l Afadin (AFDN) (NM_001291964) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AF6; l-afadin; MLL-AF6; MLLT4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC240207 representing NM_001291964 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGATTTTATTTTCAAGATAAAGCTGCTGGAAACTTTGCAACAAAATGTATTCGGGTCTCTAGTACTG CCACCACTCAAGATGTAATCGAAACGCTCGCGGAGAAATTTCGACCTGATATGCGAATGCTGTCCTCTCC CAAGTATTCACTCTATGAAGTGCATGTCAGCGGAGAAGAAAGAAGATTGGATATAGATGAGAAACCTCTA GTTGTACAACTGAATTGGAACAAAGATGATCGGGAAGGCAGATTTGTTCTTAAGAATGAGAATGACGCCA TTCCTCCTAAGAAGGCTCAAAGTAATGGACCTGAAAAGCAGGAAAAAGAAGGGGTTATCCAGAACTTCAA GAGAACTCTCTCAAAGAAAGAAAAGAAGGAAAAAAAGAAGAGAGAAAAAGAGGCATTGCGACAGGCATCT GATAAAGATGATAGACCTTTCCAAGGGGAGGATGTTGAAAATTCTCGACTGGCTGCTGAGGTTTACAAAG ACATGCCGGAAACCAGCTTTACTCGAACCATTTCTAATCCTGAGGTGGTTATGAAACGACGGAGGCAGCA AAAATTGGAAAAGAGAATGCAGGAATTTCGGAGCTCAGATGGGCGGCCTGATTCAGGTGGAACATTGAGA ATTTATGCAGATAGTTTAAAACCAAATATTCCCTACAAGACAATCCTGCTGTCTACTACAGATCCTGCAG ACTTTGCTGTGGCTGAAGCTTTAGAGAAGTATGGTCTGGAAAAAGAAAACCCTAAGGATTACTGCATCGC CCGGGTTATGCTTCCTCCTGGAGCCCAGCATTCTGATGAAAAGGGTGCTAAAGAAATTATTCTTGATGAT GATGAGTGTCCTTTACAAATCTTCAGGGAATGGCCAAGTGACAAAGGGATTTTAGTCTTTCAGTTGAAGA GGAGGCCACCAGACCACATCCCAAAGAAAACCAAGAAACACTTGGAAGGCAAGACACCCAAGGGAAAGGA GAGAGCTGACGGGTCTGGCTATGGCTCCACCCTTCCTCCGGAGAAGCTGCCCTATTTAGTAGAGTTAAGC CCAGGGAGAAGGAATCACTTTGCCTACTACAACTATCACACTTACGAAGATGGTTCTGACTCTAGAGATA AGCCAAAGCTTTACCGCCTTCAGTTAAGTGTTACTGAAGTTGGGACAGAAAAGTTGGATGACAACTCTAT CCAGTTGTTTGGCCCAGGAATTCAGCCCCATCACTGTGACCTTACCAACATGGATGGAGTGGTCACTGTG ACGCCCAGAAGTATGGACGCAGAAACCTACGTGGAAGGCCAGCGCATCTCAGAAACCACCATGCTGCAGA GTGGCATGAAAGTGCAGTTTGGGGCGTCCCATGTATTTAAGTTTGTGGACCCCAGTCAGGATCATGCTCT TGCAAAAAGATCTGTGGATGGAGGCCTGATGGTTAAGGGCCCAAGACATAAACCTGGAATTGTTCAGGAG ACAACTTTTGATTTGGGAGGAGATATTCATAGTGGGACAGCATTACCGACAAGCAAGAGCACCACTAGGC TGGACAGCGACAGAGTGTCGTCTGCCTCTAGCACAGCCGAGCGGGGAATGGTGAAGCCGATGATCAGAGT AGAACAGCAGCCAGATTATCGCAGGCAAGAAAGCAGAACACAGGATGCTTCTGGGCCTGAGCTGATACTA CCTGCAAGCATTGAATTCAGGGAAAGTTCTGAAGATTCATTTTTGTCTGCCATTATAAATTATACTAATA GCTCTACAGTCCACTTTAAGTTGTCCCCTACATATGTATTATATATGGCATGCCGGTATGTATTGTCCAA CCAGTACAGACCTGACATCAGCCCTACAGAGCGCACACATAAAGTCATTGCAGTCGTCAACAAGATGGTG AGCATGATGGAGGGTGTCATCCAGAAACAGAAGAATATTGCAGGGGCACTTGCCTTCTGGATGGCAAATG CATCTGAACTTCTCAACTTCATTAAGCAAGACCGAGACCTTAGTCGGATCACACTGGATGCTCAAGATGT TTTAGCACATTTGGTTCAAATGGCATTTAAATACTTGGTTCACTGTCTTCAATCAGAACTTAATAATTAC ATGCCAGCCTTTCTAGATGACCCTGAAGAGAACAGTCTGCAACGACCAAAAATAGATGATGTGCTGCACA CGCTCACAGGAGCCATGTCCTTGCTACGACGCTGCAGAGTCAATGCCGCCCTGACCATCCAGCTCTTCTC TCAGCTCTTCCACTTCATCAATATGTGGCTGTTCAATAGATTGGTGACCGACCCAGATTCGGGGCTGTGC TCCCATTACTGGGGTGCGATTATCCGTCAGCAGTTGGGCCATATTGAAGCCTGGGCTGAGAAGCAGGGGC TGGAACTGGCTGCGGACTGTCATCTGAGCAGGATCGTGCAGGCAACGACTTTGCTTACCATGGATAAGTA