DDX11 (NM_001257145) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CHL1; CHLR1; KRG2; WABS
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234990 representing NM_001257145 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGAGCTGTACCGGGTTTTGGAGGCTGGCAAGATTGGGATATTTGAGAGTCCAACTGGCACTGGGA AGTCCTTAAGTCTTATTTGTGGGGCCCTCTCTTGGCTCCGTGACTTTGAACAGAAGAAGCGTGAAGAAGA GGCACGACTCCTTGAAACTGGAACTGGCCCCTTACATGATGAGAAAGATGAATCCCTGTGTCTGTCTTCT TCCTGCGAAGGGGCTGCAGGCACCCCGAGGCCTGCTGGAGAACCGGCCTGGGTTACTCAGTTTGTGCAGA AGAAAGAAGAGAGGGACCTGGTGGACCGACTAAAGGCGGAGCAGGCCAGGAGGAAGCAGCGAGAAGAACG CCTGCAGCAGCTGCAGCACAGGGTGCAGCTCAAGTATGCAGCCAAGCGCCTGAGGCAGGAAGAAGAAGAA AGAGAGAATCTCCTCCGCCTCAGCAGGGAGATGCTAGAGACAGGCCCGGAGGCTGAGCGGCTGGAGCAGC TGGAGTCTGGGGAGGAGGAGCTGGTCCTCGCCGAATACGAGAGTGATGAGGAGAAAAAGGTGGCGAGCAG AGTGGATGAGGATGAGGATGACCTGGAGGAAGAACACATAACTAAGATTTATTACTGTAGTCGGACACAC TCCCAGCTGGCCCAGTTTGTGCATGAGGTGAAGAAGAGCCCCTTTGGCAAGGATGTTCGGCTGGTCTCCC TTGGCTCCCGGCAGAACCTTTGTGTAAATGAAGACGTGAAAAGCCTAGGTTCTGTGCAGCTTATCAACGA CCGCTGTGTGGACATGCAGAGAAGCAGGCACGAGAAGAAGAAAGGAGCTGAGGAGGAGAAGCCAAAGAGG AGGAGGCAGGAGAAGCAGGCAGCCTGCCCCTTCTACAACCACGAGCAGATGGGCCTTCTCCGGGATGAGG CCCTGGCAGAGGTGAAGGACATGGAGCAGCTGCTGGCCCTTGGGAAGGAGGCCCGGGCCTGTCCCTATTA CGGGAGCCGCCTTGCCATCCCTGCAGCCCAGCTGGTGGTGCTGCCCTATCAGATGCTGCTGCATGCGGCC ACTCGGCAGGCCGCGGGCATCCGGCTGCAGGACCAGGTGGTGATCATCGACGAGGCGCACAACCTGATCG ACACCATCACGGGCATGCACAGCGTGGAGGTCAGCGGCTCCCAGCTCTGCCAGGCCCATTCCCAGCTGCT GCAGTACGTGGAGCGATACGGGAAGCGTTTGAAGGCCAAGAACCTGATGTACCTGAAGCAGATCCTGTAT TTGCTGGAGAAATTCGTGGCTGTGCTAGGGGGGAACATTAAGCAAAATCCCAATACACAGAGTCTGTCAC AGACAGGGACGGAGCTGAAGACCATCAACGACTTTCTCTTCCAGAGCCAGATCGACAACATCAACCTGTT CAAGGTGCAGCGATACTGTGAGAAGAGCATGATCAGCAGAAAGCTCTTTGGATTCACTGAACGGTACGGA GCAGTGTTCTCATCCCGGGAGCAGCCCAAACTGGCTGGGTTTCAGCAATTCCTGCAGAGCCTGCAGCCCA GGACGACTGAAGCTCTTGCAGCCCCTGCAGACGAGAGTCAGGCCAGCACCCTGCGACCAGCTTCTCCACT GATGCACATCCAAGGCTTCCTGGCAGCTCTCACTACGGCCAACCAGGACGGCAGGGTCATCCTGAGCCGC CAAGGCAGCCTCAGTCAGAGCACCCTGAAGTTTTTGCTCCTGAATCCAGCTGTGCACTTTGCCCAAGTGG TGAAGGAATGCCGGGCAGTGGTCATTGCGGGGGGTACCATGCAGCCGGTGTCTGACTTCCGGCAGCAGCT GCTGGCCTGTGCCGGGGTGGAAGCTGAGCGCGTGGTGGAGTTTTCCTGTGGTCACGTGATCCCTCCAGAC AACATCCTGCCCCTCGTCATCTGCAGCGGGATCTCCAACCAGCCGCTGGAATTCACGTTCCAGAAAAGAG AGCTGCCTCAGATGATGGACGAGGTGGGTCGCATTCTCTGTAACCTGTGCGGTGTGGTTCCTGGAGGGGT