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GUCY1B1 (NM_000857) Human Tagged ORF Clone
CAT＃: RG208205
- TrueORF®
GUCY1B3 (tGFP-tagged) - Human guanylate cyclase 1, soluble, beta 3 (GUCY1B3)
ORF Plasmid: DDK
"NM_000857" in other vectors (7)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | GC-S-beta-1; GC-SB3; GUC1B3; GUCB3; GUCSB3; GUCY1B3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG208205 representing NM_000857
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACGGATTTGTGAATCACGCCCTGGAGTTGCTGGTGATCCGCAATTACGGCCCCGAGGTGTGGGAAG ACATCAAAAAAGAGGCACAGTTAGATGAAGAAGGACAGTTTCTTGTCAGAATAATATATGATGACTCCAA AACTTATGATTTGGTTGCTGCTGCAAGCAAAGTCCTCAATCTCAATGCTGGAGAAATCCTCCAAATGTTT GGGAAGATGTTTTTCGTCTTTTGCCAAGAATCTGGTTATGATACAATCTTGCGTGTCCTGGGCTCTAATG TCAGAGAATTTCTACAGAACCTTGATGCTCTGCACGACCACCTTGCTACCATCTACCCAGGAATGCGTGC ACCTTCCTTTAGGTGCACTGATGCAGAAAAGGGCAAAGGACTCATTTTGCACTACTACTCAGAGAGAGAA GGACTTCAGGATATTGTCATTGGAATCATCAAAACAGTGGCACAACAAATCCATGGCACTGAAATAGACA TGAAGGTTATTCAGCAAAGAAATGAAGAATGTGATCATACTCAATTTTTAATTGAAGAAAAAGAGTCAAA AGAAGAGGATTTTTATGAAGATCTTGACAGATTTGAAGAAAATGGTACCCAGGAATCACGCATCAGCCCA TATACATTCTGCAAAGCTTTTCCTTTTCATATAATATTTGACCGGGACCTAGTGGTCACTCAGTGTGGCA ATGCTATATACAGAGTTCTCCCCCAGCTCCAGCCTGGGAATTGCAGCCTTCTGTCTGTCTTCTCGCTGGT TCGTCCTCATATTGATATTAGTTTCCATGGGATCCTTTCTCACATCAATACTGTTTTTGTATTGAGAAGC AAGGAAGGATTGTTGGATGTGGAGAAATTAGAATGTGAGGATGAACTGACTGGGACTGAGATCAGCTGCT TACGTCTCAAGGGTCAAATGATCTACTTACCTGAAGCAGATAGCATACTTTTTCTATGTTCACCAAGTGT CATGAACCTGGACGATTTGACAAGGAGAGGGCTGTATCTAAGTGACATCCCTCTGCATGATGCCACACGC GATCTTGTTCTTTTGGGAGAACAATTTAGAGAGGAATACAAACTCACCCAAGAACTGGAAATCCTCACTG ACAGGCTACAGCTCACGTTAAGAGCCCTGGAAGATGAAAAGAAAAAGACAGACACATTGCTGTATTCTGT CCTTCCTCCGTCTGTTGCCAATGAGCTGCGGCACAAGCGTCCAGTGCCTGCCAAAAGATATGACAATGTG ACCATCCTCTTTAGTGGCATTGTGGGCTTCAATGCTTTCTGTAGCAAGCATGCATCTGGAGAAGGAGCCA TGAAGATCGTCAACCTCCTCAACGACCTCTACACCAGATTTGACACACTGACTGATTCCCGGAAAAACCC ATTTGTTTATAAGGTGGAGACTGTTGGTGACAAGTATATGACAGTGAGTGGTTTACCAGAGCCATGCATT CACCATGCACGATCCATCTGCCACCTGGCCTTGGACATGATGGAAATTGCTGGCCAGGTTCAAGTAGATG GTGAATCTGTTCAGATAACAATAGGGATACACACTGGAGAGGTAGTTACAGGTGTCATAGGACAGCGGAT GCCTCGATACTGTCTTTTTGGGAATACTGTCAACCTCACAAGCCGAACAGAAACCACAGGAGAAAAGGGA AAAATAAATGTGTCTGAATATACATACAGATGTCTTATGTCTCCAGAAAATTCAGATCCACAATTCCACT TGGAGCACAGAGGCCCAGTGTCCATGAAGGGCAAAAAAGAACCAATGCAAGTTTGGTTTCTATCCAGAAA AAATACAGGAACAGAGGAAACAAAGCAGGATGATGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG208205 representing NM_000857
