PRKCBP1 (ZMYND8) (NM_001281773) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	PRKCBP1; PRO2893; RACK7
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC240048 representing NM_001281773 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATATCTCTACTCGCTCCAAAGATCCTGGCTCTGCAGAGAGAACAGCCCAGAAAAGAAAGTTCCCCA GCCCTCCACATTCTTCCAATGGCCACTCGCCGCAGGACACATCAACAAGCCCCATTAAAAAGAAAAAGAA ACCTGGCTTACTGAACAGTAACAATAAGGAGCAGTCAGAACTAAGACATGGTCCGTTTTACTATATGAAG CAGCCACTCACCACAGACCCTGTTGATGTTGTACCGCAGGATGGACGGAATGATTTCTACTGCTGGGTTT GTCACCGGGAAGGCCAAGTCCTTTGCTGTGAGCTCTGTCCCCGGGTTTATCACGCTAAGTGTCTGAGACT GACATCGGAACCAGAGGGGGACTGGTTTTGTCCTGAATGTGAGAAAATTACAGTAGCAGAATGCATCGAG ACCCAGAGTAAAGCCATGACAATGCTCACCATTGAACAGTTATCCTACCTGCTCAAGTTTGCCATTCAGA AAATGAAACAGCCAGGGACAGATGCATTCCAGAAGCCCGTTCCATTGGAACAGCACCCTGACTATGCGGA ATACATCTTCCATCCAATGGACCTTTGTACATTGGAAAAGAATGCGAAAAAGAAAATGTATGGCTGCACA GAAGCCTTCCTGGCTGATGCAAAGTGGATTTTGCACAACTGCATCATTTATAATGGGGGAAATCACAAAT TGACGCAAATAGCGAAAGTAGTCATCAAAATCTGTGAACATGAGATGAATGAAATCGAAGTATGTCCAGA ATGTTATCTAGCTGCTTGCCAAAAACGAGATAACTGGTTTTGTGAGCCTTGTAGCAATCCACATCCTTTG GTCTGGGCCAAACTGAAGGGGTTTCCATTCTGGCCTGCAAAAGCTCTAAGGGATAAAGACGGGCAGGTCG ATGCCCGATTCTTTGGACAACATGACAGGGCCTGGGTTCCAATAAATAATTGCTACCTCATGTCTAAAGA AATTCCTTTTTCTGTGAAAAAGACTAAGAGCATCTTCAACAGTGCCATGCAAGAGATGGAGGTTTACGTG GAGAACATCCGCAGGAAGTTTGGGGTTTTTAATTACTCTCCATTTAGGACACCCTACACACCCAACAGCC AGTATCAAATGCTGCTCGATCCCACCAACCCCAGCGCCGGCACTGCCAAGATAGACAAGCAGGAGAAGGT CAAGCTCAACTTTGACATGACGGCATCCCCCAAGATCCTGATGAGCAAGCCTGTGCTGAGTGGGGGCACA GGCCGCCGGATTTCCTTGTCGGATATGCCGCGCTCCCCCATGAGCACAAACTCTTCTGTGCACACGGGCT CCGACGTGGAGCAGGATGCTGAGAAGAAGGCCACGTCGAGCCACTTCAGTGCGAGCGAGGAGTCCATGGA CTTCCTGGATAAGAGCACAGCTTCACCAGCCTCCACCAAGACGGGACAAGCAGGGAGTTTATCCGGCAGC CCAAAGCCCTTCTCTCCTCAACTGTCAGCTCCTATCACGACGAAAACGGACAAAACCTCCACCACCGGCA GCATCCTGAATCTTAACCTGGATCGAAGCAAAGCTGAGATGGATTTGAAGGAGCTGAGCGAGTCGGTCCA GCAACAGTCCACCCCTGTTCCTCTCATCTCTCCCAAGCGCCAGATTCGTAGCAGGTTCCAGCTGAATCTT GACAAGACCATAGAGAGTTGCAAAGCACAATTAGGCATAAATGAAATCTCGGAAGATGTCTATACGGCCG