Beta TRCP (BTRC) (NM_001256856) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	BETA-TRCP; betaTrCP; bTrCP; bTrCP1; FBW1A; FBXW1; FBXW1A; FWD1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC232989 representing NM_001256856 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCCGGCCGAGGCGGTGCTGCAAGAGAAGGCACTCAAGTTTATGTGCTCTATGCCCAGGTCTCTGT GGCTGGGCTGCTCCAGCCTGGCGGACAGCATGCCTTCGCTGCGATGCCTGTATAACCCAGGGACTGGCGC ACTCACAGCTTTCCAGACATACAACAGCTGTGCCAGACTCTGCTTAAACCAAGAAACAGTATGTTTAGCA AGCACTGCTATGAAGACTGAGAATTGTGTGGCCAAAACAAAACTTGCCAATGGCACTTCCAGTATGATTG TGCCCAAGCAACGGAAACTCTCAGCAAGCTATGAAAAGGAAAAGGAACTGTGTGTCAAATACTTTGAGCA GTGGTCAGAGTCAGATCAAGTGGAATTTGTGGAACATCTTATATCCCAAATGTGTCATTACCAACATGGG CACATAAACTCGTATCTTAAACCTATGTTGCAGAGAGATTTCATAACTGCTCTGCCAGCTCGGGGATTGG ATCATATTGCTGAGAACATTCTGTCATACCTGGATGCCAAATCACTATGTGCTGCTGAACTTGTGTGCAA GGAATGGTACCGAGTGACCTCTGATGGCATGCTGTGGAAGAAGCTTATCGAGAGAATGGTCAGGACAGAT TCTCTGTGGAGAGGCCTGGCAGAACGAAGAGGATGGGGACAGTATTTATTCAAAAACAAACCTCCTGACG GGAATGCTCCTCCCAACTCTTTTTATAGAGCACTTTATCCTAAAATTATACAAGACATTGAGACAATAGA ATCTAATTGGAGATGTGGAAGACATAGTTTACAGAGAATTCACTGCCGAAGTGAAACAAGCAAAGGAGTT TACTGTTTACAGTATGATGATCAGAAAATAGTAAGCGGCCTTCGAGACAACACAATCAAGATCTGGGATA AAAACACATTGGAATGCAAGCGAATTCTCACAGGCCATACAGGTTCAGTCCTCTGTCTCCAGTATGATGA GAGAGTGATCATAACAGGATCATCGGATTCCACGGTCAGAGTGTGGGATGTAAATACAGGTGAAATGCTA AACACGTTGATTCACCATTGTGAAGCAGTTCTGCACTTGCGTTTCAATAATGGCATGATGGTGACCTGCT CCAAAGATCGTTCCATTGCTGTATGGGATATGGCCTCCCCAACTGACATTACCCTCCGGAGGGTGCTGGT CGGACACCGAGCTGCTGTCAATGTTGTAGACTTTGATGACAAGTACATTGTTTCTGCATCTGGGGATAGA ACTATAAAGGTATGGAACACAAGTACTTGTGAATTTGTAAGGACCTTAAATGGACACAAACGAGGCATTG CCTGTTTGCAGTACAGGGACAGGCTGGTAGTGAGTGGCTCATCTGACAACACTATCAGATTATGGGACAT AGAATGTGGTGCATGTTTACGAGTGTTAGAAGGCCATGAGGAATTGGTGCGTTGTATTCGATTTGATAAC AAGAGGATAGTCAGTGGGGCCTATGATGGAAAAATTAAAGTGTGGGATCTTGTGGCTGCTTTGGACCCCC GTGCTCCTGCAGGGACACTCTGTCTACGGACCCTTGTGGAGCATTCCGGAAGAGTTTTTCGACTACAGTT TGATGAATTCCAGATTGTCAGTAGTTCACATGATGACACAATCCTCATCTGGGACTTCCTAAATGATCCA GCTGCCCAAGCTGAACCCCCCCGTTCCCCTTCTCGAACATACACCTACATCTCCAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC232989 representing NM_001256856 Red=Cloning site Green=Tags(s) MDPAEAVLQEKALKFMCSMPRSLWLGCSSLADSMPSLRCLYNPGTGALTAFQTYNSCARLCLNQETVCLA STAMKTENCVAKTKLANGTSSMIVPKQRKLSASYEKEKELCVKYFEQWSESDQVEFVEHLISQMCHYQHG HINSYLKPMLQRDFITALPARGLDHIAENILSYLDAKSLCAAELVCKEWYRVTSDGMLWKKLIERMVRTD SLWRGLAERRGWGQYLFKNKPPDGNAPPNSFYRALYPKIIQDIETIESNWRCGRHSLQRIHCRSETSKGV YCLQYDDQKIVSGLRDNTIKIWDKNTLECKRILTGHTGSVLCLQYDERVIITGSSDSTVRVWDVNTGEML NTLIHHCEAVLHLRFNNGMMVTCSKDRSIAVWDMASPTDITLRRVLVGHRAAVNVVDFDDKYIVSASGDR TIKVWNTSTCEFVRTLNGHKRGIACLQYRDRLVVSGSSDNTIRLWDIECGACLRVLEGHEELVRCIRFDN KRIVSGAYDGKIKVWDLVAALDPRAPAGTLCLRTLVEHSGRVFRLQFDEFQIVSSSHDDTILIWDFLNDP AAQAEPPRSPSRTYTYISR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001256856
ORF Size	1737 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001256856.1, NP_001243785.1
RefSeq Size	6102 bp
RefSeq ORF	1740 bp
Locus ID	8945
Protein Families	Druggable Genome
Protein Pathways	Hedgehog signaling pathway, Oocyte meiosis, Ubiquitin mediated proteolysis, Wnt signaling pathway
MW	66.3 kDa
Gene Summary	This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. The encoded protein mediates degradation of CD4 via its interaction with HIV-1 Vpu. It has also been shown to ubiquitinate phosphorylated NFKBIA (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha), targeting it for degradation and thus activating nuclear factor kappa-B. Alternatively spliced transcript variants have been described. A related pseudogene exists in chromosome 6. [provided by RefSeq, Mar 2012]

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