United States
Germany
Japan
United Kingdom
China
DOK4 (NM_018110) Human Tagged ORF Clone
CAT＃: RG202561
- TrueORF®
DOK4 (tGFP-tagged) - Human docking protein 4 (DOK4)
ORF Plasmid: DDK
"NM_018110" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 4,000.00
CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | IRS-5; IRS5 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG202561 representing NM_018110
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACCAATTTCAGTGACATCGTCAAGCAAGGCTACGTGAAGATGAAGAGCAGGAAGCTCGGGATCT ACCGGAGGTGCTGGCTGGTGTTCCGGAAATCCTCCAGCAAGGGGCCCCAGCGGCTGGAGAAGTATCCAGA TGAGAAGTCGGTGTGCCTCCGGGGCTGCCCCAAGGTGACTGAGATCAGCAACGTCAAGTGTGTTACGCGG CTCCCCAAGGAGACCAAGCGGCAGGCGGTGGCCATCATATTCACTGATGACTCGGCACGTACCTTCACCT GCGACTCAGAGCTAGAGGCAGAGGAGTGGTACAAGACACTATCTGTGGAGTGTCTGGGGTCCCGCCTCAA CGACATCAGTCTGGGAGAACCTGACCTCCTGGCCCCAGGGGTGCAGTGTGAACAGACAGATCGCTTCAAT GTCTTCCTGCTGCCCTGCCCCAACCTGGACGTGTATGGCGAGTGCAAGCTGCAGATCACCCACGAGAACA TCTACCTCTGGGACATCCACAACCCCCGTGTGAAGCTCGTCTCGTGGCCCCTCTGCTCACTGCGCCGCTA TGGCCGGGATGCCACACGCTTTACCTTCGAGGCTGGCCGGATGTGTGATGCTGGGGAAGGACTCTATACC TTCCAGACACAAGAGGGGGAGCAGATTTACCAGCGCGTCCACAGTGCCACCCTGGCCATCGCAGAGCAGC ACAAGCGGGTCCTGCTGGAAATGGAGAAGAACGTGAGGCTGCTGAACAAGGGCACGGAACATTACTCGTA TCCCTGCACACCCACGACCATGCTGCCGCGCAGTGCCTACTGGCACCACATCACTGGTTCCCAGAACATC GCCGAAGCCTCCAGCTATGCTGGTGAGGGGTATGGGGCAGCCCAGGCCAGCTCGGAAACAGACCTCCTCA ACAGATTCATCCTGCTAAAGCCAAAGCCCAGCCAGGGGGACAGCAGTGAGGCCAAGACCCCATCCCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG202561 representing NM_018110
Red=Cloning site Green=Tags(s) MATNFSDIVKQGYVKMKSRKLGIYRRCWLVFRKSSSKGPQRLEKYPDEKSVCLRGCPKVTEISNVKCVTR LPKETKRQAVAIIFTDDSARTFTCDSELEAEEWYKTLSVECLGSRLNDISLGEPDLLAPGVQCEQTDRFN VFLLPCPNLDVYGECKLQITHENIYLWDIHNPRVKLVSWPLCSLRRYGRDATRFTFEAGRMCDAGEGLYT FQTQEGEQIYQRVHSATLAIAEQHKRVLLEMEKNVRLLNKGTEHYSYPCTPTTMLPRSAYWHHITGSQNI AEASSYAGEGYGAAQASSETDLLNRFILLKPKPSQGDSSEAKTPSQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018110 |
| ORF Size | 978 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018110.5 |
| RefSeq Size | 2727 bp |
| RefSeq ORF | 981 bp |
| Locus ID | 55715 |
| UniProt ID | Q8TEW6 |
| Domains | PH, IRS |
| Protein Families | Druggable Genome |
| Gene Summary | DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK4 functions in RET-mediated neurite outgrowth and plays a positive role in activation of the MAP kinase pathway (By similarity). Putative link with downstream effectors of RET in neuronal differentiation. May be involved in the regulation of the immune response induced by T-cells.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202561 | DOK4 (Myc-DDK-tagged)-Human docking protein 4 (DOK4) |
CNY 2,400.00 |
|
| RC202561L3 | Lenti ORF clone of Human docking protein 4 (DOK4), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202561L4 | Lenti ORF clone of Human docking protein 4 (DOK4), mGFP tagged |
CNY 5,890.00 |
|
| SC319110 | DOK4 (untagged)-Human docking protein 4 (DOK4) |
CNY 2,400.00 |
