Elastin (ELN) (NM_001081752) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ADCL1; SVAS; WBS; WS
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC224648 ORF sequence, codon optimized. Due to the complexity of NM_001081752, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGGCCTGACAGCCGCTGCCCCAAGGCCCGGCGTGCTGTTGCTTCTGCTGTCTATTCTGCACCCTT CTAGACCTGGGGGGGTACCGGGGGCCATTCCCGGTGGCGTCCCAGGCGGGGTGTTTTACCCCGCACTGGG CCCAGGGGGCAAGCCTCTGAAGCCTGTGCCCGGTGGCCTCGCTGGGGCTGGCCTCGGTGCGGGCTTGGGC GCGTTTCCCGCTGTCACCTTCCCTGGTGCCCTGGTACCAGGCGGCGTGGCCGACGCTGCGGCCGCGTACA AAGCCGCTAAGGCGGGAGCAGGACTCGGGGGAGTACCCGGGGTAGGTGGCCTCGGCGTAAGCGCTGGAGC CGTCGTGCCTCAGCCTGGCGCCGGAGTCAAACCTGGGAAGGTGCCCGGGGTAGGGCTGCCTGGAGTGTAC CCAGGCGGAGTTCTCCCCGGCGCCCGCTTCCCCGGGGTGGGAGTCCTGCCAGGGGTGCCAACAGGAGCCG GAGTGAAACCCAAAGCACCTGGCGTCGGCGGTGCGTTCGCCGGTATCCCTGGGGTAGGTCCTTTCGGGGG CCCTCAACCCGGTGTGCCGCTTGGTTATCCCATCAAAGCCCCAAAACTTCCAGGTGGGTACGGCTTGCCA TATACAACAGGCAAGCTCCCCTATGGCTACGGACCGGGCGGGGTGGCCGGGGCAGCCGGCAAGGCTGGAT ACCCCACCGGCACAGGAGTGGGGCCACAGGCGGCAGCGGCCGCCGCTGCCAAGGCAGCTGCCAAATTTGG CGCGGGCGCCGCCGGAGTCCTCCCTGGGGTTGGCGGTGCCGGCGTACCTGGGGTACCCGGCGCCATCCCC GGGATTGGAGGAATTGCCGGTGTCGGCACACCCGCAGCAGCCGCCGCCGCCGCCGCGGCGGCCAAGGCAG CCAAATACGGTGCCGCTGCAGGGCTGGTACCTGGAGGACCAGGTTTTGGACCCGGAGTCGTCGGAGTTCC CGGGGCGGGAGTCCCTGGCGTCGGGGTTCCCGGCGCCGGGATCCCCGTAGTCCCCGGAGCTGGAATTCCT GGCGCCGCCGTGCCTGGAGTTGTGAGTCCTGAAGCAGCCGCAAAAGCTGCCGCCAAAGCTGCTAAGTATG GCGCCCGCCCCGGCGTCGGAGTGGGAGGCATTCCTACATATGGCGTGGGAGCCGGCGGGTTCCCTGGATT CGGCGTGGGTGTCGGCGGAATTCCCGGCGTGGCCGGCGTTCCTGGCGTTGGGGGAGTTCCCGGTGTTGGC GGGGTTCCTGGCGTGGGGATCAGCCCAGAAGCCCAAGCCGCTGCCGCTGCGAAAGCCGCCAAGTATGGAC TGGTTCCGGGAGTCGGGGTCGCACCGGGCGTTGGAGTTGCCCCTGGCGTGGGAGTGGCACCTGGGGTGGG GCTGGCCCCCGGTGTGGGAGTTGCTCCCGGTGTAGGAGTGGCTCCAGGTGTGGGCGTAGCACCCGGCATT GGTCCCGGGGGCGTGGCCGCGGCCGCCAAATCTGCGGCCAAGGTTGCCGCCAAAGCACAATTGAGGGCAG CCGCTGGTTTGGGAGCTGGTATCCCTGGGCTGGGTGTGGGAGTGGGAGTACCCGGTCTTGGTGTGGGAGC GGGGGTTCCAGGACTCGGCGTGGGCGCCGGCGTCCCTGGATTTGGTGCAGTGCCGGGTGCCCTCGCAGCT GCAAAAGCGGCTAAATACGGGGCGGCAGTCCCTGGAGTGCTGGGGGGCCTTGGCGCCTTGGGAGGCGTCG GAATACCTGGAGGGGTTGTGGGCGCTGGACCAGCTGCCGCAGCCGCAGCCGCCAAGGCCGCTGCTAAGGC TGCCCAGTTCGGCCTCGTGGGGGCCGCCGGATTGGGAGGGCTTGGCGTCGGTGGCCTCGGAGTGCCCGGG