAGGCTGCTGGCGAAGTGTCTAAACACTTGTATAAAGTG TGGAAGAAGATTGGGATTTGGAATTCCAACAGTGGGCTTAACATGACGGACAGCAACAAAGACAAGTCC AGCAATATCACTGATTCATTGGCCAACAGAACACTCATTGTCACCACCATTCTGGAAGAACCCTATGTT ATGTACAGGAAATCTGATAAGCCTCTATATGGAAATGACAGATTTGAAGGATATTGCCTAGACCTGTTG AAAGAATTGTCAAACATCCTGGGTTTCATTTATGATGTTAAACTAGTTCCCGATGGCAAATATGGGGCC CAGAATGACAAAGGGGAGTGGAACGGGATGGTTAAAGAACTCATAGATCACAGGGCTGACCTGGCAGTG GCTCCTCTTACCATCACCTACGTGCGGGAGAAAGTCATTGACTTCTCCAAACCCTTCATGACCCTAGGC ATCAGCATTCTCTACCGGAAGCCCAATGGTACCAATCCAGGCGTTTTCTCCTTCCTCAACCCCCTGTCT CCAGATATTTGGATGTATGTGCTCTTAGCCTGCTTGGGAGTCAGCTGTGTACTCTTTGTGATTGCAAGG TTTACACCCTACGAGTGGTATAACCCCCACCCATGCAACCCTGACTCAGACGTGGTGGAAAACAATTTT ACTTTACTAAATAGTTTCTGGTTTGGAGTTGGAGCTCTCATGCAGCAAGGATCAGAGCTGATGCCCAAA GCTCTATCGACCAGAATAGTTGGAGGGATATGGTGGTTTTTCACCCTAATCATCATTTCATCCTACACG GCCAATCTGGCTGCCTTCTTGACAGTAGAGAGAATGGAATCCCCCATAGATTCGGCAGATGATCTGGCA AAGCAAACCAAGATAGAATATGGGGCGGTTAGAGATGGATCAACAATGACCTTCTTCAAGAAATCAAAA ATCTCCACCTATGAGAAGATGTGGGCTTTCATGAGCAGCAGGCAGCAGACCGCCCTGGTAAGAAACAGT GATGAGGGGATCCAGAGAGTGCTCACCACAGACTACGCGCTGCTGATGGAGTCCACCAGCATTGAGTAT GTGACGCAGAGAAACTGCAACCTCACTCAGATCGGGGGCCTCATTGACTCCAAAGGTTACGGAGTGGGA ACACCTATTGGTTCTCCTTACCGGGATAAAATTACTATTGCTATTCTTCAACTCCAAGAAGAAGGGAAG CTGCATATGATGAAAGAGAAGTGGTGGCGTGGGAATGGCTGCCCCGAGGAAGACAACAAAGAAGCCAGT GCCCTGGGAGTGGAAAATATTGGAGGCATCTTCATTGTTCTGGCTGCCGGACTGGTCCTTTCTGTATTT GTAGCTATTGGAGAATTCATATACAAATCACGGAAGAATAATGATATTGAACAGGCTTTTTGTTTCTTT TATGGACTGCAATGTAAGCAAACCCATCCAACCAACTCCACTTCTGGAACTACTTTATCTACGGATTTA GAATGTGGTAAATTAATTCGAGAGGAGAGAGGGATTCGAAAACAGTCCTCAGTTCATACTGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC >Peptide sequence encoded by RC222898
Blue=ORF Red=Cloning site Green=Tag(s) MEHGTLLAQPGLWTRDTSWALLYFLCYILPQTAPQVLRIGGIFETVENEPVNVEELAFKFAVTSINRNR TLMPNTTLTYDIQRINLFDSFEASRRACDQLALGVAALFGPSHSSSVSAVQSICNALEVPHIQTRWKHP SVDNKDLFYINLYPDYAAISRAILDLVLYYNWKTVTVVYEDSTGLIRLQELIKAPSRYNIKIKIRQLPS GNKDAKPLLKEMKKGKEFYVIFDCSHETAAEILKQILFMGMMTEYYHYFFTTLDLFALDLELYRYSGVN MTGFRLLNIDNPHVSSIIEKWSMERLQAPPRPETGLLDGMMTTEAALMYDAVYMVAIASHRASQLTVSS LQCHRHKPWRLGPRFMNLIKEARWDGLTGHITFNKTNGLRKDFDLDIISLKEEGTEKAAGEVSKHLYKV WKKIGIWNSNSGLNMTDSNKDKSSNITDSLANRTLIVTTILEEPYVMYRKSDKPLYGNDRFEGYCLDLL KELSNILGFIYDVKLVPDGKYGAQNDKGEWNGMVKELIDHRADLAVAPLTITYVREKVIDFSKPFMTLG ISILYRKPNGTNPGVFSFLNPLSPDIWMYVLLACLGVSCVLFVIARFTPYEWYNPHPCNPDSDVVENNF TLLNSFWFGVGALMQQGSELMPKALSTRIVGGIWWFFTLIIISSYTANLAAFLTVERMESPIDSADDLA KQTKIEYGAVRDGSTMTFFKKSKISTYEKMWAFMSSRQQTALVRNSDEGIQRVLTTDYALLMESTSIEY VTQRNCNLTQIGGLIDSKGYGVGTPIGSPYRDKITIAILQLQEEGKLHMMKEKWWRGNGCPEEDNKEAS ALGVENIGGIFIVLAAGLVLSVFVAIGEFIYKSRKNNDIEQAFCFFYGLQCKQTHPTNSTSGTTLSTDL ECGKLIREERGIRKQSSVHTV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000830 |
