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Eph receptor B6 (EPHB6) (NM_004445) Human Tagged ORF Clone
CAT＃: RC600052
- TrueORF®
EPHB6 (DDK-His-tagged)-Extra Cellular Domain Clone of Homo sapiens EPH receptor B6, Signal peptide (1-31) plus EC domain (32-594)
"NM_004445" in other vectors (6)
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CNY 4,424.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | DDK-His |
| Synonyms | HEP |
| Vector | pCMV6-XL5-DDK-His |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>RC600052 representing leader sequence plus the extracellular domain region of NM_004445
Red=Cloning site Blue=ORF Green=Tags(s) GTAATACGACTCACTATAGGGCGGCCGCGAATTCGTCGACTGGATCTGGTACCGAGGAGATCCGCCGCCG CGATCGCC ATGGCTACTGAAGGGGCTGCCCAGTTAGGGAACAGAGTGGCGGGCATGGTGTGTAGCCTATGGGTGCTGC TCCTGGTGTCTTCAGTTCTGGCTCTGGAAGAGGTATTGCTGGACACCACCGGAGAGACATCTGAGATTGG CTGGCTCACCTACCCACCAGGGGGGTGGGACGAGGTGAGTGTTCTGGACGACCAGCGACGCCTGACTCGG ACCTTTGAGGCATGTCATGTGGCAGGGGCCCCTCCAGGCACCGGGCAGGACAATTGGTTGCAGACACACT TTGTGGAGCGGCGCGGGGCCCAGAGGGCGCACATTCGACTCCACTTCTCTGTGCGGGCATGCTCCAGCCT GGGTGTGAGCGGCGGCACCTGCCGGGAGACCTTCACCCTTTACTACCGTCAGGCTGAGGAGCCCGACAGC CCTGACAGCGTTTCCTCCTGGCACCTCAAACGCTGGACCAAGGTGGACACAATTGCAGCAGACGAGAGCT TTCCCTCCTCCTCCTCCTCCTCCTCCTCTTCTTCCTCTGCAGCGTGGGCTGTGGGACCCCACGGGGCTGG GCAGCGGGCTGGACTGCAACTGAACGTCAAAGAGCGGAGCTTTGGGCCTCTCACCCAACGCGGCTTCTAC GTGGCCTTCCAGGACACGGGGGCCTGCCTGGCCCTGGTCGCTGTCAGGCTCTTCTCCTACACCTGCCCTG CCGTGCTCCGATCCTTTGCTTCCTTTCCAGAGACGCAGGCCAGTGGGGCTGGGGGGGCCTCCCTGGTGGC AGCTGTGGGCACCTGTGTGGCTCATGCAGAGCCAGAGGAGGATGGAGTAGGGGGCCAGGCAGGAGGCAGC CCCCCCAGGCTGCACTGCAACGGGGAGGGCAAGTGGATGGTAGCTGTCGGGGGCTGCCGCTGCCAGCCTG GATACCAACCAGCACGAGGAGACAAGGCCTGCCAAGCCTGCCCACGGGGGCTCTATAAGTCTTCTGCTGG GAATGCTCCCTGCTCACCATGCCCTGCCCGCAGTCACGCTCCCAACCCAGCAGCCCCCGTTTGCCCCTGC CTGGAGGGCTTCTACCGGGCCAGTTCCGACCCACCAGAGGCCCCCTGCACTGGTCCTCCATCGGCTCCCC AGGAGCTTTGGTTTGAGGTGCAAGGCTCAGCACTCATGCTACACTGGCGCCTGCCTCGGGAGCTGGGGGG TCGAGGGGACCTGCTCTTCAATGTCGTGTGCAAGGAGTGTGAAGGCCGCCAGGAACCTGCCAGCGGTGGT GGGGGCACTTGTCACCGCTGCAGGGATGAGGTCCACTTCGACCCTCGCCAGAGAGGCCTGACTGAGAGCC GAGTGTTAGTGGGGGGACTCCGGGCACACGTACCCTACATCTTAGAGGTGCAGGCTGTTAATGGGGTGTC TGAGCTCAGCCCTGACCCTCCTCAGGCTGCAGCCATCAATGTCAGCACCAGCCATGAAGTGCCCTCTGCT GTCCCTGTGGTGCACCAGGTGAGCCGGGCATCCAACAGCATCACGGTGTCCTGGCCGCAGCCCGACCAGA CCAATGGGAACATCCTGGACTATCAGCTCCGCTACTATGACCAGGCAGAAGACGAATCCCACTCCTTCAC CCTGACCAGCGAGACCAACACTGCCACCGTGACACAGCTGAGCCCTGGCCACATCTATGGTTTCCAGGTG CGGGCCCGGACTGCTGCCGGCCACGGCCCCTACGGGGGCAAAGTCTATTTCCAGACACTTCCTCAAGGGG AGCTGTCTTCCCAGCTTCCGGAAAGACTCTCC ACGCGTTCAGGCGACTACAAGGATGACGACGATAAGGGATCTCATCATCACCATCACCATTAATGAGATC TGGTACCGATATCAAGCTTGTCGACTCTAGA >RC600052 representing signal peptide plus the extracellular domain region of NM_004445
