MAST4 (NM_001297651) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC240249 representing NM_001297651 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATATGTCTGACCCCAATTTTTGGACTGTGCTCTCAAACTTTACTTTGCCTCATTTGAGGAGTGGGA ACAGGCTTCGGCGAACACAAAGTTGCCGAACAAGCAACCGGAAAAGCTTAATAGGCAATGGGCAGTCACC AGCATTGCCTCGACCACACTCACCTCTCTCTGCTCATGCAGGAAATAGCCCTCAAGATAGTCCAAGAAAT TTCTCCCCCAGTGCCTCAGCCCATTTTTCATTTGCACGGAGGACTGATGGACGCCGCTGGTCGTTGGCTT CTCTCCCTTCCTCTGGCTATGGGACAAACACACCCAGCTCTACGGTCTCTTCATCCTGTTCCTCCCAGGA GAAGTTGCATCAGTTACCATACCAACCAACACCAGACGAGTTACACTTCTTATCAAAACATTTCTGTACC ACCGAAAGCATCGCCACTGAGAACAGATGCAGGAACACGCCGATGCGCCCCCGTTCCCGAAGTCTGAGCC CTGGACGTTCTCCCGCCTGCTGTGACCATGAAATAATTATGATGAACCATGTCTACAAAGAAAGGTTCCC AAAGGCTACAGCTCAGATGGAAGAACGTCTAAAGGAAATTATCACCAGCTACTCTCCTGACAACGTTCTA CCCTTAGCAGATGGAGTGCTTAGTTTCACTCACCACCAGATTATTGAACTGGCTCGAGATTGCTTGGATA AATCCCACCAGGGCCTCATCACCTCACGATACTTCCTTGAATTACAGCACAAATTAGATAAGTTGCTACA GGAGGCTCATGATCGTTCAGAAAGTGGAGAATTGGCATTTATTAAACAACTAGTTCGAAAGATCCTAATT GTTATTGCCCGCCCTGCTCGGTTATTAGAGTGCCTGGAATTTGATCCGGAAGAATTTTACTACCTATTGG AAGCAGCAGAAGGCCATGCCAAAGAAGGACAGGGTATTAAAACCGACATTCCCAGGTACATCATTAGCCA ACTGGGACTCAATAAGGATCCCTTGGAAGAAATGGCTCATTTGGGAAACTACGATAGTGGGACAGCAGAA ACACCAGAAACAGATGAATCAGTGAGTAGCTCTAATGCCTCCCTGAAACTTCGAAGGAAACCTCGGGAAA GTGATTTTGAAACGATTAAATTGATTAGCAATGGAGCCTATGGGGCAGTCTACTTTGTTCGGCATAAAGA ATCCCGGCAGAGGTTTGCCATGAAGAAGATTAATAAACAGAACCTCATCCTTCGAAACCAGATCCAGCAG GCCTTTGTGGAGCGGGATATCCTGACTTTTGCAGAAAACCCCTTTGTTGTCAGCATGTATTGCTCCTTTG AAACAAGGCGCCACTTGTGCATGGTCATGGAATATGTGGAAGGGGGAGACTGTGCTACTTTAATGAAAAA CATGGGTCCTCTCCCTGTTGATATGGCCAGAATGTACTTTGCTGAGACGGTCTTGGCCTTGGAATATTTA CATAATTATGGAATTGTACACAGGGATTTGAAACCAGACAACTTGTTGGTTACCTCCATGGGGCACATAA AGCTGACAGATTTTGGATTATCTAAGGTGGGACTAATGAGCATGACTACCAACCTTTACGAGGGTCATAT TGAGAAGGATGCTAGAGAGTTCCTGGATAAACAGGTCTGTGGCACACCTGAATACATTGCACCAGAAGTG ATTCTGAGGCAGGGTTATGGAAAGCCGGTGGACTGGTGGGCCATGGGGATTATCCTCTATGAATTTCTGG TTGGATGCGTGCCATTCTTTGGGGATACTCCAGAGGAGCTATTTGGACAAGTCATCAGTGATGAGATCAA CTGGCCTGAGAAGGATGAGGCACCCCCACCTGATGCCCAGGATCTGATTACCTTACTCCTCAGGCAGAAT CCCCTGGAGAGGCTGGGAACAGGTGGTGCATATGAAGTCAAACAGCATCGATTCTTCCGTTCTTTAGACT