SMARCA1 (NM_001282875) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	hSNF2L; ISWI; NURF140; SNF2L; SNF2L1; SNF2LB; SNF2LT; SWI; SWI2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239926 representing NM_001282875 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCAGGACACTGCCGCAGTGGCAGCCACCGTGGCAGCCGCGGATGCGACCGCCACTATCGTGGTCA TAGAGGACGAGCAGCCCGGGCCGTCCACCTCTCAGGAGGAGGGAGCGGCCGCCGCGGCCACCGAAGCCAC CGCGGCCACGGAGAAGGGCGAGAAGAAGAAGGAGAAAAACGTTTCTTCATTTCAACTCAAACTTGCTGCT AAAGCGCCTAAATCTGAAAAGGAAATGGACCCAGAATATGAAGAGAAAATGAAAGCCGACCGAGCAAAGA GATTTGAATTTTTACTGAAGCAGACAGAACTTTTTGCACATTTCATTCAGCCTTCAGCACAGAAATCTCC AACATCTCCACTGAACATGAAATTGGGACGTCCCCGAATAAAGAAAGATGAAAAGCAGAGCTTAATTTCT GCTGGAGACTACCGCCATAGGCGCACAGAGCAAGAAGAAGATGAAGAGCTACTGTCTGAGAGTCGGAAAA CATCTAATGTGTGTATTAGATTTGAGGTGTCACCTTCATATGTGAAAGGGGGGCCACTGAGAGATTATCA GATTCGAGGACTGAATTGGTTGATCTCTTTATATGAAAATGGAGTCAATGGCATTTTGGCTGATGAAATG GGCCTTGGGAAAACTTTACAAACAATTGCTTTGCTTGGTTACCTGAAACACTACCGAAATATTCCTGGAC CTCACATGGTTTTAGTTCCAAAGTCTACTTTACACAACTGGATGAATGAATTTAAACGATGGGTCCCATC TCTCCGTGTCATTTGTTTTGTCGGAGACAAGGATGCCAGAGCTGCTTTTATTCGTGATGAAATGATGCCA GGAGAGTGGGATGTTTGCGTTACTTCTTATGAGATGGTAATTAAAGAAAAATCTGTATTCAAAAAGTTTC ACTGGCGATACCTGGTCATTGATGAAGCTCACAGAATAAAGAATGAAAAATCTAAGCTTTCAGAGATTGT TCGTGAGTTCAAGTCGACTAACCGCTTGCTCCTAACTGGAACACCTTTGCAGAATAACCTGCATGAACTG TGGGCCTTACTCAACTTTTTATTGCCTGATGTCTTTAATTCTGCAGATGACTTTGATTCTTGGTTTGACA CTAAAAATTGTCTTGGTGATCAAAAACTCGTGGAAAGACTTCATGCAGTTTTAAAACCATTTTTGTTACG CCGTATAAAAACTGATGTAGAGAAGAGTCTGCCACCTAAAAAGGAAATAAAGATTTACTTGGGGCTGAGT AAGATGCAACGAGAATGGTATACAAAAATCCTGATGAAAGATATTGATGTTTTAAACTCTTCTGGCAAGA TGGACAAGATGCGACTCTTAAACATTCTGATGCAGCTTCGAAAGTGTTGTAATCATCCATATCTGTTTGA TGGTGCTGAACCTGGTCCACCTTATACCACTGATGAGCATATTGTCAGCAACAGTGGTAAAATGGTAGTT CTGGATAAACTATTGGCCAAACTCAAAGAACAGGGTTCAAGGGTTCTCATTTTCAGCCAGATGACTCGCT TGCTGGATATTTTGGAAGATTATTGCATGTGGCGTGGTTATGAGTATTGTCGACTGGATGGACAAACCCC GCATGAAGAAAGAGAGGAAGCAATAGAGGCTTTTAATGCTCCTAATAGTAGCAAATTCATCTTTATGCTA AGTACCAGGGCTGGAGGTCTCGGAATTAACCTGGCAAGTGCTGATGTGGTTATACTATATGATTCAGACT GGAACCCACAGGTTGATCTACAAGCTATGGATCGAGCACATCGTATTGGTCAGAAGAAACCAGTACGTGT ATTCCGTCTCATCACTGACAACACTGTTGAAGAGAGGATTGTAGAAAGAGCTGAGATAAAACTGAGACTC GATTCAATTGTTATACAACAAGGAAGACTCATTGACCAACAGTCTAACAAGCTGGCAAAAGAGGAAATGT TACAAATGATACGGCATGGAGCCACCCATGTTTTTGCTTCTAAAGAGAGTGAGTTGACAGATGAAGACAT TACAACTATTCTGGAAAGAGGGGAAAAGAAGACTGCAGAGATGAATGAACGCCTGCAAAAAATGGGAGAG TCTTCTCTAAGAAATTTTAGAATGGACATTGAACAAAGTTTATACAAATTTGAGGGAGAAGATTATAGAG AAAAACAGAAGCTTGGCATGGTGGAATGGATTGAACCTCCTAAACGAGAACGCAAAGCAAACTACGCAGT GGATGCCTACTTTAGAGAGGCTTTGCGTGTCAGCGAGCCAAAGATTCCAAAGGCTCCACGGCCTCCAAAA CAGCCAAATGTTCAGGATTTTCAATTTTTCCCACCACGCTTATTTGAGCTCCTGGAAAAGGAAATTCTTT