TRPS1 (NM_001282902) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	GC79; LGCR
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC240094 representing NM_001282902 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGAGTAATATGGTCCGGAAAAAGAACCCCCCTCTGAGAAACGTTGCAAGTGAAGGCGAGGGCCAGA TCCTGGAGCCTATAGGTACAGAAAGCAAGGTATCTGGAAAGAACAAAGAATTTTCTGCAGATCAGATGTC AGAAAATACGGATCAGAGTGATGCTGCAGAACTAAATCATAAGGAGGAACATAGCTTGCATGTTCAAGAT CCATCTTCTAGCAGTAAGAAGGACTTGAAAAGCGCAGTTCTGAGTGAGAAGGCTGGCTTCAATTATGAAA GCCCCAGTAAGGGAGGAAACTTTCCCTCCTTTCCGCATGATGAGGTGACAGACAGAAATATGTTGGCTTT CTCATCTCCAGCTGCTGGGGGAGTCTGTGAGCCCTTGAAGTCTCCGCAAAGAGCAGAGGCAGATGACCCT CAAGATATGGCCTGCACCCCCTCAGGGGACTCACTGGAGACAAAGGAAGATCAGAAGATGTCACCAAAGG CTACAGAGGAAACAGGGCAAGCACAGAGTGGTCAAGCCAATTGTCAAGGTTTGAGCCCAGTTTCAGTGGC CTCAAAAAACCCACAAGTGCCTTCAGATGGGGGTGTAAGACTGAATAAATCCAAAACTGACTTACTGGTG AATGACAACCCAGACCCGGCACCTCTGTCTCCAGAGCTTCAGGACTTTAAATGCAATATCTGTGGATATG GTTACTACGGCAACGACCCCACAGATCTGATTAAGCACTTCCGAAAGTATCACTTAGGACTGCATAACCG CACCAGGCAAGATGCTGAGCTGGACAGCAAAATCTTGGCCCTTCATAACATGGTGCAGTTCAGCCATTCC AAAGACTTCCAGAAGGTCAACCGTTCTGTGTTTTCTGGTGTGCTGCAGGACATCAATTCTTCAAGGCCTG TTTTACTAAATGGGACCTATGATGTGCAGGTGACTTCAGGTGGAACATTCATTGGCATTGGACGGAAAAC ACCAGATTGCCAAGGGAACACCAAGTATTTCCGCTGTAAATTCTGCAATTTCACTTATATGGGCAACTCA TCCACCGAATTAGAACAACATTTTCTTCAGACTCACCCAAACAAAATAAAAGCTTCTCTCCCCTCCTCTG AGGTTGCAAAACCTTCAGAGAAAAACTCTAACAAGTCCATCCCTGCACTTCAATCCAGTGATTCTGGAGA CTTGGGAAAATGGCAGGACAAGATAACAGTCAAAGCAGGAGATGACACTCCTGTTGGGTACTCAGTGCCC ATAAAGCCCCTCGATTCCTCTAGACAAAATGGTACAGAGGCCACCAGTTACTACTGGTGTAAATTTTGTA GTTTCAGCTGTGAGTCATCTAGCTCACTTAAACTGCTAGAACATTATGGCAAGCAGCACGGAGCAGTGCA GTCAGGCGGCCTTAATCCAGAGTTAAATGATAAGCTTTCCAGGGGCTCTGTCATTAATCAGAATGATCTA GCCAAAAGTTCAGAAGGAGAGACAATGACCAAGACAGACAAGAGCTCGAGTGGGGCTAAAAAGAAGGACT TCTCCAGCAAGGGAGCCGAGGATAATATGGTAACGAGCTATAATTGTCAGTTCTGTGACTTCCGATATTC CAAAAGCCATGGCCCTGATGTAATTGTAGTGGGGCCACTTCTCCGTCATTATCAACAGCTCCATAACATT CACAAGTGTACCATTAAACACTGTCCATTCTGTCCCAGAGGACTTTGCAGCCCAGAAAAGCACCTTGGAG AAATTACTTATCCGTTTGCTTGTAGAAAAAGTAATTGTTCCCACTGTGCACTCTTGCTTCTGCACTTGTC