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RSK1 p90 (RPS6KA1) (NM_002953) Human Tagged ORF Clone
CAT＃: RG204874
- TrueORF®
RPS6KA1 (tGFP-tagged) - Human ribosomal protein S6 kinase, 90kDa, polypeptide 1 (RPS6KA1), transcript variant 1
ORF Plasmid: DDK
"NM_002953" in other vectors (7)
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CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | HU-1; MAPKAPK1; MAPKAPK1A; p90Rsk; RSK; RSK1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG204874 representing NM_002953
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGCTCGCCCAGCTCAAGGAGCCCTGGCCGCTCATGGAGCTAGTGCCGCTGGACCCGGAGAATGGAC AGACCTCAGGGGAAGAAGCTGGACTTCAGCCGTCCAAGGATGAGGGCGTCCTCAAGGAGATCTCCATCAC GCACCACGTCAAGGCTGGCTCTGAGAAGGCTGATCCATCCCATTTCGAGCTCCTCAAGGTTCTGGGCCAG GGATCCTTTGGCAAAGTCTTCCTGGTGCGGAAAGTCACCCGGCCTGACAGTGGGCACCTGTATGCTATGA AGGTGCTGAAGAAGGCAACGCTGAAAGTACGTGACCGCGTCCGGACCAAGATGGAGAGAGACATCCTGGC TGATGTAAATCACCCATTCGTGGTGAAGCTGCACTATGCCTTCCAGACCGAGGGCAAGCTCTATCTCATT CTGGACTTCCTGCGTGGTGGGGACCTCTTCACCCGGCTCTCAAAAGAGGTGATGTTCACGGAGGAGGATG TGAAGTTTTACCTGGCCGAGCTGGCTCTGGGCCTGGATCACCTGCACAGCCTGGGTATCATTTACAGAGA CCTCAAGCCTGAGAACATCCTTCTGGATGAGGAGGGCCACATCAAACTCACTGACTTTGGCCTGAGCAAA GAGGCCATTGACCACGAGAAGAAGGCCTATTCTTTCTGCGGGACAGTGGAGTACATGGCCCCTGAGGTCG TCAACCGCCAGGGCCACTCCCATAGTGCGGACTGGTGGTCCTATGGGGTGTTGATGTTTGAGATGCTGAC GGGCTCCCTGCCCTTCCAGGGGAAGGACCGGAAGGAGACCATGACACTGATTCTGAAGGCGAAGCTAGGC ATGCCCCAGTTTCTGAGCACTGAAGCCCAGAGCCTCTTGCGGGCCCTGTTCAAGCGGAATCCTGCCAACC GGCTCGGCTCCGGCCCTGATGGGGCAGAGGAAATCAAGCGGCATGTCTTCTACTCCACCATTGACTGGAA TAAGCTATACCGTCGTGAGATCAAGCCACCCTTCAAGCCAGCAGTGGCTCAGCCTGATGACACCTTCTAC TTTGACACCGAGTTCACGTCCCGCACACCCAAGGATTCCCCAGGCATCCCCCCCAGCGCTGGGGCCCATC AGCTGTTCCGGGGCTTCAGCTTCGTGGCCACCGGCCTGATGGAAGACGACGGCAAGCCTCGTGCCCCGCA GGCACCCCTGCACTCGGTGGTACAGCAACTCCATGGGAAGAACCTGGTTTTTAGTGACGGCTACGTGGTA AAGGAGACAATTGGTGTGGGCTCCTACTCTGAGTGCAAGCGCTGTGTCCACAAGGCCACCAACATGGAGT ATGCTGTCAAGGTCATTGATAAGAGCAAGCGGGATCCTTCAGAAGAGATTGAGATTCTTCTGCGGTATGG CCAGCACCCCAACATCATCACTCTGAAAGATGTGTATGATGATGGCAAACACGTGTACCTGGTGACAGAG CTGATGCGGGGTGGGGAGCTGCTGGACAAGATCCTGCGGCAGAAGTTCTTCTCAGAGCGGGAGGCCAGCT TTGTCCTGCACACCATTGGCAAAACTGTGGAGTATCTGCACTCACAGGGGGTTGTGCACAGGGACCTGAA GCCCAGCAACATCCTGTATGTGGACGAGTCCGGGAATCCCGAGTGCCTGCGCATCTGTGACTTTGGTTTT GCCAAACAGCTGCGGGCTGAGAATGGGCTCCTCATGACACCTTGCTACACAGCCAACTTTGTGGCGCCTG AGGTGCTGAAGCGCCAGGGCTACGATGAAGGCTGCGACATCTGGAGCCTGGGCATTCTGCTGTACACCAT GCTGGCAGGATATACTCCATTTGCCAACGGTCCCAGTGACACACCAGAGGAAATCCTAACCCGGATCGGC AGTGGGAAGTTTACCCTCAGTGGGGGAAATTGGAACACAGTTTCAGAGACAGCCAAGGACCTGGTGTCCA AGATGCTACACGTGGATCCCCACCAGCGCCTCACAGCTAAGCAGGTTCTGCAGCATCCATGGGTCACCCA GAAAGACAAGCTTCCCCAAAGCCAGCTGTCCCACCAGGACCTACAGCTTGTGAAGGGAGCCATGGCTGCC ACGTACTCCGCACTCAACAGCTCCAAGCCCACCCCCCAGCTGAAGCCCATCGAGTCATCCATCCTGGCCC AGCGGCGAGTGAGGAAGTTGCCATCCACCACCCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG204874 representing NM_002953