GGAACAGTTTGCTGAGACAGAAGGCAGAATTTATTCCCCAACTGGAATCTGAGGATGACACAAGTTATTT TGATACTCGGTCTGAGAAGTATCATCATATGGAAACGGAGGAAGAAGATGACACAAATGATGAAGACTTT AATGTGGAAATAAGGCAGTTTTCTTCATGTTCACACAGGTTTTCAAAAGTTTTCAGCAGTATAGATCGAA TCACTCAGAATTCAGCAGAAGAGAAGGAAGACTCTGTGGACAAAACCAAAAGCACCACCTTGCCATCCAC AGAAACACTGAGCTGGAGTTCAGAATATTCTGAAATGCAACAGCTATCAACATCCAACTCTTCAGATACT GAAAGCAACAGACATAAACTCAGTTCTGGCCTACTTCCCAAACTGGCTATTTCAACAGAGGGAGAGCAAG ATGAAGCTGCCTCCTGCCCTGGAGACCCCCATGAGGAGCCAGGAAAGCCAGCCCTTCCTCCTGAAGAGTG TGCCCAGGAGGAGCCTGAGGTCACCACCCCAGCCAGCACCATCAGCAGCTCCACCCTGTCAGTTGGCAGT TTTTCAGAGCACTTGGATCAGATAAATGGACGAAGCGAGTGTGTGGACAGTACAGATAATTCCTCAAAGC CATCCAGTGAACCCGCTTCTCACATGGCTCGGCAGCGATTAGAAAGCACAGAAAAAAAGAAAATCTCGGG GAAAGTCACAAAGTCCCTCTCTGCCAGTGCTCTTTCCCTCATGATCCCAGGAGATATGTTTGCTGTTTCC CCTCTGGGAAGTCCAATGTCTCCCCATTCCCTGTCCTCGGACCCTTCTTCTTCACGAGATTCCTCTCCCA GCCGAGATTCCTCAGCAGCTTCTGCCAGTCCACATCAGCCGATTGTGATCCACAGTTCGGGGAAGAACTA CGGCTTTACCATCCGAGCCATCCGGGTGTATGTGGGAGACAGTGACATCTATACAGTGCACCATATCGTC TGGAATGTAGAAGAAGGAAGTCCGGCATGCCAGGCAGGACTGAAGGCTGGAGATCTTATCACTCACATCA ATGGAGAACCAGTGCATGGACTTGTCCACACAGAAGTTATAGAACTCCTACTGAAGAGTGGGAATAAGGT GTCAATCACTACTACCCCATTTGAAAACACATCAATCAAAACTGGACCAGCCAGGAGAAACAGCTATAAG AGCCGGATGGTGAGGCGGAGCAAGAAATCCAAGAAGAAAGAAAGTCTCGAAAGGAGGAGATCTCTTTTCA AAAAGCTAGCCAAGCAGCCTTCTCCTTTACTCCACACCAGCCGAAGTTTCTCCTGCTTGAACAGATCCCT GTCATCGGGTGAGAGCCTCCCAGGTTCCCCCACTCATAGCTTGTCTCCCCGGTCTCCAACACCAAGCTAC CGCTCCACCCCTGACTTCCCATCTGGTACTAATTCCTCCCAGAGCAGCTCCCCTAGTTCTAGTGCCCCCA ATTCCCCAGCAGGGTCCGGGCACATCCGGCCCAGCACTCTCCACGGTCTTGCACCCAAACTCGGCGGGCA GCGGTACCGGTCCGGAAGGCGAAAGTCCGCCGGCAACATCCCACTGTCCCCGCTGGCCCGGACGCCCTCT CCAACCCCGCAACCCACCTCCCCGCAGCGGTCACCATCCCCTCTTCTGGGACACTCACTGGGCAATTCCA AGATCGCGCAAGCCTTTCCCAGCAAGATGCACTCCCCGCCCACCATCGTCAGACACATCGTGAGGCCCAA GAGTGCGGAGCCCCCCAGGTCCCCGCTGCTCAAGCGCGTGCAGTCCGAGGAGAAGCTGTCGCCCTCTTAC GGCAGTGACAAGAAGCACCTGTGCTCCCGCAAGCACAGCCTGGAGGTGACCCAAGAGGAGGTGCAGCGGG AGCAGTCCCAGCGGGAGGCGCCGCTGCAGAGCCTGGATGAGAACGTGTGCGACGTGCCGCCGCTCAGCCG CGCCCGGCCAGTGGAGCAAGGCTGCCTGAAACGCCCAGTCTCCCGGAAGGTGGGCCGCCAGGAGTCTGTG GACGACCTGGACCGCGACAAGCTGAAGGCCAAGGTGGTGGTGAAGAAAGCAGACGGCTTCCCAGAGAAAC AGGAATCCCACCAGAAATCCCATGGACCCGGGAGTGATTTGGAAAACTTTGCTCTGTTTAAGCTGGAAGA