TCCTGGGGCGGCTGGAAGCTCGCGAGTCAAACATCAGTGCCATCAGTGTTCATTCACCACCCCTGACGTA GATGTACTCCTCTTTCACTATGAAAGTGTGCATGAGTCCCAAGCATCGGATGTCAAACAAGAAGCAAATC ACCTGCAAGGATCGGATGGGCAGCAGTCTGTCAAGGAAAGCAAAGAACACTCATGTACCAAATGTGATTT TATTACCCAAGTGGAAGAAGAGATTTCCCGACACTACAGGAGAGCACACAGCTGCTACAAATGCCGTCAG TGCAGTTTTACAGCTGCCGATACTCAGTCACTACTGGAGCACTTCAACACTGTTCACTGCCAGGAACAGG ACATCACTACAGCCAACGGCGAAGAGGACGGTCATGCCATATCCACCATCAAAGAGGAGCCCAAAATTGA CTTCAGGGTCTACAATCTGCTAACTCCAGACTCTAAAATGGGAGAGCCAGTTTCTGAGAGTGTGGTGAAG AGAGAGAAGCTGGAAGAGAAGGACGGGCTCAAAGAGAAAGTTTGGACCGAGAGTTCCAGTGATGACCTTC GCAATGTGACTTGGAGAGGGGCAGACATCCTGCGGGGGAGTCCGTCATACACCCAAGCAAGCCTGGGGCT GCTGACGCCTGTGTCTGGCACCCAAGAGCAGACAAAGACTCTAAGGGATAGTCCCAATGTGGAGGCCGCC CATCTGGCGCGACCTATTTATGGCTTGGCTGTGGAAACCAAGGGATTCCTGCAGGGGGCGCCAGCTGGCG GAGAGAAGTCTGGGGCCCTCCCCCAGCAGTATCCTGCATCGGGAGAAAACAAGTCCAAGGATGAATCCCA GTCCCTGTTACGGAGGCGTAGAGGCTCCGGTGTTTTTTGTGCCAATTGCCTGACCACAAAGACCTCTCTC TGGCGAAAGAATGCAAATGGCGGATATGTATGCAACGCGTGTGGCCTCTACCAGAAGCTTCACTCGACTC CCAGGCCTTTAAACATCATTAAACAAAACAACGGTGAGCAGATTATTAGGAGGAGAACAAGAAAGCGCCT TAACCCAGAGGCACTTCAGGCTGAGCAGCTCAACAAACAGCAGAGGGGCAGCAATGAGGAGCAAGTCAAT GGAAGCCCGTTAGAGAGGAGGTCAGAAGATCATCTAACTGAAAGTCACCAGAGAGAAATTCCACTCCCCA GCCTAAGTAAATACGAAGCCCAGGGTTCATTGACTAAAAGCCATTCTGCTCAGCAGCCAGTCCTGGTCAG CCAAACTCTGGATATTCACAAAAGGATGCAACCTTTGCACATTCAGATAAAAAGTCCTCAGGAAAGTACT GGAGATCCAGGAAATAGTTCATCCGTATCTGAAGGGAAAGGAAGTTCTGAGAGAGGCAGTCCTATAGAAA AGTACATGAGACCTGCGAAACACCCAAATTATTCACCACCAGGCAGCCCTATTGAAAAGTACCAGTACCC ACTTTTTGGACTTCCCTTTGTACATAATGACTTCCAGAGTGAAGCTGATTGGCTGCGGTTCTGGAGTAAA TATAAGCTCTCCGTTCCTGGGAATCCGCACTACTTGAGTCACGTGCCTGGCCTACCAAATCCTTGCCAAA ACTATGTGCCTTATCCCACCTTCAATCTGCCTCCTCATTTTTCAGCTGTTGGATCAGACAATGACATTCC TCTAGATTTGGCGATCAAGCATTCCAGACCTGGGCCAACTGCAAACGGTGCCTCCAAGGAGAAAACGAAG GCACCACCAAATGTAAAAAATGAAGGTCCCTTGAATGTAGTAAAAACAGAGAAAGTTGATAGAAGTACTC AAGATGAACTTTCAACAAAATGTGTGCACTGTGGCATTGTCTTTCTGGATGAAGTGATGTATGCTTTGCA TATGAGTTGCCATGGTGACAGTGGACCTTTCCAGTGCAGCATATGCCAGCATCTTTGCACGGACAAATAT GACTTCACAACACATATCCAGAGGGGCCTGCATAGGAACAATGCACAAGTGGAAAAAAATGGAAAACCTA AAGAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC240094 