BRDT (NM_001242805) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	BRD6; CT9; SPGF21
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC235071 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCTGCCAAGTCGACAAACAGCTATTATTGTTAACCCTCCTCCACCAGAATATATAAATACTAAGA AAAATGGGCGATTGACAAATCAACTTCAGTATCTACAAAAAGTTGTCCTAAAGGATTTATGGAAGCATAG TTTTTCATGGCCCTTTCAACGTCCTGTGGATGCTGTGAAACTACAGTTGCCTGATTATTATACCATTATA AAAAACCCAATGGATTTAAATACAATTAAGAAGCGCTTGGAGAATAAATATTATGCGAAGGCTTCAGAAT GTATAGAAGACTTCAATACAATGTTCTCAAATTGTTATTTATATAACAAGCCTGGAGATGACATTGTTCT TATGGCACAAGCTCTAGAGAAGCTGTTTATGCAGAAATTATCTCAGATGCCACAAGAAGAGCAAGTTGTG GGTGTTAAGGAAAGAATCAAGAAAGGCACTCAACAGAATATAGCTGTTTCTTCTGCTAAAGAAAAATCAT CACCCAGCGCAACAGAAAAAGTATTTAAGCAGCAAGAAATTCCTTCTGTATTTCCTAAGACATCTATTTC TCCCTTGAACGTGGTACAGGGAGCTTCAGTCAACTCCAGTTCACAAACTGCGGCCCAAGTTACAAAAGGT GTGAAGAGGAAAGCAGATACAACAACTCCTGCAACTTCAGCAGTTAAAGCAAGTAGTGAATTTTCTCCAA CATTCACAGAAAAATCAGTGGCACTGCCACCTATAAAAGAAAATATGCCAAAGAATGTTTTGCCAGATTC TCAGCAACAATATAATGTTGTGAAGACTGTTAAAGTAACTGAACAATTAAGGCACTGTAGTGAGATTCTT AAAGAAATGCTTGCAAAGAAACATTTTTCATATGCATGGCCCTTTTATAATCCTGTTGACGTTAATGCTT TGGGACTCCATAACTACTATGACGTTGTCAAAAATCCGATGGATCTTGGAACTATTAAGGAGAAAATGGA TAACCAAGAATATAAGGATGCATACAAATTTGCGGCAGATGTTAGATTAATGTTCATGAATTGCTACAAG TACAATCCTCCAGATCACGAAGTTGTGACAATGGCAAGAATGCTTCAGGATGTTTTCGAAACGCATTTTT CAAAGATCCCGATTGAACCTGTTGAGAGTATGCCTTTATGTTACATCAAAACAGATATCACAGAAACCAC TGGTAGAGAGAACACTAATGAAGCCTCCTCTGAAGGGAACTCTTCTGATGATTCTGAAGATGAGCGAGTT AAGCGTCTTGCAAAGCTTCAGGAGCAGCTTAAAGCTGTACATCAACAGCTCCAGGTTTTGTCCCAAGTAC CTTTCCGTAAGCTAAATAAAAAGAAAGAGAAGTCTAAAAAGGAAAAGAAAAAAGAAAAGGTTAATAACAG CAATGAAAATCCAAGAAAAATGTGTGAGCAAATGAGGCTAAAGGAAAAGTCCAAGAGAAATCAGCCAAAG AAAAGGAAACAACAGTTCATTGGTCTAAAATCTGAAGATGAAGATAATGCTAAACCTATGAACTATGATG AGAAAAGGCAGTTAAGTCTGAATATAAACAAACTCCCTGGAGATAAACTTGGGCGAGTAGTTCACATAAT ACAATCAAGAGAGCCTTCTCTGAGCAATTCCAATCCTGATGAGATAGAGATAGACTTTGAAACACTGAAA GCATCAACACTAAGAGAATTAGAAAAATATGTTTCGGCATGTCTAAGAAAGAGACCATTAAAACCTCCTG CTAAGAAAATAATGATGTCCAAAGAAGAACTTCACTCACAGAAAAAACAGGAATTGGAAAAGCGGTTACT GGATGTTAATAATCAGTTAAATTCTAGAAAACGTCAAACAAAATCTGATAAAACGCAACCATCCAAAGCT GTTGAAAATGTTTCCCGACTGAGTGAGAGCAGCAGCAGCAGCAGCAGCTCATCAGAGTCTGAAAGTAGCA GCAGTGACTTAAGCTCTTCAGACAGCAGTGATTCTGAATCAGAAATGTTCCCTAAGTTTACAGAAGTAAA ACCAAATGATTCTCCTTCTAAAGAGAATGTAAAGAAAATGAAGAATGAATGCATACTGCCTGAAGGAAGA ACAGGCGTCACACAGATAGGATATTGTGTGCAAGACACAACCTCTGCCAATACTACCCTTGTTCATCAGA CCACACCTTCACATGTAATGCCACCAAATCACCACCAATTAGCATTTAATTATCAAGAATTAGAACATTT