Apg10 (ATG10) (NM_001131028) Human Tagged ORF Clone
CAT#: RC225262
ATG10 (Myc-DDK-tagged)-Human ATG10 autophagy related 10 homolog (S. cerevisiae) (ATG10), transcript variant 3
ORF Plasmid: tGFP
"NM_001131028" in other vectors (4)
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CNY 2,400.00
CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | APG10; APG10L; pp12616 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC225262 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGAAGATGAGTTCATTGGAGAAAAAACATTCCAACGTTATTGTGCAGAATTCATTAAACATTCAC AACAGATAGGTGATAGTTGGGAATGGAGACCATCAAAGGACTGTTCTGATGGCTACATGTGCAAAATACA CTTTCAAATTAAGAATGGGTCTGTGATGTCACATCTAGGAGCATCTACCCATGGACAGACATGTCTTCCC ATGGAGGAGGCTTTCGAGCTACCCTTGGATGATTGTGAAGTGATTGAAACTGCAGCAGCGTCCGAAGTGA TTAAATATGAGTATCATGTCTTATATTCCTGTAGCTACCAAGTGCCTGTACTTTACTTTAGGGCAAGCTT TTTAGATGGGAGACCTTTAACTCTGAAGGACATATGGGAAGGAGTTCATGAGTGCTATAAGATGCGACTG CTACAGGGACCATGGGACACTATTACGCAACAGGAACATCCAATACTTGGGCAACCCTTTTTTGTACTTC ATCCCTGCAAGACGAATGAATTCATGACTCCTGTATTAAAGAATTCTCAGAAAATCAATAAGAATGTCAA CTATATCACATCATGGCTGAGCATTGTAGGGCCAGTTGTTGGGCTGAATCTACCTCTGAGTTATGCCAAA GCAACGTCTCAGGATGAACGAAATGTCCCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC225262 protein sequence
Red=Cloning site Green=Tags(s) MEEDEFIGEKTFQRYCAEFIKHSQQIGDSWEWRPSKDCSDGYMCKIHFQIKNGSVMSHLGASTHGQTCLP MEEAFELPLDDCEVIETAAASEVIKYEYHVLYSCSYQVPVLYFRASFLDGRPLTLKDIWEGVHECYKMRL LQGPWDTITQQEHPILGQPFFVLHPCKTNEFMTPVLKNSQKINKNVNYITSWLSIVGPVVGLNLPLSYAK ATSQDERNVP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001131028 |
ORF Size | 660 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001131028.2 |
RefSeq Size | 2432 bp |
RefSeq ORF | 663 bp |
Locus ID | 83734 |
UniProt ID | Q9H0Y0 |
MW | 25.3 kDa |
Gene Summary | Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).[supplied by OMIM, Mar 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC225262L3 | Lenti ORF clone of Human ATG10 autophagy related 10 homolog (S. cerevisiae) (ATG10), transcript variant 3, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC225262L4 | Lenti ORF clone of Human ATG10 autophagy related 10 homolog (S. cerevisiae) (ATG10), transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
RG225262 | ATG10 (tGFP-tagged) - Human ATG10 autophagy related 10 homolog (S. cerevisiae) (ATG10), transcript variant 3 |
CNY 4,370.00 |
|
SC324798 | ATG10 (untagged)-Human ATG10 autophagy related 10 homolog (S. cerevisiae) (ATG10), transcript variant 3 |
CNY 3,990.00 |