NOP2 (NM_001258308) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	NOL1; NOP120; NSUN1; p120
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234920 representing NM_001258308 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCGCAAGTTGGACCCTACGAAGGAGAAGCGGGGGCCAGGCCGAAAGGCCCGGAAGCAGAAGGGTG CCGAGACAGAACTCGTCAGATTCTTGCCTGCAGTAAGTGACGAAAATTCCAAGAGGCTGTCTAGTCGTGC TCGAAAGAGGGCAGCCAAGAGGAGATTGGGCTCTGTTGAAGCCCCTAAGACAAATAAGTCTCCTGAGGCC AAACCATTGCCTGGAAAGCTACCAAAAGGGATCTCTGCAGGAGCTGTCCAGACAGCTGGTAAGAAGGGAC CCCAGTCCCTATTTAATGCTCCTCGAGGCAAGAAGCGCCCAGCACCTGGCAGTGATGAGGAAGAGGAGGA GGAAGACTCTGAAGAAGATGGTATGGTGAACCACGGGGACCTCTGGGGCTCCGAGGACGATGCTGATACG GTAGATGACTATGGAGCTGACTCCAACTCTGAGGATGAGGAGGAAGGTGAAGCGTTGCTGCCCATTGAAA GAGCTGCTCGGAAGCAGAAGGCCCGGGAAGCTGCTGCTGGGATCCAGTGGAGTGAAGAGGAGACCGAGGA CGAGGAGGAAGAGAAAGAAGTGACCCCTGAGTCAGGCCCCCCAAAGGTGGAAGAGGCAGATGGGGGCCTG CAGATCAATGTGGATGAGGAACCATTTGTGCTGCCCCCTGCTGGGGAGATGGAGCAGGATGCCCAGGCTC CAGACCTGCAACGAGTTCACAAGCGGATCCAGGATATTGTGGGAATTCTGCGTGATTTTGGGGCTCAGCG GGAGGAAGGGCGGTCTCGTTCTGAATACCTGAACCGGCTCAAGAAGGATCTGGCCATTTACTACTCCTAT GGAGACTTCCTGCTTGGCAAGCTCATGGACCTCTTCCCTCTGTCTGAGCTGGTGGAGTTCTTAGAAGCTA ATGAGGTGCCTCGGCCCGTCACCCTCCGGACCAATACCTTGAAAACCCGACGCCGAGACCTTGCACAGGC TCTAATCAATCGTGGGGTTAACCTGGATCCCCTGGGCAAGTGGTCAAAGACTGGACTAGTGGTGTATGAT TCTTCTGTGCCCATTGGTGCTACCCCCGAGTACCTGGCTGGGCACTACATGCTGCAGGGAGCCTCCAGCA TGTTGCCCGTCATGGCCTTGGCACCCCAGGAACATGAGCGGATCCTGGACATGTGTTGTGCCCCTGGAGG AAAGACCAGCTACATGGCCCAGCTGATGAAGAACACGGGTGTGATCCTTGCCAATGACGCCAATGCTGAG CGGCTCAAGAGTGTTGTGGGCAACTTGCATCGGCTGGGAGTCACCAACACCATTATCAGCCACTATGATG GGCGCCAGTTCCCCAAGGTGGTGGGGGGCTTTGACCGAGTACTGCTGGATGCTCCCTGCAGTGGCACTGG GGTCATCTCCAAGGATCCAGCCGTGAAGACTAACAAGGATGAGAAGGACATCCTGCGCTGTGCTCACCTC CAGAAGGAGTTGCTCCTGAGTGCTATTGACTCTGTCAATGCGACCTCCAAGACAGGAGGCTACCTGGTTT ACTGCACCTGTTCTATCACAGTAGAAGAGAATGAGTGGGTGGTAGACTATGCTCTGAAAAAGAGGAATGT GCGACTGGTGCCCACGGGCCTAGACTTTGGCCAGGAAGGTTTTACCCGCTTTCGAGAAAGGCGCTTCCAC CCCAGTCTGCGTTCTACCCGACGCTTCTACCCTCATACCCACAATATGGATGGGTTCTTCATTGCCAAGT TCAAGAAATTTTCCAATTCTATCCCTCAGTCCCAGACAGGAAATTCTGAAACAGCCACACCTACAAATGT AGACTTGCCTCAGGTCATCCCCAAGTCTGAGAACAGCAGCCAGCCAGCCAAGAAAGCCAAGGGGGCTGCA