MAST4 (NM_001290227) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC240246 representing NM_001290227 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATATGTCTGACCCCAATTTTTGGACTGTGCTCTCAAACTTTACTTTGCCTCATTTGAGGAGTGGGA ACAGGCTTCGGCGAACACAAAGTTGCCGAACAAGCAACCGGAAAAGCTTAATAGGCAATGGGCAGTCACC AGCATTGCCTCGACCACACTCACCTCTCTCTGCTCATGCAGGAAATAGCCCTCAAGATAGTCCAAGAAAT TTCTCCCCCAGTGCCTCAGCCCATTTTTCATTTGCACGGAGGACTGATGGACGCCGCTGGTCGTTGGCTT CTCTCCCTTCCTCTGGCTATGGGACAAACACACCCAGCTCTACGGTCTCTTCATCCTGTTCCTCCCAGGA GAAGTTGCATCAGTTACCATACCAACCAACACCAGACGAGTTACACTTCTTATCAAAACATTTCTGTACC ACCGAAAGCATCGCCACTGAGAACAGATGCAGGAACACGCCGATGCGCCCCCGTTCCCGAAGTCTGAGCC CTGGACGTTCTCCCGCCTGCTGTGACCATGAAATAATTATGATGAACCATGTCTACAAAGAAAGGTTCCC AAAGGCTACAGCTCAGATGGAAGAACGTCTAAAGGAAATTATCACCAGCTACTCTCCTGACAACGTTCTA CCCTTAGCAGATGGAGTGCTTAGTTTCACTCACCACCAGATTATTGAACTGGCTCGAGATTGCTTGGATA AATCCCACCAGGGCCTCATCACCTCACGATACTTCCTTGAATTACAGCACAAATTAGATAAGTTGCTACA GGAGGCTCATGATCGTTCAGAAAGTGGAGAATTGGCATTTATTAAACAACTAGTTCGAAAGATCCTAATT GTTATTGCCCGCCCTGCTCGGTTATTAGAGTGCCTGGAATTTGATCCGGAAGAATTTTACTACCTATTGG AAGCAGCAGAAGGCCATGCCAAAGAAGGACAGGGTATTAAAACCGACATTCCCAGGTACATCATTAGCCA ACTGGGACTCAATAAGGATCCCTTGGAAGAAATGGCTCATTTGGGAAACTACGATAGTGGGACAGCAGAA ACACCAGAAACAGATGAATCAGTGAGTAGCTCTAATGCCTCCCTGAAACTTCGAAGGAAACCTCGGGAAA GTGATTTTGAAACGATTAAATTGATTAGCAATGGAGCCTATGGGGCAGTCTACTTTGTTCGGCATAAAGA ATCCCGGCAGAGGTTTGCCATGAAGAAGATTAATAAACAGAACCTCATCCTTCGAAACCAGATCCAGCAG GCCTTTGTGGAGCGGGATATCCTGACTTTTGCAGAAAACCCCTTTGTTGTCAGCATGTATTGCTCCTTTG AAACAAGGCGCCACTTGTGCATGGTCATGGAATATGTGGAAGGGGGAGACTGTGCTACTTTAATGAAAAA CATGGGTCCTCTCCCTGTTGATATGGCCAGAATGTACTTTGCTGAGACGGTCTTGGCCTTGGAATATTTA CATAATTATGGAATTGTACACAGGGATTTGAAACCAGACAACTTGTTGGTTACCTCCATGGGGCACATAA AGCTGACAGATTTTGGATTATCTAAGGTGGGACTAATGAGCATGACTACCAACCTTTACGAGGGTCATAT TGAGAAGGATGCTAGAGAGTTCCTGGATAAACAGGTCTGTGGCACACCTGAATACATTGCACCAGAAGTG ATTCTGAGGCAGGGTTATGGAAAGCCGGTGGACTGGTGGGCCATGGGGATTATCCTCTATGAATTTCTGG TTGGATGCGTGCCATTCTTTGGGGATACTCCAGAGGAGCTATTTGGACAAGTCATCAGTGATGAGATCAA CTGGCCTGAGAAGGATGAGGCACCCCCACCTGATGCCCAGGATCTGATTACCTTACTCCTCAGGCAGAAT