Red=Cloning site Green=Tags(s) MPLAQLKEPWPLMELVPLDPENGQTSGEEAGLQPSKDEGVLKEISITHHVKAGSEKADPSHFELLKVLGQ GSFGKVFLVRKVTRPDSGHLYAMKVLKKATLKVRDRVRTKMERDILADVNHPFVVKLHYAFQTEGKLYLI LDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALGLDHLHSLGIIYRDLKPENILLDEEGHIKLTDFGLSK EAIDHEKKAYSFCGTVEYMAPEVVNRQGHSHSADWWSYGVLMFEMLTGSLPFQGKDRKETMTLILKAKLG MPQFLSTEAQSLLRALFKRNPANRLGSGPDGAEEIKRHVFYSTIDWNKLYRREIKPPFKPAVAQPDDTFY FDTEFTSRTPKDSPGIPPSAGAHQLFRGFSFVATGLMEDDGKPRAPQAPLHSVVQQLHGKNLVFSDGYVV KETIGVGSYSECKRCVHKATNMEYAVKVIDKSKRDPSEEIEILLRYGQHPNIITLKDVYDDGKHVYLVTE LMRGGELLDKILRQKFFSEREASFVLHTIGKTVEYLHSQGVVHRDLKPSNILYVDESGNPECLRICDFGF AKQLRAENGLLMTPCYTANFVAPEVLKRQGYDEGCDIWSLGILLYTMLAGYTPFANGPSDTPEEILTRIG SGKFTLSGGNWNTVSETAKDLVSKMLHVDPHQRLTAKQVLQHPWVTQKDKLPQSQLSHQDLQLVKGAMAA TYSALNSSKPTPQLKPIESSILAQRRVRKLPSTTL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002953 |
| ORF Size | 2205 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002953.2 |
| RefSeq Size | 3199 bp |
| RefSeq ORF | 2208 bp |
| Locus ID | 6195 |
| UniProt ID | Q15418 |
| Domains | pkinase, S_TK_X, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Long-term potentiation, MAPK signaling pathway, mTOR signaling pathway, Neurotrophin signaling pathway, Oocyte meiosis, Progesterone-mediated oocyte maturation |
| Gene Summary | This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204874 | RPS6KA1 (Myc-DDK-tagged)-Human ribosomal protein S6 kinase, 90kDa, polypeptide 1 (RPS6KA1), transcript variant 1 |
CNY 5,384.00 |
|
| RC204874L1 | Lenti ORF clone of Human ribosomal protein S6 kinase, 90kDa, polypeptide 1 (RPS6KA1), transcript variant 1, Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC204874L2 | Lenti ORF clone of Human ribosomal protein S6 kinase, 90kDa, polypeptide 1 (RPS6KA1), transcript variant 1, mGFP tagged |
CNY 7,700.00 |
|
| RC204874L3 | Lenti ORF clone of Human ribosomal protein S6 kinase, 90kDa, polypeptide 1 (RPS6KA1), transcript variant 1, Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC204874L4 | Lenti ORF clone of Human ribosomal protein S6 kinase, 90kDa, polypeptide 1 (RPS6KA1), transcript variant 1, mGFP tagged |
CNY 7,700.00 |
|
| SC118295 | RPS6KA1 (untagged)-Human ribosomal protein S6 kinase, 90kDa, polypeptide 1 (RPS6KA1), transcript variant 1 |
CNY 2,950.00 |
|
| SC323333 | RPS6KA1 (untagged)-Kinase deficient mutant (K94M) of Human ribosomal protein S6 kinase, 90kDa, polypeptide 1 (RPS6KA1), transcript variant 1 |
CNY 5,392.00 |
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