GDA (NM_001242506) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CYPIN; GAH; GUANASE; NEDASIN
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC232618 representing NM_001242506 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTGGGCTGGTTGATACACACATCCATGCCTCTCAGTATTCCTTTGCTGGAAGTAGCATAGACCTGC CACTCTTGGAGTGGCTGACCAAGTACACATTTCCTGCAGAACACAGATTCCAGAACATCGACTTTGCAGA AGAAGTATATACCAGAGTTGTCAGGAGAACACTAAAGAATGGAACAACCACAGCTTGTTACTTTGCAACA ATTCACACTGACTCATCTCTGCTCCTTGCCGACATTACAGATAAATTTGGACAGCGGGCATTTGTGGGCA AAGTTTGCATGGATTTGAATGACACTTTTCCAGAATACAAGGAGACCACTGAGGAATCGATCAAGGAAAC TGAGAGATTTGTGTCAGAAATGCTCCAAAAGAACTATTCTAGAGTGAAGCCCATAGTGACACCACGTTTT TCCCTCTCCTGCTCTGAGACTTTGATGGGTGAACTGGGCAACATTGCTAAAACCCGTGATTTGCACATTC AGAGCCATATAAGTGAAAATCGTGATGAAGTTGAAGCTGTGAAAAACTTATACCCCAGTTATAAAAACTA CACATCTGTGTATGATAAAAACAATCTTTTGACAAATAAGACAGTGATGGCACACGGCTGCTACCTCTCT GCAGAAGAACTGAACGTATTCCATGAACGAGGAGCATCCATCGCACACTGTCCCAATTCTAATTTATCGC TCAGCAGTGGATTTCTAAATGTGCTAGAAGTCCTGAAACATGAAGTCAAGATAGGGCTGGGTACAGACGT GGCTGGTGGCTATTCATATTCCATGCTTGATGCAATCAGAAGAGCAGTGATGGTTTCCAATATCCTTTTA ATTAATAAGGTAAATGAGAAAAGCCTCACCCTCAAAGAAGTCTTCAGACTAGCTACTCTTGGAGGAAGCC AAGCCCTGGGGCTGGATGGTGAGATTGGAAACTTTGAAGTGGGCAAGGAATTTGATGCCATCCTGATCAA CCCCAAAGCATCCGACTCTCCCATTGACCTGTTTTATGGGGACTTTTTTGGTGATATTTCTGAGGCTGTT ATCCAGAAGTTCCTCTATCTAGGAGATGATCGAAATATTGAAGAGGTTTATGTGGGCGGAAAGCAGGTGG TTCCGTTTTCCAGCTCAGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC232618 representing NM_001242506 Red=Cloning site Green=Tags(s) MPGLVDTHIHASQYSFAGSSIDLPLLEWLTKYTFPAEHRFQNIDFAEEVYTRVVRRTLKNGTTTACYFAT IHTDSSLLLADITDKFGQRAFVGKVCMDLNDTFPEYKETTEESIKETERFVSEMLQKNYSRVKPIVTPRF SLSCSETLMGELGNIAKTRDLHIQSHISENRDEVEAVKNLYPSYKNYTSVYDKNNLLTNKTVMAHGCYLS AEELNVFHERGASIAHCPNSNLSLSSGFLNVLEVLKHEVKIGLGTDVAGGYSYSMLDAIRRAVMVSNILL INKVNEKSLTLKEVFRLATLGGSQALGLDGEIGNFEVGKEFDAILINPKASDSPIDLFYGDFFGDISEAV IQKFLYLGDDRNIEEVYVGGKQVVPFSSSV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001242506
ORF Size	1140 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001242506.3
RefSeq Size	5518 bp
RefSeq ORF	1143 bp
Locus ID	9615
UniProt ID	Q9Y2T3
Protein Pathways	Metabolic pathways, Purine metabolism
MW	42.9 kDa
Gene Summary	This gene encodes an enzyme responsible for the hydrolytic deamination of guanine. Studies in rat ortholog suggest this gene plays a role in microtubule assembly. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

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