GRID2 (NM_001286838) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	GluD2; SCAR18
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239771 representing NM_001286838 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGTTTTCCCCTTTCTCTTGGTTTTGTCCGTCTGGTGGTCTCGAACCTGGGACTCGGCGAATGCGG ATTCGATCATTCACATCGGAGCAATTTTTGATGAATCTGCCAAAAAGGATGATGAGGTATTTCGCACTGC GGTTGGTGACCTTAACCAGAATGAGGAGATCTTACAGACTGAGAAAATCACATTTTCAGTGACGTTTGTT GATGGCAACAACCCTTTCCAAGCAGTTCAAGAAGATATCCGTGGAATACAGGAGTTCTTGGACAAAGTCT CTCAGCAGGGAATGGATGTTGCACTTCAGAAGGTAGAAAACAACATCAATAAAATGATTACCACTCTCTT TGACACCATGAGAATAGAAGAACTGAATCGCTATCGAGACACTCTTAGGCGAGCGATCCTTGTTATGAAT CCTGCTACAGCCAAATCCTTCATTACTGAGGTTGTGGAGACTAATTTGGTTGCTTTTGACTGTCACTGGA TCATTATAAATGAGGAAATAAACGATGTGGACGTACAGGAACTTGTAAGAAGGTCAATTGGAAGGTTAAC GATTATTCGGCAGACATTTCCAGTTCCCCAGAACATAAGTCAGCGGTGTTTCCGTGGCAACCATCGAATA TCTTCAACATTGTGTGATCCAAAGGATCCATTTGCTCAGAATATGGAGATTTCCAACCTTTACATATATG ACACGGTGCTTCTGCTTGCTAATGCTTTTCATAAGAAGCTGGAGGACCGAAAGTGGCACAGCATGGCAAG TCTGTCATGTATCAGAAAGAACTCAAAGCCCTGGCAGGGTGGGCGCTCCATGTTGGAGACCATCAAGAAG GGTGGAGTTAGTGGGTTGACTGGAGAGCTAGAATTTGGAGAAAATGGAGGCAATCCCAATGTCCACTTTG AAATCCTTGGAACCAACTATGGAGAAGAGCTTGGCAGAGGTGTTCGAAAACTTGGTTGCTGGAATCCTGT CACAGGTCTGAATGGGTCACTGACTGACAAGAAATTGGAGAATAACATGCGTGGAGTGGTTCTACGTGTA GTAACTGTTCTGGAAGAACCTTTTGTGATGGTCTCTGAAAATGTCTTGGGTAAGCCGAAGAAATACCAGG GCTTCTCCATTGATGTTTTGGATGCCTTATCTAACTACCTGGGTTTTAACTACGAAATTTACGTAGCACC GGATCACAAATACGGAAGCCCACAAGAAGATGGGACATGGAATGGCTTGGTAGGAGAACTTGTCTTTAAG AGAGCCGACATAGGGATTTCTGCTTTAACCATCACTCCAGATCGTGAAAATGTGGTGGACTTTACGACAC GTTACATGGACTACTCAGTGGGGGTACTACTTCGAAGGGCTGAAAAGACAGTGGATATGTTTGCCTGTCT TGCACCATTTGATCTCTCTCTATGGGCTTGCATTGCTGGCACAGTCCTTCTGGTGGGTCTACTGGTCTAC CTCTTGAACTGGCTTAATCCCCCACGATTACAAATGGGATCAATGACGTCTACTACTCTCTACAACTCCA TGTGGTTTGTGTATGGATCTTTTGTACAACAAGGCGGGGAAGTCCCGTACACGACTCTGGCTACCCGAAT GATGATGGGGGCTTGGTGGCTATTTGCTTTGATTGTTATCTCATCTTACACGGCAAACCTCGCTGCTTTC CTCACTATTACACGCATTGAAAGTTCCATCCAGTCTCTCCAGGACCTTTCCAAGCAAACAGAAATCCCTT ATGGCACAGTCCTAGACTCTGCGGTATATGAGCATGTCCGCATGAAAGGACTGAATCCTTTTGAGAGGGA CAGCATGTATTCCCAAATGTGGCGGATGATCAACCGAAGCAATGGATCGGAGAACAATGTTCTGGAGTCC CAGGCAGGCATTCAAAAGGTAAAATATGGAAATTATGCTTTCGTATGGGATGCAGCTGTATTGGAATATG TGGCTATCAATGACCCAGATTGTTCCTTTTACACCATTGGAAATACTGTTGCTGATCGGGGATATGGAAT TGCATTACAACATGGCAGTCCTTACCGAGATGTTTTTTCACAAAGGATCCTGGAGCTTCAGCAGAATGGT GACATGGACATCCTGAAGCACAAATGGTGGCCTAAGAATGGCCAGTGTGACCTGTACTCGTCAGTGGACA CAAAGCAGAAAGGAGGCGCCCTGGACATAAAGAGCTTTGCAGGGGTCTTTTGTATCCTGGCTGCTGGAAT TGTCCTCTCCTGCTTCATAGCCATGCTGGAGACGTGGTGGAACAAGAGGAAAGGCTCCCGGGTTCCATCA AAAGAGGATGACAAGGAAATTGACCTGGAGCACCTCCATAGACGTGTAAATAGCTTGTGCACAGATGACG ACAGCCCCCATAAACAGTTTTCCACCTCGTCAATTGATTTGACCCCTCTGGACATTGACACTTTGCCAAC