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CHST14 (NM_130468) Human Tagged ORF Clone
CAT＃: RG208428
- TrueORF®
CHST14 (tGFP-tagged) - Human carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 14 (CHST14)
ORF Plasmid: DDK
"NM_130468" in other vectors (6)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ATCS; D4ST1; EDSMC1; HNK1ST |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG208428 representing NM_130468
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCCCCCGCCCGCTGACCCCGCTGGCGGCCCCAAATGGCGCCGAGCCCCTGGGCCGGGCGCTGAGGC GGGCCCCTCTGGGCAGGGCCCGGGCGGGGCTGGGTGGGCCGCCCCTGCTGCTGCCGTCCATGCTGATGTT TGCGGTGATCGTGGCCTCCAGCGGGCTGCTGCTCATGATCGAGCGGGGCATCCTGGCCGAGATGAAGCCC CTGCCCCTGCACCCGCCCGGCCGCGAGGGCACAGCCTGGCGCGGGAAAGCCCCCAAGCCTGGGGGCCTGT CCCTCAGGGCTGGGGACGCGGACTTGCAAGTGCGGCAGGACGTCCGGAACAGGACCCTGCGGGCGGTGTG CGGACAGCCAGGCATGCCCCGGGACCCCTGGGACTTGCCGGTGGGGCAGCGGCGCACCCTGCTGCGCCAC ATCCTCGTAAGTGACCGTTACCGCTTCCTCTACTGCTACGTCCCCAAGGTGGCCTGCTCTAACTGGAAGC GGGTGATGAAGGTGCTGGCAGGCGTCCTGGACAGCGTGGACGTCCGCCTCAAGATGGACCACCGCAGTGA CCTGGTGTTCCTGGCCGACCTGCGGCCTGAGGAGATTCGCTACCGCCTGCAGCACTACTTTAAGTTCCTG TTTGTGCGGGAGCCCTTGGAACGCCTCCTCTCTGCCTACCGCAACAAGTTTGGCGAGATCCGAGAGTACC AGCAACGCTATGGGGCTGAGATAGTGAGGCGGTACAGGGCTGGAGCGGGGCCCAGCCCTGCAGGCGACGA TGTCACATTCCCCGAGTTCCTGAGATACCTGGTGGATGAGGACCCTGAGCGCATGAATGAGCATTGGATG CCCGTGTACCACCTGTGCCAGCCTTGTGCCGTGCACTATGACTTTGTGGGCTCCTATGAGAGGCTGGAGG CTGATGCAAATCAGGTGCTGGAGTGGGTACGGGCACCACCTCACGTCCGATTTCCAGCTCGCCAGGCCTG GTACCGGCCAGCCAGCCCCGAAAGCCTGCATTACCACTTGTGCAGTGCCCCCCGGGCCCTGCTGCAGGAT GTGCTGCCTAAGTATATCCTGGACTTCTCCCTCTTTGCCTACCCACTGCCTAATGTCACCAAGGAGGCGT GTCAGCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG208428 representing NM_130468
Red=Cloning site Green=Tags(s) MFPRPLTPLAAPNGAEPLGRALRRAPLGRARAGLGGPPLLLPSMLMFAVIVASSGLLLMIERGILAEMKP LPLHPPGREGTAWRGKAPKPGGLSLRAGDADLQVRQDVRNRTLRAVCGQPGMPRDPWDLPVGQRRTLLRH ILVSDRYRFLYCYVPKVACSNWKRVMKVLAGVLDSVDVRLKMDHRSDLVFLADLRPEEIRYRLQHYFKFL FVREPLERLLSAYRNKFGEIREYQQRYGAEIVRRYRAGAGPSPAGDDVTFPEFLRYLVDEDPERMNEHWM PVYHLCQPCAVHYDFVGSYERLEADANQVLEWVRAPPHVRFPARQAWYRPASPESLHYHLCSAPRALLQD VLPKYILDFSLFAYPLPNVTKEACQQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_130468 |
| ORF Size | 1128 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_130468.4 |
| RefSeq Size | 2156 bp |
| RefSeq ORF | 1131 bp |
| Locus ID | 113189 |
| UniProt ID | Q8NCH0 |
| Protein Pathways | Chondroitin sulfate biosynthesis |
| Gene Summary | This gene encodes a member of the HNK-1 family of sulfotransferases. The encoded protein transfers sulfate to the C-4 hydroxyl of N-acetylgalactosamine residues in dermatan sulfate. Mutations in this gene have been associated with adducted thumb-clubfoot syndrome.[provided by RefSeq, Mar 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208428 | CHST14 (Myc-DDK-tagged)-Human carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 14 (CHST14) |
CNY 3,656.00 |
|
| RC208428L1 | Lenti ORF clone of Human carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 14 (CHST14), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208428L2 | Lenti ORF clone of Human carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 14 (CHST14), mGFP tagged |
CNY 5,890.00 |
|
| RC208428L3 | Lenti ORF clone of Human carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 14 (CHST14), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208428L4 | Lenti ORF clone of Human carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 14 (CHST14), mGFP tagged |
CNY 5,890.00 |
|
| SC305893 | CHST14 (untagged)-Human carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 14 (CHST14) |
CNY 7,220.00 |
