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HERC4 (NM_015601) Human Tagged ORF Clone
CAT＃: RG208071
- TrueORF®
HERC4 (tGFP-tagged) - Human hect domain and RLD 4 (HERC4), transcript variant 2
ORF Plasmid: DDK
"NM_015601" in other vectors (6)
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CNY 6,270.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG208071 representing NM_015601
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGTGCTGGGGAAATGCATCCTTTGGGCAGCTAGGTTTGGGTGGAATTGATGAAGAAATTGTACTAG AGCCCAGAAAAAGTGACTTCTTTATAAATAAAAGGGTCCGAGATGTAGGATGTGGACTCAGACATACTGT GTTTGTTCTGGATGATGGAACAGTGTACACATGTGGATGTAATGATCTAGGACAGCTAGGTCATGAAAAA TCCAGAAAGAAACCAGAGCAGGTTGTTGCCCTGGATGCCCAAAATATTGTAGCTGTTTCATGTGGAGAAG CTCATACGTTAGCGCTAAATGACAAAGGCCAGGTGTATGCTTGGGGTCTCGATTCTGATGGACAGCTTGG CCTGGTAGGATCAGAGGAATGCATCAGAGTACCCAGAAATATTAAAAGTTTGTCAGATATCCAGATTGTA CAGGTTGCTTGTGGTTACTATCATTCACTTGCACTTTCTAAAGCAAGTGAAGTCTTCTGTTGGGGACAGA ATAAATATGGCCAATTGGGTTTAGGTACTGACTGTAAAAAGCAAACTTCACCGCAGCTGCTTAAGTCTTT GCTTGGAATCCCTTTCATGCAAGTTGCAGCAGGAGGAGCCCATAGTTTTGTACTCACCCTTTCTGGAGCT ATCTTTGGATGGGGACGCAACAAGTTTGGTCAGCTAGGTCTTAATGATGAAAATGATAGGTATGTTCCTA ATTTACTAAAGTCACTAAGATCTCAGAAAATAGTTTATATTTGTTGTGGAGAAGATCATACTGCTGCTCT AACCAAGGAAGGTGGAGTGTTTACTTTTGGAGCTGGAGGGTATGGTCAGTTGGGCCATAATTCTACCAGT CATGAAATAAACCCAAGGAAAGTTTTTGAACTTATGGGAAGCATTGTCACTGAGATTGCTTGTGGACGGC AGCACACTTCTGCTTTTGTTCCTTCATCAGGACGAATTTACTCTTTTGGGCTTGGTGGTAATGGGCAGCT GGGAACCGGTTCAACAAGCAACAGGAAAAGCCCCTTTACTGTAAAAGGAAATTGGTACCCCTATAATGGG CAGTGTCTACCAGATATTGATTCTGAAGAATATTTCTGTGTAAAAAGAATTTTCTCAGGGGGAGATCAAA GCTTTTCACATTACTCTAGTCCCCAGAACTGTGGGCCACCAGATGACTTCAGATGTCCCAATCCGACAAA GCAGATCTGGACAGTGAATGAAGCTCTAATTCAGAAATGGCTGAGCTATCCTTCTGGAAGGTTTCCTGTG GAGATAGCCAATGAGATAGATGGAACGTTTTCTTCCTCTGGTTGCCTAAATGGAAGTTTTTTAGCTGTTA GCAATGATGATCACTATAGAACAGGTACCAGATTTTCAGGGGTTGATATGAATGCTGCTAGGCTTTTATT CCACAAACTTATACAACCTGATCATCCGCAGATATCTCAGCAGGTGGCAGCTAGTTTGGAAAAGAATCTT ATTCCTAAACTGACTAGCTCCTTACCTGATGTTGAAGCATTGAGGTTTTATCTTACTCTACCAGAATGTC CCCTGATGAGTGATTCCAACAATTTCACAACAATAGCAATTCCCTTTGGTACAGCTCTTGTGAACCTAGA AAAGGCACCACTGAAAGTACTTGAAAACTGGTGGTCAGTACTTGAACCTCCACTATTCCTCAAGATAGTA GAACTTTTTAAGGAAGTTGTGGTACATCTTTTGAAACTCTACAAGATCGGTATTCCCCCTTCTGAAAGAA GAATTTTCAACAGTTTTCTTCATACTGCATTAAAGGTTTTAGAAATACTACATAGGGTAAATGAGAAAAT GGGACAGATTATACAGTATGATAAATTTTATATACATGAAGTACAAGAATTGATAGACATAAGAAATGAT TATATCAACTGGGTCCAACAGCAGGCCTATGGAATGTTGGCAGATATCCCTGTTACAATCTGTACATATC CATTTGTATTTGATGCCCAAGCAAAAACTACTCTGTTACAGACCGATGCAGTCTTACAGATGCAGATGGC TATTGATCAGGCCCACAGGCAGAATGTCTCCTCTCTTTTTCTCCCAGTGATTGAATCTGTGAATCCCTGC TTAATTCTAGTGGTGCGTAGAGAAAATATTGTAGGAGATGCAATGGAAGTCCTTAGGAAAACAAAGAACA TAGATTACAAGAAGCCACTCAAGGTTATATTTGTTGGAGAAGATGCTGTGGATGCAGGAGGGGTGCGCAA AGAATTTTTCTTGCTCATCATGAGGGAATTATTGGATCCTAAATACGGCATGTTTAGGTATTATGAAGAT TCCAGGCTCATTTGGTTTTCTGATAAGACATTTGAAGACAGTGATTTGTTCCATTTGATTGGTGTTATCT GTGGCTTAGCAATTTATAATTGTACCATTGTGGACCTCCATTTTCCTTTGGCTTTATATAAGAAACTACT GAAAAAGAAGCCATCCTTGGATGATTTGAAAGAACTAATGCCTGATGTTGGGAGAAGCATGCAACAGTTA CTGGATTATCCAGAAGATGACATAGAGGAAACATTTTGTCTTAATTTTACGATCACAGTTGAAAACTTTG GTGCAACAGAAGTGAAAGAGCTGGTTCTAAATGGTGCAGACACAGCTGTTAACAAACAAAATCGGCAAGA GTTTGTCGATGCTTATGTGGATTACATATTCAATAAATCAGTGGCTTCCTTATTTGATGCTTTTCATGCG