GAGAGAGAAGAAAGTCTATCCGAAGGCTGTGGAAAGGTCAAGTACTTTTGAAAACAAAGCGTCTATGCAG GAGGCGCCACCGCTGGGCAGCCTGCTGAAGGATGCTCTTCACAAGCAGGCCAGCGTGCGCGCCAGCGAGG GTGCGATGTCGGATGGCCGGGTGCCTGCGGAGCACCGCCAGGGTGGCGGGGACTTCAGACGGGCCCCCGC TCCTGGCACCCTCCAGGATGGTCTCTGCCACTCCCTCGACAGGGGCATCTCTGGGAAGGGGGAAGGCACG GAGAAGTCCTCCCAGGCCAAGGAGCTTCTCCGATGTGAAAAGTTAGACAGCAAGCTGGCCAACATCGATT ACCTCCGAAAGAAAATGTCACTTGAGGACAAAGAGGACAACCTCTGCCCTGTGCTGAAGCCCAAGATGAC AGCTGGCTCCCACGAATGCCTGCCAGGGAACCCAGTCCGACCCACGGGTGGGCAGCAGGAGCCCCCGCCG GCTTCTGAGAGCCGAGCTTTTGTCAGCAGCACCCATGCAGCTCAGATGAGTGCCGTCTCTTTTGTTCCCC TCAAGGCCTTAACAGGCCGGGTGGACAGTGGAACGGAGAAGCCTGGCTTGGTTGCTCCTGAGTCCCCTGT TAGGAAGAGCCCCTCCGAGTATAAGCTGGAAGGTAGGTCTGTCTCATGCCTGAAGCCGATCGAGGGCACT CTGGACATTGCTCTCCTGTCCGGACCTCAGGCCTCCAAGACAGAACTGCCTTCCCCAGAGTCTGCACAGA GCCCCAGCCCAAGTGGTGACGTGAGGGCCTCTGTGCCACCAGTTCTCCCCAGCAGCAGTGGGAAAAAGAA CGATACCACCAGTGCAAGAGAGCTTTCTCCTTCCAGCTTAAAGATGAATAAATCCTACCTGCTGGAGCCT TGGTTCCTGCCCCCCAGCCGAGGTCTCCAGAATTCACCAGCAGTTTCCCTGCCTGACCCAGAGTTCAAGA GGGACAGGAAAGGTCCCCATCCTACTGCCAGGAGCCCTGGAACAGTCATGGAAAGCAATCCCCAACAGAG AGAGGGCAGCTCCCCTAAACACCAAGACCACACCACTGACCCCAAGCTTCTGACCTGCCTGGGGCAGAAC CTCCACAGCCCTGACCTGGCCAGGCCACGCTGCCCGCTCCCACCTGAAGCTTCCCCCTCAAGGGAGAAGC CAGGCCTGAGGGAATCGTCTGAAAGAGGCCCTCCCACAGCCAGAAGCGAGCGCTCTGCTGCGAGGGCTGA CACATGCAGAGAGCCCTCCATGGAACTGTGCTTTCCAGAAACTGCGAAAACCAGTGACAACTCCAAAAAT CTCCTCTCTGTGGGAAGGACCCACCCAGATTTCTATACACAGACCCAGGCCATGGAGAAAGCATGGGCGC CGGGTGGGAAAACGAACCACAAAGATGGCCCAGGTGAGGCGAGGCCCCCGCCCAGAGACAACTCCTCTCT GCACTCAGCTGGAATTCCCTGTGAGAAGGAGCTGGGCAAGGTGAGGCGTGGCGTGGAACCCAAGCCCGAA GCGCTTCTTGCCAGGCGGTCTCTGCAGCCACCTGGAATTGAGAGTGAGAAGAGTGAAAAGCTCTCCAGTT TCCCATCTTTGCAGAAAGATGGTGCCAAGGAACCTGAAAGGAAGGAGCAGCCTCTACAAAGGCATCCCAG CAGCATCCCTCCGCCCCCTCTGACGGCCAAAGACCTGTCCAGCCCGGCTGCCAGGCAGCATTGCAGTTCC CCAAGCCACGCTTCTGGCAGAGAGCCGGGGGCCAAGCCCAGCACTGCAGAGCCCAGCTCGAGCCCCCAGG ACCCTCCCAAGCCTGTTGCTGCGCACAGTGAAAGCAGCAGCCACAAGCCCCGGCCTGGCCCTGACCCGGG CCCTCCAAAGACTAAGCACCCCGACCGGTCCCTCTCCTCTCAGAAACCAAGTGTCGGGGCCACAAAGGGC AAAGAGCCTGCCACTCAGTCCCTCGGTGGCTCTAGCAGAGAGGGGAAGGGCCACAGTAAGAGTGGGCCGG ATGTGTTTCCTGCTACCCCAGGCTCCCAGAACAAAGCCAGCGATGGGATTGGCCAGGGAGAAGGTGGGCC