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PHF8 (NM_001184898) Human Tagged ORF Clone
CAT＃: RC230556
- TrueORF®
PHF8 (Myc-DDK-tagged)-Human PHD finger protein 8 (PHF8), transcript variant 4
ORF Plasmid: tGFP
"NM_001184898" in other vectors (4)
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CNY 8,740.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | JHDM1F; KDM7B; MRXSSD; ZNF422 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC230556 representing NM_001184898
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCGGTGCCGGTGTATTGCCTCTGCCGGCTGCCTTACGATGTGACCCGCTTCATGATCGAGTGTG ACATGTGCCAGGACTGGTTTCATGGCAGTTGTGTTGGTGTTGAAGAGGAGAAGGCTGCTGACATTGACCT CTACCACTGCCCCAACTGTGAAGTCTTGCATGGGCCCTCCATTATGAAAAAACGCCGTGGATCTTCAAAG GGGCATGATACACACAAGGGGAAACCAGTGAAGACCGGGAGCCCTACGTTCGTCAGAGAGCTCCGGAGTA GGACTTTTGACAGCTCAGATGAAGTGATTCTGAAGCCCACTGGAAATCAACTGACCGTGGAATTCCTGGA AGAAAATAGCTTCAGTGTGCCCATCCTGGTCCTGAAGAAGGATGGGTTGGGCATGACGCTGCCCTCGCCA TCATTCACTGTGAGGGATGTTGAACACTATGTTGGTTCTGACAAAGAGATTGATGTGATTGATGTGACCC GCCAGGCTGACTGCAAGATGAAGCTTGGTGATTTTGTGAAATACTATTACAGCGGGAAGAGGGAGAAAGT CCTCAATGTCATTAGTTTGGAATTCTCTGATACCAGACTTTCTAACCTTGTGGAGACACCGAAGATTGTT CGAAAGCTGTCATGGGTCGAAAACTTGTGGCCAGAGGAATGTGTCTTTGAGAGACCCAATGTACAGAAGT ACTGCCTCATGAGTGTGCGAGATAGCTATACAGACTTTCACATTGACTTTGGTGGCACCTCTGTCTGGTA CCATGTACTCAAGGGTGAAAAGATCTTCTACCTGATCCGCCCAACAAATGCCAATCTGACTCTCTTTGAG TGCTGGAGCAGTTCCTCTAATCAGAATGAGATGTTCTTTGGGGACCAGGTGGACAAGTGCTACAAGTGTT CCGTGAAGCAAGGACAGACACTTTTCATTCCCACAGGGTGGATCCATGCTGTGCTGACGCCTGTGGACTG CCTTGCCTTTGGAGGGAACTTCTTACACAGCCTTAACATCGAGATGCAGCTCAAAGCCTATGAGATTGAG AAGCGGCTGAGCACAGCAGACCTCTTCAGATTCCCCAACTTTGAGACCATCTGTTGGTATGTGGGAAAGC ACATCCTGGACATCTTTCGCGGTTTGCGAGAGAACAGGAGACACCCTGCCTCCTACCTGGTCCATGGTGG CAAAGCCTTGAACTTGGCCTTTAGAGCCTGGACAAGGAAAGAAGCTCTGCCAGACCATGAGGATGAGATC CCGGAGACAGTGCGAACCGTACAGCTCATTAAAGATCTGGCCAGGGAGATCCGCCTGGTGGAAGACATCT TCCAACAGAACGTTGGGAAGACGAGCAATATCTTTGGGCTGCAGAGGATCTTCCCAGCCGGCTCCATTCC CCTAACCAGGCCAGCCCATTCCACTTCAGTGTCCATGTCCAGGCTGTCACTGCCCTCCAAAAATGGTTCA AAGAAGAAAGGCCTGAAGCCCAAGGAACTCTTCAAGAAGGCAGAGCGAAAGGGCAAGGAGAGTTCAGCCT TGGGGCCTGCTGGCCAGTTGAGCTATAATCTCATGGACACATACAGTCATCAGGCACTGAAGACAGGCTC TTTCCAGAAAGCAAAGTTCAACATCACTGGTGCCTGCTTGAATGACTCAGATGACGACTCACCAGACTTG GACCTTGATGGAAATGAGAGCCCATTGGCCCTATTGATGTCTAACGGCAGTACGAAAAGGGTGAAGAGTT TATCCAAATCTCGGCGAACCAAGATAGCAAAGAAGGTAGACAAGGCTAGGCTGATGGCAGAACAGGTGAT GGAAGACGAATTTGACTTGGATTCAGATGATGAGCTGCAGATTGACGAGAGATTGGGAAAGGAGAAGGCG ACCCTGATAATAAGACCAAAATTTCCCCGGAAATTGCCCCGTGCGAAGCCTTGCTCTGACCCCAACCGAG TTCGTGAACCAGGAGAAGTTGAGTTTGACATTGAGGAGGACTATACAACAGATGAGGACATGGTGGAAGG GGTTGAAGGCTATCAGACAGCAACCCCCGCTCCCGCCCAAGGTGCCAGCGAGGCCCCAGCTTCTCCCAGC ACTCAGGAGGCCATCCAGGGCATGCTGTGCATGGCCAACCTGCAGTCCTCATCGTCCTCACCGGCTACCT CTAGCCTGCAGGCCTGGTGGACTGGGGGACAGGATCGAAGCAGTGGGAGCTCCAGCAGTGGGCTGGGCAC