AAGACAAAGCAGCAGCTGCAGAAACAGCAACATCCCAAGAAGGCCTCCTTCCAGAAGCTGAATGGCATCT CCAAAGGGGCAGACTCAGAATTGTCCACTGTACCTTCTGTCACAAAGACCCAAGCTTCCTCCAGCTTCCA GGATAGCAGTCAGCCAGCTGGAAAAGCCGAAGGGATCAGGGAGCCAAAGGTGACTGGGAAGCTAAAGCAA CGATCACCTAAATTACAGTCCTCCAAGAAAGTTGCTTTCCTCAGGCAGAATGCCCCTCCCAAGGGCACAG ACACACAAACACCGGCTGTGTTATCCCCATCCAAGACTCAGGCCACCCTGAAACCTAAGGACCATCATCA GCCCCTTGGAAGGGCCAAGGGGGTTGAGAAGCAGCAGTTGCCAGAGCAGCCTTTTGAGAAAGCTGCCTTC CAGAAACAGAATGATACCCCCAAGGGGCCTCAGCCTCCCACTGTGTCTCCCATCCGTTCCAGCCGCCCCC CACCAGCAAAGAGGAAGAAATCTCAGTCCAGGGGCAACAGCCAGCTGCTGCTATCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234920 representing NM_001258308 Red=Cloning site Green=Tags(s) MGRKLDPTKEKRGPGRKARKQKGAETELVRFLPAVSDENSKRLSSRARKRAAKRRLGSVEAPKTNKSPEA KPLPGKLPKGISAGAVQTAGKKGPQSLFNAPRGKKRPAPGSDEEEEEEDSEEDGMVNHGDLWGSEDDADT VDDYGADSNSEDEEEGEALLPIERAARKQKAREAAAGIQWSEEETEDEEEEKEVTPESGPPKVEEADGGL QINVDEEPFVLPPAGEMEQDAQAPDLQRVHKRIQDIVGILRDFGAQREEGRSRSEYLNRLKKDLAIYYSY GDFLLGKLMDLFPLSELVEFLEANEVPRPVTLRTNTLKTRRRDLAQALINRGVNLDPLGKWSKTGLVVYD SSVPIGATPEYLAGHYMLQGASSMLPVMALAPQEHERILDMCCAPGGKTSYMAQLMKNTGVILANDANAE RLKSVVGNLHRLGVTNTIISHYDGRQFPKVVGGFDRVLLDAPCSGTGVISKDPAVKTNKDEKDILRCAHL QKELLLSAIDSVNATSKTGGYLVYCTCSITVEENEWVVDYALKKRNVRLVPTGLDFGQEGFTRFRERRFH PSLRSTRRFYPHTHNMDGFFIAKFKKFSNSIPQSQTGNSETATPTNVDLPQVIPKSENSSQPAKKAKGAA KTKQQLQKQQHPKKASFQKLNGISKGADSELSTVPSVTKTQASSSFQDSSQPAGKAEGIREPKVTGKLKQ RSPKLQSSKKVAFLRQNAPPKGTDTQTPAVLSPSKTQATLKPKDHHQPLGRAKGVEKQQLPEQPFEKAAF QKQNDTPKGPQPPTVSPIRSSRPPPAKRKKSQSRGNSQLLLS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001258308
ORF Size	2436 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001258308.2
RefSeq Size	2716 bp
RefSeq ORF	2439 bp
Locus ID	4839
UniProt ID	P46087
MW	89.8 kDa
Gene Summary	Involved in ribosomal large subunit assembly (PubMed:24120868). S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C(5) position of cytosine 4447 in 28S rRNA (Probable). May play a role in the regulation of the cell cycle and the increased nucleolar activity that is associated with the cell proliferation (Probable).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.