| ORF Size | 2754 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000830.6 |
| RefSeq Size | 3231 bp |
| RefSeq ORF | 2757 bp |
| Locus ID | 2897 |
| UniProt ID | P39086 |
| Domains | lig_chan, ANF_receptor |
| Protein Families | Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane |
| Protein Pathways | Neuroactive ligand-receptor interaction |
| MW | 104 kDa |
| Gene Summary | Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. The subunit encoded by this gene is subject to RNA editing (CAG->CGG; Q->R) within the second transmembrane domain, which is thought to alter the properties of ion flow. Alternative splicing, resulting in transcript variants encoding different isoforms, has been noted for this gene. [provided by RefSeq, Jul 2008] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Ultrarare Loss-of-Function Mutations in the Genes Encoding the Ionotropic Glutamate Receptors of Kainate Subtypes Associated with Schizophrenia Disrupt the Interaction with PSD95
,null,
Journal of Personalized Medicine
,PubMed ID 35629206
[GRIK1]
|
|
Encephalitis with Autoantibodies against the Glutamate Kainate Receptors GluK2
,Landa, J;Guasp, M;Míguez-Cabello, F;Guimarães, J;Mishima, T;Oda, F;Zipp, F;Krajinovic, V;Fuhr, P;Honnorat, J;Titulaer, M;Simabukuro, M;Planagumà, J;Martínez-Hernández, E;Armangué, T;Saiz, A;Gasull, X;Soto, D;Graus, F;Sabater, L;Dalmau, J;GluK2 encephalitis study group, ;,
Annals of neurology
,PubMed ID 33949707
[GRIK1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222898L1 | Lenti-ORF clone of GRIK1 (Myc-DDK-tagged)-Human glutamate receptor, ionotropic, kainate 1 (GRIK1), transcript variant 1 |
CNY 11,500.00 |
|
| RC222898L2 | Lenti-ORF clone of GRIK1 (mGFP-tagged)-Human glutamate receptor, ionotropic, kainate 1 (GRIK1), transcript variant 1 |
CNY 11,500.00 |
|
| RC222898L3 | Lenti-ORF clone of GRIK1 (Myc-DDK-tagged)-Human glutamate receptor, ionotropic, kainate 1 (GRIK1), transcript variant 1 |
CNY 11,500.00 |
|
| RC222898L4 | Lenti-ORF clone of GRIK1 (mGFP-tagged)-Human glutamate receptor, ionotropic, kainate 1 (GRIK1), transcript variant 1 |
CNY 11,500.00 |
|
| RG222898 | GRIK1 (tGFP-tagged) - Human glutamate receptor, ionotropic, kainate 1 (GRIK1), transcript variant 1 |
CNY 11,680.00 |
|
| SC122079 | GRIK1 (untagged)-Human glutamate receptor, ionotropic, kainate 1 (GRIK1), transcript variant 1 |
CNY 10,096.00 |