Red=Cloning sites Green= DDK and 6XHIS Tags MATEGAAQLGNRVAGMVCSLWVLLLVSSVLALEEVLLDTTGETSEIGWLTYPPGGWDEVSVLDDQRRLTR TFEACHVAGAPPGTGQDNWLQTHFVERRGAQRAHIRLHFSVRACSSLGVSGGTCRETFTLYYRQAEEPDS PDSVSSWHLKRWTKVDTIAADESFPSSSSSSSSSSSAAWAVGPHGAGQRAGLQLNVKERSFGPLTQRGFY VAFQDTGACLALVAVRLFSYTCPAVLRSFASFPETQASGAGGASLVAAVGTCVAHAEPEEDGVGGQAGGS PPRLHCNGEGKWMVAVGGCRCQPGYQPARGDKACQACPRGLYKSSAGNAPCSPCPARSHAPNPAAPVCPC LEGFYRASSDPPEAPCTGPPSAPQELWFEVQGSALMLHWRLPRELGGRGDLLFNVVCKECEGRQEPASGG GGTCHRCRDEVHFDPRQRGLTESRVLVGGLRAHVPYILEVQAVNGVSELSPDPPQAAAINVSTSHEVPSA VPVVHQVSRASNSITVSWPQPDQTNGNILDYQLRYYDQAEDESHSFTLTSETNTATVTQLSPGHIYGFQV RARTAAGHGPYGGKVYFQTLPQGELSSQLPERLS TRSGTRSGDYKDDDDKGSHHHHHH |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NM_004445 |
| ORF Size | 1782 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the extra cellular domain of the protein with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004445.5, NP_004436.4 |
| RefSeq Size | 3449 bp |
| RefSeq ORF | 3069 bp |
| Locus ID | 2051 |
| UniProt ID | O15197 |
| Domains | pkinase, EPH_lbd, TyrKc, SAM, S_TKc, FN3 |
| Protein Families | Druggable Genome, Protein Kinase, Transmembrane |
| Protein Pathways | Axon guidance |
| MW | 63.3 kDa |
| Gene Summary | This gene encodes a member of a family of transmembrane proteins that function as receptors for ephrin-B family proteins. Unlike other members of this family, the encoded protein does not contain a functional kinase domain. Activity of this protein can influence cell adhesion and migration. Expression of this gene is downregulated during tumor progression, suggesting that the protein may suppress tumor invasion and metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC229404 | EPHB6 (Myc-DDK-tagged)-Human EPH receptor B6 (EPHB6) |
CNY 7,120.00 |
|
| RC229404L3 | Lenti-ORF clone of EPHB6 (Myc-DDK-tagged)-Human EPH receptor B6 (EPHB6) |
CNY 9,520.00 |
|
| RC229404L4 | Lenti-ORF clone of EPHB6 (mGFP-tagged)-Human EPH receptor B6 (EPHB6) |
CNY 12,070.00 |
|
| RG229404 | EPHB6 (tGFP-tagged) - Human EPH receptor B6 (EPHB6) |
CNY 11,210.00 |
|
| SC310011 | EPHB6 (untagged)-Human EPH receptor B6 (EPHB6) |
CNY 7,128.00 |
|
| SC328039 | EPHB6 (untagged)-Human EPH receptor B6 (EPHB6) |
CNY 10,672.00 |
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