TGCACCTGATGACATTCCAAATATAAACAGCACCTGCTTTAAGTTAAATTCATTACAACTTCAAGCCTTA TTACAGAACTATCACTGTGCACCTGATGAGCCTTTTATCCCAACGGATCTTATAGAAAATGTAGTGACTG TGGCTGAAAACACTGCCGATGAGCTGGCCCGCAGTGATGGAAGGGAAGTGCAGTTGGAGGAGGATCCTGA TCTGCAGCTGCCGTTTCTTTTGCCAGAAGATGGTTATTCTTGTGATGTTGTCAGAAACATTCCAAATGGT TTACAAGAATTTTTAGACCCTCTGTGCCAGAGAGGATTTTGCAGGTTAATTCCTCACACACGTTCACCAG GTACTTGGACAATATATTTTGAAGGTGCAGATTATGAAAGTCACCTTCTGCGTGAGAACACAGAGCTGGC TCAGCCTCTGAGGAAAGAACCTGAAATAATCACTGTGACCCTAAAAAAGCAGAATGGAATGGGCCTTAGC ATTGTTGCAGCAAAGGGTGCTGGTCAAGATAAACTAGGAATCTATGTGAAGTCGGTTGTGAAAGGAGGTG CTGCAGATGTGGATGGACGTCTAGCTGCAGGTGATCAGCTCCTCAGTGTGGATGGACGAAGTCTGGTTGG ACTCTCTCAGGAAAGGGCGGCAGAACTCATGACAAGAACAAGCTCTGTGGTGACACTGGAAGTAGCAAAG CAGGGTGCCATCTACCACGGTCTGGCCACCCTTCTCAATCAGCCATCCCCCATGATGCAGAGAATTTCAG ATCGTCGTGGCTCAGGTAAACCCCGACCAAAGAGTGAAGGCTTTGAGCTCTATAATAATTCAACTCAAAA TGGGTCTCCTGAGAGTCCTCAGCTGCCTTGGGCAGAATATAGTGAACCAAAGAAATTGCCTGGTGATGAC AGACTGATGAAAAATAGAGCTGATCACCGTTCCAGCCCCAACGTAGCAAATCAGCCTCCTAGTCCTGGAG GGAAAAGTGCATATGCCTCTGGAACAACAGCGAAGATAACATCTGTCTCTACTGGAAACCTCTGCACTGA GGAGCAGACGCCTCCGCCTAGACCTGAAGCCTACCCCATCCCCACTCAGACGTACACCAGAGAGTATTTT ACCTTCCCAGCTTCCAAATCCCAGGATCGGATGGCTCCTCCTCAGAACCAGTGGCCAAATTATGAGGAAA AGCCACATATGCACACAGATAGTAATCATTCCAGTATTGCAATTCAGCGTGTTACACGTTCCCAAGAAGA ACTTCGAGAAGATAAAGCTTACCAACTTGAGCGGCATCGAATAGAGGCAGCTATGGACCGAAAGTCTGAT AGTGATATGTGGATAAATCAGAGCTCCTCACTGGACTCCAGTACCTCTAGCCAGGAGCATCTGAACCATT CCTCTAAGTCGGTCACCCCTGCTTCCACACTGACCAAAAGTGGCCCTGGCCGTTGGAAAACACCAGCAGC CATACCGGCCACCCCTGTGGCCGTCTCCCAGCCAATCCGAACAGACCTGCCTCCGCCACCCCCGCCACCT CCAGTCCACTATGCCGGTGATTTCGATGGAATGTCCATGGATTTGCCTCTCCCACCACCCCCTTCCGCCA ACCAGATAGGGCTGCCGTCTGCGCAGGTGGCTGCTGCTGAACGGAGAAAGAGAGAAGAACATCAGCGTTG GTATGAGAAGGAGAAGGCCCGCCTGGAGGAGGAGCGGGAGAGGAAGCGGAGAGAGCAGGAGAGGAAGTTG GGCCAGATGCGCACTCAGTCCTTAAACCCTGCTCCGTTTTCTCCCCTGACTGCACAGCAGATGAAGCCCG AAAAGCCTTCCACACTCCAGCGGCCACAGGAAACAGTCATTCGGGAGCTGCAGCCTCAGCAGCAGCCCCG CACGATCGAGCGCAGAGACTTGCAGTACATTACAGTCAGCAAAGAGGAGCTTTCCTCGGGGGACAGTCTG TCCCCCGACCCGTGGAAGCGGGACGCCAAGGAGAAGCTGGAGAAGCAGCAGCAGATGCACATCGTGGACA TGCTGAGCAAGGAGATCCAGGAGCTCCAGAGCAAACCGGACCGCAGCGCCGAGGAGAGCGACCGGCTGCG CAAGCTCATGCTGGAGTGGCAGTTCCAGAAGAGACTCCAGGAGTCGAAGCAGAAGGACGAAGATGACGAG GAGGAGGAGGACGATGATGTGGACACCATGCTGATCATGCAGCGCCTGGAGGCTGAACGAAGAGCGAGGG ACGAGGAGCGGAGGCGGCAGCAGCAGTTAGAAGAGATGCGCAAGCGGGAAGCGGAAGACCGAGCGAGGCA AGAGGAAGAGCGCCGGCGGCAGGAGGAGGAGCGAACAAAACGAGACGCTGAAGAAAAGAGGCGACAGGAA GAAGGGTATTACAGCCGCCTGGAAGCCGAGAGGCGCAGACAGCACGACGAGGCGGCGCGCAGGTTGCTGG AGCCCGAGGCGCCCGGTCTGTGCCGCCCTCCGCTTCCCCGGGACTACGAGCCCCCGTCCCCGTCCCCCGC GCCCGGCGCCCCTCCTCCCCCGCCTCAGCGAAACGCCTCCTACCTCAAAACACAGGTCCTCTCCCCCGAC TCGCTGTTCACTGCCAAGTTTGTTGCATACAATGAGGAGGAGGAGGAGGAGGACTGCAGCCTAGCAGGAC CAAACTCTTACCCAGGATCTACTGGAGCAGCTGTTGGAGCCCATGACGCCTGTCGGGATGCAAAAGAGAA