BORA (NM_001286746) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	C13orf34
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239161 representing NM_001286746 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAGGTTTCTTTTCCCAGCTCCTGACTTGCCTGCCCGCTCGCCCCTGGTGAATGGGAGCAGTCAGG GAGTAGGTGTGGAAGAGAGGGGCACCAGAAAGAGTGGCCACGTAGGGTTGGGGGCGACGCCTCCTTCGCA CTCCATCCAGGGAAGCCGGCTGCATCTCAACCTTCCAGAAGTAGACTCTTTTCCACCCCACCCCGCCAAG TTTTCTCTCTGTGCTATGGGAGATGTCAAGGAATCAAAGATGCAAATAACACCAGAAACTCCAGGAAGGA TCCCTGTTTTAAATCCTTTTGAAAGTCCTAGTGATTATTCTAATCTCCATGAACAAACTCTCGCCAGTCC TTCTGTTTTTAAATCAACAAAATTACCAACTCCAGGGAAATTTAGATGGTCTATTGATCAACTAGCTGTA ATAAATCCTGTAGAAATAGACCCAGAAGATATTCATCGTCAAGCTTTATACTTAAGTCATTCTCGAATAG ATAAAGATGTGGAAGACAAAAGACAAAAAGCCATTGAAGAGTTTTTCACTAAAGATGTCATCGTACCCTC TCCTTGGACTGATCATGAAGGGAAACAGCTTTCACAATGTCATTCCAGTAAATGCACTAACATAAATAGT GACTCTCCAGTTGGAAAAAAGCTGACCATTCATTCTGAGAAAAGCGATGCTGCTTGTCAGACATTGCTGT CTCTTCCTGTGGATTTTAATTTAGAAAATATATTAGGTGACTATTTTAGAGCTGATGAATTTGCAGATCA ATCTCCTGGAAACCTCAGTTCTTCATCCCTCAGAAGAAAGCTGTTTTTAGATGGGAACGGAAGCATCTCC GACTCCTTACCTTCGGCTTCTCCCGGAAGTCCTCACAGTGGTGTTCAAACATCACTAGAGATGTTTTATT CAATAGATTTGTCTCCTGTAAAGTGTAGGAGCCCCTTGCAGACACCAAGTTCGGGGCAGTTTTCTTCTAG CCCTATTCAGGCTAGTGCAAAAAAATACAGCTTGGGAAGCATAACTAGTCCTTCGCCTATTTCTTCACCC ACTTTCTCACCAATTGAATTTCAGATAGGAGAGACTCCACTCTCAGAACAAAGGAAGTTTACTGTTCATT CTCCTGATGCTTCATCTGGAACAAATTCTAATGGGATAACTAATCCGTGTATCAGAAGTCCTTATATAGA TGGCTGCTCGCCAATTAAAAATTGGTCTCCTATGAGACTTCAGATGTATAGTGGTGGTACTCAGTATCGG ACCTCAGTGATTCAGATACCTTTTACTCTTGAGACTCAAGGTGAAGATGAGGAAGATAAAGAGAATATTC CTTCCACAGATGTCTCATCACCCGCCATGGATGCTGCTGGAATACACCTACGGCAGTTTAGTAATGAGGC TTCTACCCATGGTACACATTTGGTTGTGACTGCCATGTCTGTTACACAAAATCAGTCCAGTGCTTCTGAG AAAGAATTAGCACTGTTGCAGGATGTTGAAAGGGAGAAAGACAATAACACTGTGGATATGGTTGATCCTA TAGAGATAGCAGATGAGACCACTTGGATTAAGGAGCCGGTTGATAATGGCAGTTTACCCATGACTGATTT TGTAAGTGGCATTGCCTTCAGTATTGAAAACTCTCATATGTGCATGTCACCTCTTGCTGAAAGCAGTGTC ATTCCTTGTGAAAGCAGTAACATTCAGATGGATAGTGGCTATAATACGCAGAATTGTGGAAGCAATATTA TGGATACAGTTGGGGCAGAAAGTTACTGCAAAGAAAGTGATGCACAAACATGTGAAGTTGAGAGTAAATC TCAAGCATTTAATATGAAGCAAGACCACACAACACAGAGGTGTTGGATGAAAACAGCAAGCCCTTTTCAA TGCAGCAGTCCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239161 representing NM_001286746 Red=Cloning site Green=Tags(s) MERFLFPAPDLPARSPLVNGSSQGVGVEERGTRKSGHVGLGATPPSHSIQGSRLHLNLPEVDSFPPHPAK FSLCAMGDVKESKMQITPETPGRIPVLNPFESPSDYSNLHEQTLASPSVFKSTKLPTPGKFRWSIDQLAV INPVEIDPEDIHRQALYLSHSRIDKDVEDKRQKAIEEFFTKDVIVPSPWTDHEGKQLSQCHSSKCTNINS DSPVGKKLTIHSEKSDAACQTLLSLPVDFNLENILGDYFRADEFADQSPGNLSSSSLRRKLFLDGNGSIS DSLPSASPGSPHSGVQTSLEMFYSIDLSPVKCRSPLQTPSSGQFSSSPIQASAKKYSLGSITSPSPISSP TFSPIEFQIGETPLSEQRKFTVHSPDASSGTNSNGITNPCIRSPYIDGCSPIKNWSPMRLQMYSGGTQYR TSVIQIPFTLETQGEDEEDKENIPSTDVSSPAMDAAGIHLRQFSNEASTHGTHLVVTAMSVTQNQSSASE KELALLQDVEREKDNNTVDMVDPIEIADETTWIKEPVDNGSLPMTDFVSGIAFSIENSHMCMSPLAESSV IPCESSNIQMDSGYNTQNCGSNIMDTVGAESYCKESDAQTCEVESKSQAFNMKQDHTTQRCWMKTASPFQ CSSP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286746
ORF Size	1902 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286746.1, NP_001273675.1
RefSeq Size	3052 bp
RefSeq ORF	1680 bp
Locus ID	79866
UniProt ID	Q6PGQ7
Protein Families	Druggable Genome
MW	69.6 kDa
Gene Summary	BORA is an activator of the protein kinase Aurora A (AURKA; MIM 603072), which is required for centrosome maturation, spindle assembly, and asymmetric protein localization during mitosis (Hutterer et al., 2006 [PubMed 16890155]).[supplied by OMIM, Mar 2008]

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