CTCTGTCCCACTGCACACTGACAGGGCTCCTCTAGACGCCAAGCCACAACCCACCAGTGGTGGGCGGCCC CTGGAGGTGCTGGAGAAGCCTGTGCATTTGCCAAGGCCGGGACACCCAGGGCCTAGTGAGCCAGCGGACC AGAAACTGTCCGCTGTTGGTGAAAAGCAAACCCTGTCTCCAAAGCACCCCAAACCATCCACTGTGAAAGA TTGCCCCACCCTGTGCAAACAGACAGACAACAGACAGACAGACAAAAGCCCGAGTCAGCCGGCCGCCAAC ACCGACAGAAGGGCGGAAGGGAAGAAATGCACTGAAGCACTTTATGCTCCAGCAGAGGGCGACAAGCTCG AGGCCGGCCTTTCCTTTGTGCATAGCGAGAACCGGTTGAAAGGCGCGGAGCGGCCAGCCGCGGGGGTGGG GAAGGGCTTCCCTGAGGCCAGAGGGAAAGGGCCCGGTCCCCAGAAGCCACCGACGGAGGCAGACAAGCCC AATGGCATGAAACGGTCCCCCTCAGCCACTGGGCAGAGTTCTTTCCGATCCACGGCCCTCCCGGAAAAGT CTCTGAGCTGCTCCTCCAGCTTCCCTGAAACCAGGGCCGGAGTTAGAGAGGCCTCTGCAGCCAGCAGCGA CACCTCTTCTGCCAAGGCCGCCGGGGGCATGCTGGAGCTTCCAGCCCCCAGCAACAGGGACCATAGGAAG GCTCAGCCTGCCGGGGAGGGCCGAACCCACATGACAAAGAGTGACTCCCTGCCCTCCTTCCGGGTCTCCA CCCTGCCTCTGGAGTCACACCACCCCGACCCAAACACCATGGGCGGGGCCAGCCACCGGGACAGGGCTCT CTCGGTGACTGCCACCGTAGGGGAAACCAAAGGGAAGGACCCTGCCCCAGCCCAGCCTCCCCCAGCTAGG AAACAGAACGTGGGCAGAGACGTGACCAAGCCATCCCCAGCCCCAAACACTGACCGCCCCATCTCTCTTT CTAATGAGAAGGACTTTGTGGTACGGCAGAGGCGGGGGAAAGAGAGTTTGCGTAGCAGCCCTCACAAAAA GGCCTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC240249 representing NM_001297651 Red=Cloning site Green=Tags(s) MDMSDPNFWTVLSNFTLPHLRSGNRLRRTQSCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRN FSPSASAHFSFARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCT TESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDNVL PLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFLELQHKLDKLLQEAHDRSESGELAFIKQLVRKILI VIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAHLGNYDSGTAE TPETDESVSSSNASLKLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQ AFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYL HNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEV ILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQDLITLLLRQN PLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDF NVEIRQFSSCSHRFSKVFSSIDRITQNSAEEKEDSVDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDT ESNRHKLSSGLLPKLAISTEGEQDEAASCPGDPHEEPGKPALPPEECAQEEPEVTTPASTISSSTLSVGS FSEHLDQINGRSECVDSTDNSSKPSSEPASHMARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVS PLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIV WNVEEGSPACQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYK SRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSY RSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLGGQRYRSGRRKSAGNIPLSPLARTPS PTPQPTSPQRSPSPLLGHSLGNSKIAQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSY GSDKKHLCSRKHSLEVTQEEVQREQSQREAPLQSLDENVCDVPPLSRARPVEQGCLKRPVSRKVGRQESV DDLDRDKLKAKVVVKKADGFPEKQESHQKSHGPGSDLENFALFKLEEREKKVYPKAVERSSTFENKASMQ EAPPLGSLLKDALHKQASVRASEGAMSDGRVPAEHRQGGGDFRRAPAPGTLQDGLCHSLDRGISGKGEGT EKSSQAKELLRCEKLDSKLANIDYLRKKMSLEDKEDNLCPVLKPKMTAGSHECLPGNPVRPTGGQQEPPP ASESRAFVSSTHAAQMSAVSFVPLKALTGRVDSGTEKPGLVAPESPVRKSPSEYKLEGRSVSCLKPIEGT LDIALLSGPQASKTELPSPESAQSPSPSGDVRASVPPVLPSSSGKKNDTTSARELSPSSLKMNKSYLLEP WFLPPSRGLQNSPAVSLPDPEFKRDRKGPHPTARSPGTVMESNPQQREGSSPKHQDHTTDPKLLTCLGQN LHSPDLARPRCPLPPEASPSREKPGLRESSERGPPTARSERSAARADTCREPSMELCFPETAKTSDNSKN LLSVGRTHPDFYTQTQAMEKAWAPGGKTNHKDGPGEARPPPRDNSSLHSAGIPCEKELGKVRRGVEPKPE ALLARRSLQPPGIESEKSEKLSSFPSLQKDGAKEPERKEQPLQRHPSSIPPPPLTAKDLSSPAARQHCSS PSHASGREPGAKPSTAEPSSSPQDPPKPVAAHSESSSHKPRPGPDPGPPKTKHPDRSLSSQKPSVGATKG KEPATQSLGGSSREGKGHSKSGPDVFPATPGSQNKASDGIGQGEGGPSVPLHTDRAPLDAKPQPTSGGRP LEVLEKPVHLPRPGHPGPSEPADQKLSAVGEKQTLSPKHPKPSTVKDCPTLCKQTDNRQTDKSPSQPAAN TDRRAEGKKCTEALYAPAEGDKLEAGLSFVHSENRLKGAERPAAGVGKGFPEARGKGPGPQKPPTEADKP NGMKRSPSATGQSSFRSTALPEKSLSCSSSFPETRAGVREASAASSDTSSAKAAGGMLELPAPSNRDHRK AQPAGEGRTHMTKSDSLPSFRVSTLPLESHHPDPNTMGGASHRDRALSVTATVGETKGKDPAPAQPPPAR KQNVGRDVTKPSPAPNTDRPISLSNEKDFVVRQRRGKESLRSSPHKKAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001297651
ORF Size	7287 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001297651.2
RefSeq Size	10219 bp
RefSeq ORF	7290 bp
Locus ID	375449
UniProt ID	O15021
Protein Families	Druggable Genome, Protein Kinase
MW	265.2 kDa
Gene Summary	This gene encodes a member of the microtubule-associated serine/threonine protein kinases. The proteins in this family contain a domain that gives the kinase the ability to determine its own scaffold to control the effects of their kinase activities. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2014]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.