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HIBADH (NM_152740) Human Tagged ORF Clone
CAT＃: RG207125
- TrueORF®
HIBADH (tGFP-tagged) - Human 3-hydroxyisobutyrate dehydrogenase (HIBADH)
ORF Plasmid: DDK
"NM_152740" in other vectors (6)
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CNY 7,088.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | NS5ATP1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG207125 representing NM_152740
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCCTCCTTACGGCTCCTCGGAGCTGCCTCCGGTCTCCGGTACTGGAGCCGGCGGCTGCGGCCGG CAGCCGGCAGCTTTGCAGCGGTGTGTTCTAGGTCAGTGGCTTCAAAGACTCCAGTTGGATTCATTGGACT GGGCAACATGGGGAATCCAATGGCAAAAAATCTCATGAAACATGGCTATCCACTTATTATTTATGATGTG TTCCCTGATGCCTGCAAAGAGTTTCAAGATGCAGGTGAACAGGTAGTATCTTCCCCAGCAGATGTTGCTG AAAAAGCTGACAGAATTATTACAATGCTGCCCACCAGTATCAATGCAATAGAAGCTTATTCCGGAGCAAA TGGGATTCTAAAAAAAGTGAAGAAGGGCTCATTATTAATAGATTCCAGCACTATTGATCCTGCAGTTTCA AAAGAATTGGCCAAAGAAGTTGAGAAAATGGGAGCAGTTTTCATGGATGCCCCTGTTTCTGGTGGTGTAG GAGCTGCACGATCTGGGAACCTCACGTTTATGGTGGGAGGAGTTGAAGATGAATTTGCTGCTGCCCAAGA GTTGCTGGGGTGCATGGGCTCCAACGTGGTGTACTGTGGAGCTGTTGGGACTGGGCAGGCGGCAAAGATC TGCAACAACATGCTGTTAGCTATTAGTATGATTGGAACTGCTGAAGCTATGAATCTTGGAATCAGGTTAG GGCTTGACCCAAAACTACTGGCTAAAATCCTAAATATGAGCTCAGGACGGTGTTGGTCAAGTGACACTTA TAATCCTGTACCTGGAGTGATGGATGGCGTTCCCTCGGCTAATAACTATCAGGGTGGATTTGGAACAACA CTCATGGCTAAGGATCTGGGATTGGCACAAGACTCTGCTACCAGCACAAAGAGCCCAATCCTTCTTGGCA GTCTGGCCCATCAGATCTACAGGATGATGTGTGCAAAGGGCTACTCAAAGAAAGACTTCTCATCCGTGTT CCAGTTCCTACGAGAGGAGGAGACCTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG207125 representing NM_152740
Red=Cloning site Green=Tags(s) MAASLRLLGAASGLRYWSRRLRPAAGSFAAVCSRSVASKTPVGFIGLGNMGNPMAKNLMKHGYPLIIYDV FPDACKEFQDAGEQVVSSPADVAEKADRIITMLPTSINAIEAYSGANGILKKVKKGSLLIDSSTIDPAVS KELAKEVEKMGAVFMDAPVSGGVGAARSGNLTFMVGGVEDEFAAAQELLGCMGSNVVYCGAVGTGQAAKI CNNMLLAISMIGTAEAMNLGIRLGLDPKLLAKILNMSSGRCWSSDTYNPVPGVMDGVPSANNYQGGFGTT LMAKDLGLAQDSATSTKSPILLGSLAHQIYRMMCAKGYSKKDFSSVFQFLREEETF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_152740 |
| ORF Size | 1008 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_152740.4 |
| RefSeq Size | 2006 bp |
| RefSeq ORF | 1011 bp |
| Locus ID | 11112 |
| UniProt ID | P31937 |
| Domains | NAD_binding_2 |
| Protein Pathways | Metabolic pathways, Valine, leucine and isoleucine degradation |
| Gene Summary | This gene encodes a mitochondrial 3-hydroxyisobutyrate dehydrogenase enzyme. The encoded protein plays a critical role in the catabolism of L-valine by catalyzing the oxidation of 3-hydroxyisobutyrate to methylmalonate semialdehyde. [provided by RefSeq, Nov 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207125 | HIBADH (Myc-DDK-tagged)-Human 3-hydroxyisobutyrate dehydrogenase (HIBADH) |
CNY 5,488.00 |
|
| RC207125L1 | Lenti ORF clone of Human 3-hydroxyisobutyrate dehydrogenase (HIBADH), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207125L2 | Lenti ORF clone of Human 3-hydroxyisobutyrate dehydrogenase (HIBADH), mGFP tagged |
CNY 7,888.00 |
|
| RC207125L3 | Lenti ORF clone of Human 3-hydroxyisobutyrate dehydrogenase (HIBADH), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207125L4 | Lenti ORF clone of Human 3-hydroxyisobutyrate dehydrogenase (HIBADH), mGFP tagged |
CNY 5,890.00 |
|
| SC100318 | HIBADH (untagged)-Human 3-hydroxyisobutyrate dehydrogenase (HIBADH) |
CNY 2,950.00 |
