Epac2 (RAPGEF4) (NM_001282900) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2; Nbla00496
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239661 representing NM_001282900 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGGACTTCTGGCTCCTCCTTATGGTGTTATGGAAACGGGCTCTAACAATGACAGGATTCCTGACA AGGAGAACGTCCCTTCAGAGAAGATCCTCAGAGCTGGAAAAATTTTACGAAATGCCATTCTCTCTCGAGC ACCTCACATGATAAGAGATAGAAAATACCACCTAAAGACATACAGACAATGCTGTGTGGGAACTGAACTG GTGGACTGGATGATGCAGCAGACACCATGTGTTCACTCCCGGACTCAAGCTGTTGGCATGTGGCAAGTCC TGTTAGAAGATGGTGTTCTCAACCACGTGGACCAGGAGCACCATTTCCAAGACAAATATTTATTCTATCG ATTTCTGGATGATGAGCACGAGGATGCCCCTTTGCCTACTGAGGAGGAGAAGAAGGAGTGTGATGAGGAG CTCCAGGACACCATGCTGCTGCTGTCACAGATGGGCCCCGACGCCCACATGAGGATGATCCTTCGCAAAC CACCTGGCCAGAGGACTGTGGATGACCTAGAGATTATCTATGAGGAGCTTCTTCATATTAAAGCCTTATC CCATCTTTCTACCACAGTGAAACGAGAGTTAGCAGGTGTTCTCATTTTTGAGTCTCACGCCAAAGGAGGG ACTGTGTTGTTTAACCAGGGGGAAGAAGGTACCTCCTGGTACATTATTCTAAAAGGATCAGTGAATGTAG TCATTTACGGCAAGGGTGTGGTCTGCACCCTGCATGAAGGAGATGACTTCGGCAAGTTAGCACTAGTGAA TGATGCCCCACGAGCTGCCTCTATCGTCTTACGAGAAGATAACTGCCATTTCTTAAGAGTAGACAAGGAG GATTTCAACCGGATCCTAAGGGACGTGGAGGCGAATACAGTCAGACTTAAAGAACATGACCAAGATGTCT TGGTGCTGGAGAAGGTCCCAGCAGGGAACAGAGCTTCTAATCAAGGAAACTCACAGCCTCAGCAAAAGTA TACTGTGATGTCAGGAACACCTGAAAAAATTTTAGAGCATTTTCTAGAAACAATACGCCTTGAGGCAACT TTAAATGAAGCAACAGATTCTGTTTTAAATGACTTTATTATGATGCACTGTGTTTTTATGCCAAATACCC AGCTTTGCCCGGCACTGGTGGCCCACTACCACGCACAGCCTTCACAAGGTACAGAACAGGAGAAAATGGA TTATGCCCTCAACAATAAGAGGCGAGTCATCCGCCTGGTTCTACAGTGGGCTGCCATGTATGGAGACCTC CTGCAAGAGGATGACGTGTCTATGGCCTTCCTGGAGGAGTTTTATGTATCTGTATCAGATGATGCCCGGA TGATTGCTGCCCTCAAGGAGCAACTGCCAGAGTTGGAGAAGATTGTCAAGCAAATCTCAGAAGATGCAAA GGCACCACAAAAGAAGCACAAGGTTCTTTTGCAACAGTTCAATACGGGCGATGAGAGAGCCCAGAAGCGC CAGCCTATCCGCGGCTCTGATGAAGTTCTGTTTAAGGTCTATTGCATGGACCACACCTACACAACCATTC GGGTGCCAGTGGCCACTTCGGTGAAGGAAGTCATCAGTGCAGTTGCCGACAAGCTGGGCTCCGGGGAGGG CCTGATCATAGTCAAGATGAGTTCCGGAGGAGAAAAGGTGGTGCTCAAACCTAATGATGTTTCAGTATTT ACGACGCTCACCATTAATGGACGCCTGTTTGCTTGCCCGCGAGAGCAATTCGATTCACTGACTCCCTTAC CAGAACAGGAAGGCCCAACTGTTGGAACAGTGGGAACTTTTGAACTGATGAGCTCCAAAGATTTAGCATA