GTGGGAGGGCTCGGTGGCATTCCTCCCGCTGCAGCAGCAAAAGCCGCCAAATACGGAGTGGCAGCCAGAC CTGGGTTCGGCCTGAGTCCAATCTTTCCAGGAGGCGCTTGCCTCGGTAAAGCTTGCGGAAGAAAGCGCAA G ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC224648 representing NM_001081752 Red=Cloning site Green=Tags(s) MAGLTAAAPRPGVLLLLLSILHPSRPGGVPGAIPGGVPGGVFYPALGPGGKPLKPVPGGLAGAGLGAGLG AFPAVTFPGALVPGGVADAAAAYKAAKAGAGLGGVPGVGGLGVSAGAVVPQPGAGVKPGKVPGVGLPGVY PGGVLPGARFPGVGVLPGVPTGAGVKPKAPGVGGAFAGIPGVGPFGGPQPGVPLGYPIKAPKLPGGYGLP YTTGKLPYGYGPGGVAGAAGKAGYPTGTGVGPQAAAAAAAKAAAKFGAGAAGVLPGVGGAGVPGVPGAIP GIGGIAGVGTPAAAAAAAAAAKAAKYGAAAGLVPGGPGFGPGVVGVPGAGVPGVGVPGAGIPVVPGAGIP GAAVPGVVSPEAAAKAAAKAAKYGARPGVGVGGIPTYGVGAGGFPGFGVGVGGIPGVAGVPGVGGVPGVG GVPGVGISPEAQAAAAAKAAKYGLVPGVGVAPGVGVAPGVGVAPGVGLAPGVGVAPGVGVAPGVGVAPGI GPGGVAAAAKSAAKVAAKAQLRAAAGLGAGIPGLGVGVGVPGLGVGAGVPGLGVGAGVPGFGAVPGALAA AKAAKYGAAVPGVLGGLGALGGVGIPGGVVGAGPAAAAAAAKAAAKAAQFGLVGAAGLGGLGVGGLGVPG VGGLGGIPPAAAAKAAKYGVAARPGFGLSPIFPGGACLGKACGRKRK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001081752
ORF Size	2031 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001081752.1, NM_001081752.2, NP_001075221.1
RefSeq Size	3648 bp
RefSeq ORF	2034 bp
Locus ID	2006
UniProt ID	P15502
Protein Families	Druggable Genome, Secreted Protein, Transmembrane
MW	58.8 kDa
Gene Summary	This gene encodes a protein that is one of the two components of elastic fibers. Elastic fibers comprise part of the extracellular matrix and confer elasticity to organs and tissues including the heart, skin, lungs, ligaments, and blood vessels. The encoded protein is rich in hydrophobic amino acids such as glycine and proline, which form mobile hydrophobic regions bounded by crosslinks between lysine residues. Degradation products of the encoded protein, known as elastin-derived peptides or elastokines, bind the elastin receptor complex and other receptors and stimulate migration and proliferation of monocytes and skin fibroblasts. Elastokines can also contribute to cancer progression. Deletions and mutations in this gene are associated with supravalvular aortic stenosis (SVAS) and autosomal dominant cutis laxa. [provided by RefSeq, Aug 2017]

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