Dynein intermediate chain 1 (DNAI1) (NM_001281428) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CILD1; DIC1; ICS1; PCD
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239358 representing NM_001281428 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTCCTGCTTCTGCGAAGGCTCCCCATAAACAGCCTCATAAGCAGAGCATCAGCATAGGCAGAGGAA CCAGGAAGAGAGATGAAGATTCAGGGACTGAAGTGGGAGAAGGCACAGATGAATGGGCCCAATCCAAAGC CACAGTTAGACCCCCTGACCAGCTGGAGTTGACCGATGCGGAGTTAAAGGAGGAGTTCACTCGGATTTTG ACAGCCAACAACCCACACGCACCCCAGAACATTGTCAGGTACAGCTTCAAAGAAGGCACATATAAGCCTA TTGGCTTTGTGAACCAACTGGCAGTTCACTACACCCAGGTTGGGAACCTGATCCCCAAAGACTCAGATGA AGGACGGCGGCAGCATTACCGCGATGAATTAGTGGCAGTATCCTACCAAGGTTCTCAGGAGTCTGTCAAG GTGATTTCAGAAACAGGAAACCTCGAAGAAGACGAAGAGCCCAAGGAGTTAGAAACTGAGCCTGGGAGTC AAACAGATGTGCCTGCAGCTGGGGCAGCTGAAAAAGTGACTGAAGAAGAATTGATGACTCCTAAGCAGCC CAAGGAGAGAAAGCTCACTAACCAGTTCAACTTCAGTGAGAGGGCCTCACAGACCTACAACAACCCTGTC CGGGATCGAGAATGCCAGACGGAGCCTCCTCCCAGGACAAACTTTTCAGCCACAGCCAATCAGTGGGAGA TCTATGATGCCTATGTAGAGGAACTTGAGAAGCAGGAAAAGACCAAAGAGAAGGAGAAGGCAAAGACCCC AGTGGCTAAAAAATCAGGGAAGATGGCCATGAGGAAGCTGACATCTATGGAGTCTCAGACTGATGATCTC ATCAAATTGTCCCAAGCTGCTAAGATCATGGAGCGGATGGTCAACCAGAATACATATGATGACATTGCTC AAGATTTTAAGTACTATGACGATGCTGCTGATGAATACCGGGACCAGGTGGGTACCCTGCTGCCGCTCTG GAAGTTCCAAAATGACAAAGCCAAGCGCCTGTCCGTCACTGCCCTCTGCTGGAATCCAAAGTACAGGGAT CTGTTTGCAGTGGGATATGGCTCTTATGACTTCATGAAGCAGAGCCGGGGCATGCTGCTGCTCTACAGCC TGAAGAACCCCAGCTTCCCTGAGTACATGTTCAGCAGCAACAGCGGCGTCATGTGTCTCGACATCCACGT GGACCACCCCTACCTGGTGGCAGTAGGCCACTATGACGGCAACGTGGCCATTTACAACCTCAAGAAGCCC CACTCCCAGCCCTCCTTCTGCAGCTCAGCCAAGTCTGGCAAGCACTCAGACCCTGTGTGGCAGGTCAAGT GGCAGAAGGATGACATGGACCAAAACCTTAACTTCTTCTCTGTGTCATCTGACGGCAGGATTGTGTCTTG GACTCTCGTGAAGAGAAAGCTGGTTCACATAGATGTCATCAAGCTGAAGGTGGAAGGCAGCACCACGGAA GTTCCTGAGGGGTTGCAGCTGCACCCAGTGGGTTGTGGCACTGCCTTTGACTTCCACAAAGAGATTGACT ACATGTTCCTAGTGGGCACAGAGGAGGGAAAAATCTACAAGTGCTCTAAATCCTACTCCAGCCAATTCCT CGACACCTATGACGCCCACAACATGTCAGTGGACACTGTGTCCTGGAACCCATACCACACCAAGGTCTTC ATGTCCTGCAGCTCCGACTGGACAGTGAAGATCTGGGACCACACCATCAAGACCCCGATGTTCATCTATG ACCTGAACTCAGCCGTGGGTGATGTGGCCTGGGCGCCATACTCTTCTACTGTGTTCGCAGCAGTCACCAC AGATGGGAAGGCCCACATATTTGACTTAGCCATCAACAAGTATGAGGCCATCTGCAACCAGCCTGTGGCG GCCAAAAAGAACAGGCTCACCCACGTGCAGTTCAATCTCATCCACCCCATCATCATTGTGGGCGATGACC GTGGGCACATCATCAGCCTCAAGCTCTCACCCAATTTGCGCAAGATGCCAAAGGAAAAGAAGGGGCAGGA GGTGCAGAAGGGTCCAGCTGTGGAGATTGCGAAACTGGACAAACTGCTGAACCTGGTGAGGGAAGTGAAA ATCAAGACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239358 representing NM_001281428 Red=Cloning site Green=Tags(s) MIPASAKAPHKQPHKQSISIGRGTRKRDEDSGTEVGEGTDEWAQSKATVRPPDQLELTDAELKEEFTRIL TANNPHAPQNIVRYSFKEGTYKPIGFVNQLAVHYTQVGNLIPKDSDEGRRQHYRDELVAVSYQGSQESVK VISETGNLEEDEEPKELETEPGSQTDVPAAGAAEKVTEEELMTPKQPKERKLTNQFNFSERASQTYNNPV RDRECQTEPPPRTNFSATANQWEIYDAYVEELEKQEKTKEKEKAKTPVAKKSGKMAMRKLTSMESQTDDL IKLSQAAKIMERMVNQNTYDDIAQDFKYYDDAADEYRDQVGTLLPLWKFQNDKAKRLSVTALCWNPKYRD LFAVGYGSYDFMKQSRGMLLLYSLKNPSFPEYMFSSNSGVMCLDIHVDHPYLVAVGHYDGNVAIYNLKKP HSQPSFCSSAKSGKHSDPVWQVKWQKDDMDQNLNFFSVSSDGRIVSWTLVKRKLVHIDVIKLKVEGSTTE VPEGLQLHPVGCGTAFDFHKEIDYMFLVGTEEGKIYKCSKSYSSQFLDTYDAHNMSVDTVSWNPYHTKVF MSCSSDWTVKIWDHTIKTPMFIYDLNSAVGDVAWAPYSSTVFAAVTTDGKAHIFDLAINKYEAICNQPVA AKKNRLTHVQFNLIHPIIIVGDDRGHIISLKLSPNLRKMPKEKKGQEVQKGPAVEIAKLDKLLNLVREVK IKT TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001281428
ORF Size	2109 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001281428.1, NP_001268357.1
RefSeq Size	2605 bp
RefSeq ORF	2112 bp
Locus ID	27019
UniProt ID	Q9UI46
Protein Families	Druggable Genome
Protein Pathways	Huntington's disease
MW	80.2 kDa
Gene Summary	This gene encodes a member of the dynein intermediate chain family. The encoded protein is part of the dynein complex in respiratory cilia. The inner- and outer-arm dyneins, which bridge between the doublet microtubules in axonemes, are the force-generating proteins responsible for the sliding movement in axonemes. The intermediate and light chains, thought to form the base of the dynein arm, help mediate attachment and may also participate in regulating dynein activity. Mutations in this gene result in abnormal ciliary ultrastructure and function associated with primary ciliary dyskinesia and Kartagener syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.