CCAGATGACAATTTATGATTGGGAACTCTTCAACTGCGTGCATGAGCTGGAGCTAATCTATCACACATTT GGAAGGCATAATTTTAAAAAGACCACAGCAAACTTGGATTTGTTCCTGAGGAGATTTAATGAAATTCAGT TTTGGGTCGTCACTGAGATCTGCCTTTGTTCTCAGCTCAGCAAGCGTGTTCAGCTATTAAAAAAATTTAT TAAGATAGCAGCCCACTGTAAGGAGTATAAAAATCTGAATTCCTTTTTTGCCATCGTCATGGGACTAAGT AACGTTGCTGTGAGCCGCTTGGCACTAACGTGGGAGAAACTGCCAAGCAAGTTCAAGAAGTTCTATGCGG AGTTTGAAAGTTTAATGGACCCTTCAAGGAACCACAGGGCCTACAGGCTGACAGTAGCTAAGCTGGAACC TCCTCTCATCCCCTTCATGCCTTTGCTCATTAAAGATATGACATTTACTCATGAGGGGAACAAGACGTTC ATTGACAATCTAGTAAACTTTGAAAAAATGCGCATGATTGCAAATACGGCCAGAACAGTGAGATACTACA GGAGCCAACCCTTCAATCCTGATGCAGCTCAAGCTAATAAGAACCATCAGGATGTCCGGAGTTATGTACG GCAATTAAATGTGATTGACAACCAGAGAACTTTATCACAGATGTCACACAGATTAGAGCCTCGTCGACCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239661 representing NM_001282900 Red=Cloning site Green=Tags(s) MAGLLAPPYGVMETGSNNDRIPDKENVPSEKILRAGKILRNAILSRAPHMIRDRKYHLKTYRQCCVGTEL VDWMMQQTPCVHSRTQAVGMWQVLLEDGVLNHVDQEHHFQDKYLFYRFLDDEHEDAPLPTEEEKKECDEE LQDTMLLLSQMGPDAHMRMILRKPPGQRTVDDLEIIYEELLHIKALSHLSTTVKRELAGVLIFESHAKGG TVLFNQGEEGTSWYIILKGSVNVVIYGKGVVCTLHEGDDFGKLALVNDAPRAASIVLREDNCHFLRVDKE DFNRILRDVEANTVRLKEHDQDVLVLEKVPAGNRASNQGNSQPQQKYTVMSGTPEKILEHFLETIRLEAT LNEATDSVLNDFIMMHCVFMPNTQLCPALVAHYHAQPSQGTEQEKMDYALNNKRRVIRLVLQWAAMYGDL LQEDDVSMAFLEEFYVSVSDDARMIAALKEQLPELEKIVKQISEDAKAPQKKHKVLLQQFNTGDERAQKR QPIRGSDEVLFKVYCMDHTYTTIRVPVATSVKEVISAVADKLGSGEGLIIVKMSSGGEKVVLKPNDVSVF TTLTINGRLFACPREQFDSLTPLPEQEGPTVGTVGTFELMSSKDLAYQMTIYDWELFNCVHELELIYHTF GRHNFKKTTANLDLFLRRFNEIQFWVVTEICLCSQLSKRVQLLKKFIKIAAHCKEYKNLNSFFAIVMGLS NVAVSRLALTWEKLPSKFKKFYAEFESLMDPSRNHRAYRLTVAKLEPPLIPFMPLLIKDMTFTHEGNKTF IDNLVNFEKMRMIANTARTVRYYRSQPFNPDAAQANKNHQDVRSYVRQLNVIDNQRTLSQMSHRLEPRRP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282900
ORF Size	2520 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282900.2
RefSeq Size	3937 bp
RefSeq ORF	2523 bp
Locus ID	11069
UniProt ID	Q8WZA2
Protein Pathways	Leukocyte transendothelial migration
MW	96.7 kDa
Gene Summary	Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2 (By similarity).[UniProtKB/Swiss-Prot Function]

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