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CRB3 (NM_174881) Human Tagged ORF Clone
CAT＃: RG203839
- TrueORF®
CRB3 (tGFP-tagged) - Human crumbs homolog 3 (Drosophila) (CRB3), transcript variant 3
ORF Plasmid: DDK
"NM_174881" in other vectors (4)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG203839 representing NM_174881
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAACCCCGGGCTGGGGCTGCTTCTGGCGCTGGGCCTGCCGTTCCTGCTGGCCCGCTGGGGCCGAG CCTGGGGGCAAATACAGACCACTTCTGCAAATGAGAATAGCACTGTTTTGCCTTCATCCACCAGCTCCAG CTCCGATGGCAACCTGCGTCCGGAAGCCATCACTGCTATCATCGTGGTCTTCTCCCTCTTGGCTGCCTTG CTCCTGGCTGTGGGGCTGGCACTGTTGGTGCGGAAGCTTCGGGAGAAGCGGCAGACGGAGGGCACCTACC GGCCCAGTAGCGAGGAGCAGTTCTCCCATGCAGCCGAGGCCCGGGCCCCTCAGGACTCCAAGGAGACGGT GCAGGGCTGCCTGCCCATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG203839 representing NM_174881
Red=Cloning site Green=Tags(s) MANPGLGLLLALGLPFLLARWGRAWGQIQTTSANENSTVLPSSTSSSSDGNLRPEAITAIIVVFSLLAAL LLAVGLALLVRKLREKRQTEGTYRPSSEEQFSHAAEARAPQDSKETVQGCLPI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_174881 |
| ORF Size | 369 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_174881.1, NP_777377.1 |
| RefSeq Size | 746 bp |
| RefSeq ORF | 372 bp |
| Locus ID | 92359 |
| UniProt ID | Q9BUF7 |
| Protein Families | Transmembrane |
| Protein Pathways | Tight junction |
| Gene Summary | This gene encodes a member of the Crumbs family of proteins. This gene is widely expressed in epithelial tissues where the encoded protein isoforms play various roles such as the control of cytokinesis and ciliogenesis or the formation of tight junctions. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203839 | CRB3 (Myc-DDK-tagged)-Human crumbs homolog 3 (Drosophila) (CRB3), transcript variant 3 |
CNY 1,200.00 |
|
| RC203839L3 | Lenti ORF clone of Human crumbs homolog 3 (Drosophila) (CRB3), transcript variant 3, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203839L4 | Lenti ORF clone of Human crumbs homolog 3 (Drosophila) (CRB3), transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
| SC123336 | CRB3 (untagged)-Human crumbs homolog 3 (Drosophila) (CRB3), transcript variant 3 |
CNY 1,200.00 |