TAGAGCACAGCGATTCGGAGGATTCTGAGAAGTCAGATAGTAGCGATAGTGAGTATATCAGTGATGATGA GCAGAAGTCTAAGAACGAGCCAGAAGACACAGAGGACAAAGAAGGTTGTCAGATGGACAAAGAGCCATCT GCTGTTAAAAAAAAGCCCAAGCCTACAAACCCAGTGGAGATTAAAGAGGAGCTGAAAAGCACGTCACCAG CCAGCGAGAAGGCAGACCCTGGAGCAGTCAAGGACAAGGCCAGCCCTGAGCCTGAGAAGGACTTTTCCGA AAAGGCAAAACCTTCACCTCACCCCATAAAGGATAAACTGAAGGGAAAAGATGAGACGGATTCCCCAACA GTCCATTTGGGCCTGGACTCTGATTCAGAGAGCGAACTTGTCATAGATTTAGGAGAAGACCATTCTGGGC GGGAGGGTCGAAAAAATAAGAAGGAACCCAAAGAACCATCTCCCAAACAGGATGTTGTAGGTAAAACTCC ACCATCCACGACGGTGGGCAGCCATTCTCCCCCGGAAACACCGGTGCTCACCCGCTCTTCCGCCCAAACT TCCGCGGCTGGCGCCACAGCCACCACCAGCACGTCCTCCACGGTCACCGTCACGGCCCCGGCCCCCGCCG CCACAGGAAGCCCAGTGAAAAAGCAGAGGCCGCTTTTACCGAAGGAGACTGCCCCGGCCGTGCAGCGGGT CGTGTGGAACTCATCAAGTAAGTTTCAAACGTCCTCCCAAAAGTGGCACATGCAGAAGATGCAGCGTCAG CAGCAGCAGCAGCAGCAGCAAAACCAGCAGCAGCAGCCTCAGTCTTCCCAGGGGACGAGATATCAGACCA GACAGGCTGTGAAAGCTGTCCAGCAGAAGGAGATCACACAGAGCCCATCCACGTCCACCATCACCCTGGT GACCAGCACACAGTCATCGCCCCTGGTCACCAGCTCGGGGTCCATGAGCACCCTTGTGTCCTCAGTCAAC GCTGACCTGCCCATCGCCACTGCCTCAGCTGATGTCGCCGCTGATATTGCCAAGTACACTAGCAAAATGA TGGATGCAATAAAAGGAACAATGACAGAAATATACAACGATCTTTCTAAAAACACTACTGGAAGCACAAT AGCTGAGATTCGCAGGCTGAGGATCGAGATAGAGAAGCTCCAGTGGCTGCACCAGCAAGAGCTCTCCGAA ATGAAACACAACTTAGAGCTGACCATGGCGGAGATGCGGCAGAGCCTGGAGCAGGAGCGGGACCGGCTCA TCGCCGAGGTGAAGAAGCAGCTGGAGTTGGAGAAGCAGCAGGCGGTGGATGAGACCAAGAAGAAGCAGTG GTGCGCCAACTGCAAGAAGGAGGCCATCTTTTACTGCTGTTGGAACACCAGCTACTGTGACTACCCCTGC CAGCAAGCCCACTGGCCTGAGCACATGAAGTCCTGCACCCAGTCAGCTACTGCTCCTCAGCAGGAAGCGG ATGCTGAGGTGAACACAGAAACACTAAATAAGTCCTCCCAGGGGAGCTCCTCGAGCACACAATCAGCACC TTCAGAAACGGCCAGCGCCTCCAAAGAGAAGGAGACGTCAGCTGAGAAAAGCAAGGAGAGTGGCTCGACC CTTGACCTTTCTGGCTCCAGAGAGACGCCCTCCTCCATTCTCTTAGGCTCCAACCAAGGCTCTGTTAGCA AAAGGTGTGACAAGCAACCTGCCTATGCCCCAACCACCACAGACCACCAGCCGCACCCCAACTACCCCGC CCAGAAGTACCATTCCCGGAGTAATAAATCCAGTTGGAGCAGCAGTGATGAGAAGAGGGGATCGACACGT TCCGATCACAACACCAGTACCAGCACGAAGAGCCTCCTCCCGAAAGAGTCTCGGCTGGACACCTTCTGGG AC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC240048 representing NM_001281773 Red=Cloning site