Apc4 (ANAPC4) (NM_001286756) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	APC4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239609 representing NM_001286756 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGCGTTTTCCGACCTGTTTCCCATCCTTCCGGGTGGTGGGAGAGAAGCAGCTCCCGCAGGAGATTA TTTTCCTGGTCTGGTCGCCCAAGCGGGATCTCATTGCTTTGGCCAACACAGCTGGCGAGGTTTTACTTCA TCGACTGGCAAGTTTTCATCGAGTTTGGAGTTTTCCACCAAATGAAAATACAGGAAAGGAGGTGACGTGT CTGGCATGGAGACCAGATGGCAAACTTTTGGCCTTTGCTCTTGCTGATACCAAGAAAATTGTTTTGTGTG ATGTAGAAAAACCTGAGAGCTTACACTCTTTTTCTGTGGAGGCTCCAGTTTCCTGTATGCATTGGATGGA AGTGACAGTAGAAAGCAGTGTTCTCACATCATTTTATAATGCTGAGGATGAATCAAATCTTCTCTTACCT AAACTACCTACACTGCCAAAAAACTATAGCAACACCTCAAAAATATTTAGTGAAGAAAATTCTGATGAAA TTATTAAGCTCTTGGGAGACGTCAGGCTTAATATTCTCGTCCTTGGAGGAAGCTCTGGATTTATTGAGCT TTATGCTTATGGAATGTTTAAAATTGCTCGAGTCACAGGGATTGCTGGTACTTGTCTTGCATTATGTTTA TCAAGTGATTTGAAATCATTATCAGTGGTCACAGAAGTCTCTACCAATGGTGCTTCAGAAGTTTCATACT TTCAGCTTGAAACTAATCTGTTGTACTCTTTCTTACCTGAAGTAACTCGGATGGCCAGAAAGTTTACTCA TATTTCAGCTCTGTTACAGTATATAAATTTGTCACTAACATGTATGTGTGAAGCATGGGAAGAAATACTA ATGCAGATGGATTCTCGTCTCACCAAGTTTGTGCAGGAAAAGAACACAACCACATCAGTGCAAGATGAGT TCATGCACTTGCTATTATGGGGGAAAGCAAGTGCTGAACTTCAGACTCTCTTGATGAATCAGTTAACAGT AAAGGGCTTGAAAAAGCTTGGCCAGTCTATAGAGTCATCATACTCCAGTATACAAAAATTGGTCATAAGT CATTTACAGAGTGGCTCGGAGTCTTTATTATACCATTTGAGTGAATTGAAAGGAATGGCTTCATGGAAGC AAAAATATGAACCTCTTGGACTAGATGCTGCAGGAATCGAAGAAGCTATAACTGCTGTGGGTTCTTTTAT ACTCAAGGCAAATGAACTTCTTCAAGTTATAGATAGTAGTATGAAAAACTTCAAAGCATTTTTTCGGTGG CTTTATGTGGCAATGTTGAGAATGACAGAAGACCATGTGCTTCCCGAGCTGAATAAGGTAATGACTCAGA AAGATATCACATTTGTTGCTGAATTTCTTACTGAACATTTCAATGAGGCTCCAGACCTTTATAATCGAAA AGGAAAATACTTTAACGTTGAAAGAGTTGGTCAGTACTTGAAAGATGAAGATGATGATCTTGTGTCACCC CCTAACACAGAAGGAAACCAGTGGTATGACTTTCTTCAAAATAGCAGCCACCTTAAAGAAAGTCCTTTGC TGTTTCCTTATTATCCTCGAAAATCATTGCATTTTGTGAAAAGGCGGATGGAGAATATTATTGATCAGTG TTTGCAAAAGCCAGCAGATGTAATTGGAAAATCGATGAATCAAGCAATCTGTATTCCATTGTATAGAGAT ACCAGAAGTGAGGATTCTACACGTAGATTGTTCAAATTTCCTTTTCTGTGGAATAATAAAACTTCAAATC TACATTATCTTCTTTTTACTATTCTAGAAGATTCACTTTATAAAATGTGCATCTTAAGGAGACATACTGA