CCCCTGGAGAGGCTGGGAACAGGTGGTGCATATGAAGTCAAACAGCATCGATTCTTCCGTTCTTTAGACT GGAACAGTTTGCTGAGACAGAAGGCAGAATTTATTCCCCAACTGGAATCTGAGGATGACACAAGTTATTT TGATACTCGGTCTGAGAAGTATCATCATATGGAAACGGAGGAAGAAGATGACACAAATGATGAAGACTTT AATGTGGAAATAAGGCAGTTTTCTTCATGTTCACACAGGTTTTCAAAAGTTTTCAGCAGTATAGATCGAA TCACTCAGAATTCAGCAGAAGAGAAGGAAGACTCTGTGGACAAAACCAAAAGCACCACCTTGCCATCCAC AGAAACACTGAGCTGGAGTTCAGAATATTCTGAAATGCAACAGCTATCAACATCCAACTCTTCAGATACT GAAAGCAACAGACATAAACTCAGTTCTGGCCTACTTCCCAAACTGGCTATTTCAACAGAGGGAGAGCAAG ATGAAGCTGCCTCCTGCCCTGGAGACCCCCATGAGGAGCCAGGAAAGCCAGCCCTTCCTCCTGAAGAGTG TGCCCAGGAGGAGCCTGAGGTCACCACCCCAGCCAGCACCATCAGCAGCTCCACCCTGTCAGATATGTTT GCTGTTTCCCCTCTGGGAAGTCCAATGTCTCCCCATTCCCTGTCCTCGGACCCTTCTTCTTCACGAGATT CCTCTCCCAGCCGAGATTCCTCAGCAGCTTCTGCCAGTCCACATCAGCCGATTGTGATCCACAGTTCGGG GAAGAACTACGGCTTTACCATCCGAGCCATCCGGGTGTATGTGGGAGACAGTGACATCTATACAGTGCAC CATATCGTCTGGAATGTAGAAGAAGGAAGTCCGGCATGCCAGGCAGGACTGAAGGCTGGAGATCTTATCA CTCACATCAATGGAGAACCAGTGCATGGACTTGTCCACACAGAAGTTATAGAACTCCTACTGAAGAGTGG GAATAAGGTGTCAATCACTACTACCCCATTTGAAAACACATCAATCAAAACTGGACCAGCCAGGAGAAAC AGCTATAAGAGCCGGATGGTGAGGCGGAGCAAGAAATCCAAGAAGAAAGAAAGTCTCGAAAGGAGGAGAT CTCTTTTCAAAAAGCTAGCCAAGCAGCCTTCTCCTTTACTCCACACCAGCCGAAGTTTCTCCTGCTTGAA CAGATCCCTGTCATCGGGTGAGAGCCTCCCAGGTTCCCCCACTCATAGCTTGTCTCCCCGGTCTCCAACA CCAAGCTACCGCTCCACCCCTGACTTCCCATCTGGTACTAATTCCTCCCAGAGCAGCTCCCCTAGTTCTA GTGCCCCCAATTCCCCAGCAGGGTCCGGGCACATCCGGCCCAGCACTCTCCACGGTCTTGCACCCAAACT CGGCGGGCAGCGGTACCGGTCCGGAAGGCGAAAGTCCGCCGGCAACATCCCACTGTCCCCGCTGGCCCGG ACGCCCTCTCCAACCCCGCAACCCACCTCCCCGCAGCGGTCACCATCCCCTCTTCTGGGACACTCACTGG GCAATTCCAAGATCGCGCAAGCCTTTCCCAGCAAGATGCACTCCCCGCCCACCATCGTCAGACACATCGT GAGGCCCAAGAGTGCGGAGCCCCCCAGGTCCCCGCTGCTCAAGCGCGTGCAGTCCGAGGAGAAGCTGTCG CCCTCTTACGGCAGTGACAAGAAGCACCTGTGCTCCCGCAAGCACAGCCTGGAGGTGACCCAAGAGGAGG TGCAGCGGGAGCAGTCCCAGCGGGAGGCGCCGCTGCAGAGCCTGGATGAGAACGTGTGCGACGTGCCGCC GCTCAGCCGCGCCCGGCCAGTGGAGCAAGGCTGCCTGAAACGCCCAGTCTCCCGGAAGGTGGGCCGCCAG GAGTCTGTGGACGACCTGGACCGCGACAAGCTGAAGGCCAAGGTGGTGGTGAAGAAAGCAGACGGCTTCC CAGAGAAACAGGAATCCCACCAGAAATCCCATGGACCCGGGAGTGATTTGGAAAACTTTGCTCTGTTTAA GCTGGAAGAGAGAGAGAAGAAAGTCTATCCGAAGGCTGTGGAAAGGTCAAGTACTTTTGAAAACAAAGCG