Ensconsin (MAP7) (NM_001198615) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	E-MAP-115; EMAP115
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234794 representing NM_001198615 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGACACTAAACTGTACTCACCCGACAGCTACAAAGTGCAAGATAAGAAAAATGCCTCCAGCCGCC CTGCCTCTGCAATTTCAGGACAAAATAACAACCACTCAGGAAATAAACCAGACCCTCCGCCTGTGTTACG TGTTGATGACCGGCAGCGGCTGGCCCGGGAGCGACGTGAGGAACGGGAGAAACAGCTAGCTGCAAGAGAA ATAGTGTGGTTAGAAAGAGAAGAGCGAGCCAGGCAGCACTACGAGAAGCACCTGGAAGAGCGGAAGAAGA GGTTGGAGGAGCAGAGGCAGAAGGAGGAGCGGAGGAGGGCTGCTGTGGAGGAGAAGCGGAGGCAGAGACT TGAGGAGGACAAAGAACGCCACGAAGCTGTTGTACGGCGCACAATGGAAAGGAGCCAGAAGCCAAAACAG AAGCATAACCGTTGGTCGTGGGGAGGCTCTCTCCATGGGAGCCCTAGCATCCACAGTGCAGATCCAGACA GGCGGTCAGTTTCCACCATGAATCTTTCGAAATATGTTGATCCCGTCATTAGCAAGCGGCTCTCCTCTTC ATCTGCAACTTTACTAAATTCTCCAGATAGAGCTCGCCGCCTGCAGCTCAGCCCATGGGAGAGCAGCGTT GTTAACAGACTCCTGACGCCCACACATTCGTTCCTGGCCAGAAGTAAAAGCACAGCTGCCTTGTCTGGAG AAGCAGCATCTTGCAGCCCCATCATCATGCCCTACAAAGCTGCACACTCTAGAAATTCGATGGATCGACC AAAACTCTTTGTAACACCACCTGAGGGCTCTTCTCGCAGGAGGATCATTCATGGCACAGCGAGCTATAAA AAAGAAAGAGAGAGAGAAAATGTACTCTTCCTCACATCTGGCACCCGAAGGGCTGTATCTCCATCTAATC CCAAAGCAAGACAACCAGCTCGCTCCCGACTTTGGCTTCCGTCCAAGTCTCTTCCTCATTTGCCTGGCAC ACCCAGACCGACATCCTCCTTGCCACCCGGCTCAGTCAAAGCTGCTCCTGCTCAGGTCCGGCCCCCATCC CCCGGCAACATCCGCCCTGTCAAGAGGGAAGTCAAAGTGGAGCCTGAGAAGAAAGATCCTGAGAAGGAAC CTCAGAAAGTTGCCAATGAGCCCTCACTAAAGGGCAGAGCACCTTTAGTGAAGGTAGAAGAAGCCACAGT TGAAGAGCGGACACCTGCTGAACCAGAAGTTGGCCCTGCTGCTCCAGCCATGGCCCCAGCTCCAGCCTCG GCCCCAGCTCCAGCCTCGGCCCCAGCTCCAGCCCCGGTCCCCACCCCAGCCATGGTCTCAGCCCCGTCAT CCACTGTGAATGCCAGTGCTTCTGTTAAGACTTCTGCAGGCACCACCGACCCAGAGGAGGCCACAAGGCT TCTAGCTGAGAAGAGGCGGCTGGCCCGAGAGCAGAGAGAAAAGGAAGAAAGGGAGAGGAGGGAGCAGGAA GAGCTTGAAAGACAAAAGAGAGAGGAATTGGCTCAACGTGTGGCTGAAGAGAGGACGACTCGCCGTGAGG AGGAGTCGCGCAGGCTGGAAGCCGAGCAGGCCCGGGAGAAGGAGGAGCAGCTGCAGCGGCAGGCGGAGGA GCGGGCGCTGCGCGAGCGGGAGGAGGCAGAGCGCGCCCAGAGGCAGAAAGAAGAAGAAGCTCGCGTTCGT GAAGAAGCAGAGAGGGTCCGGCAGGAACGAGAGAAGCATTTCCAGAGAGAAGAGCAAGAGCGCCTGGAGA GAAAGAAGCGACTTGAGGAGATTATGAAAAGAACCAGGAGAACAGAAGCTACAGATAAGAAAACCAGTGA TCAGAGAAACGGTGATATAGCCAAGGGAGCTCTCACTGGAGGAACAGAGGTGTCTGCACTTCCATGTACA ACAAACGCTCCGGGAAATGGAAAGCCAGTTGGCAGCCCACATGTGGTTACCTCACACCAGTCAAAAGTGA CAGTGGAGAGCACTCCCGATTTGGAAAAACAACCAAATGAAAATGGTGTATCTGTTCAGAATGAAAATTT TGAAGAAATTATAAACTTACCCATTGGATCTAAACCATCCAGATTAGATGTCACCAACAGTGAGAGCCCA GAAATTCCTTTGAATCCAATTTTGGCCTTTGATGATGAAGGGACACTTGGGCCCCTGCCTCAGGTAGATG GTGTTCAGACACAGCAGACTGCAGAAGTTATA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234794 representing NM_001198615 Red=Cloning site Green=Tags(s) MEDTKLYSPDSYKVQDKKNASSRPASAISGQNNNHSGNKPDPPPVLRVDDRQRLARERREEREKQLAARE IVWLEREERARQHYEKHLEERKKRLEEQRQKEERRRAAVEEKRRQRLEEDKERHEAVVRRTMERSQKPKQ KHNRWSWGGSLHGSPSIHSADPDRRSVSTMNLSKYVDPVISKRLSSSSATLLNSPDRARRLQLSPWESSV VNRLLTPTHSFLARSKSTAALSGEAASCSPIIMPYKAAHSRNSMDRPKLFVTPPEGSSRRRIIHGTASYK KERERENVLFLTSGTRRAVSPSNPKARQPARSRLWLPSKSLPHLPGTPRPTSSLPPGSVKAAPAQVRPPS PGNIRPVKREVKVEPEKKDPEKEPQKVANEPSLKGRAPLVKVEEATVEERTPAEPEVGPAAPAMAPAPAS APAPASAPAPAPVPTPAMVSAPSSTVNASASVKTSAGTTDPEEATRLLAEKRRLAREQREKEERERREQE ELERQKREELAQRVAEERTTRREEESRRLEAEQAREKEEQLQRQAEERALREREEAERAQRQKEEEARVR EEAERVRQEREKHFQREEQERLERKKRLEEIMKRTRRTEATDKKTSDQRNGDIAKGALTGGTEVSALPCT TNAPGNGKPVGSPHVVTSHQSKVTVESTPDLEKQPNENGVSVQNENFEEIINLPIGSKPSRLDVTNSESP EIPLNPILAFDDEGTLGPLPQVDGVQTQQTAEVI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001198615
ORF Size	2202 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001198615.1, NP_001185544.1
RefSeq Size	4245 bp
RefSeq ORF	2205 bp
Locus ID	9053
UniProt ID	Q14244
Protein Families	Druggable Genome
MW	83.3 kDa
Gene Summary	The product of this gene is a microtubule-associated protein that is predominantly expressed in cells of epithelial origin. Microtubule-associated proteins are thought to be involved in microtubule dynamics, which is essential for cell polarization and differentiation. This protein has been shown to be able to stabilize microtubules, and may serve to modulate microtubule functions. Studies of the related mouse protein also suggested an essential role in microtubule function required for spermatogenesis. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]

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