representing NM_001282902 Red=Cloning site Green=Tags(s) MQSNMVRKKNPPLRNVASEGEGQILEPIGTESKVSGKNKEFSADQMSENTDQSDAAELNHKEEHSLHVQD PSSSSKKDLKSAVLSEKAGFNYESPSKGGNFPSFPHDEVTDRNMLAFSSPAAGGVCEPLKSPQRAEADDP QDMACTPSGDSLETKEDQKMSPKATEETGQAQSGQANCQGLSPVSVASKNPQVPSDGGVRLNKSKTDLLV NDNPDPAPLSPELQDFKCNICGYGYYGNDPTDLIKHFRKYHLGLHNRTRQDAELDSKILALHNMVQFSHS KDFQKVNRSVFSGVLQDINSSRPVLLNGTYDVQVTSGGTFIGIGRKTPDCQGNTKYFRCKFCNFTYMGNS STELEQHFLQTHPNKIKASLPSSEVAKPSEKNSNKSIPALQSSDSGDLGKWQDKITVKAGDDTPVGYSVP IKPLDSSRQNGTEATSYYWCKFCSFSCESSSSLKLLEHYGKQHGAVQSGGLNPELNDKLSRGSVINQNDL AKSSEGETMTKTDKSSSGAKKKDFSSKGAEDNMVTSYNCQFCDFRYSKSHGPDVIVVGPLLRHYQQLHNI HKCTIKHCPFCPRGLCSPEKHLGEITYPFACRKSNCSHCALLLLHLSPGAAGSSRVKHQCHQCSFTTPDV DVLLFHYESVHESQASDVKQEANHLQGSDGQQSVKESKEHSCTKCDFITQVEEEISRHYRRAHSCYKCRQ CSFTAADTQSLLEHFNTVHCQEQDITTANGEEDGHAISTIKEEPKIDFRVYNLLTPDSKMGEPVSESVVK REKLEEKDGLKEKVWTESSSDDLRNVTWRGADILRGSPSYTQASLGLLTPVSGTQEQTKTLRDSPNVEAA HLARPIYGLAVETKGFLQGAPAGGEKSGALPQQYPASGENKSKDESQSLLRRRRGSGVFCANCLTTKTSL WRKNANGGYVCNACGLYQKLHSTPRPLNIIKQNNGEQIIRRRTRKRLNPEALQAEQLNKQQRGSNEEQVN GSPLERRSEDHLTESHQREIPLPSLSKYEAQGSLTKSHSAQQPVLVSQTLDIHKRMQPLHIQIKSPQEST GDPGNSSSVSEGKGSSERGSPIEKYMRPAKHPNYSPPGSPIEKYQYPLFGLPFVHNDFQSEADWLRFWSK YKLSVPGNPHYLSHVPGLPNPCQNYVPYPTFNLPPHFSAVGSDNDIPLDLAIKHSRPGPTANGASKEKTK APPNVKNEGPLNVVKTEKVDRSTQDELSTKCVHCGIVFLDEVMYALHMSCHGDSGPFQCSICQHLCTDKY DFTTHIQRGLHRNNAQVEKNGKPKE SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282902
ORF Size	3855 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282902.3
RefSeq Size	9514 bp
RefSeq ORF	3858 bp
Locus ID	7227
UniProt ID	Q9UHF7
Protein Families	Druggable Genome, Transcription Factors
MW	142.4 kDa
Gene Summary	This gene encodes a transcription factor that represses GATA-regulated genes and binds to a dynein light chain protein. Binding of the encoded protein to the dynein light chain protein affects binding to GATA consensus sequences and suppresses its transcriptional activity. Defects in this gene are a cause of tricho-rhino-phalangeal syndrome (TRPS) types I-III. [provided by RefSeq, Jul 2008]

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