TCTATGCAGGAGGCGCCACCGCTGGGCAGCCTGCTGAAGGATGCTCTTCACAAGCAGGCCAGCGTGCGCG CCAGCGAGGGTGCGATGTCGGATGGCCGGGTGCCTGCGGAGCACCGCCAGGGTGGCGGGGACTTCAGACG GGCCCCCGCTCCTGGCACCCTCCAGGATGGTCTCTGCCACTCCCTCGACAGGGGCATCTCTGGGAAGGGG GAAGGCACGGAGAAGTCCTCCCAGGCCAAGGAGCTTCTCCGATGTGAAAAGTTAGACAGCAAGCTGGCCA ACATCGATTACCTCCGAAAGAAAATGTCACTTGAGGACAAAGAGGACAACCTCTGCCCTGTGCTGAAGCC CAAGATGACAGCTGGCTCCCACGAATGCCTGCCAGGGAACCCAGTCCGACCCACGGGTGGGCAGCAGGAG CCCCCGCCGGCTTCTGAGAGCCGAGCTTTTGTCAGCAGCACCCATGCAGCTCAGATGAGTGCCGTCTCTT TTGTTCCCCTCAAGGCCTTAACAGGCCGGGTGGACAGTGGAACGGAGAAGCCTGGCTTGGTTGCTCCTGA GTCCCCTGTTAGGAAGAGCCCCTCCGAGTATAAGCTGGAAGGTAGGTCTGTCTCATGCCTGAAGCCGATC GAGGGCACTCTGGACATTGCTCTCCTGTCCGGACCTCAGGCCTCCAAGACAGAACTGCCTTCCCCAGAGT CTGCACAGAGCCCCAGCCCAAGTGGTGACGTGAGGGCCTCTGTGCCACCAGTTCTCCCCAGCAGCAGTGG GAAAAAGAACGATACCACCAGTGCAAGAGAGCTTTCTCCTTCCAGCTTAAAGATGAATAAATCCTACCTG CTGGAGCCTTGGTTCCTGCCCCCCAGCCGAGGTCTCCAGAATTCACCAGCAGTTTCCCTGCCTGACCCAG AGTTCAAGAGGGACAGGAAAGGTCCCCATCCTACTGCCAGGAGCCCTGGAACAGTCATGGAAAGCAATCC CCAACAGAGAGAGGGCAGCTCCCCTAAACACCAAGACCACACCACTGACCCCAAGCTTCTGACCTGCCTG GGGCAGAACCTCCACAGCCCTGACCTGGCCAGGCCACGCTGCCCGCTCCCACCTGAAGCTTCCCCCTCAA GGGAGAAGCCAGGCCTGAGGGAATCGTCTGAAAGAGGCCCTCCCACAGCCAGAAGCGAGCGCTCTGCTGC GAGGGCTGACACATGCAGAGAGCCCTCCATGGAACTGTGCTTTCCAGAAACTGCGAAAACCAGTGACAAC TCCAAAAATCTCCTCTCTGTGGGAAGGACCCACCCAGATTTCTATACACAGACCCAGGCCATGGAGAAAG CATGGGCGCCGGGTGGGAAAACGAACCACAAAGATGGCCCAGGTGAGGCGAGGCCCCCGCCCAGAGACAA CTCCTCTCTGCACTCAGCTGGAATTCCCTGTGAGAAGGAGCTGGGCAAGGTGAGGCGTGGCGTGGAACCC AAGCCCGAAGCGCTTCTTGCCAGGCGGTCTCTGCAGCCACCTGGAATTGAGAGTGAGAAGAGTGAAAAGC TCTCCAGTTTCCCATCTTTGCAGAAAGATGGTGCCAAGGAACCTGAAAGGAAGGAGCAGCCTCTACAAAG GCATCCCAGCAGCATCCCTCCGCCCCCTCTGACGGCCAAAGACCTGTCCAGCCCGGCTGCCAGGCAGCAT TGCAGTTCCCCAAGCCACGCTTCTGGCAGAGAGCCGGGGGCCAAGCCCAGCACTGCAGAGCCCAGCTCGA GCCCCCAGGACCCTCCCAAGCCTGTTGCTGCGCACAGTGAAAGCAGCAGCCACAAGCCCCGGCCTGGCCC TGACCCGGGCCCTCCAAAGACTAAGCACCCCGACCGGTCCCTCTCCTCTCAGAAACCAAGTGTCGGGGCC ACAAAGGGCAAAGAGCCTGCCACTCAGTCCCTCGGTGGCTCTAGCAGAGAGGGGAAGGGCCACAGTAAGA GTGGGCCGGATGTGTTTCCTGCTACCCCAGGCTCCCAGAACAAAGCCAGCGATGGGATTGGCCAGGGAGA