ACGACAAGCACTGGAGCAAATCAGTGATTTCAGGAACACTCATATTACCACAACAACCTTTATCCCAGAG CAGATCCAGACTCTTAGCCGCACACTGTCAGCTAAAGCTGCTTCTGGTTTCACTTTTGGCAACGTGCCTG AGCACCGAACTGGCCCTTTTAGGCACAGGGCACCTAATGGGGGCTTTTTCAGGAGTCCTATAAAAACAAT GTCATCTATTCCTTATCAACCAACTCCTACCCTGGGGCTCAATCTGGGTAATGATCCAGACCGAGGCACC TCCATA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239771 representing NM_001286838 Red=Cloning site Green=Tags(s) MEVFPFLLVLSVWWSRTWDSANADSIIHIGAIFDESAKKDDEVFRTAVGDLNQNEEILQTEKITFSVTFV DGNNPFQAVQEDIRGIQEFLDKVSQQGMDVALQKVENNINKMITTLFDTMRIEELNRYRDTLRRAILVMN PATAKSFITEVVETNLVAFDCHWIIINEEINDVDVQELVRRSIGRLTIIRQTFPVPQNISQRCFRGNHRI SSTLCDPKDPFAQNMEISNLYIYDTVLLLANAFHKKLEDRKWHSMASLSCIRKNSKPWQGGRSMLETIKK GGVSGLTGELEFGENGGNPNVHFEILGTNYGEELGRGVRKLGCWNPVTGLNGSLTDKKLENNMRGVVLRV VTVLEEPFVMVSENVLGKPKKYQGFSIDVLDALSNYLGFNYEIYVAPDHKYGSPQEDGTWNGLVGELVFK RADIGISALTITPDRENVVDFTTRYMDYSVGVLLRRAEKTVDMFACLAPFDLSLWACIAGTVLLVGLLVY LLNWLNPPRLQMGSMTSTTLYNSMWFVYGSFVQQGGEVPYTTLATRMMMGAWWLFALIVISSYTANLAAF LTITRIESSIQSLQDLSKQTEIPYGTVLDSAVYEHVRMKGLNPFERDSMYSQMWRMINRSNGSENNVLES QAGIQKVKYGNYAFVWDAAVLEYVAINDPDCSFYTIGNTVADRGYGIALQHGSPYRDVFSQRILELQQNG DMDILKHKWWPKNGQCDLYSSVDTKQKGGALDIKSFAGVFCILAAGIVLSCFIAMLETWWNKRKGSRVPS KEDDKEIDLEHLHRRVNSLCTDDDSPHKQFSTSSIDLTPLDIDTLPTRQALEQISDFRNTHITTTTFIPE QIQTLSRTLSAKAASGFTFGNVPEHRTGPFRHRAPNGGFFRSPIKTMSSIPYQPTPTLGLNLGNDPDRGT SI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286838
ORF Size	2736 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286838.1, NP_001273767.1
RefSeq Size	5069 bp
RefSeq ORF	2739 bp
Locus ID	2895
UniProt ID	O43424
Protein Families	Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane
Protein Pathways	Long-term depression, Neuroactive ligand-receptor interaction
MW	103.3 kDa
Gene Summary	The protein encoded by this gene is a member of the family of ionotropic glutamate receptors which are the predominant excitatory neurotransmitter receptors in the mammalian brain. The encoded protein is a multi-pass membrane protein that is expressed selectively in cerebellar Purkinje cells. A point mutation in the mouse ortholog, associated with the phenotype named 'lurcher', in the heterozygous state leads to ataxia resulting from selective, cell-autonomous apoptosis of cerebellar Purkinje cells during postnatal development. Mice homozygous for this mutation die shortly after birth from massive loss of mid- and hindbrain neurons during late embryogenesis. This protein also plays a role in synapse organization between parallel fibers and Purkinje cells. Alternate splicing results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause cerebellar ataxia in humans. [provided by RefSeq, Apr 2014]

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