ACAGACTGTGAAAAACATTTCACCTTTACAAATTCTGCCTCCCTCAGGTGATTCTGAACAGCTCTCAAAT GGCATAACTGTGATGCATCCATCTGGTGATAGTGACACAACGATGTTAGAATCTGAATGTCAAGCTCCTG TACAGAAGGATATAAAGATTAAGAATGCAGATTCATGGAAAAGTTTAGGCAAACCAGTGAAACCATCAGG TGTAATGAAATCCTCAGATGAGCTCTTCAACCAATTTAGAAAAGCAGCCATAGAAAAGGAAGTAAAAGCT CGGACACAGGAACTCATACGGAAGCATTTGGAACAAAATACAAAGGAACTAAAAGCATCTCAAGAAAATC AGAGGGATCTTGGGAATGGATTGACTGTAGAATCTTTTTCAAATAAAATACAAAACAAGTGCTCTGGAGA AGAGCAGAAAGAACATCAGCAGTCATCAGAAGCTCAAGATAAATCCAAACTCTGGCTTCTCAAAGACCGT GATTTAGCAAGGCAGAAAGAACAAGAGAGGAGGAGGAGAGAAGCAATGGTGGGTACCATTGATATGACCC TTCAAAGTGACATTATGACAATGTTTGAAAACAACTTTGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC235071 protein sequence Red=Cloning site Green=Tags(s) MSLPSRQTAIIVNPPPPEYINTKKNGRLTNQLQYLQKVVLKDLWKHSFSWPFQRPVDAVKLQLPDYYTII KNPMDLNTIKKRLENKYYAKASECIEDFNTMFSNCYLYNKPGDDIVLMAQALEKLFMQKLSQMPQEEQVV GVKERIKKGTQQNIAVSSAKEKSSPSATEKVFKQQEIPSVFPKTSISPLNVVQGASVNSSSQTAAQVTKG VKRKADTTTPATSAVKASSEFSPTFTEKSVALPPIKENMPKNVLPDSQQQYNVVKTVKVTEQLRHCSEIL KEMLAKKHFSYAWPFYNPVDVNALGLHNYYDVVKNPMDLGTIKEKMDNQEYKDAYKFAADVRLMFMNCYK YNPPDHEVVTMARMLQDVFETHFSKIPIEPVESMPLCYIKTDITETTGRENTNEASSEGNSSDDSEDERV KRLAKLQEQLKAVHQQLQVLSQVPFRKLNKKKEKSKKEKKKEKVNNSNENPRKMCEQMRLKEKSKRNQPK KRKQQFIGLKSEDEDNAKPMNYDEKRQLSLNINKLPGDKLGRVVHIIQSREPSLSNSNPDEIEIDFETLK ASTLRELEKYVSACLRKRPLKPPAKKIMMSKEELHSQKKQELEKRLLDVNNQLNSRKRQTKSDKTQPSKA VENVSRLSESSSSSSSSSESESSSSDLSSSDSSDSESEMFPKFTEVKPNDSPSKENVKKMKNECILPEGR TGVTQIGYCVQDTTSANTTLVHQTTPSHVMPPNHHQLAFNYQELEHLQTVKNISPLQILPPSGDSEQLSN GITVMHPSGDSDTTMLESECQAPVQKDIKIKNADSWKSLGKPVKPSGVMKSSDELFNQFRKAAIEKEVKA RTQELIRKHLEQNTKELKASQENQRDLGNGLTVESFSNKIQNKCSGEEQKEHQQSSEAQDKSKLWLLKDR DLARQKEQERRRREAMVGTIDMTLQSDIMTMFENNFD TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001242805
ORF Size	2841 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001242805.1, NP_001229734.1
RefSeq Size	3430 bp
RefSeq ORF	2844 bp
Locus ID	676
UniProt ID	Q58F21
Protein Families	Protein Kinase, Transcription Factors
MW	108 kDa
Gene Summary	BRDT is similar to the RING3 protein family. It possesses 2 bromodomain motifs and a PEST sequence (a cluster of proline, glutamic acid, serine, and threonine residues), characteristic of proteins that undergo rapid intracellular degradation. The bromodomain is found in proteins that regulate transcription. Several transcript variants encoding multiple isoforms have been found for this gene. [provided by RefSeq, Jun 2011]

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