GRIK5 (NM_001301030) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	EAA2; GluK5; GRIK2; KA2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239851 representing NM_001301030 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGCTGAGCTGCTGCTGCTGCTGATTGTTGCCTTCGCCAGCCCCAGCTGCCAGGTGCTCTCATCAC TGCGCATGGCTGCAATCCTGGATGATCAGACAGTGTGTGGCCGCGGTGAGCGTCTGGCCTTGGCCTTGGC CCGGGAGCAGATCAACGGGATCATCGAGGTCCCAGCCAAGGCCCGAGTGGAAGTAGACATCTTTGAGCTG CAGCGGGACAGCCAGTACGAGACCACGGACACCATGTGTCAGATCTTACCCAAAGGGGTTGTGTCTGTCC TTGGGCCCTCCTCTAGCCCAGCATCTGCCTCCACCGTGAGCCATATCTGTGGAGAGAAGGAGATCCCCCA CATCAAGGTGGGTCCCGAGGAGACACCCCGCCTTCAGTACCTTCGCTTCGCGTCTGTCAGCCTGTACCCC AGTAACGAGGACGTCAGCTTGGCGGTCTCCCGAATCCTCAAGTCCTTCAACTACCCCTCGGCCAGCCTCA TCTGCGCCAAGGCTGAGTGCCTGCTGCGATTGGAGGAACTGGTGCGTGGCTTCCTCATCTCCAAGGAGAC GCTGTCAGTGAGGATGTTGGACGACAGCCGGGACCCCACACCACTGCTCAAGGAGATCCGTGATGACAAG GTGTCCACCATCATCATCGACGCCAACGCCTCCATCTCCCACCTCATCCTCCGTAAGGCCTCGGAACTGG GAATGACCTCAGCGTTTTACAAGTACATCCTCACCACCATGGACTTCCCCATCCTGCATCTGGACGGTAT TGTGGAGGACTCCTCCAACATCCTGGGCTTCTCCATGTTCAACACGTCCCACCCCTTCTACCCTGAGTTT GTCCGCAGCCTCAACATGTCCTGGAGGGAGAACTGTGAAGCCAGCACCTACCTGGGCCCTGCGCTGTCAG CCGCCCTGATGTTTGACGCCGTGCACGTGGTGGTGAGCGCTGTCCGAGAGCTGAACCGCAGCCAGGAGAT CGGTGTGAAGCCTCTGGCCTGTACATCGGCCAACATTTGGCCCCACGGGACCAGCCTCATGAACTACCTG CGCATGGTAGAGTATGATGGGCTGACCGGGCGGGTCGAGTTCAACAGCAAAGGGCAGAGAACCAACTACA CCCTGCGCATCCTAGAAAAGTCCCGGCAGGGCCACCGTGAGATTGGGGTGTGGTACTCTAACCGCACCCT GGCCATGAATGCCACCACCCTGGACATCAACCTGTCGCAGACACTGGCCAACAAGACCCTGGTGGTCACA ACCATCCTGGAGAACCCATACGTCATGCGCCGGCCCAACTTCCAGGCCCTGTCGGGGAACGAACGCTTCG AGGGCTTCTGCGTGGACATGCTGCGGGAGCTGGCCGAGCTGCTGCGCTTCCGCTACCGCCTGCGGTTGGT GGAGGATGGGCTGTACGGGGCGCCCGAGCCCAACGGCTCCTGGACGGGCATGGTTGGCGAGCTCATCAAC CGGAAGGCAGACCTGGCTGTGGCCGCCTTCACCATCACAGCTGAGCGGGAGAAGGTCATCGACTTTTCCA AGCCCTTTATGACCCTGGGGATCAGCATCCTCTACCGAGTGCACATGGGCCGCAAGCCTGGCTACTTCTC CTTCCTGGACCCCTTCTCCCCTGCTGTGTGGCTCTTCATGCTTCTTGCCTACCTGGCTGTCAGCTGCGTC CTGTTTCTGGCTGCCAGGCTGAGCCCCTATGAGTGGTATAACCCACACCCATGCCTGCGGGCACGCCCCC ACATCCTGGAGAACCAGTACACGCTGGGCAACAGCCTGTGGTTTCCCGTGGGGGGCTTCATGCAGCAGGG CTCGGAGATCATGCCCCGGGCGCTGTCCACGCGCTGTGTCAGCGGAGTCTGGTGGGCCTTCACCTTGATC ATCATCTCCTCCTACACGGCCAACCTGGCCGCCTTCCTCACCGTGCAGCGCATGGAGGTGCCTGTGGAGT CGGCCGATGACCTGGCAGATCAGACCAACATCGAGTATGGCACCATCCACGCCGGCTCCACCATGACCTT CTTCCAGAATTCACGGTACCAAACGTACCAGCGCATGTGGAACTACATGCAGTCGAAGCAGCCCAGCGTG TTCGTCAAGAGCACAGAAGAGGGCATTGCCCGCGTCCTCAACTCCCGCTACGCCTTCCTGCTCGAGTCCA CCATGAACGAATACCACCGGCGCCTCAACTGCAACCTCACCCAGATCGGGGGACTCCTCGACACCAAGGG CTACGGCATTGGCATGCCGCTGGGCTCCCCGTTCCGGGATGAGATCACACTGGCCATCCTGCAGCTTCAG GAGAACAACCGGCTGGAGATCCTGAAGCGCAAGTGGTGGGAGGGGGGCCGGTGCCCCAAGGAGGAGGACC