GGCTTTCATAAGGTCTGTGGAGGAAAAGTCCTTCTGCTCTTTCAGCCTAATGAACTACAAGCAATGGTCA TTGGAAATACAAATTATGATTGGAAGGAACTGGAAAAGAATACAGAATACAAAGGGGAATATTGGGCAGA ACATCCTACGATAAAAATTTTTTGGGAAGTATTTCACGAATTACCATTGGAAAAGAAGAAACAGTTTCTG TTATTTTTGACAGGTAGTGATCGCATTCCTATTCTTGGTATGAAGAGTCTGAAACTAGTCATCCAGTCCA CAGGAGGTGGTGAGGAGTATCTCCCAGTTTCCCATACTTGTTTTAATCTTCTGGATCTTCCAAAATATAC AGAAAAAGAAACTCTACGCTCTAAACTGATCCAAGCTATTGATCACAATGAAGGCTTCAGTTTAATA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG208071 representing NM_015601
Red=Cloning site Green=Tags(s) MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKRVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEK SRKKPEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLVGSEECIRVPRNIKSLSDIQIV QVACGYYHSLALSKASEVFCWGQNKYGQLGLGTDCKKQTSPQLLKSLLGIPFMQVAAGGAHSFVLTLSGA IFGWGRNKFGQLGLNDENDRYVPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTS HEINPRKVFELMGSIVTEIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWYPYNG QCLPDIDSEEYFCVKRIFSGGDQSFSHYSSPQNCGPPDDFRCPNPTKQIWTVNEALIQKWLSYPSGRFPV EIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTRFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNL IPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIV ELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHRVNEKMGQIIQYDKFYIHEVQELIDIRND YINWVQQQAYGMLADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPC LILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYED SRLIWFSDKTFEDSDLFHLIGVICGLAIYNCTIVDLHFPLALYKKLLKKKPSLDDLKELMPDVGRSMQQL LDYPEDDIEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHA GFHKVCGGKVLLLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFL LFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015601 |
| ORF Size | 3147 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015601.4 |
| RefSeq Size | 3842 bp |
| RefSeq ORF | 3150 bp |
| Locus ID | 26091 |
| UniProt ID | Q5GLZ8 |
| Protein Families | Druggable Genome |
| Protein Pathways | Ubiquitin mediated proteolysis |
| Gene Summary | HERC4 belongs to the HERC family of ubiquitin ligases, all of which contain a HECT domain and at least 1 RCC1 (MIM 179710)-like domain (RLD). The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before transferring it to a substrate, and the RLD is predicted to act as a guanine nucleotide exchange factor for small G proteins (Hochrainer et al., 2005 [PubMed 15676274]).[supplied by OMIM, Mar 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208071 | HERC4 (Myc-DDK-tagged)-Human hect domain and RLD 4 (HERC4), transcript variant 2 |
CNY 7,304.00 |
|
| RC208071L1 | Lenti ORF clone of Human hect domain and RLD 4 (HERC4), transcript variant 2, Myc-DDK-tagged |
CNY 9,704.00 |
|
| RC208071L2 | Lenti ORF clone of Human hect domain and RLD 4 (HERC4), transcript variant 2, mGFP tagged |
CNY 7,600.00 |
|
| RC208071L3 | Lenti ORF clone of Human hect domain and RLD 4 (HERC4), transcript variant 2, Myc-DDK-tagged |
CNY 7,600.00 |
|
| RC208071L4 | Lenti ORF clone of Human hect domain and RLD 4 (HERC4), transcript variant 2, mGFP tagged |
CNY 7,600.00 |
|
| SC128201 | HERC4 (untagged)-Human hect domain and RLD 4 (HERC4), transcript variant 2 |
CNY 7,312.00 |
