RHOD (NM_014578) Human Tagged ORF Clone

CAT#: RG201722

  • TrueORF®

RHOD (tGFP-tagged) - Human ras homolog gene family, member D (RHOD)

ORF Plasmid: DDK tGFP



  "NM_014578" in other vectors (4)

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CNY 4,000.00

CNY 4,370.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


RHOD mouse monoclonal antibody, clone OTI2F7 (formerly 2F7)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms ARHD; Rho; RHOHP1; RHOM
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG201722 representing NM_014578
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACGGCGGCCCAGGCCGCGGGTGAGGAGGCGCCACCAGGCGTGCGGTCCGTCAAGGTGGTCCTGGTGG
GCGACGGCGGCTGCGGGAAGACGTCGCTGCTGATGGTCTTCGCCGATGGGGCCTTCCCCGAGAGCTACAC
CCCCACGGTGTTTGAGCGGTACATGGTCAACCTGCAAGTGAAAGGCAAACCTGTGCACCTCCACATCTGG
GACACAGCAGGGCAAGATGACTATGACCGCCTGCGGCCCCTGTTCTACCCTGACGCCAGCGTCCTGCTGC
TTTGCTTCGATGTCACCAGCCCGAACAGCTTTGACAACATCTTTAACCGGTGGTACCCAGAAGTGAATCA
TTTCTGCAAGAAGGTACCCATCATCGTCGTGGGCTGCAAGACTGACCTGCGCAAGGACAAATCACTGGTG
AACAAGCTCCGAAGAAACGGATTGGAGCCTGTGACCTACCACAGGGGCCAGGAGATGGCGAGGTCCGTGG
GCGCGGTGGCCTACCTCGAGTGCTCGGCTCGGCTCCATGACAACGTCCACGCCGTCTTCCAGGAGGCCGC
CGAGGTGGCCCTCAGCAGCCGCGGTCGCAACTTCTGGCGGCGGATTACCCAGGGCTTTTGCGTGGTGACC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG201722 representing NM_014578
Red=Cloning site Green=Tags(s)

MTAAQAAGEEAPPGVRSVKVVLVGDGGCGKTSLLMVFADGAFPESYTPTVFERYMVNLQVKGKPVHLHIW
DTAGQDDYDRLRPLFYPDASVLLLCFDVTSPNSFDNIFNRWYPEVNHFCKKVPIIVVGCKTDLRKDKSLV
NKLRRNGLEPVTYHRGQEMARSVGAVAYLECSARLHDNVHAVFQEAAEVALSSRGRNFWRRITQGFCVVT

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_014578
ORF Size 630 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_014578.4
RefSeq Size 1111 bp
RefSeq ORF 633 bp
Locus ID 29984
UniProt ID O00212
Protein Pathways Axon guidance
Gene Summary Ras homolog, or Rho, proteins interact with protein kinases and may serve as targets for activated GTPase. They play a critical role in muscle differentiation. The protein encoded by this gene binds GTP and is a member of the small GTPase superfamily. It is involved in endosome dynamics and reorganization of the actin cytoskeleton, and it may coordinate membrane transport with the function of the cytoskeleton. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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