AGTGTCTAACAGTCCTGCTTCCCAGCGCACCCCAGGGAAGCGGCCCATCAAGCGGCCAGCATACTGGAGA ACCGAGAGCGAGGAGGAGGAGGAGAACGCCAGTCTGGATGAACAGGACAGCTTGGGAGCGTGCTTCAAGG ATGCAGAGTATATCTATCCTTCACTGGAGTCTGATGATGATGACCCTGCTTTGAAATCTCGACCCAAGAA AAAGAAGAATTCAGATGATGCTCCATGGAGTCCTAAAGCCCGCGTGACCCCAACTCTGCCGAAGCAGGAC CGTCCTGTGCGTGAGGGGACCCGGGTAGCCTCTATTGAGACAGGTTTGGCTGCAGCAGCTGCAAAGCTGG CCCAGCAGGTGAAGAAAATGAAGCTCTCATTAACTGACTCAGGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC230556 representing NM_001184898
Red=Cloning site Green=Tags(s) MASVPVYCLCRLPYDVTRFMIECDMCQDWFHGSCVGVEEEKAADIDLYHCPNCEVLHGPSIMKKRRGSSK GHDTHKGKPVKTGSPTFVRELRSRTFDSSDEVILKPTGNQLTVEFLEENSFSVPILVLKKDGLGMTLPSP SFTVRDVEHYVGSDKEIDVIDVTRQADCKMKLGDFVKYYYSGKREKVLNVISLEFSDTRLSNLVETPKIV RKLSWVENLWPEECVFERPNVQKYCLMSVRDSYTDFHIDFGGTSVWYHVLKGEKIFYLIRPTNANLTLFE CWSSSSNQNEMFFGDQVDKCYKCSVKQGQTLFIPTGWIHAVLTPVDCLAFGGNFLHSLNIEMQLKAYEIE KRLSTADLFRFPNFETICWYVGKHILDIFRGLRENRRHPASYLVHGGKALNLAFRAWTRKEALPDHEDEI PETVRTVQLIKDLAREIRLVEDIFQQNVGKTSNIFGLQRIFPAGSIPLTRPAHSTSVSMSRLSLPSKNGS KKKGLKPKELFKKAERKGKESSALGPAGQLSYNLMDTYSHQALKTGSFQKAKFNITGACLNDSDDDSPDL DLDGNESPLALLMSNGSTKRVKSLSKSRRTKIAKKVDKARLMAEQVMEDEFDLDSDDELQIDERLGKEKA TLIIRPKFPRKLPRAKPCSDPNRVREPGEVEFDIEEDYTTDEDMVEGVEGYQTATPAPAQGASEAPASPS TQEAIQGMLCMANLQSSSSSPATSSLQAWWTGGQDRSSGSSSSGLGTVSNSPASQRTPGKRPIKRPAYWR TESEEEEENASLDEQDSLGACFKDAEYIYPSLESDDDDPALKSRPKKKKNSDDAPWSPKARVTPTLPKQD RPVREGTRVASIETGLAAAAAKLAQQVKKMKLSLTDSG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001184898 |
| ORF Size | 2634 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001184898.2 |
| RefSeq ORF | 2637 bp |
| Locus ID | 23133 |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 98.7 kDa |
| Gene Summary | The protein encoded by this gene is a histone lysine demethylase that preferentially acts on histones in the monomethyl or dimethyl states. The encoded protein requires Fe(2+) ion, 2-oxoglutarate, and oxygen for its catalytic activity. The protein has an N-terminal PHD finger and a central Jumonji C domain. This gene is thought to function as a transcription activator. Defects in this gene are a cause of syndromic X-linked Siderius type intellectual disability (MRXSSD) and over-expression of this gene is associated with several forms of cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230556L3 | Lenti-ORF clone of PHF8 (Myc-DDK-tagged)-Human PHD finger protein 8 (PHF8), transcript variant 4 |
CNY 10,640.00 |
|
| RC230556L4 | Lenti-ORF clone of PHF8 (mGFP-tagged)-Human PHD finger protein 8 (PHF8), transcript variant 4 |
CNY 10,640.00 |
|
| RG230556 | PHF8 (tGFP-tagged) - Human PHD finger protein 8 (PHF8), transcript variant 4 |
CNY 9,600.00 |
|
| SC329194 | PHF8 (untagged)-Human PHD finger protein 8 (PHF8) transcript variant 4 |
CNY 16,910.00 |