TATTTCTCAATCTGTGAGTAATGGACTAATTGCTATTAAATTTGGGAGCTTTACATATGCCACAACAGAA AAAGTCAGAAGAAGCATCTACAGTTGTTTAGATGCACAGTTTTATGATGATGAAACTGTAACAGTAGTTC TTAAAGACACTGTAGGACGTGAAGGAAGAGATAGACTCTTGGTCCAGCTGCCTTTGTCTTTAGTATATAA CAGTGAAGATTCTGCAGAATATCAGTTCACTGGGACTTATTCTACAAGGCTAGATGAACAGTGTAGTGCT ATTCCCACCCGTACCATGCATTTTGAGAAGCACTGGAGATTACTGGAAAGTATGAAAGCACAGTATGTTG CTGGGAATGGTTTTCGAAAAGTGTCCTGTGTGTTAAGCTCAAATCTTCGTCATGTGAGAGTATTTGAAAT GGACATAGATGATGAATGGGAGCTCGATGAGTCTTCAGATGAAGAGGAGGAGGCCAGTAATAAGCCTGTA AAAATAAAGGAAGAAGTGTTGTCGGAGTCAGAGGCAGAGAACCAACAAGCTGGTGCTGCCGCTTTAGCTC CAGAGATAGTCATTAAAGTGGAAAAACTTGACCCTGAGCTAGACTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239609 representing NM_001286756 Red=Cloning site Green=Tags(s) MLRFPTCFPSFRVVGEKQLPQEIIFLVWSPKRDLIALANTAGEVLLHRLASFHRVWSFPPNENTGKEVTC LAWRPDGKLLAFALADTKKIVLCDVEKPESLHSFSVEAPVSCMHWMEVTVESSVLTSFYNAEDESNLLLP KLPTLPKNYSNTSKIFSEENSDEIIKLLGDVRLNILVLGGSSGFIELYAYGMFKIARVTGIAGTCLALCL SSDLKSLSVVTEVSTNGASEVSYFQLETNLLYSFLPEVTRMARKFTHISALLQYINLSLTCMCEAWEEIL MQMDSRLTKFVQEKNTTTSVQDEFMHLLLWGKASAELQTLLMNQLTVKGLKKLGQSIESSYSSIQKLVIS HLQSGSESLLYHLSELKGMASWKQKYEPLGLDAAGIEEAITAVGSFILKANELLQVIDSSMKNFKAFFRW LYVAMLRMTEDHVLPELNKVMTQKDITFVAEFLTEHFNEAPDLYNRKGKYFNVERVGQYLKDEDDDLVSP PNTEGNQWYDFLQNSSHLKESPLLFPYYPRKSLHFVKRRMENIIDQCLQKPADVIGKSMNQAICIPLYRD TRSEDSTRRLFKFPFLWNNKTSNLHYLLFTILEDSLYKMCILRRHTDISQSVSNGLIAIKFGSFTYATTE KVRRSIYSCLDAQFYDDETVTVVLKDTVGREGRDRLLVQLPLSLVYNSEDSAEYQFTGTYSTRLDEQCSA IPTRTMHFEKHWRLLESMKAQYVAGNGFRKVSCVLSSNLRHVRVFEMDIDDEWELDESSDEEEEASNKPV KIKEEVLSESEAENQQAGAAALAPEIVIKVEKLDPELDS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286756
ORF Size	2427 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286756.2
RefSeq Size	2704 bp
RefSeq ORF	2430 bp
Locus ID	29945
UniProt ID	Q9UJX5
Protein Families	Druggable Genome
Protein Pathways	Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis
MW	92.7 kDa
Gene Summary	A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The exact function of this gene product is not known. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]

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