DGKH (NM_001204506) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	DGKeta
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC233198 representing NM_001204506 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGTGTGCTGAGAACAGAAAGGAGATGGAGGATTGGATCAGCTCACTGAAGTCTGTACAGACCAGAG AACCCTACGAGGTGGCCCAGTTTAATGTGGAACATTTCTCAGGGATGCACAACTGGTACGCCTGCTCCCA CGCCCGACCCACCTTCTGTAACGTGTGCAGAGAGAGTCTTTCTGGAGTCACCTCCCATGGCCTGTCCTGC GAAGTGTGTAAATTCAAGGCTCACAAAAGATGTGCAGTGAGAGCAACAAATAACTGTAAATGGACTACCC TGGCCTCCATCGGGAAGGACATTATAGAAGATGAAGATGGCGTCGCGATGCCTCACCAGTGGCTTGAGGG CAACCTGCCTGTAAGTGCCAAGTGTGCTGTCTGCGACAAAACATGTGGCAGTGTTCTCCGTCTACAGGAT TGGAAATGCCTTTGGTGTAAGACAATGGTACACACTGCCTGCAAAGATTTATACCATCCAATATGTCCAC TTGGTCAATGTAAAGTATCTATCATACCTCCAATTGCACTAAACAGCACCGATTCCGATGGTTTCTGTAG AGCAACATTTTCGTTCTGTGTTAGTCCTCTATTGGTTTTTGTCAATTCTAAGAGTGGAGATAATCAGGGA GTAAAGTTCCTCCGTCGCTTTAAACAGTTGCTAAATCCGGCTCAGGTGTTTGATTTAATGAATGGAGGTC CTCATTTAGGTTTAAGATTATTTCAGAAGTTTGACAATTTCCGGATTCTTGTTTGTGGAGGCGATGGAAG TGTAGGTTGGGTTTTGTCAGAAATCGATAAGCTCAACTTGAATAAACAGTGTCAGCTGGGAGTGTTGCCT TTGGGTACAGGAAATGACCTTGCCCGAGTTCTTGGCTGGGGAGGTTCATATGACGATGACACCCAACTTC CTCAGATCCTAGAGAAACTGGAACGAGCCAGTACCAAAATGTTGGACAGGTGGAGTATAATGACATATGA ACTCAAATTGCCACCAAAAGCTTCCCTACTTCCAGGACCTCCAGAAGCATCTGAAGAATTTTATATGACG ATTTATGAAGACTCAGTTGCAACGCATCTTACAAAAATCCTCAATTCTGATGAACATGCAGTGGTCATAT CTTCTGCCAAGACGCTATGTGAAACTGTAAAGGACTTCGTTGCCAAAGTAGAAAAGACGTATGACAAAAC CTTGGAAAATGCCGTTGTAGCTGATGCCGTGGCCAGTAAATGTTCAGTCCTAAACGAGAAGCTCGAACAA CTGCTGCAGGCTTTGCACACAGATTCCCAGGCTGCGCCTGTTCTCCCTGGCCTCAGCCCTCTCATTGTGG AAGAAGATGCTGTGGAATCGTCCAGTGAAGAGTCCCTGGGTGAAAGCAAAGAGCAGCTTGGGGATGACGT TACAAAACCTTCCTCCCAGAAAGCCGTCAAACCAAGGGAAATCATGTTGCGGGCAAATAGTTTAAAGAAA GCAGTGAGGCAAGTCATTGAGGAAGCCGGAAAAGTTATGGATGACCCGACAGTTCACCCCTGTGAACCAG CTAATCAGTCCTCTGATTATGACAGCACAGAAACAGATGAATCTAAGGAGGAAGCTAAAGATGATGGTGC CAAAGAATCAATAACTGTTAAAACTGCACCTCGGTCTCCAGATGCCCGGGCAAGTTATGGCCATTCCCAA ACTGATTCTGTCCCTGGTCCAGCTGTGGCAGCCAGCAAAGAAAACCTCCCTGTGCTCAATACCAGAATAA TCTGCCCAGGTTTAAGAGCAGGACTGGCTGCCTCAATTGCTGGGAGTTCGATTATCAACAAAATGTTACT GGCAAACATTGATCCTTTTGGTGCCACGCCGTTTATTGACCCGGATCTAGATTCCGTAGATGGATATTCA GAAAAATGTGTCATGAACAATTACTTTGGGATTGGATTAGATGCAAAAATTTCATTAGAATTTAATAATA AAAGAGAGGAGCACCCTGAAAAATGCAGGAGCCGAACTAAAAACTTGATGTGGTATGGAGTCCTTGGAAC CCGGGAGTTATTACAGAGATCGTACAAGAATTTAGAACAAAGGGTTCAACTTGAGTGTGATGGGCAGTAT ATTCCTCTTCCCAGCTTGCAAGGCATAGCCGTGTTGAACATTCCCAGCTATGCTGGAGGCACTAACTTTT GGGGTGGAACTAAAGAGGATGATATATTTGCTGCACCATCCTTTGATGACAAGATCCTGGAAGTTGTAGC AATATTTGATAGCATGCAAATGGCAGTTTCAAGGGTCATTAAACTGCAGCATCATCGAATAGCCCAGTGC CGTACAGTGAAAATCACTATATTTGGTGACGAAGGAGTCCCAGTGCAAGTGGATGGTGAAGCGTGGGTTC AGCCTCCAGGGATTATCAAAATTGTGCACAAAAACAGAGCACAAATGCTAACAAGGGACAGAGCCTTTGA GAGCACTCTGAAATCTTGGGAAGATAAGCAGAAGTGTGATTCTGGTAAACCAGTTCTCCGAACCCATTTG