GGTCTGTTTCTTCCCCTCCTACGAGTACCTGCGCCAGGTCCATGCCCACTGGGAGAAGGGTGGCCTGCTG GGCCGTCTGGCTGCCAGGAAGAAGATATTCCAGGAACCTAAGAGCGCACACCAGGTGGAGCAGGTGCTGC TGGCATATTCCAGGTGCATCCAGGCCTGTGGCCAGGAGAGAGGCCAGGTGACAGGGGCCCTGCTCCTCTC TGTGGTTGGAGGAAAGATGAGTGAAGGGATCAACTTCTCTGACAACCTAGGCCGGTGTGTGGTGATGGTG GGCATGCCCTTCCCCAACATCAGGTCTGCAGAGCTGCAGGAGAAGATGGCCTACTTGGATCAAACCCTCC CCAGAGCCCCCGGCCAGGCACCCCCAGGGAAGGCTCTGGTGGAGAACCTGTGCATGAAGGCCGTCAACCA GTCCATAGGCAGGGCCATCAGGCACCAGAAGGATTTTGCCAGCGTAGTGCTCCTGGACCAGCGATATGCC CGGCCCCCTGTCCTGGCCAAGCTGCCGGCCTGGATCCGAGCCCGTGTGGAGGTCAAAGCTACCTTTGGCC CCGCCATTGCTGCTGTGCAGAAGTTTCACCGGGAGAAGTCGGCCTCTTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234990 representing NM_001257145 Red=Cloning site Green=Tags(s) MAELYRVLEAGKIGIFESPTGTGKSLSLICGALSWLRDFEQKKREEEARLLETGTGPLHDEKDESLCLSS SCEGAAGTPRPAGEPAWVTQFVQKKEERDLVDRLKAEQARRKQREERLQQLQHRVQLKYAAKRLRQEEEE RENLLRLSREMLETGPEAERLEQLESGEEELVLAEYESDEEKKVASRVDEDEDDLEEEHITKIYYCSRTH SQLAQFVHEVKKSPFGKDVRLVSLGSRQNLCVNEDVKSLGSVQLINDRCVDMQRSRHEKKKGAEEEKPKR RRQEKQAACPFYNHEQMGLLRDEALAEVKDMEQLLALGKEARACPYYGSRLAIPAAQLVVLPYQMLLHAA TRQAAGIRLQDQVVIIDEAHNLIDTITGMHSVEVSGSQLCQAHSQLLQYVERYGKRLKAKNLMYLKQILY LLEKFVAVLGGNIKQNPNTQSLSQTGTELKTINDFLFQSQIDNINLFKVQRYCEKSMISRKLFGFTERYG AVFSSREQPKLAGFQQFLQSLQPRTTEALAAPADESQASTLRPASPLMHIQGFLAALTTANQDGRVILSR QGSLSQSTLKFLLLNPAVHFAQVVKECRAVVIAGGTMQPVSDFRQQLLACAGVEAERVVEFSCGHVIPPD NILPLVICSGISNQPLEFTFQKRELPQMMDEVGRILCNLCGVVPGGVVCFFPSYEYLRQVHAHWEKGGLL GRLAARKKIFQEPKSAHQVEQVLLAYSRCIQACGQERGQVTGALLLSVVGGKMSEGINFSDNLGRCVVMV GMPFPNIRSAELQEKMAYLDQTLPRAPGQAPPGKALVENLCMKAVNQSIGRAIRHQKDFASVVLLDQRYA RPPVLAKLPAWIRARVEVKATFGPAIAAVQKFHREKSASS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001257145
ORF Size	2640 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001257145.2
RefSeq Size	3737 bp
RefSeq ORF	2643 bp
Locus ID	1663
UniProt ID	Q96FC9
Protein Families	Stem cell - Pluripotency
MW	99.1 kDa
Gene Summary	DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an enzyme that possesses both ATPase and DNA helicase activities. This gene is a homolog of the yeast CHL1 gene, and may function to maintain chromosome transmission fidelity and genome stability. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

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