Green=Tags(s) MDISTRSKDPGSAERTAQKRKFPSPPHSSNGHSPQDTSTSPIKKKKKPGLLNSNNKEQSELRHGPFYYMK QPLTTDPVDVVPQDGRNDFYCWVCHREGQVLCCELCPRVYHAKCLRLTSEPEGDWFCPECEKITVAECIE TQSKAMTMLTIEQLSYLLKFAIQKMKQPGTDAFQKPVPLEQHPDYAEYIFHPMDLCTLEKNAKKKMYGCT EAFLADAKWILHNCIIYNGGNHKLTQIAKVVIKICEHEMNEIEVCPECYLAACQKRDNWFCEPCSNPHPL VWAKLKGFPFWPAKALRDKDGQVDARFFGQHDRAWVPINNCYLMSKEIPFSVKKTKSIFNSAMQEMEVYV ENIRRKFGVFNYSPFRTPYTPNSQYQMLLDPTNPSAGTAKIDKQEKVKLNFDMTASPKILMSKPVLSGGT GRRISLSDMPRSPMSTNSSVHTGSDVEQDAEKKATSSHFSASEESMDFLDKSTASPASTKTGQAGSLSGS PKPFSPQLSAPITTKTDKTSTTGSILNLNLDRSKAEMDLKELSESVQQQSTPVPLISPKRQIRSRFQLNL DKTIESCKAQLGINEISEDVYTAVEHSDSEDSEKSDSSDSEYISDDEQKSKNEPEDTEDKEGCQMDKEPS AVKKKPKPTNPVEIKEELKSTSPASEKADPGAVKDKASPEPEKDFSEKAKPSPHPIKDKLKGKDETDSPT VHLGLDSDSESELVIDLGEDHSGREGRKNKKEPKEPSPKQDVVGKTPPSTTVGSHSPPETPVLTRSSAQT SAAGATATTSTSSTVTVTAPAPAATGSPVKKQRPLLPKETAPAVQRVVWNSSSKFQTSSQKWHMQKMQRQ QQQQQQQNQQQQPQSSQGTRYQTRQAVKAVQQKEITQSPSTSTITLVTSTQSSPLVTSSGSMSTLVSSVN ADLPIATASADVAADIAKYTSKMMDAIKGTMTEIYNDLSKNTTGSTIAEIRRLRIEIEKLQWLHQQELSE MKHNLELTMAEMRQSLEQERDRLIAEVKKQLELEKQQAVDETKKKQWCANCKKEAIFYCCWNTSYCDYPC QQAHWPEHMKSCTQSATAPQQEADAEVNTETLNKSSQGSSSSTQSAPSETASASKEKETSAEKSKESGST LDLSGSRETPSSILLGSNQGSVSKRCDKQPAYAPTTTDHQPHPNYPAQKYHSRSNKSSWSSSDEKRGSTR SDHNTSTSTKSLLPKESRLDTFWD TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001281773
ORF Size	3642 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001281773.2, NP_001268702.1
RefSeq Size	5542 bp
RefSeq ORF	3645 bp
Locus ID	23613
UniProt ID	Q9ULU4
Protein Families	Druggable Genome, Transcription Factors
MW	135.4 kDa
Gene Summary	The protein encoded by this gene is a receptor for activated C-kinase (RACK) protein. The encoded protein has been shown to bind in vitro to activated protein kinase C beta I. In addition, this protein is a cutaneous T-cell lymphoma-associated antigen. Finally, the protein contains a bromodomain and two zinc fingers, and is thought to be a transcriptional regulator. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

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