ATTATCGGAAGACAATAGGCTATAAGGTTCCAAGGAATCCTGATATCCCAAATCCAGCTCTGGCTCAAAG AGAAGAGCAAAAAAAGATTGATGGAGCTGAACCTCTTACACCAGAAGAGACTGAAGAAAAGGAAAAACTT CTCACACAAGGTTTCACAAACTGGACTAAACGAGATTTTAACCAGTTTATTAAAGCTAATGAGAAATATG GAAGAGATGACATTGATAACATAGCTCGAGAGGTAGAGGGCAAATCCCCTGAGGAGGTCATGGAGTATTC AGCTGTATTTTGGGAACGTTGCAATGAATTACAGGACATTGAGAAAATTATGGCTCAAATTGAACGTGGA GAAGCAAGAATTCAACGAAGGATCAGTATCAAGAAAGCCCTGGATGCCAAAATTGCAAGATACAAGGCTC CATTTCATCAGTTGCGCATTCAGTATGGAACCAGCAAAGGAAAGAACTATACTGAGGAAGAAGATAGATT CTTGATTTGTATGTTACACAAAATGGGCTTTGATAGAGAAAATGTATATGAAGAATTAAGACAGTGTGTA CGAAATGCTCCCCAGTTTAGATTTGACTGGTTTATCAAGTCTAGGACTGCCATGGAATTCCAGAGACGCT GTAACACTCTGATTTCATTGATTGAGAAAGAAAATATGGAAATTGAGGAAAGAGAGAGAGCAGAAAAGAA GAAACGGGCAACTAAAACTCCAATGTCACAGAAAAGAAAAGCAGAGTCAGCTACTGAGAGCTCTGGAAAG AAGGATGTCAAGAAGGTGAAATCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239926 representing NM_001282875 Red=Cloning site Green=Tags(s) MEQDTAAVAATVAAADATATIVVIEDEQPGPSTSQEEGAAAAATEATAATEKGEKKKEKNVSSFQLKLAA KAPKSEKEMDPEYEEKMKADRAKRFEFLLKQTELFAHFIQPSAQKSPTSPLNMKLGRPRIKKDEKQSLIS AGDYRHRRTEQEEDEELLSESRKTSNVCIRFEVSPSYVKGGPLRDYQIRGLNWLISLYENGVNGILADEM GLGKTLQTIALLGYLKHYRNIPGPHMVLVPKSTLHNWMNEFKRWVPSLRVICFVGDKDARAAFIRDEMMP GEWDVCVTSYEMVIKEKSVFKKFHWRYLVIDEAHRIKNEKSKLSEIVREFKSTNRLLLTGTPLQNNLHEL WALLNFLLPDVFNSADDFDSWFDTKNCLGDQKLVERLHAVLKPFLLRRIKTDVEKSLPPKKEIKIYLGLS KMQREWYTKILMKDIDVLNSSGKMDKMRLLNILMQLRKCCNHPYLFDGAEPGPPYTTDEHIVSNSGKMVV LDKLLAKLKEQGSRVLIFSQMTRLLDILEDYCMWRGYEYCRLDGQTPHEEREEAIEAFNAPNSSKFIFML STRAGGLGINLASADVVILYDSDWNPQVDLQAMDRAHRIGQKKPVRVFRLITDNTVEERIVERAEIKLRL DSIVIQQGRLIDQQSNKLAKEEMLQMIRHGATHVFASKESELTDEDITTILERGEKKTAEMNERLQKMGE SSLRNFRMDIEQSLYKFEGEDYREKQKLGMVEWIEPPKRERKANYAVDAYFREALRVSEPKIPKAPRPPK QPNVQDFQFFPPRLFELLEKEILYYRKTIGYKVPRNPDIPNPALAQREEQKKIDGAEPLTPEETEEKEKL LTQGFTNWTKRDFNQFIKANEKYGRDDIDNIAREVEGKSPEEVMEYSAVFWERCNELQDIEKIMAQIERG EARIQRRISIKKALDAKIARYKAPFHQLRIQYGTSKGKNYTEEEDRFLICMLHKMGFDRENVYEELRQCV RNAPQFRFDWFIKSRTAMEFQRRCNTLISLIEKENMEIEERERAEKKKRATKTPMSQKRKAESATESSGK KDVKKVKS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282875
ORF Size	3174 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282875.2
RefSeq Size	3989 bp
RefSeq ORF	3177 bp
Locus ID	6594
UniProt ID	P28370
Protein Families	Transcription Factors
MW	123.3 kDa
Gene Summary	This gene encodes a member of the SWI/SNF family of proteins. The encoded protein is an ATPase which is expressed in diverse tissues and contributes to the chromatin remodeling complex that is involved in transcription. The protein may also play a role in DNA damage, growth inhibition and apoptosis of cancer cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.