Red=Cloning site Green=Tags(s) MYGFVNHALELLVIRNYGPEVWEDIKKEAQLDEEGQFLVRIIYDDSKTYDLVAAASKVLNLNAGEILQMF GKMFFVFCQESGYDTILRVLGSNVREFLQNLDALHDHLATIYPGMRAPSFRCTDAEKGKGLILHYYSERE GLQDIVIGIIKTVAQQIHGTEIDMKVIQQRNEECDHTQFLIEEKESKEEDFYEDLDRFEENGTQESRISP YTFCKAFPFHIIFDRDLVVTQCGNAIYRVLPQLQPGNCSLLSVFSLVRPHIDISFHGILSHINTVFVLRS KEGLLDVEKLECEDELTGTEISCLRLKGQMIYLPEADSILFLCSPSVMNLDDLTRRGLYLSDIPLHDATR DLVLLGEQFREEYKLTQELEILTDRLQLTLRALEDEKKKTDTLLYSVLPPSVANELRHKRPVPAKRYDNV TILFSGIVGFNAFCSKHASGEGAMKIVNLLNDLYTRFDTLTDSRKNPFVYKVETVGDKYMTVSGLPEPCI HHARSICHLALDMMEIAGQVQVDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKG KINVSEYTYRCLMSPENSDPQFHLEHRGPVSMKGKKEPMQVWFLSRKNTGTEETKQDDD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000857 |
| ORF Size | 1857 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000857.1, NP_000848.1 |
| RefSeq Size | 2443 bp |
| RefSeq ORF | 1860 bp |
| Locus ID | 2983 |
| UniProt ID | Q02153 |
| Domains | CYCc |
| Protein Families | Druggable Genome |
| Protein Pathways | Gap junction, Long-term depression, Purine metabolism, Vascular smooth muscle contraction |
| Gene Summary | This gene encodes the beta subunit of the soluble guanylate cyclase (sGC), which catalyzes the conversion of GTP (guanosine triphosphate) to cGMP (cyclic guanosine monophosphate). The encoded protein contains an HNOX domain, which serves as a receptor for ligands such as nitric oxide, oxygen and nitrovasodilator drugs. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208205 | GUCY1B3 (Myc-DDK-tagged)-Human guanylate cyclase 1, soluble, beta 3 (GUCY1B3) |
CNY 3,990.00 |
|
| RC208205L1 | Lenti ORF clone of Human guanylate cyclase 1, soluble, beta 3 (GUCY1B3), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208205L2 | Lenti ORF clone of Human guanylate cyclase 1, soluble, beta 3 (GUCY1B3), mGFP tagged |
CNY 5,890.00 |
|
| RC208205L3 | Lenti ORF clone of Human guanylate cyclase 1, soluble, beta 3 (GUCY1B3), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208205L4 | Lenti ORF clone of Human guanylate cyclase 1, soluble, beta 3 (GUCY1B3), mGFP tagged |
CNY 5,890.00 |
|
| SC119659 | GUCY1B3 (untagged)-Human guanylate cyclase 1, soluble, beta 3 (GUCY1B3) |
CNY 2,950.00 |
|
| SC322151 | GUCY1B3 (untagged)-Human guanylate cyclase 1, soluble, beta 3 (GUCY1B3) |
CNY 2,950.00 |
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