TACATCCATCACGCCATTGACTTGGCAACAGAAGAGGTGTCGCAGATGCAGCTATGCTCCCAGGCTGCAG AGGAGCTCATTACTAGGATATGTGACGCAGCCACAATTCACTGTCTTTTGGAGCAAGAACTGGCCCATGC TGTGAATGCCTGCTCCCATGCCCTGAATAAAGCCAACCCAAGGTGCCCGGAGAGTCTTACAAGAGACACT GCCACTGAAATAGCCATCAATGTGAAGGCGCTGTATAATGAAACAGAATCTTTGCTAGTTGGCAGGGTTC CTTTGCAGCTGGAATCGCCACATGAAGAGCGAGTATCCAATGCCTTACACTCTGTGGAGGTGGAATTACA GAAACTGACAGAGATTCCTTGGCTTTATTATATCTTACACCCAAATGAGGATGAGGAACCTCCTATGGAT TGCACCAAAAGGAACAACAGAAGCACCGTATTTCGAATAGTGCCAAAGTTTAAAAAGGAAAAGGTTCAGA AGCAGAAGACAAGTTCACAGCCTGTTCAGAAATGGGGCACAGAGGAAGTTGCTGCTTGGCTGGATCTGCT CAATTTGGGAGAGTACAAAGATATCTTCATCCGTCATGACATCAGAGGGGCTGAACTTTTGCATCTGGAA AGGCGAGATCTTAAGGATCTGGGGATACCGAAAGTGGGTCATGTGAAGCGAATTCTCCAGGGAATTAAAG AGCTTGGAAGGAGCACTCCACAGTCGGAGGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC233198 representing NM_001204506 Red=Cloning site Green=Tags(s) MLCAENRKEMEDWISSLKSVQTREPYEVAQFNVEHFSGMHNWYACSHARPTFCNVCRESLSGVTSHGLSC EVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDEDGVAMPHQWLEGNLPVSAKCAVCDKTCGSVLRLQD WKCLWCKTMVHTACKDLYHPICPLGQCKVSIIPPIALNSTDSDGFCRATFSFCVSPLLVFVNSKSGDNQG VKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDNFRILVCGGDGSVGWVLSEIDKLNLNKQCQLGVLP LGTGNDLARVLGWGGSYDDDTQLPQILEKLERASTKMLDRWSIMTYELKLPPKASLLPGPPEASEEFYMT IYEDSVATHLTKILNSDEHAVVISSAKTLCETVKDFVAKVEKTYDKTLENAVVADAVASKCSVLNEKLEQ LLQALHTDSQAAPVLPGLSPLIVEEDAVESSSEESLGESKEQLGDDVTKPSSQKAVKPREIMLRANSLKK AVRQVIEEAGKVMDDPTVHPCEPANQSSDYDSTETDESKEEAKDDGAKESITVKTAPRSPDARASYGHSQ TDSVPGPAVAASKENLPVLNTRIICPGLRAGLAASIAGSSIINKMLLANIDPFGATPFIDPDLDSVDGYS EKCVMNNYFGIGLDAKISLEFNNKREEHPEKCRSRTKNLMWYGVLGTRELLQRSYKNLEQRVQLECDGQY IPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDIFAAPSFDDKILEVVAIFDSMQMAVSRVIKLQHHRIAQC RTVKITIFGDEGVPVQVDGEAWVQPPGIIKIVHKNRAQMLTRDRAFESTLKSWEDKQKCDSGKPVLRTHL YIHHAIDLATEEVSQMQLCSQAAEELITRICDAATIHCLLEQELAHAVNACSHALNKANPRCPESLTRDT ATEIAINVKALYNETESLLVGRVPLQLESPHEERVSNALHSVEVELQKLTEIPWLYYILHPNEDEEPPMD CTKRNNRSTVFRIVPKFKKEKVQKQKTSSQPVQKWGTEEVAAWLDLLNLGEYKDIFIRHDIRGAELLHLE RRDLKDLGIPKVGHVKRILQGIKELGRSTPQSEV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001204506
ORF Size	3252 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001204506.3
RefSeq Size	3956 bp
RefSeq ORF	3255 bp
Locus ID	160851
UniProt ID	Q86XP1
Protein Families	Druggable Genome
Protein Pathways	Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Phosphatidylinositol signaling system
MW	121 kDa
Gene Summary	This gene encodes a member of the diacylglycerol kinase (DGK) enzyme family. Members of this family are involved in regulating intracellular concentrations of diacylglycerol and phosphatidic acid. Variation in this gene has been associated with bipolar disorder. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Jul 2014]

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