GCGCTCTAAAAGCCAAGATGCAGATTCACCTGGAAGTTCTGGGGCCCCTGAAAACTTGACATTCAAGGAA CGCCAGCGTCTTTTTTCACAAGGTCAAGATGTATCCAATAAAGTGAAAGCTTCTCGTAAATTAACAGAAC TGGAGAATGAACTGAACACAAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC240207 representing NM_001291964 Red=Cloning site Green=Tags(s) MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPL VVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQAS DKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLR IYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDD DECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELS PGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTV TPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQE TTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPELIL PASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMV SMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNY MPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLC SHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQAL LQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNG LQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLS IVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAK QGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDD RLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYF TFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSD SDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAVSQPIRTDLPPPPPPP PVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERRKREEHQRWYEKEKARLEEERERKRREQERKL GQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSL SPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDE EEEDDDVDTMLIMQRLEAERRARDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQE EGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPD SLFTAKFVAYNEEEEEEDCSLAGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKE RQRLFSQGQDVSNKVKASRKLTELENELNTK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001291964
ORF Size	5343 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291964.1, NP_001278893.1
RefSeq Size	7750 bp
RefSeq ORF	5346 bp
Locus ID	4301
UniProt ID	P55196
Protein Families	Druggable Genome
Protein Pathways	Adherens junction, Leukocyte transendothelial migration, Tight junction
MW	202.3 kDa
Gene Summary	This gene encodes a multi-domain protein involved in signaling and organization of cell junctions during embryogenesis. It has also been identified as the fusion partner of acute lymphoblastic leukemia (ALL-1) gene, involved in acute myeloid leukemias with t(6;11)(q27;q23) translocation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, however, not all have been fully characterized.[provided by RefSeq, May 2011]

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