ATCGAGCTAAAGGTTTGGGCATGGAGAACATTGGTGGCATTTTTATCGTGCTCATCTGTGGCCTCATCAT TGCTGTCTTCGTGGCGGTCATGGAATTCATATGGTCCACACGGAGGTCAGCTGAGTCCGAGGAGACCCCA GCTCTGCATCCAGCTGCCTGCCAGTGTTCTGCACTTGGCCCAAGGACTCCTCTGAAGGAGCCGTCCATGC TGCTAGTAAAGGTGCCCTCCACCCGTGTCCAGGTTGCCTTCTCAAGGACATCCCTCCGGCAAGTCTGTCC TTTCCTCCTGCAGCATCAGTTGTCCTCTCTTTATTGGATTCAGGCCACCAACGTACAAATATGCTGTCAT TTCTCCAGTCTTAAACCCTCTCCTGACCTCACTTTCCCTCCCAGCCACCGCCCACTTTCTTCCCTTCTCT TTACAGCCTTGGCAGCAGTGGGGGGTCTCCCTGATGCTTCCAGTTTCTTCTTTCCTCCCATTTCCTCTTG CCCCCCACTCCAGTCTGGCATCGGTCCCTGCCACTCCACTGAAGCCACTCTTGTCACCAGCAACTTCCAT GTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239851 representing NM_001301030 Red=Cloning site Green=Tags(s) MPAELLLLLIVAFASPSCQVLSSLRMAAILDDQTVCGRGERLALALAREQINGIIEVPAKARVEVDIFEL QRDSQYETTDTMCQILPKGVVSVLGPSSSPASASTVSHICGEKEIPHIKVGPEETPRLQYLRFASVSLYP SNEDVSLAVSRILKSFNYPSASLICAKAECLLRLEELVRGFLISKETLSVRMLDDSRDPTPLLKEIRDDK VSTIIIDANASISHLILRKASELGMTSAFYKYILTTMDFPILHLDGIVEDSSNILGFSMFNTSHPFYPEF VRSLNMSWRENCEASTYLGPALSAALMFDAVHVVVSAVRELNRSQEIGVKPLACTSANIWPHGTSLMNYL RMVEYDGLTGRVEFNSKGQRTNYTLRILEKSRQGHREIGVWYSNRTLAMNATTLDINLSQTLANKTLVVT TILENPYVMRRPNFQALSGNERFEGFCVDMLRELAELLRFRYRLRLVEDGLYGAPEPNGSWTGMVGELIN RKADLAVAAFTITAEREKVIDFSKPFMTLGISILYRVHMGRKPGYFSFLDPFSPAVWLFMLLAYLAVSCV LFLAARLSPYEWYNPHPCLRARPHILENQYTLGNSLWFPVGGFMQQGSEIMPRALSTRCVSGVWWAFTLI IISSYTANLAAFLTVQRMEVPVESADDLADQTNIEYGTIHAGSTMTFFQNSRYQTYQRMWNYMQSKQPSV FVKSTEEGIARVLNSRYAFLLESTMNEYHRRLNCNLTQIGGLLDTKGYGIGMPLGSPFRDEITLAILQLQ ENNRLEILKRKWWEGGRCPKEEDHRAKGLGMENIGGIFIVLICGLIIAVFVAVMEFIWSTRRSAESEETP ALHPAACQCSALGPRTPLKEPSMLLVKVPSTRVQVAFSRTSLRQVCPFLLQHQLSSLYWIQATNVQICCH FSSLKPSPDLTFPPSHRPLSSLLFTALAAVGGLPDASSFFFPPISSCPPLQSGIGPCHSTEATLVTSNFH V TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001301030
ORF Size	2943 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001301030.1, NP_001287959.1
RefSeq Size	3331 bp
RefSeq ORF	2946 bp
Locus ID	2901
UniProt ID	Q16478
Protein Families	Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane
Protein Pathways	Neuroactive ligand-receptor interaction
MW	110.1 kDa
Gene Summary	This gene encodes a protein that belongs to the glutamate-gated ionic channel family. Glutamate functions as the major excitatory neurotransmitter in the central nervous system through activation of ligand-gated ion channels and G protein-coupled membrane receptors. The protein encoded by this gene forms functional heteromeric kainate-preferring ionic channels with the subunits encoded by related gene family members. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

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