AGGTGGGCCCTCTGTCCCACTGCACACTGACAGGGCTCCTCTAGACGCCAAGCCACAACCCACCAGTGGT GGGCGGCCCCTGGAGGTGCTGGAGAAGCCTGTGCATTTGCCAAGGCCGGGACACCCAGGGCCTAGTGAGC CAGCGGACCAGAAACTGTCCGCTGTTGGTGAAAAGCAAACCCTGTCTCCAAAGCACCCCAAACCATCCAC TGTGAAAGATTGCCCCACCCTGTGCAAACAGACAGACAACAGACAGACAGACAAAAGCCCGAGTCAGCCG GCCGCCAACACCGACAGAAGGGCGGAAGGGAAGAAATGCACTGAAGCACTTTATGCTCCAGCAGAGGGCG ACAAGCTCGAGGCCGGCCTTTCCTTTGTGCATAGCGAGAACCGGTTGAAAGGCGCGGAGCGGCCAGCCGC GGGGGTGGGGAAGGGCTTCCCTGAGGCCAGAGGGAAAGGGCCCGGTCCCCAGAAGCCACCGACGGAGGCA GACAAGCCCAATGGCATGAAACGGTCCCCCTCAGCCACTGGGCAGAGTTCTTTCCGATCCACGGCCCTCC CGGAAAAGTCTCTGAGCTGCTCCTCCAGCTTCCCTGAAACCAGGGCCGGAGTTAGAGAGGCCTCTGCAGC CAGCAGCGACACCTCTTCTGCCAAGGCCGCCGGGGGCATGCTGGAGCTTCCAGCCCCCAGCAACAGGGAC CATAGGAAGGCTCAGCCTGCCGGGGAGGGCCGAACCCACATGACAAAGAGTGACTCCCTGCCCTCCTTCC GGGTCTCCACCCTGCCTCTGGAGTCACACCACCCCGACCCAAACACCATGGGCGGGGCCAGCCACCGGGA CAGGGCTCTCTCGGTGACTGCCACCGTAGGGGAAACCAAAGGGAAGGACCCTGCCCCAGCCCAGCCTCCC CCAGCTAGGAAACAGAACGTGGGCAGAGACGTGACCAAGCCATCCCCAGCCCCAAACACTGACCGCCCCA TCTCTCTTTCTAATGAGAAGGACTTTGTGGTACGGCAGAGGCGGGGGAAAGAGAGTTTGCGTAGCAGCCC TCACAAAAAGGCCTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC240246 representing NM_001290227 Red=Cloning site Green=Tags(s) MDMSDPNFWTVLSNFTLPHLRSGNRLRRTQSCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRN FSPSASAHFSFARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCT TESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDNVL PLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFLELQHKLDKLLQEAHDRSESGELAFIKQLVRKILI VIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAHLGNYDSGTAE TPETDESVSSSNASLKLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQ AFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYL HNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEV ILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQDLITLLLRQN PLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDF NVEIRQFSSCSHRFSKVFSSIDRITQNSAEEKEDSVDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDT ESNRHKLSSGLLPKLAISTEGEQDEAASCPGDPHEEPGKPALPPEECAQEEPEVTTPASTISSSTLSDMF AVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVH HIVWNVEEGSPACQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRN SYKSRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPT PSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLGGQRYRSGRRKSAGNIPLSPLAR TPSPTPQPTSPQRSPSPLLGHSLGNSKIAQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLS PSYGSDKKHLCSRKHSLEVTQEEVQREQSQREAPLQSLDENVCDVPPLSRARPVEQGCLKRPVSRKVGRQ ESVDDLDRDKLKAKVVVKKADGFPEKQESHQKSHGPGSDLENFALFKLEEREKKVYPKAVERSSTFENKA SMQEAPPLGSLLKDALHKQASVRASEGAMSDGRVPAEHRQGGGDFRRAPAPGTLQDGLCHSLDRGISGKG EGTEKSSQAKELLRCEKLDSKLANIDYLRKKMSLEDKEDNLCPVLKPKMTAGSHECLPGNPVRPTGGQQE PPPASESRAFVSSTHAAQMSAVSFVPLKALTGRVDSGTEKPGLVAPESPVRKSPSEYKLEGRSVSCLKPI EGTLDIALLSGPQASKTELPSPESAQSPSPSGDVRASVPPVLPSSSGKKNDTTSARELSPSSLKMNKSYL LEPWFLPPSRGLQNSPAVSLPDPEFKRDRKGPHPTARSPGTVMESNPQQREGSSPKHQDHTTDPKLLTCL GQNLHSPDLARPRCPLPPEASPSREKPGLRESSERGPPTARSERSAARADTCREPSMELCFPETAKTSDN SKNLLSVGRTHPDFYTQTQAMEKAWAPGGKTNHKDGPGEARPPPRDNSSLHSAGIPCEKELGKVRRGVEP KPEALLARRSLQPPGIESEKSEKLSSFPSLQKDGAKEPERKEQPLQRHPSSIPPPPLTAKDLSSPAARQH CSSPSHASGREPGAKPSTAEPSSSPQDPPKPVAAHSESSSHKPRPGPDPGPPKTKHPDRSLSSQKPSVGA TKGKEPATQSLGGSSREGKGHSKSGPDVFPATPGSQNKASDGIGQGEGGPSVPLHTDRAPLDAKPQPTSG GRPLEVLEKPVHLPRPGHPGPSEPADQKLSAVGEKQTLSPKHPKPSTVKDCPTLCKQTDNRQTDKSPSQP AANTDRRAEGKKCTEALYAPAEGDKLEAGLSFVHSENRLKGAERPAAGVGKGFPEARGKGPGPQKPPTEA DKPNGMKRSPSATGQSSFRSTALPEKSLSCSSSFPETRAGVREASAASSDTSSAKAAGGMLELPAPSNRD HRKAQPAGEGRTHMTKSDSLPSFRVSTLPLESHHPDPNTMGGASHRDRALSVTATVGETKGKDPAPAQPP PARKQNVGRDVTKPSPAPNTDRPISLSNEKDFVVRQRRGKESLRSSPHKKAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001290227
ORF Size	7086 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290227.2
RefSeq Size	9747 bp
RefSeq ORF	7089 bp
Locus ID	375449
Protein Families	Druggable Genome, Protein Kinase
MW	258.1 kDa
Gene Summary	This gene encodes a member of the microtubule-associated serine/threonine protein kinases. The proteins in this family contain a domain that gives the kinase the ability to determine its own scaffold to control the effects of their kinase activities. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2014]

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