PACAP receptor (ADCYAP1R1) (NM_001199636) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	PAC1; PAC1R; PACAPR; PACAPRI
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC232856 representing NM_001199636 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGGTGTCGTGCACGTTTCCCTGGCTGCTCTCCTCCTGCTGCCTATGGCCCCTGCCATGCATTCTG ACTGCATCTTCAAGAAGGAGCAAGCCATGTGCCTGGAGAAGATCCAGAGGGCCAATGAGCTGATGGGCTT CAATGATTCCTCTCCAGGCTGTCCTGGGATGTGGGACAACATCACGTGTTGGAAGCCCGCCCATGTGGGT GAGATGGTCCTGGTCAGCTGCCCTGAGCTCTTCCGAATCTTCAACCCAGACCAAGTCTGGGAGACCGAAA CCATTGGAGAGTCTGATTTTGGTGACAGTAACTCCTTAGATCTCTCAGACATGGGAGTGGTGAGCCGGAA CTGCACGGAGGATGGCTGGTCGGAACCCTTCCCTCATTACTTTGATGCCTGTGGGTTTGATGAATATGAA TCTGAGACTGGGGACCAGGATTATTACTACCTGTCAGTGAAGGCCCTCTACACGGTTGGCTACAGCACAT CCCTCGTCACCCTCACCACTGCCATGGTCATCCTTTGTCGCTTCCGGAAGCTGCACTGCACACGCAACTT CATCCACATGAACCTGTTTGTGTCGTTCATGCTGAGGGCGATCTCCGTCTTCATCAAAGACTGGATTCTG TATGCGGAGCAGGACAGCAACCACTGCTTCATCTCCACTGTGGAATGTAAGGCCGTCATGGTTTTCTTCC ACTACTGTGTTGTGTCCAACTACTTCTGGCTGTTCATCGAGGGCCTGTACCTCTTCACTCTGCTGGTGGA GACCTTCTTCCCTGAAAGGAGATACTTCTACTGGTACACCATCATTGGCTGGGGGACCCCAACTGTGTGT GTGACAGTGTGGGCTACGCTGAGACTCTACTTTGATGACACAGGCTGCTGGGATATGAATGACAGCACAG CTCTGTGGTGGGTGATCAAAGGCCCTGTGGTTGGCTCTATCATGGTTAACTTTGTGCTTTTTATTGGCAT TATCGTCATCCTTGTGCAGAAACTTCAGTCTCCAGACATGGGAGGCAATGAGTCCAGCATCTACTTCTGC GTGCAGAAATGCTACTGCAAGCCACAGCGGGCTCAGCAGCACTCTTGCAAGATGTCAGAACTGTCCACCA TTACTCTGCGACTGGCCCGGTCCACCCTGCTGCTCATCCCACTATTCGGAATCCACTACACAGTATTTGC CTTCTCCCCAGAGAATGTCAGCAAAAGGGAAAGACTCGTGTTTGAGCTGGGGCTGGGCTCCTTCCAGGGC TTTGTGGTGGCTGTTCTCTACTGTTTTCTGAATGGTGAGGTACAAGCGGAGATCAAGCGAAAATGGCGAA GCTGGAAGGTGAACCGTTACTTCGCTGTGGACTTCAAGCACCGACACCCGTCTCTGGCCAGCAGTGGGGT GAATGGGGGCACCCAGCTCTCCATCCTGAGCAAGAGCAGCTCCCAAATCCGCATGTCTGGCCTCCCTGCT GACAATCTGGCCACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC232856 representing NM_001199636 Red=Cloning site Green=Tags(s) MAGVVHVSLAALLLLPMAPAMHSDCIFKKEQAMCLEKIQRANELMGFNDSSPGCPGMWDNITCWKPAHVG EMVLVSCPELFRIFNPDQVWETETIGESDFGDSNSLDLSDMGVVSRNCTEDGWSEPFPHYFDACGFDEYE SETGDQDYYYLSVKALYTVGYSTSLVTLTTAMVILCRFRKLHCTRNFIHMNLFVSFMLRAISVFIKDWIL YAEQDSNHCFISTVECKAVMVFFHYCVVSNYFWLFIEGLYLFTLLVETFFPERRYFYWYTIIGWGTPTVC VTVWATLRLYFDDTGCWDMNDSTALWWVIKGPVVGSIMVNFVLFIGIIVILVQKLQSPDMGGNESSIYFC VQKCYCKPQRAQQHSCKMSELSTITLRLARSTLLLIPLFGIHYTVFAFSPENVSKRERLVFELGLGSFQG FVVAVLYCFLNGEVQAEIKRKWRSWKVNRYFAVDFKHRHPSLASSGVNGGTQLSILSKSSSQIRMSGLPA DNLAT TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001199636
ORF Size	1485 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001199636.2
RefSeq Size	6590 bp
RefSeq ORF	1488 bp
Locus ID	117
UniProt ID	P41586
Protein Families	Druggable Genome, GPCR, Transmembrane
Protein Pathways	Neuroactive ligand-receptor interaction
MW	56.9 kDa
Gene Summary	This gene encodes type I adenylate cyclase activating polypeptide receptor, which is a membrane-associated protein and shares significant homology with members of the glucagon/secretin receptor family. This receptor mediates diverse biological actions of adenylate cyclase activating polypeptide 1 and is positively coupled to adenylate cyclase. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Dec 2010]

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